LOCUS       NZ_CP083923            21133 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Enterococcus faecium isolate V1164 plasmid pV1164-3, complete
            sequence.
ACCESSION   NZ_CP083923
VERSION     NZ_CP083923.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA5367917
            Assembly: GCF_020162175.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21133)
  AUTHORS   Mollerup,S., Elmeskov,C., Gumpert,H., Pinholt,M., Sejersen,T.S.,
            Pedersen,M.S., Worning,P., Frees,D. and Westh,H.
  TITLE     Rapid in vivo development of resistance to daptomycin in
            vancomycin-resistant Enterococcus faecium due to genomic
            rearrangements
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21133)
  AUTHORS   Mollerup,S., Elmeskov,C., Gumpert,H., Pinholt,M., Sejersen,T.S.,
            Pedersen,M.S., Worning,P., Frees,D. and Westh,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2021) Department of Clinical Microbiology,
            Copenhagen University Hospital Hvidovre, Kettegaard Alle 30,
            Hvidovre 2650, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-JAN-2019
            Assembly Method                   :: Unicycler v. 4.0.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 254.19410228420895x
            Sequencing Technology :: Illumina MiSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 01:58:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,998
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 45 of 127
            Pseudo Genes (incomplete)         :: 76 of 127
            Pseudo Genes (internal stop)      :: 35 of 127
            Pseudo Genes (multiple problems)  :: 26 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083923.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..21133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="V1164"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pV1164-3"
                     /geo_loc_name="Denmark"
                     /collection_date="2015"
     gene            join(18965..21133,1..252)
                     /gene="pheT"
                     /locus_tag="LCG90_RS14720"
                     /old_locus_tag="LCG90_14745"
     CDS             join(18965..21133,1..252)
                     /gene="pheT"
                     /locus_tag="LCG90_RS14720"
                     /old_locus_tag="LCG90_14745"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /GO_component="GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA]"
                     /GO_function="GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="WP_002296881.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLKK
                     IVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAGNV
                     KIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDMDDS
                     IIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVEDKEAV
                     PAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFDYDKLK
                     SKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEITNETTTV
                     ALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSLAGGEVLQ
                     GAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEYVYSVVVPP
                     RRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRTIFEGSGLSE
                     AISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDISYNTARKNTEI
                     ALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDFFTIKGLLENLF
                     EQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITKSYDIPETYVAEF
                     DLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIRSAAGRFLTDIQLF
                     DVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALVENLEASIR"
                     /name="pheT"
     gene            complement(422..1432)
                     /gene="trpS"
                     /locus_tag="LCG90_RS14725"
                     /old_locus_tag="LCG90_14750"
     CDS             complement(422..1432)
                     /gene="trpS"
                     /locus_tag="LCG90_RS14725"
                     /old_locus_tag="LCG90_14750"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="WP_002288045.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIADM
                     QALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVSVG
                     RVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQTQE
                     IVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQKKVM
                     SMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIKRYLID
                     VLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINYFH"
                     /name="trpS"
     gene            1686..2423
                     /locus_tag="LCG90_RS14730"
                     /old_locus_tag="LCG90_14755"
     CDS             1686..2423
                     /locus_tag="LCG90_RS14730"
                     /old_locus_tag="LCG90_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="WP_002296877.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKSTM
                     LRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKLAP
                     IKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEMLLFD
                     EPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLEDSRN
                     VRDFFENPNEVRAQQFISKVINH"
                     /name="amino acid ABC transporter ATP-binding protein"
     gene            2437..3252
                     /locus_tag="LCG90_RS14735"
                     /old_locus_tag="LCG90_14760"
     CDS             2437..3252
                     /locus_tag="LCG90_RS14735"
