LOCUS       NZ_CP083924             7669 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Enterococcus faecium isolate V1164 plasmid pV1164-4, complete
            sequence.
ACCESSION   NZ_CP083924
VERSION     NZ_CP083924.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA5367917
            Assembly: GCF_020162175.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Mollerup,S., Elmeskov,C., Gumpert,H., Pinholt,M., Sejersen,T.S.,
            Pedersen,M.S., Worning,P., Frees,D. and Westh,H.
  TITLE     Rapid in vivo development of resistance to daptomycin in
            vancomycin-resistant Enterococcus faecium due to genomic
            rearrangements
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   Mollerup,S., Elmeskov,C., Gumpert,H., Pinholt,M., Sejersen,T.S.,
            Pedersen,M.S., Worning,P., Frees,D. and Westh,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2021) Department of Clinical Microbiology,
            Copenhagen University Hospital Hvidovre, Kettegaard Alle 30,
            Hvidovre 2650, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-JAN-2019
            Assembly Method                   :: Unicycler v. 4.0.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 254.19410228420895x
            Sequencing Technology :: Illumina MiSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 01:58:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,998
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 45 of 127
            Pseudo Genes (incomplete)         :: 76 of 127
            Pseudo Genes (internal stop)      :: 35 of 127
            Pseudo Genes (multiple problems)  :: 26 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083924.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="V1164"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pV1164-4"
                     /geo_loc_name="Denmark"
                     /collection_date="2015"
     gene            join(7262..7669,1..81)
                     /locus_tag="LCG90_RS14795"
                     /old_locus_tag="LCG90_14820"
     CDS             join(7262..7669,1..81)
                     /locus_tag="LCG90_RS14795"
                     /old_locus_tag="LCG90_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_060763418.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKEA
                     VTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQQK
                     LLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            377..658
                     /locus_tag="LCG90_RS14800"
                     /old_locus_tag="LCG90_14825"
     CDS             377..658
                     /locus_tag="LCG90_RS14800"
                     /old_locus_tag="LCG90_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368064.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIENDF
                     DQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            660..1076
                     /locus_tag="LCG90_RS14805"
                     /old_locus_tag="LCG90_14830"
     CDS             660..1076
                     /locus_tag="LCG90_RS14805"
                     /old_locus_tag="LCG90_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368067.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(1831..2979)
                     /gene="tnpB"
                     /locus_tag="LCG90_RS14810"
                     /old_locus_tag="LCG90_14835"
     CDS             complement(1831..2979)
                     /gene="tnpB"
                     /locus_tag="LCG90_RS14810"
                     /old_locus_tag="LCG90_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(2996..3400)
                     /gene="tnpA"
                     /locus_tag="LCG90_RS14815"
                     /old_locus_tag="LCG90_14840"
     CDS             complement(2996..3400)
                     /gene="tnpA"
