LOCUS       NZ_CP083896             7250 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Escherichia coli strain NDM1 plasmid unnamed8, complete sequence.
ACCESSION   NZ_CP083896
VERSION     NZ_CP083896.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21398202
            Assembly: GCF_020149645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Simner,P., Mostafa,H., Bergman,Y., Ante,M., Tekle,T., Adebayo,A.,
            Beisken,S., Dzintars,K. and Tamma,P.D.
  TITLE     Progressive Development of Cefiderocol Resistance in Escherichia
            coli During Therapy Is Associated with Increased blaNDM-5 Copy
            Number and Gene Expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Simner,P., Mostafa,H., Bergman,Y., Ante,M., Tekle,T., Adebayo,A.,
            Beisken,S., Dzintars,K. and Tamma,P.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) BioIT, Ares-Genetics, Karl-Farkas-Gasse 18,
            Vienna 1180, Austria
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Canu v. JAN-2021
            Assembly Name                     :: Isolate1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 67x
            Sequencing Technology :: Oxford Nanopore MiniION + Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 01:54:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,150
            CDSs (total)                      :: 5,024
            Genes (coding)                    :: 4,771
            CDSs (with protein)               :: 4,771
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 1 of 253
            Pseudo Genes (frameshifted)       :: 124 of 253
            Pseudo Genes (incomplete)         :: 145 of 253
            Pseudo Genes (internal stop)      :: 42 of 253
            Pseudo Genes (multiple problems)  :: 55 of 253
            Pseudo Genes (short protein)      :: 4 of 253
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083896.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NDM1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: Baltimore"
                     /lat_lon="39.296 N 76.591 W"
                     /collection_date="2020"
                     /collected_by="Johns Hopkins University"
                     /genotype="ST90"
     gene            complement(join(7090..7250,1..190))
                     /locus_tag="LCE25_RS25905"
                     /old_locus_tag="LCE25_25545"
     CDS             complement(join(7090..7250,1..190))
                     /locus_tag="LCE25_RS25905"
                     /old_locus_tag="LCE25_25545"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_224446649.1"
                     /translation="MLNDEFRKLEESVNRELTSNESLIRNAINDHTTALKELLERYQKT
                     TVDTMDALENRTENERETRLRPDTPKVNGLTRPGGSLSAPGIRSQTSCDRLRELHVSAV
                     FTVIPEACEATA"
                     /name="MbeB family mobilization protein"
     gene            complement(<129..946)
                     /locus_tag="LCE25_RS25550"
                     /old_locus_tag="LCE25_25550"
                     /pseudo=""
     CDS             complement(<129..946)
                     /locus_tag="LCE25_RS25550"
                     /old_locus_tag="LCE25_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(936..1259)
                     /locus_tag="LCE25_RS25555"
                     /old_locus_tag="LCE25_25555"
     CDS             complement(936..1259)
                     /locus_tag="LCE25_RS25555"
                     /old_locus_tag="LCE25_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1322..1513
                     /locus_tag="LCE25_RS25560"
                     /old_locus_tag="LCE25_25560"
     CDS             1322..1513
                     /locus_tag="LCE25_RS25560"
                     /old_locus_tag="LCE25_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1536..1937)
                     /locus_tag="LCE25_RS25565"
                     /old_locus_tag="LCE25_25565"
     CDS             complement(1536..1937)
                     /locus_tag="LCE25_RS25565"