                     /old_locus_tag="LCG90_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_002288041.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEIIW
                     GVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKNGN
                     IDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKGSTS
                     AANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVGGTFT
                     NEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
                     /name="transporter substrate-binding domain-containing
                     protein"
     gene            3265..3912
                     /locus_tag="LCG90_RS14740"
                     /old_locus_tag="LCG90_14765"
     CDS             3265..3912
                     /locus_tag="LCG90_RS14740"
                     /old_locus_tag="LCG90_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="WP_002288038.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSKN
                     KVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAETV
                     RSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSILAIV
                     AGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
                     /name="amino acid ABC transporter permease"
     gene            3928..4584
                     /locus_tag="LCG90_RS14745"
                     /old_locus_tag="LCG90_14770"
     CDS             3928..4584
                     /locus_tag="LCG90_RS14745"
                     /old_locus_tag="LCG90_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="WP_002296876.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIRF
                     ANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAMLS
                     EIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDTSLA
                     VIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY"
                     /name="amino acid ABC transporter permease"
     gene            4746..5567
                     /gene="racE"
                     /locus_tag="LCG90_RS14750"
                     /old_locus_tag="LCG90_14775"
     CDS             4746..5567
                     /gene="racE"
                     /locus_tag="LCG90_RS14750"
                     /old_locus_tag="LCG90_14775"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /GO_function="GO:0008881 - glutamate racemase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="WP_002317207.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARCP
                     YGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILPGT
                     RAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEYHSS
                     VAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGEVSML
                     LDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
                     /name="racE"
     gene            5570..6925
                     /gene="rph"
                     /locus_tag="LCG90_RS14755"
                     /old_locus_tag="LCG90_14780"
     CDS             5570..6925
                     /gene="rph"
                     /locus_tag="LCG90_RS14755"
                     /old_locus_tag="LCG90_14780"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /GO_function="GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0008033 - tRNA processing [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="WP_002296875.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIEN
                     SVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVVDL
                     EKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLAAVS
                     VGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEMLFFG
                     KNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGYHVKTL
                     LDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGIYSARFA
                     GEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGRVARIPSG
                     ENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGER"
                     /name="rph"
     gene            6926..7444
                     /locus_tag="LCG90_RS14760"
                     /old_locus_tag="LCG90_14785"
     CDS             6926..7444
                     /locus_tag="LCG90_RS14760"
                     /old_locus_tag="LCG90_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="WP_002288030.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPLW
                     DTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADMVF
                     FGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQAVEE
                     LAFSFSRER"
                     /name="metallophosphoesterase"
     gene            7551..8051
                     /gene="cbpB"
                     /locus_tag="LCG90_RS14765"
                     /old_locus_tag="LCG90_14790"
     CDS             7551..8051
                     /gene="cbpB"
                     /locus_tag="LCG90_RS14765"
                     /old_locus_tag="LCG90_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /GO_function="GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="WP_002295789.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVGY
                     SKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWELE
                     EVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKEQID
                     IVS"
                     /name="cbpB"
     gene            complement(8386..9681)
                     /locus_tag="LCG90_RS14770"
                     /old_locus_tag="LCG90_14795"
     CDS             complement(8386..9681)
                     /locus_tag="LCG90_RS14770"