                     /locus_tag="LCG90_RS14815"
                     /old_locus_tag="LCG90_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            3581..4735
                     /locus_tag="LCG90_RS14820"
                     /old_locus_tag="LCG90_14845"
     CDS             3581..4735
                     /locus_tag="LCG90_RS14820"
                     /old_locus_tag="LCG90_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_002368070.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            4708..5151
                     /locus_tag="LCG90_RS14825"
                     /old_locus_tag="LCG90_14850"
     CDS             4708..5151
                     /locus_tag="LCG90_RS14825"
                     /old_locus_tag="LCG90_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223917876.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            5451..>5596
                     /locus_tag="LCG90_RS14830"
                     /old_locus_tag="LCG90_14855"
                     /pseudo=""
     CDS             5451..>5596
                     /locus_tag="LCG90_RS14830"
                     /old_locus_tag="LCG90_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            6284..7252
                     /locus_tag="LCG90_RS14835"
                     /old_locus_tag="LCG90_14860"
     CDS             6284..7252
                     /locus_tag="LCG90_RS14835"
                     /old_locus_tag="LCG90_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002368293.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     ADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIANFS
                     KEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELLQQLAE
                     LERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
                     /name="replication initiation protein"
     Typing          6285..7252
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaaaaagaac ccctttctgt tactgaagaa actcaaaaag gacaaaacaa ggagaaaaaa
       61 tggtggcaat tctggcaata acccaacaat ttttattcat aaaaattgtt gggttacttt
      121 ctttttttgc ttttacaagc atagcgacat gatctcacgt cgctattttt taaagagcat
      181 aaggaaacgc ggtatcactt tcttacgaaa ggtgctaggt aggctgtgcc taccacgttt
      241 ctaagaaaaa aatttgctat cactttttcg tcattgtgga tagcttatgt atagtgtttc
      301 ggcaaaaaac agctatacaa atactatatt tgtatgatat acataaatta tacacaaatg
      361 cgaaaggaat tcttctatgg acattttagt tcggaatatt gatccaaaag ttatttcaga
      421 aatagataga cgttgccaag aactcacaac aaaaactggc aaaaaatgga gccgtaatgc
      481 gtacctaaaa gttctaatcg aaaacgattt tgaccaggca cttaggcatt acaaaaaaga
      541 tcaattcgat ttattactcg aaaaatttat cgatatacaa gcaaaaaata ctgaaacatt
      601 gaacgactat gttcatacaa acaaccaact cattgaaaca cttatcggag gaatataaca
      661 tgagaaaaac ttctgttcaa aaacacgtta ccctttatcc cagtcacatt gacgttttag
      721 attccatcat cgaaaaaaat ccagatatca ataactactc ggaagccatt cgttttctat
      781 gtatgagtta tgaacctaat actaatacga caactaccag caaccaagca aaactaaatg
      841 cgatgggaaa agaaatttct attatgtccg aaatcattgc agaatttgct gatttatatc
      901 taaaagatac tggtgttctt atcggtacaa attctaaagt atatcaagaa gtgaaagaaa
      961 aaattgaaaa tcaactcaat ccctcccgac gaaatcaacc caaaaatcgt caaaaagaaa
     1021 ccaatcaaac attcacttct agaaattttg acccaacaaa ctttctaaaa agatgaaaaa
     1081 gaaaccgaaa attttttcta tttctcagtt tcttttttag ggggcagtct tccagcaaga
     1141 tttgcaagtt accggtaact tgcacaaaaa aagaaaaatc cgcttgtgcg aatccttctt
     1201 tttgcatttt ggggcgctgc cccaatctcc aaatatcggt tatttaatag cgtggtaact
     1261 caaaaaaata gaacgtactc tgaaataaaa aaagactgct ctaacgagca gccaataaaa
     1321 aataatgagc gtttattctt gaaaaagcag cgcgcttatg aagcgctgcc aaaaatttac
     1381 catattttat gaaaaataac aagtttctaa aaaggaatcg gttaagattc acaaagaatc
     1441 gtaaacagac aatagcggaa aatcaagctt ccgaacgtac cttttagcta gaccaatgaa