                     /old_locus_tag="LCE25_25565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224446647.1"
                     /translation="MENASGAAFPGVSFFAHMFFPGLSPGSVDNRVTVFEKGESARRSR
                     TTERSESVSEEAEERLNWHFLLTHLCGISHRTWRTFHTIRTDAEWLSQYTLRYRYVTGS
                     GLRPDTPKVNGLTRPGGLVCSRHPLTDKL"
                     /name="hypothetical protein"
     gene            complement(2712..3440)
                     /locus_tag="LCE25_RS25570"
                     /old_locus_tag="LCE25_25570"
     CDS             complement(2712..3440)
                     /locus_tag="LCE25_RS25570"
                     /old_locus_tag="LCE25_25570"
                     /inference="COORDINATES: protein motif:HMM:NF013088.3"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_096956412.1"
                     /translation="MDSSFYKKKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAG
                     SASIIFSLESVFILVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVG
                     YCLVVAGVLSAAFYVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLES
                     VIIVFSSGILQYFLAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQG
                     VGFIVTVLSVYFLNRKYSEE"
                     /name="DMT family transporter"
     gene            complement(3401..3577)
                     /locus_tag="LCE25_RS25690"
     CDS             complement(3401..3577)
                     /locus_tag="LCE25_RS25690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.3"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="WP_249540724.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKNSH"
                     /name="EamA family transporter"
     gene            complement(3579..4631)
                     /locus_tag="LCE25_RS25580"
                     /old_locus_tag="LCE25_25580"
     CDS             complement(3579..4631)
                     /locus_tag="LCE25_RS25580"
                     /old_locus_tag="LCE25_25580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(5112..5345)
                     /locus_tag="LCE25_RS25585"
                     /old_locus_tag="LCE25_25585"
     CDS             complement(5112..5345)
                     /locus_tag="LCE25_RS25585"
                     /old_locus_tag="LCE25_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5352..5882)
                     /locus_tag="LCE25_RS25590"
                     /old_locus_tag="LCE25_25590"
     CDS             complement(5352..5882)
                     /locus_tag="LCE25_RS25590"
                     /old_locus_tag="LCE25_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(5949..6554)
                     /locus_tag="LCE25_RS25595"
                     /old_locus_tag="LCE25_25595"
     CDS             complement(5949..6554)
                     /locus_tag="LCE25_RS25595"
                     /old_locus_tag="LCE25_25595"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_224446645.1"
                     /translation="MPGGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFA
                     KKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVI
                     PNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQ
                     ALAEGVTRGIDALYHAGEIKGRQDVIQALTEAGLKWSG"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6560..6883)
                     /locus_tag="LCE25_RS25600"
                     /old_locus_tag="LCE25_25600"
     CDS             complement(6560..6883)
                     /locus_tag="LCE25_RS25600"
                     /old_locus_tag="LCE25_25600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6946..7044
                     /locus_tag="LCE25_RS25910"
     CDS             6946..7044
                     /locus_tag="LCE25_RS25910"
                     /inference="COORDINATES: protein motif:HMM:NF013937.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_318011531.1"