                     /old_locus_tag="LCG90_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="WP_002297218.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRAC
                     RSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSY
                     FVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKIL
                     PRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFL
                     VTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKL
                     KKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHEL
                     VEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
                     /name="ISL3-like element ISEfa11 family transposase"
     gene            9952..13479
                     /locus_tag="LCG90_RS14775"
                     /old_locus_tag="LCG90_14800"
     CDS             9952..13479
                     /locus_tag="LCG90_RS14775"
                     /old_locus_tag="LCG90_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="WP_002296887.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKFE
                     QEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILRQI
                     LEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQLKMK
                     DLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQLLQA
                     MMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRFAPEQT
                     RPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRRLVSEEN
                     YRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLFALDRYSY
                     RLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQGTYWTREKD
                     WQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEAANVFYQFLI
                     QSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESAFDWSLFQEII
                     TSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDRKETKGLLSSEE
                     REVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQSYDTDGSLKMSPY
                     LRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLALDEKKPVPKHWQKL
                     KELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTSISRMENFYNCEYKY
                     FMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLVNMSDAQRRDFTEKVL
                     QEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNTGMHPLQTEILFGQIAG
                     AKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDYKSSGRSFDVTEAYYGMA
                     MQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTETDDVEKNTLKKFSYDGVFV
                     DDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKDKFFTEEELQLFMAHNRENM
                     RLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDVMLKENNYHRLEKIDKEEALR
                     RILKRSEEE"
                     /name="PD-(D/E)XK nuclease family protein"
     gene            13476..17198
                     /gene="addA"
                     /locus_tag="LCG90_RS14780"
                     /old_locus_tag="LCG90_14805"
     CDS             13476..17198
                     /gene="addA"
                     /locus_tag="LCG90_RS14780"
                     /old_locus_tag="LCG90_14805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]; GO:0016818 - hydrolase activity, acting on acid
                     anhydrides, in phosphorus-containing anhydrides [Evidence
                     IEA]"
                     /GO_process="GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="WP_224471342.1"
                     /translation="MNQIPLKPKNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVRR
                     VIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVLLP
                     TANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAENDER
                     FFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSRLYQK
                     QIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDRLEEAY
                     AGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQMEELTDKA
                     LPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPSEASKHYR
                     KKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLADPSLFIGK
                     YENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAAKLIQGFSDF
                     PENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLIDSSFMIYDKK
                     SKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQATEIRTMISLLQL
                     IDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQASNEDELAAKLEH
                     FGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQANLYALVDRAKAYEQ
                     SSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKGLEFPVVFLLDMTKE
                     FNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQNKLLSEEMRKLYVGL
                     TRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGRSSLMNWIGYGLIRHPE
                     MQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKELVNLDQQMKEDETLLAD
                     SLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESRLTLESSQNKAASRQFRYT
                     QEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTESIHQKLQELVRKRLVDEKV
                     AKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLPADEVFQDYPNQEDELLIHGI
                     VDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQGQLRLYQQAMSEALNKPVENV
                     FLILLRVKQIININK"
                     /name="addA"
     gene            17267..17608
                     /locus_tag="LCG90_RS14785"
                     /old_locus_tag="LCG90_14810"
     CDS             17267..17608
                     /locus_tag="LCG90_RS14785"