     1501 aaatcacaaa gtgagcggct ggatcggttg gtacaggggc tacttggcat agtcgacatt
     1561 atgtctacta gctcgataaa taacgattat gttcccaaaa caatatttta tgaactataa
     1621 caaaaatttg ttatcgcata ttgtatattt taattatagg tgtatatcac gtgaactact
     1681 caccacttag ctaaacctga cggttcttaa cgcttgaagt gggagcttct tgggaataga
     1741 gcatacttga tagcgtattt ctttactaac agcggtgtct cttatttacc aagctatccc
     1801 cgtagttcct acggttcttg atgattggat ctaagccaat cccatagaat gtagtcttag
     1861 accttcggtc agaatattga tactagcatt gatatcacga tcatgatgag tatgacaaac
     1921 aggacaagtc cattctcgaa tttcaagaga tttcttgcca tctttatgtc cgcactctga
     1981 acaaatctga ctagacggaa accatttatc tactttgatg atttttcgtc cataccagtc
     2041 agccttgtat tgtaatttag tcacaaactt tgaccaagaa acatcagaaa tacttttagc
     2101 taatttatga tttcgcaaca taccttttgt gtttaagtct tcaatacaga taatatcgtg
     2161 atttttgatc atttctgtac tcaacttatt cagaaaatca gtacgttgat tcattacttt
     2221 ttcatgcaat ctagctactt tgcgtttttg tttttgataa tttttagctt caaatagatt
     2281 ggtaccttta tttttagcca ataaagcacg cctagacaat ttgcgttgtt cacgctttag
     2341 tttcttttcc attttggacg tgaatttatt attatcaatt ttttgtccat cagaaagaat
     2401 cgcaaagtcc ataataccta agtcaatacc aattgtggaa ttagttttag gtaattcgat
     2461 aatttcttct ttacacaaca aagaaatata gtatttaccg ctagaatgac gtgatattgt
     2521 agcagatttg ataatccctt ttggttgtct atgaagctta attcttacta atgacttcaa
     2581 tttaggaact ttgatgaatt tactatcaat caaagcaact gtaccatttt gattattcgt
     2641 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
     2701 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
     2761 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
     2821 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
     2881 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
     2941 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
     3001 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgccaccag
     3061 ttgtcagaag acaaaaactt ttagaccaga acatttcttt ccaaagaaat tgtttgactc
     3121 ttggaaagtc acgttttatc aatcttgagc tggcactttt ataggcattg atgaattttg
     3181 tcaattctgt tttaggttga gctttgaaca taatatgaac gtggtcttta tcatgattcc
     3241 actccactaa agtaatgtga tacgattctg aaattctttc aaaagtagtt ttggcataat
     3301 cagatatttc atcatcaatc acttgtctac gatatttcgt tactaaaaca aggtggtaat
     3361 aaagaagaaa tactgaatga ttatttgtat ctaattccat gttatatcaa ctcatttctt
     3421 gtatagactg attgtaacat ggaacaaaat aaaaaggcaa tacgccttag ttgtcggtct
     3481 tcgagttacc aacggtaacc ctcataccga acggcattca tcacccacct atagaggatg
     3541 ggcgacttct gcctaattct gttaaattta acacaaaggc gtgattattt tgaaacccaa
     3601 aaagaaaatt tatctaggtc tcctcttttc cttgatgatt ggaattttta gtataggaat
     3661 tacaaatacc tatgcttcag aaaattctaa tttcaatatc aactcccaac caattgaaga
     3721 acaagcaatt aattttgcga aaactgattt tgaacactcc ataacttcaa ttcttttaat
     3781 ggaaaataac accgaaactg atattaacta tactcttggt tcgccattta aaatgctcga
     3841 tggagaaaat acggcaaata gttctatttt ctttccagta ttttcagacg ctgataatac
     3901 gattaaatat gtttataccg tcaaaaaaaa tacaaatgga gaatattcag gaagtatttc
     3961 acaattttta gcaaaagaac ttcaaaatct agtagatagt aatataacag atacaacctt
     4021 attttttcaa gataatagca tttattattc cgataatgga caaattaaac tactttggaa
     4081 atcaccttta agtacagaaa aaaatggtgt ttcttccctt acagaaaaaa atgatcttat
     4141 atccgtaaat ataaaaaata aaattaaaaa tttttctaaa aaacaaaatt ttcctgcggt
     4201 taaacgtgta aaaagtgtct cctccggaga taactttatt atgattaatt ggcgtatagc
     4261 agaaacccaa acaagttttc cgtggtgcgc tgcttacgta gaagctgcta tcttatgtaa
     4321 caaaacagat gtacgtgcta cttcagctag agccattatg cattatacct atcctaattt
     4381 aaacaacagt caaattgaat caaaaagtct taatcaagat caaataataa aatatgcaaa
     4441 ttactgtggt gtttatccta aaaaaatagc acgaatacta aacctttcag aggtccaaac
     4501 acaactaaga aaaggaaatg ccatttatat gggaactgct ggttatggaa attatagtaa
     4561 cagccgtcat gcacttgcat tatttgggtg ggtcaatcaa ggtggttatc aaacttacta