                     /translation="MTKQEKTALNMARFIQRPDINAAGEAQRTGRG"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(1608..1689)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(2142..2329)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(191..690)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.407"
                     /coverage="100.0"
                     /evalue="2.3300000000000003e-132"
     Typing          complement(940..1292)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.458"
                     /coverage="100.0"
                     /evalue="3.22e-69"
     Typing          complement(5009..5970)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6160..6524)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.18"
                     /coverage="99.0"
                     /evalue="2.15e-75"
     Typing          complement(6564..6916)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.458"
                     /coverage="100.0"
                     /evalue="3.22e-69"
ORIGIN
        1 gcgtttcacg ctcattttca gtacggtttt ccagtgcgtc catcgtatcg acggtcgttt
       61 tctgatacct ctccagcagt tctttcaggg ctgttgtatg gtcgttgata gcgttcctga
      121 ttaaagattc gtttgaggtc agttccctgt ttacagattc ttcgagcttc ctgaactcgt
      181 cgttcagcat tttctctgta gattcggctc tctttcagct ttttctcgaa ctccgcgccc
      241 gtctgtaaaa gattgctcat aaaacgctcc tttcagtctg atattcctcc cgccgttcgg
      301 gtctgcaatg ctgatactgc ttcgcgtcac cctgaccact tcaagccccg cctctgtgag
      361 cgcctgaatc acatcctgac ggccttttat ctctccggca tggtaaagcg cgtctatccc
      421 gcgtgtgacg ccctcagcaa gcgcctgttt cgtttccggc aggttatcag gaagggtcag
      481 cgtccggcgg ttctccgggg cgttggggtc atgcagaccg taatggtgat tcaccagcgt
      541 ctgccaggca tcaattctcg gcctgtctgc ccggtcgtag tacggctgga ggcgttttcc
      601 ggtctgtagc tccatgttcg gaatgacaaa attcagctca agccgtccct tgtcctggtg
      661 ctccacccac aggatgctgt actgattttc tcgagaccgg gcatcagtac acgctcaaag
      721 ctcgccatca ctttttcacg ccctccaggc ggcagctcct tctccgcgaa cgacagaaca
      781 cctgacgtgt atttcttcgc aaatggcgtg gcatcgatga gttcccggac ctcttccgga
      841 ttccctgaag caccgttgcc ccttcccggt tacgctccct tcccagcagg taatcaaccg
      901 gaccactgcc accacctttt cccctggcat gaaacttaac tatcatcccg cgccccctgt
      961 tccctgaccg ccagacgcag ccggcgcagc tcatccccga tggccatcag tgcggccacc
     1021 acctgaaccc ggtcaccgga agaccactgc ccgctgttca ccttcctcgc tgtctggtta
     1081 aggttattcc cgatggcggc cagctgacgc agtaacggcg gtgccagtgt cggcagcttc
     1141 ccggaacggg caaccggctc acccaggcag acccgccgca tccataccgc cagttgttta
     1201 ccctcacagc gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc
     1261 ctctcgcgtt tcatcggtat cattaccccc atgaacagaa cccccttaca cggaggcatc
     1321 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc ttcatcagag gccagacatt
     1381 aacgctgctg gagaagctca acgaactgga cgcggatgaa caggcagaca tctgtgaatc
     1441 gcttcacgac cacgccgatg agctttaccg cagttgcctc gcacgcttcg gggatgacgg
     1501 tgaaaaccgc tgacacatgc agctcccgga gacggtcaca gcttgtctgt gagcggatgc
     1561 cgggagcaga caagcccgcc agggcgcgtc aggccgttga cctttggggt gtcggggcga
     1621 agccctgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacca ttcggcatca
     1681 gtgcggattg tatgaaaagt gcgccatgtc cggtgtgaaa tgccgcacag atgcgtaagg
     1741 agaaaatgcc agttcaggcg ctcttccgct tcctcgctca ctgactcgct acgctcggtc
     1801 gttcggctgc ggcgagcgga ctctcctttc tcaaaaacgg taaccctgtt atccacagaa
     1861 cccggggata aaccaggaaa gaacatgtgc gcaaaaaacg aaaccccggg aaaggccgcg
     1921 ccggaggcgt tttccatagg ctccgccccc tgacggactt cacaaaacag acactttttc
     1981 ggtgcatctg tgggagcctt gcggctcaac catgaaatcc gccagcggtg gcgaaacccg
     2041 acgtggctta aagatcatca ccgtttccgg tgaatcgctc cctcctgcgc cccacgttcc
     2101 cgccatatcg attaccggat accttttccg cctttctccc ttacgggaca cgtggcgctt
     2161 tctcatagct cacgtcttct ggtatctcgg ggcgacttag gcagttcgct ccgggctggg
     2221 ctgtatgcag gaaccccccg ttcagcccga ctgctgcgcc ttctccggta actgttctct
     2281 tgggtccaac ccggaaaaga caccacaaag cgccactggc agcagccatt ggtaaaagtc
     2341 atgtgcgaga tatttagaga gtacttgaag tggctgccgg agtgcggcta cactagaaga