                     /old_locus_tag="LCG90_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002303789.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVELA
                     AKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVWSE
                     KWITEEECG"
                     /name="helix-turn-helix domain-containing protein"
     gene            17917..18960
                     /gene="pheS"
                     /locus_tag="LCG90_RS14790"
                     /old_locus_tag="LCG90_14815"
     CDS             17917..18960
                     /gene="pheS"
                     /locus_tag="LCG90_RS14790"
                     /old_locus_tag="LCG90_14815"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /GO_component="GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA]"
                     /GO_function="GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="WP_002295783.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEVL
                     RGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGRKV
                     KHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDTFYI
                     SDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEGLVVD
                     KNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGCNVCKY
                     TGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFYQNDLRF
                     LSQFKGE"
                     /name="pheS"
ORIGIN
        1 ttgcttgtaa aagaaactgt gacaaatcag gaattaacag aagttatccg ttctgctgcc
       61 ggaagatttt taacagatat ccaattattt gatgtctatc aaggagaaaa cattgaaaaa
      121 ggacataaat ccatggcgta tagcctgaca ttcgtcaacc cagaagcaac attgacagat
      181 gaagaaatca ataaagcaat ggaaaaagtc gaaaaagcgt tggtagaaaa cctagaggca
      241 agtattcgct aagaaggaaa atatagtaag taacgatcaa gtagcttttg aagaccatac
      301 atttgaaaaa aagatgtgac acaacttcgt cacatctctt tttttgattt taaaaaagga
      361 atagcacaga gacgagataa aaaaacaggc ctttacagcc tgctttttta gagtaaatga
      421 attaatggaa gtaattgatt cccattgctg attttacttc agaaagtgtt tgtgcagcta
      481 ctttagcagc ttcttcacta cctttacgta agatttccat aacagctgaa ggatcttttt
      541 ccaattcttc tcggcgcgca cggatcggtg caaattcagc ttccaataca tcaatgaggt
      601 atcgtttgat ctttacatca ccaagtccgc cgcggcggta atgagctttc atttcttcaa
      661 ttgcttcttt atctgttccg aatacatcca agtaggtgaa gaccatattc ccttctactt
      721 gtccaggatc ttgtacatgg atgtggtttg gatcagtata catactcatg acttttttct
      781 gtaagacatc ggcaggatcg gagatataaa taccgtttcc taacgattta ctcattttgc
      841 catttccatc gattccaggt aatctaccca ttccttttgg tgggaatacg ccttttggtt
      901 ccaccaagac ttcaccatat gtatgattga agctttgtac gatttcctgt gtctgctcta
      961 gcattggttt ttgatcctca ccaacaggta caagattcgc tttgaatgca gtgatatctg
     1021 ctgcttgaga aacaggatag ataaagaatc ctgtaggcac gctttcaccg aatttttttt
     1081 gttcgatttc tgttttgaca gtcggattgc gccgaacgcg tcctacgctg actaagttca
     1141 aataatacat ggttaattca gctaattccg gaatttgtga ttggataaat aaagtagatt
     1201 ttgcaggatc taatcccacg gctaaatagt ctaaagcaac ttgcaagaca tttgatgaaa
     1261 ccttctcagg atttttagca ttatctgtca gtgcttgcat gtcagcaatc atgacaaaca
     1321 gttggttgtc ttcatctgcc tgcatagcca ctcgggtttt tagagaaccg acgtaatgcc
     1381 ctaaatgcag cttcccagtt ggtcgatctc ccgttaaaat aatatttttc aaaaaagaca
     1441 cctctttacg atttgtaagt ctgagcactt aatatcttac cgtttttcct gaaaagaaac
     1501 aacagttcct aaggaaaaaa caaggaaaga ggataaaata actaatagat tagaaatgga
     1561 agaaatattt atgggagaat aaacgttcta agaaacttta caaattacta aactatcaga
     1621 tataatgtat tttattatac atctttaaaa gaaatcttac taaataataa tgggagtgta
     1681 attctatggc aatgattaaa tttgatcacg tggataaata ttatggcaag ttccacgcat
     1741 taaaaaatat caatctcgaa ttcgagaaag gagaagttgt cgttgtcatt ggtccttctg
     1801 gttcaggaaa gagcacgatg cttcgctgta tcaatggatt agagacgatc tcttcaggta
     1861 agctgctgat taatgatact gacttgcatg ataaaaagac aaaactgaca gaagtccgaa
     1921 aaaacatcgg catggttttc caacatttca atttgtaccc aaataaaact gttctggaaa
     1981 acatcaaatt agcaccaatc aaagtgttga agcaagacca ggcaactgca gtcaaaaacg
     2041 cggagaaatt tttgaaaact gtcaatatgt tagataagaa agattcttat ccttccatgt
     2101 tatccggggg acaacagcaa cgggtggcaa tcgccagagg tttagcgatg aatcctgaga
     2161 tgctgctgtt tgatgaacct acttctgctt tggatccgga aatgattggt gatgtattag
     2221 acgttatgaa aaaattggct catgacggta tgtcaatgat tgtcgtgaca catgaaatgg
     2281 gctttgccaa agaagtggcg gatcgtgtta tcttcatggc tgatggagaa gtattggaag
     2341 acagcagaaa tgtgagagac tttttcgaaa atccaaatga agtccgagca cagcaattta
     2401 tcagtaaagt tatcaatcat tgataggagg aaaaagatgc gcaggttaaa aaaaatacgc
     2461 ctgattttcc ttttaggcat tgcttttctt gcactagccg gatgtaaagg aaatgggttg
     2521 gcaaatgaag atattttaga gcgaagcaaa tctaccaatg aaatcatctg gggtgtcaag
     2581 tatgatactc gattatttgg aatgatggat attgagagca gaactgtaca aggatttgat
     2641 gtagatattg caaaagcaat cactaaaaag atccttggtg acaatggaaa aacagaattt
     2701 gttgaagtca cttctaaaac tcggattcca ttattgaaaa acggaaatat cgatgcaatt
     2761 attgcaacga tgacgatcac agatgaacga aaaaaacagg ttgacttttc agatgtctat
     2821 ttcgatgctg gccaagcttt gttggtaaag aaaggaagcc aaatcaaaag tgtagatgat
     2881 ttgaatgctt ctacaacggt tctagcagtc aaaggttcta catcggctgc gaatatccga
     2941 cagcatgcac cagatgcaaa aattttagaa ttagaaaact atgcagaagc atttactgct
     3001 ttacaatctg gtcagggaga cgcgatgact acggataatg cgattttgtt agggattgca
     3061 gatgaaaatc cagagtatga gttagttggt ggaacgttta ctaatgagcc ttacgggatt
     3121 gcgatcaaca aagggcaaga aaacttcttg aaagctgtta accaagcgct agaggaaatg
     3181 catgcagatg gcacatacga taaaatctat caaaaatggt ttccaaatga aactgaagga
     3241 aaagtagagt aagggggaga ggcaatgagc gaattgatac aaacttatgg accaatcttt
     3301 gtcgacgggt tcaagatcac attgttttcg agtgtcttag cacttgtgtt tagtttgatt