     4621 tgtgtggaat ccttggtata actacccaac tgtaataagt gcaaatgcag gaccagttac
     4681 aattcctgta cctggaggtg gttttacttg ggacaatacc attacaaatt ggtaaataaa
     4741 ctcattgctt tagttatggg tctagtgtta gttcttttct taactagttg ttcaaaaaaa
     4801 caagaaatac aaaagactga tctgattcct tttctaggaa cttggcaaac taaagatcat
     4861 tcagaacgtc tagtaatcac taatttaaat acttcaaaaa atatagaaca atataccatc
     4921 ttagtaaaag agaacaatca taaaattcaa atcattgata aaaatttaaa tataaatgaa
     4981 caagttttca tttacaaagc aacaaataat ttactaacac tgcgctcgaa taaaaaacaa
     5041 ttagattaca cttttagttt ggattctcca aaaaagctcg tatttatttt gacaacgaat
     5101 gatgggacag aagggacagc taaaccaatt gaatttttta aagctgattg atgaatagtt
     5161 gtttgatatt acaaaacaat aatttcaccg aaggtgaaac aagcgaaact ttacaaaaag
     5221 tggctagcat ttgctagcct tttttgtatt gtggagcgtg ggttttgagg gggattcaag
     5281 ggggagcttg cgcccctttg acaagcggtt atttgcacca aatacgtagg tatttggcta
     5341 gcaaataacc gcttgtcaag ccctttagtg tgttttgtag aaagggacct tatagtcact
     5401 ttcacattac aaacgatacg tcctatggac gtatcaaaag ggtgggactt atgagcaaat
     5461 taaagcaaaa tttggctagc aatccttcta aaaaatacac ctattgatca gagtcaaaat
     5521 aaatcagttt tcgagtgagc gaatccaaat ttgcaactct gaagctttgc aaatgagtgt
     5581 gccggcttcc gtaaaagtct aggtaacatt agtcgatata tacatagggc tctaatttgc
     5641 gttttaaggc gttttaaacg ttctggtatg taattatacc aaagcgactt taaaacgcct
     5701 taaaacgaat tttagaggtt ctgtagaacg ttttagggtt gttagggctt gccctgaccg
     5761 cctgtaaggc gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg
     5821 tagatgagcg ctcagagctt ctaaaaggac tccttacgaa ctgtttttaa gcaaaggagc
     5881 ctttaggcga ctgcgcagtt taatgtgaga gcgtagcgat cacaccttta aagcgaccgt
     5941 agggagcaga ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa
     6001 aattttaggc gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa
     6061 acctctttac cgtcggctgt atccttactc tcccaaggtg tgcagaatct tttaggggtc
     6121 tttttgtctg tttttagggg tctttttgtc tgtttttagg ggtctttttg tctgttttta
     6181 ggggtctttt tgtctgtttt taggggtctt taattagggg gttgaccccc attagaaagc
     6241 tctttataat tttgttaagt agttaatctt gaaggaggat tttatggcaa atgaaattgt
     6301 gaaataccac catgaattaa atacgattcc tttacgcaaa tttacgtcag tagaaatgaa
     6361 tttattcttc tctataatat ctagaatgcg tgatgtaggc gataaaaaag tacaatttac
     6421 attcgaacaa ttaaaagatt taagcaatta taaagcaact gcaaatgttc gattcataga
     6481 tgatttagaa acaacgtatg acaaattaat ggatttacgt tttggtagaa gaagtgctga
     6541 cggtttgcaa cgtgaacgat ttgttttatt taatcaattt aaaatcgatg gaaaagctga
     6601 tattcctttt gctgaaatac aagtccatga gaaagcctta cccttactta ataatcttga
     6661 agaatgggtt cgatattctc tccaacaatt caatgaatta gaaagcagtt attccaaaac
     6721 catgtttaga ttattaaaac aatacagaac caaaggaatt gccaattttt caaaagaaga
     6781 ctttcatgaa ttacttgata tccctaaaag ttatagagaa agtgatataa ataaaaaaat
     6841 tataaagcct attcgccaag aactaaccgc aatcttcaaa ggtttgacaa tccaaaagag
     6901 atacggaaaa ggacggggaa agcctgtcat tggctatcaa tttacattta aacctgagat
     6961 aaaaaaatca gacgacttta acaaacatgc tccaaccaaa gaagaacaaa ataaagcttt
     7021 ggaactagct aatcaacagg ttcaatctga aaaagaacct acttcattac aagaaaaaat
     7081 caaagaacaa aaacgaaaat tagaactttt acaacagtta gcagaattag aaagaaaaca
     7141 aaatacccca agtaagaaaa ttaacaaaca aatagcagat aacaattccg atattacccc
     7201 agaatttaaa gaaaacttac tagataatct ccaaaactta tttaaagaat aaggggtttt
     7261 tatgacagat aaaactatca aagaattagc agatattcta ggtgtttcaa aagataaagt
     7321 gaagtatcaa ctgaggaaat tacctagtaa ttttacctac aaaaaaggaa atattaccta
     7381 cctgaaaaaa gaagcagtaa caacaattac aaaggtttta tcaggtaatg aatcgggtaa
     7441 cttacctagt gaattacccg attcattacc taccgattac ctactcaagg agattgaaac
     7501 taagaataat catattgaag aaaaagatag gcaaattgaa aaacttcaaa tagctttaga
     7561 aacgcagcaa aaattactcg accagcaaca acaactcacc ctacaagcca atcaacaaat
     7621 caaaaaactt cacgaacaac tccagctaac ctatgcagaa gaatcttca
//