     3361 atcggaacgt taatggcaat cttccaatta tcaaagaata aagtagtgag cgccttagca
     3421 aaggcatatg tagaattttt tagaaacatt ccattattaa tcatcgtgat gtttttttat
     3481 gttgtcgtac cgatttattg gtttaatcta gatggattcc aagcaggtac gattggatta
     3541 acgatctata catctgcttt tattgctgaa acagtacgtt cagggattca aaccgtacca
     3601 aaaggtcaaa cagaagcagg actatcttct ggttttacct attcagaaat catgcgttat
     3661 attgttttac cgcaagcatt caaaatcgtt atcccgccat taggcaacca atttatcaac
     3721 ctcgtaaaaa actcatctat tttagcaatc gtagctggtt tagatttgat gtatcaagga
     3781 gacttgatcg ctagtgaaac attcaatacc ttcgatacgt atatcatcgt tgggttattt
     3841 tacttagtta tcacgctacc gttatcttac ttaatggttt atcttgagaa aaaatgggcg
     3901 gttcgttcat agaaggaggg gaaatacatg gattttagcg gcgctttttc atggatgaat
     3961 atccgttttt tattggaagg attaaaggtt actgttgaag tttcattgtt ttcgatcatt
     4021 tttagtttct tgatcggtgg tctgacaggt gtgattcgtt ttgcgaatat ccctttcttt
     4081 tccaaaatcc ttggtgtagt gattgatatt atacggaatc ttccattatt attgatcatt
     4141 ttcttcactt actttgcctt acctcaaatc ggcattcaga tgaacatctt ctggtccgct
     4201 gtcgcagcat taacgatttt tgagtcagct atgttgtcgg aaattttccg agctggactc
     4261 aatgctgttc caaaaggaca gatggaagca ggattatcaa cgggactgac ttatgtagaa
     4321 tcgatgcgaa cgatcgtgtt accgcaagca tttaaatcaa tgatcccggc aattgtgagc
     4381 caattgattt cactgatcaa agatacttca ttagcagtaa tcatttcttt acctgaactt
     4441 tctcatcagg cacgtattat ttatggacag aacaccaatt atgtgcttcc gatgttcatc
     4501 atcatgacac ttatgtactt cgtggtatgt tacgcgcttt ctctgctttc cggttacttg
     4561 gaaaagagac ggtacagtta ttaatagatg attcttttag cggttgtggc aaactaaagt
     4621 cacaaccgct tttcctcttt ccattcgaaa aaaagagatt tcgattgttt taagcaagta
     4681 agagcacttg aaaattcacg ctaaaaagta cacaataggg aggatagaat ttattgaaaa
     4741 aaaggatgat acgattgaca gataatcgcc ctatcggatt tattgattca ggtgtcggcg
     4801 gcttgactgt agtaaaagaa gccctgaaac aattaccgaa tgaaaatatt ttatttgtag
     4861 gagacacagc acgctgccca tatggcccta gacccgcgga acaggtaatc cagtatactt
     4921 gggaaatgac ggattatctg gtggagcaag gaatcaagat gctggtgatc gcctgcaata
     4981 ccgcaactgc ggtggcttta gaagaaatca aagctgctct ttctattcca gtcatcggtg
     5041 tgatccttcc cggtactaga gcggcagtaa aaaaaacaca aaataaacaa gttggcatta
     5101 tcggtacgat tggtacggta aaaagtcaag cttatgaaaa agcactgaaa gagaaagtac
     5161 cagaattgac tgtgacaagt cttgcttgtc ccaaatttgt ttcagttgtc gaaagtaatg
     5221 aataccattc atcggtggcg aaaaaaattg tggcagaaac attagctcct ttaaccacta
     5281 aaaaaatcga tacattgatt ttgggatgca cccattatcc attattacgc cccatcattc
     5341 aaaatgtaat gggagaaaat gttcaactga tcgattctgg agcagaaaca gtaggtgaag
     5401 tatctatgct gttagattat ttcaatttga gcaattcacc gcaaaatggt cggacattat
     5461 gccagtttta tacaactggc tctgccaaac ttttcgagga aatagctgaa gactggcttg
     5521 gaatcggaca cttaaatgta gaacatatcg aattgggagg aaaataagga tgcgtcatga
     5581 cgggagaaaa gcccaagagc ttcgcaaact ggagcttcaa acaaatgttt tcaaatatcc
     5641 agaaggatcg gtagtgatcc gttttggtga tacaacagtt atttgttctg caacaataga
     5701 aaattctgtt cccccttttt tgagagaaac aggaacagga tgggtgacag cagaatatag
     5761 tatgctgcct cgagcaacga gtacgagaaa tcgtagagaa agcagcaaag ggaaactttc
     5821 tggtagaaca atggaaatcc agcgtcttat tggtcgttcc ttgcgtgcag tagtagactt
     5881 ggaaaaatta ggagaacgta gtatcatcgt agactgcgat gtgatccaag cggatggcgg
     5941 tactagaaca gcaagtatca ctggggcatt tgtggcattg aaattagcca ttgaaaaact
     6001 tttaagagaa aaagaattgt cagaagatcc aataaaagaa catcttgctg cagttagcgt
     6061 aggaatctta ccagatggga cttgcgtcac agatttggat taccaagaag attccgcagc
     6121 acttgtagac atgaatcttg taatgacaga atctggaaaa ttcgtggaga ttcaaggaac
     6181 tggagaagaa gcaacgtttg atggtgaaca gttgaatgaa atgctctttt ttggaaaaaa
     6241 cgcgattgaa gatttgatca aagagcaaaa acatgctttg ctaacagagt ttgctcaaaa
     6301 tgatgagcgg atagaagaaa caaaaacaat catcattgcg acaagaaatc caggaaaagc
     6361 agaagagttt cgaaatatgt tcaaagaagc gggatatcat gtaaaaacat tacttgacta
     6421 tcctgaattg ccagatgtgg aagaaactgg cagcacattt gaagaaaatg cccgattaaa
     6481 agcagaaacg attgcacaat tgcttgatca gccagtctta gcagatgatt caggtttgaa
     6541 agtggatgct ttaggcggta tgccaggaat ctattctgca cgttttgcag gggaacaaaa
     6601 aagtgatgcg gggaataacg ccaaattatt atatgaattg acagatgtgc cggatgaaaa
     6661 aagaacagca caattccatt gtacgttagt attcgcggca cccaaaaaag acagccttgt
     6721 agtagaagcg gaatggcccg gtcgagtagc ccgtatacca tctggcgaaa atggctttgg
     6781 gtatgatcct ttatttatcc cagaaggtaa gaaacaaaca gcagcagaat tatcgagcga
     6841 agaaaaaaac aagatcagtc atcgggctca agcaatgaaa aaattgagtg ccgagtggaa
     6901 acaatggctg gaaggagaga gatagatgcg gtatttagta gtaagtgata atcatggaga
     6961 cagagatatc ctcgttgact tagtagatcg ttatcgttct gaagtggata tctttttcca
     7021 ttgtggtgat tcggaattag aagcttctga cccattatgg gatacattcc aagtggttca
     7081 aggtaattgt gattatggac caggattcga acagaaaaaa gtgatccaaa ctggtcagga
     7141 tacagtattc atgactcacg gtcatttatc gaatgtccga tttggtttga cacagttagc
     7201 aatcgaagct cgagctgcaa ctgccgatat ggtgtttttt ggtcataccc atcaaatagg
     7261 ctgtgagatg gatcagaaaa tcctgttttt gaatcctggt agcatttctc agcctagagg
     7321 cccgatccaa atcccatcat atgctcttat cgaaagttta gatgaccaaa tttctgttca
     7381 atactataac cgtgcccatc aggcagttga agagttggcc ttttcttttt cgagagagcg
     7441 ctgaataaaa aaactctaat ttttaataaa aataagcttt attttatgaa ttttgcatcc
     7501 ggtttcggta aaatagtagt gttgaatgaa atagaaatgg aggccatcag atgattggac
     7561 ctatagtaag agaaatgctg ctggaaaatc aggaaacgtt tttgattcct gcagaaaatg
     7621 tggcaaatgt tatgtatcat catccattgt cccatggttt acttgtgtta tcgaaggtcg
     7681 gttattccaa aattcctgta ttaggaaaag atgatcactt tgtaggacta gtaagtttat
     7741 cagatgtggt caataaaatg attgacttgc aaacaatcag catggacccg ttagaaggac
     7801 taacggttgc tgatgtgaca gaaacagatg ttccgacaat tgatgaaaat tgggaactag
     7861 aagaagtact ccatttgtta gtcgattcgc cttttttacc ggttgtcaca gaagatcgga
     7921 ttttcaaagg aatcattaca agaaaagagc tgcttaaagc cgtaaacttc atggtgcatg
     7981 aattagaacg tcgtaatata ttgtctccga aaatcgaaga attaaaagaa caaatcgata
     8041 tcgtgagcta aagaacgaga aactaaaaga taatcaaaaa ataagtagaa aaaacagcgt
     8101 accagttttg actttatctt gaaaaagagg ctgggacaaa agcgtttaac tccaagaaat
     8161 aagaaggaat tcacgaaaat tgcttttcaa atttttgtga atttcagctt atttccgaag
     8221 gagttgcttc tgcttccgcc gtttatacgt gtttagagcg tgggacaaaa gtgttcttta
     8281 cttttgtccc acgctctttt tagggctctt tgtcaataag gactgatgag ttgtgcaaaa
     8341 tcaaatctga gtcagaatga accacattct ggctcagatt ttttgttatc ttacattgat
     8401 taattgattg atcaaaaaga ttctaattct catgttctca aatgatttaa atccgtagga
     8461 tactcgtttc attgtcttta tgtgggtatt cttcgcttct atttttccat tggaataagg
     8521 atagatcatt gcgttggtga tgccttcttc ataggtcaga aggttttgaa gcttttcccg
     8581 aaagctgtca tctaacgttt cgggaagttc tgccaataag gagaaaaata agtcagggtc
     8641 tttgtctcga aaggcttcaa ctaattcatg aaaaaaggga tacgcctcct ttaggggcgg
     8701 agaaaactca agcaatcgat caatcatcat tgcttcagta agaaatgggt attttggtgc
     8761 tcggaaactt ttccatgttt tgtattcata atggttgatg tttgcacgat tttttagcaa
     8821 aaagcgccag ttctttttca gtttttctgc ctggcttttc tgtccggctt tacgaagttc
     8881 attcatttca cggatacgca actcattgaa cgcttgattc atgtgtttga caatatgaaa
     8941 ccgatcaatc acgactttcg catttggcag aacacgtttg gtgagctgga agtaggcggc
     9001 gttcatgtct gtcaccaaga attctacttc ttctggattg gtacaaccta agaaatagct
     9061 tgttaatcga ggtaatttac gcgtaggcaa aacatctatt aattttcctg tttcgccatc
     9121 cgcgcaaata aagctcatct tatcttctat ggaagcatgc gaacgaaatt cgtcaaccat
     9181 caatactctg gggaggatct tcttagattg ctttggtaaa tagcttttaa actctttcaa
     9241 tgtacgaata acggtggtca aagatacctg acagcttttc gcaataaaag ataaagatac
     9301 tttttcagtc agtaaagaag caattttata tctaacatga tttgcgattg aatgtctggg
     9361 ttggacaaaa taactttgag ccgtccaatg ggtgcgacag tttttacagg tatagcgttg
     9421 cttttttagg cgcataacca aaggcatatg attgtattgt tcaaaacgga caatcgtttc
     9481 cttttttcca tttttcacta taattgcttt cccgtttcca tctaccacag tagaaccaca
     9541 acttctacaa gcacgaggag taggcgagag aacagcatcg acgaccaacg tctttttctt
     9601 ctgaagggtc tcgtaagaga cctctgtaat catcaaatct ttctctgtta ttctcagcat
     9661 ttttttgata gaatcattca tatagcgtat cgtcctctca gttgtttatt ttgtggtgat
     9721 ttaatcatac tagagaacga tatgtttttt aatacctaaa atgaaaatgg ggctgaagaa
     9781 tcaattctga ttcatcagtc ccataaatta tagagccgtt ttttgcgtat acttaaaaaa
     9841 gtagaaatat cagaacctta aaaggatata atttgaagaa tatagaacaa atgttcagta
     9901 ttttttgata taataaagga gcaccgtatc atgtgttgaa ggaggaaaag catgtcttta
     9961 caatttattc ttggaaacgg gaaaaaagat caccgtcaag tcttgatcga cgaagcatcc
    10021 gcttggctcg accaggatgc aagtaaccaa gtatttttcc ttgttcccaa ctacagcaaa
    10081 ttcgaacagg aacaagaaat tttagcggag atgcgaagaa gaagcggaaa aaaaactttc
    10141 agcacaacaa acgttcaagt atttagtttt tatcgtctgg cttggtattt gctgcaacag
    10201 acaactcttc ttacaggaaa tgaattatca gaatcaggat ctgcaatgat cttgcgacag
    10261 attttagaaa aaaatgagga agaattaaca atctttcgcg gagaaatcaa taaaagaggg
    10321 tttattcgtc aattacaaga actctacagt gaattgcgta caggaaatat tcaaccagaa
    10381 gacctgatgc ttttatttgg tgattcacct aatgcagaag accagcaact taaaatgaaa
    10441 gacttgaagc tgattttttc agcttatgac agcgaattga ctcaacgtgc actaacaaat
    10501 gaagatgcac tatctatact agcaaattat atgcagacac aagatttcaa caacgtcaaa
    10561 tttattgtca gtggtttttc tcgaatcaat ggtcaagagt atcaattact gcaggcaatg
    10621 atggaaaagg ggcaattggt tgtcgattta ttgctggatc gtccttatac agcggatctt
    10681 ccagaagtac ttacgctctt tcatgagact ggaaagcttt atttccgtct ttttcaaagt
    10741 gcaagagaac ggaaagtccc tatattagtg gaccgatttg caccagagca gactcgtccg
    10801 acagagcttc aactgctgca aacaatgtgg gaagagacat ctagccaaaa gaaagcggca
    10861 gtacacaaaa tagggggaca agagcatctc catttgtggg aagctgaaaa tccaactgaa
    10921 gaagtccgac aattagctgt tgaaatcaga cgactagtca gtgaagaaaa ctaccgttat
    10981 cgtgatatcc aagtgctgac aaaaaatatg tctttatatg gaaatatcct tcggccgatc
    11041 tttcaagaaa tggcaattcc tttttatatc gatgaagaac aaatgatgga aaatcatccg
    11101 ctgatcgaat ggatcaacag tctgttcgct cttgaccgct attcttatcg gctaagtgat
    11161 attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg aagcagaaac cgaacttgat
    11221 gaatggaaac aagagcgaga tgaattcagg aaaaaactgg acatcactga aaatgtagca
    11281 cttgcttaca attatcaagg aacttattgg actcgtgaaa aagactggca gtttgtggcg
    11341 tatgattttg aagcagaaca gattgaagac gtacagtatt tggaagaaca gtcaaatacg
    11401 attagacgat tatttcagaa agctgtacca gtatttttga aaaaaataaa gaaaagcaaa
    11461 acaggacaag aggccgcaaa tgttttttat caatttttga tccaaagtgg cgtacagcaa
    11521 caattattgt tttggcgtaa tcaggaagta gacagaggaa acttagaagc ggcaagaaac
    11581 catgagcaga catggggagc gctgattgat ctgttggatg aatatgtagt aatttatggt
    11641 gaatcggctt ttgactggtc actgttccaa gaaatcatta caagtggtct agaaaatcta
    11701 agttatggta aaatccctac cgcaatcgac caagtgcgaa tcaatcgttt ggaattagtt
    11761 cgaccaaatc aagctgccgt cacttttgca ttagggttga atgatcaagt gtttccagac
    11821 cgaaaagaga caaaaggatt gttgtccagt gaagaacgag aagtattaaa tcaggccttg
    11881 ccagatggac agtttttatt cgacccatca aaggaaagta tctcttttga gcctttccaa
    11941 gcctatcttg tttttctttc cggaacagat cgattatatc ttagttatgc acaaagctat
    12001 gacacagatg gttcgctgaa gatgtctcct tatttgcgta gaatcacaga atatttatct
    12061 gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg accctaattg ttatgtaggg
    12121 acttacagaa gcggaatcaa tacagtcaat aggatttatc gattagcttt agatgaaaaa
    12181 aaacctgtac caaagcactg gcaaaagctc aaagaattag tactgaattc gtcatggcga
    12241 aattttgctt tacagatttt tgaaagtcag gaacatttaa atgttcctgt ttcgatgcca
    12301 gaaaaaatgg cggaagagct ttatggaaaa gagatttata cttcaatttc acgtatggag
    12361 aatttttata attgtgaata caaatacttc atgcaatttg gtttgaaatt gaaagaacga
    12421 actgtttatg gtctaactcc agcagcaact ggtgattttt atcatgaatc attggatcgt
    12481 ttcttcaagc tactgttttc aaaccaatta tccttagtga atatgtccga tgcgcagcgt
    12541 agagacttta ctgaaaaagt gctgcaggaa gtattcggag aacttcggtt tgagatattg
    12601 gatagttctg caagaatgaa ctatatccgc taccagttag gacagacgat tcaaaaagtg
    12661 acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc atcctcttca gacagagatt
    12721 ctatttggtc agattgctgg agcaaaaggt atcacaggtc tggaattacc gttgagtaat
    12781 ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg atgtagcttc tgaacaagaa
    12841 gatacttggc taagcgtcgt tgattacaaa tctagcggac gttcattcga tgtcacagaa
    12901 gcttattatg gcatggctat gcaattattg acgtatctgg acgtagcatt gatggatgcg
    12961 gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat catattattt acatgttcat
    13021 aatcctgtat tgacggagac agatgatgta gagaagaata cattgaaaaa atttagttat
    13081 gatggcgttt ttgtcgatga tcccgctctt ttagaagtac tagatcattc actggaagca
    13141 aaagaacaat cactgatcta tccagtgaaa aaaaatgcga aagatgaata ccaaaaaggt
    13201 agtttcagta aagacaaatt tttcacggaa gaagaactgc aactattcat ggcgcataat
    13261 cgggaaaata tgcggttagc tggagatgcg attacttccg gagaaatcaa actaaatcca
    13321 gcttataaag ataaacagcg gattgcctgc gaattttgtc cattcagaag catctgtacg
    13381 tttgatgtga tgctgaaaga aaataattac caccgtctgg agaaaataga taaggaagaa
    13441 gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa tcaaattcct ttaaaaccga
    13501 aaaatgaacg attcacagat gatcagtggc aagcgatttt tgatcaagga gataatctat
    13561 tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct tgttcgaaga gtgattgaaa
    13621 aactaaaaat gggttttgat atcgatgaat tattgattgt gacatttact gaagctgccg
    13681 caagagaaat gaaagaaaga attcaagagg cattacaaga atccgtcaat agcgaaagtg
    13741 accctgtacg tagacagcat tttactaagc agttagtttt attgcccaca gcgaatatct
    13801 caaccttaca tgctttttgc ctgacagtta ttcgacgtta ttattactta attgatattg
    13861 atccagtctt tcgcatgtta acagatgaaa cagaaacgat cttgatgaag gaggatgtgt
    13921 gggacgagct gagagaagca ctttatgcag aaaatgatga acgatttttc caattaacaa
    13981 tgaatttttc taatgatcgt tcagatgacg gcttgacgaa tctggtgttt tctctttatg
    14041 agtttgcccg tgccaaccct gacccccaaa aatggctaga acagttaagc gataattatc
    14101 gtttgcctga ggggctagcg aaatctcgat tatatcaaaa acaaatccgt cctttagttt
    14161 tggcagatat ataccaatgt gtacagttat atgaacagat gactcagtta gcacaaggtg
    14221 aaggattaga aaaaatgcat gagcaagtag ctggagaaca acagcagatc aagaatatct
    14281 atgaagcgtt ctcgcaagat cggttagaag aagcttatgc tggtttggaa caactaactt
    14341 tttcaacttt taagagcagt cggaaagcag aattgaaaga aatttccaat gaagtcaagg
    14401 gcatgaggga taaagcaaaa aaactgattc agcagataag taaaagttat tttcctgttt
    14461 ccccaagcca aatggaagaa ttaacggaca aagcactacc gttagtcgaa gaaatgacaa
    14521 aagtcacgca aagcttcatg gacggtttca gtatgcgtaa acgggaaaaa ggtgtgctgg
    14581 atttcaatga tctggaacat ttagcgttgc agattctcac agaaaaaacg aaagatgtat
    14641 ggctgccaag tgaagcttca aaacattatc gaaaaaaatt caaagaagta atggtagatg
    14701 aatatcaaga cgtgaaccaa ttacaagaag ccattttata ctggctaaga gaacccgatg
    14761 atacaaaagg aaatatgttc atggttgggg atgtcaaaca atccatctat tctttccgtt
    14821 tagcagatcc tagtttattt atcgggaaat acgagaattt ctcaaagaaa gaaggcggac
    14881 gtcgaatcgt tttggctgag aacttccgct ctagaaaaga agtcctttct tttacgaatc
    14941 ttatttttga acaactaatg gatccagcag tgggtcaaat caattatgac gaagcagcca
    15001 aactgatcca aggattttca gactttccag agaatgaaca gtttgagccg gaaatcatga
    15061 tctatgaaaa agaacaggaa gaatcagaga ttgagattcc gacggatgat atactggaag
    15121 acaaaacaga aggcgaactt tttatgacag gactaaaaat tcgtcaatta attgattcct
    15181 cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc gatagagtac aaagacattg
    15241 ttctcttgac acctacaaag aagaacaact taacgatttt agagattttc aaaacattgg
    15301 atattccatt agagatgaat gatgctcaaa actattttca agcgacagaa attcgcacga
    15361 tgatctcgct gctacaatta atcgataatc cctatcaaga tattccgctt gcggctgtat
    15421 tgcgctcacc gatcgtgggt ctgattgaac cagaattagc cagcattcga ttagctaacc
    15481 gtgcacatac ttactatgat gctgtgcttg cttatcaagc atcaaacgaa gatgaattag
    15541 cagctaagct ggaacatttt ggcaagcaac tagagcattg gcgagaactt gctcgccgtt
    15601 cttcgatcac tgacttgtta tgggatatct attatgagac gggatatcta gagtatgtgg
    15661 tcggtctgcc agcaggggca cagagacaag ccaatcttta tgcattggtt gatcgtgcca
    15721 aagcttacga gcaaagttct ttcagaggat tataccagtt cgtgcgtttc attgaaaaaa
    15781 tgcaagaaaa agataaagac ttagcagaac cagtcatttc aatagaagat aatgctgttc
    15841 gcgttatgac gatccatgca agtaaaggct tggaattccc agttgtattt ttgttagata
    15901 tgacaaagga gttcaactta caagacttgc gcaatcggta tgcttttgaa gaaaaacttg
    15961 gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt atatgatacg ttaccgtttc
    16021 aagccatcaa attggcaaag caaaacaaat tgttgtcgga agaaatgcgt aaattgtatg
    16081 taggattgac tcgggcagaa caaaagcttt tcattgtcgg ttcctataaa aataaagagc
    16141 aaatgatcca aacgtggtct gaagcagctg atcatgagga gttagttttt gatcctgcgc
    16201 tacgtttgaa gggcagaagc agcttgatga attggattgg ttacggattg atccgtcatc
    16261 ctgaaatgca gaaatactta gaggaagaaa tttcgacttc tcttcttcaa cattcaaatg
    16321 cacagttttc tatttcatgg atgaatcagc aatcgatcat tgaacaaaga cagttgcttg
    16381 ctgaaaaaga actggttaac ttagatcagc agatgaaaga agatgaaact ttattagcag
    16441 attcgttaca aaaacgtctg gcatatgaat atccttatca agcttcttca caaaccacaa
    16501 gttatcaatc tgtatctgaa atcaaacgat tattcgaaga tccagatgat acacaagaat
    16561 cacgtttgac tctggaaagc agtcagaaca aagcagcatc ccgtcaattt cgttatacgc
    16621 aagaacaact agcagaacca aaatttttgc aaaaagatcg gcaagtgtca gcagctgctg
    16681 ttggaactgc tacgcatgct ttgttacaat tactgccatt agagatgcca actacagaga
    16741 gtatccatca aaaattacag gaattggtaa ggaaacggtt agtagacgaa aaagtcgcga
    16801 aaaaagtaga tgtttcttct attatatggt tctttcaaac agaacttggt caacagctga
    16861 ttgccaacaa agaaaacgta aaacgagaac aacctttttc catgctgctt cctgctgacg
    16921 aagtatttca agactatcca aatcaagaag atgaactatt aatccacgga atcgtcgatg
    16981 gatacctaga agaaaaagat catctcaata tctatgattt taaaacagat tttattttac
    17041 atccggatga tccagcagaa atagatgcaa tcgtccaaaa atatcaagga cagctaagac
    17101 tttaccaaca agcaatgtca gaagctttga ataagccagt agaaaatgtt ttcctaattt
    17161 tgcttcgagt gaaacaaatt ataaacatta acaaataatt ataattgagc aaacttgact
    17221 tatgttttgt aaggattatg ctatactctg ggaagaggtg agtatgatgg agctaacaca
    17281 gacagaattc gttttatgcc ctaaatttga aaaaacattc tctattttag gaaaaaaatg
    17341 gaacggattg attatcgatg tactactcga aaacggtccg caacgctttg ttgagcttgc
    17401 ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa cgcttgaagg aattagaaaa
    17461 agaaggaata gtcactcgaa actttgcctg tagcgaaaag agccgttcag aatatctctt
    17521 gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa atacaagttt ggagcgaaaa
    17581 atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa aatcttgtaa actatagtcc
    17641 atacgaataa gtaaagaacg atgacgaaaa agagtagttt ggctaaacca gttcagggag
    17701 atctgtcgca gactgaaagc agatcctgtg tttgaaaaat gaagttcact ttccgagttc
    17761 gtctgataaa atcagaccgg caattgtcgt taacgaagta agagtgcggt gtaagtcatt
    17821 tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct taacaggaat gaggtctttt
    17881 tgttttgtat ttggaattta aaggaggaac aagcacatgt ctctacaagc acaattagag
    17941 agcttgaaag aagcaacgct cgtcaaaatc acagcggcac aagatttaaa tgaattaaat
    18001 caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca cagaagtatt acgtgggatg
    18061 aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt tcgcaaatga gatccgagat
    18121 cttttgacag aagagatcga gcgaaaaaaa caagtattag aagaagaagc gttaaatgca
    18181 gctttggcaa aagaaacaat agacgtgaca ctacctggta gaaaagtaaa acatggtaca
    18241 cgtcatatcg tgacacaaat catggaagaa attgaagata tttttgtggg tatgggctat
    18301 cagatcgtag aaggatatga agtagaatct gatcattaca atttcgaacg gatgaatctg
    18361 ccaaaagatc atccagcaag ggacatgcaa gatactttct atatttcaga cgagatattg
    18421 attcggacac atacttcacc agtccaagca cggacaatgg aaaaacatga tttctctaag
    18481 ggtgctctac gaatgatctc gccagggaaa gtcttccgca gagatacaga tgatgcgacc
    18541 cacagtcatc agttccatca aattgaaggg ctagttgttg ataaaaacat cacaatgggc
    18601 gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt ttggggaaga tcgtgaaatt
    18661 cgtttgcgtc caagttattt cccatttacg gaaccatcag tagaagtaga tgtcagctgt
    18721 ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca ctggatggat cgaaatttta
    18781 ggagctggca tggtacaccc gaatgtgttg aagatgtcag gaatcgatcc agaagaatat
    18841 tcaggctttg ctttcggcct tggaccagac cgcgtagcta tgttgcggta tggagtgaat
    18901 gatatccgta atttctatca aaatgactta cgtttcttaa gtcagttcaa gggggagtaa
    18961 gaaaatgtta gtttcatata aatggctcaa tcgttatgta gatttatcga atgtaacacc
    19021 aaaagaatta gcggataaga tgtctgtgac aggaatcgaa gtagagggag taacggttcc
    19081 agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag gaatgcgtac cacatccaga
    19141 ttctgatcac ctttctattt gtcaagtcga tattggcgaa gacgaactgt ctcaaatcgt
    19201 ttgtggagcg cctaatgtga aggttggaat caaagtgatt gttgcacttc ctaactcacg
    19261 tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt ggccaagtat caaatggcat
    19321 gatttgctca ttacaagaaa ttggctattc agatagtgtt ataccgaaag aatacgctga
    19381 aggtatctat tatatgccac aagaagctgt aaatggcgat ccagtttttc cttatttgga
    19441 tatggatgat tcgattattg aattatctat cacaccgaat cgtgcggatg cattgtcaat
    19501 gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag actcctacat tcgatgacga
    19561 aaagctggta gaatcctcac gtcctgctag cgataaaatt caagtggaag ttgaagataa
    19621 agaagcagtt cctgcttatc aaatcagaat cattgaagga gtaagaatcc agccaagtcc
    19681 tcaatggctg caaaatcttt tgatgaacga aggcattcgt ccaatcaaca atgtagtaga
    19741 tgtgacgaac tatattttgc ttttattcgg acaaccacta catgcatttg actacgacaa
    19801 attgaaatca aaagagatct ttgtccgccg tgcaaaagaa aatgaaacat tagtaacact
    19861 agatggagaa tctcgggaat tgacaactga caatctcgtc attactaacg gcaaagaacc
    19921 agttgcttta gctggtgtca tgggagggtt agactcagag atcacgaatg aaacaacaac
    19981 agttgcacta gaatctgctt tatttgatcc aatttcgatt cgtcgtacag caaaacaatt
    20041 taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga atcaaccatg cgactgttgg
    20101 agaagccagt gaggttgctg cagcaatgat cgtttctctt gcgggtgggg aagttctaca
    20161 aggcgcggtc aaaggctcag agatcaaagc agaagacgta tcagtgaaga tttcacttga
    20221 acgcatcaat caatatttag gaacatctct tgtcgttaaa gaagtaaatg agatttttga
    20281 agcacttgga tttagctatg aagaaaagga gtatgtttat tccgtagtag taccaccaag
    20341 acgctgggat atccaaatcg aagcagatat tatcgaagaa gttgctcgta tttatggata
    20401 cgaccagttg ccatctacac ttccaagtgg agaaacggtt gctggaagct tgtcaaaaga
    20461 acaagaaact actcgtaaaa ttcgtacaat ctttgaagga agcggattga gcgaagcaat
    20521 cagttatgca ttgacaacag aagaaaaatc aagacagttt gttataaatg aaagtaatat
    20581 tacacgtttg gactggccaa tgagcgaaga acgttctgta ttacgaatga atttgatcag
    20641 cggactatta gatgatatca gttataacac tgcccgaaaa aatacagaga ttgctttata
    20701 tgaaattggt cgggtgttct tccaagaaaa tgatccacgt acacatctgc cgcaagaagt
    20761 aaaacatgca gcaatcgcta tttctggtat ttgggaagaa aaagattggc agacgaaagc
    20821 tcaaaatgta gactttttca ccatcaaggg tcttttagaa aacttatttg aacaactagg
    20881 catcactgaa gatgtccatt atcaaacagt gacagaaatg aaggaaatgc atcctggaag
    20941 aactgctgcg atttaccttg gcgaaaaatt tattggtttt gtcggtcaag tgcatccaac
    21001 gattacaaaa agttatgata ttccagaaac atatgttgct gagtttgatt tatctgctat
    21061 agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg acaaaattcc cagcagtcag
    21121 ccgggacatc gct
//