     2401 acaattttgg tgactcgtct cgcataagac agttacctgg ttaagcagtc tgcaagctga
     2461 cttaaccttc gatcaaaccg ccctgcaagg cggttttttc gtttacaggc agcagattac
     2521 gcgcagaaaa aaaggatctc aagaggatcc tttgatcttt ttgttacaaa cgtctctatt
     2581 tgactcccct gagagcgttt tagcgcgcct ctgaccccct tggttacatt ccgacaccag
     2641 aaacgctccc gttttttacg tgcatttggc acggtatcga tggaggccgg ggggcaatcc
     2701 tgaacagaat gttactcctc tgagtatttt ctgttaagaa aatatacact tagcacagtt
     2761 acaataaatc ctaccccctg taagaatgtg aattgttcat ccaaaaacaa ccatgacaaa
     2821 aataatgcaa ctagcggaat aaagtacaac attatccctg ccaaatgaac tgggagccaa
     2881 cgcaaggagt tcaagtaaaa acaaaaagct aaaaaatatt gtaatattcc agatgaaaaa
     2941 acaataatca cggattccaa cggaagataa attgatgatt tagatataaa aataaagagc
     3001 gatgacagta taagggagaa tgtcaactgt ccgctcaata atgtgatagg ctcaaacgat
     3061 ttaataaggc gagacgatat aacaacataa aatgctgccg ataacacacc ggcaacaaca
     3121 agacaatatc caacaatatt attcgatcca ttactgatgt ctggcaaggc taccaaaata
     3181 ctgccaacaa ttgcacctat aaggaacaat ataaaatatt gcggggaatt aattttaact
     3241 gagcaaataa gaacagacaa caccaatata aacactgatt caagtgaaaa aataattgat
     3301 gcgcttcctg ctgaaataaa ataaagtccg tagagtgtaa aagtatacgc taagaatggt
     3361 tcaaaaacgc ctgtcgcata agcaagtatc gcttttgagt ttaatgggag ttttttcttt
     3421 ttataaaaag aactatccaa gatagtatta ctgcactaca aatctgccag aaaaacaccc
     3481 ctccggcatc tagttcttca actccttttt tgctcattac gatagctgcc ccccatgaga
     3541 acgctgctaa aaaagcaaaa agcattcctt taatcatatt aatcatcctc tttctcataa
     3601 aggggtttgt tttcaaattt ccttggttga aaggagttaa aaaacataga tgcatcatct
     3661 acaggaatac caataaactc tccataataa ggatcatcta taggaaactc ttctaatgaa
     3721 acataaaaca atacattcca gttgaagatc attggatcac tattttctat ttcatggagc
     3781 aatctttcat ccttagttaa ttctcctgga attaatacat cagtatgata aaaaccgctg
     3841 taataagaac tattaccggt gtgcactggg catccaaaac gatccaaatt agattctgaa
     3901 tgccatgttg tagaaatccc ttccggatgt gaatacccac ccttaccaac agactgaata
     3961 gtcatgttac gaatatccgc taacgcttgt tcttgatctt tatagaaacc tttttctatc
     4021 gccagcctca taaaatgccc cggggcattt acagaaataa gtattccccc tggtattctt
     4081 ctatcatgat ttcttgttct atgagcagaa gagtaaaaaa cattaacacc agccttatat
     4141 atttttagcc catctttatc ctgaaatggt acgctttcag tataaataac atcctctttt
     4201 attggatagc actctggcat atgcagcgaa caaatatatt tttgtatgtc cactaactct
     4261 tctgatggac gagcaaaggc aagttgtgga tgaataaaat aaatgagatg cgcactgacc
     4321 tctcctcttc ttgcgcgttc tttaaaacga actctttcat tacgcaaaaa atcaaacaaa
     4381 tgaccatcat cattatatag ttgggggcta tttacaatag ataaatggaa atcaagccct
     4441 ttaattttct tgcttgccaa tttaccaaat acacagggct gattgcctga cacccatttt
     4501 cttagtaaat cttcaatttt atctttagaa gtttcgttaa agataaatga atgaatatta
     4561 tcaatatctt ttccaaatga aaaactggat gataattgtg gtagtttatt tatacgttgt
     4621 tgaagattca tgaaagctcc cacaagacac ctcatatatt tttttatata ctgaaactac
     4681 attatattta ttaccggcgt aaaacgaaat aatttattat cgttaccgat attttaaaaa
     4741 aatcattatt tgcacagtgt tcgagttgtt agtatcactc cttcctgaaa atccgctcca
     4801 tttaccataa cgtcgcattg tacgcaccgc caaaacgcgt tcggtgcgat cacggcagca
     4861 gacaggtaaa aatggcaaca aaccacccga aaaactgccg cgatcgcacc tgataaattt
     4921 taaccgcatg aatacctatg caaccagatg ctacaggcca cattagcccg ccttaatcca
     4981 ctgaacacgt catttttcat ggtttcacca tcccagcgaa gggccatcca gtgtccgctc
     5041 ctggatttcc ggctgacgct cccgctccag agataactca tgttcgcgtt cctgcatcag
     5101 ccgttcatcc cttatctcct gttctcgctg tataactggc tcaagcgttc tgtccgctcg
     5161 ctcaagtgct gcacctgctg actcaactgc atgactcgcc cgttcagcat cgcgttgtcc
     5221 cgttgcgtaa gcgaaaacat tttctgcaat tccacgaagg cgttctccca ttcgttcagc
     5281 cgctgcatat agtcctgttg cagttgctct aatgcgttca gcaaatgtct ttccagctcc
     5341 gtcatgctct gtcactcctg caccagtctc actgcgttca gtttgccgtt gtccttcgtc
     5401 cagttcgttt cggctttcat ccccttcggc aacaccagaa aacgtccgtt gctgtcctcg
     5461 tgataacgca cgccccatgt cttcgcgttc agcttctcca gactctcttt ctgctcgcgg
     5521 agaatattca tattcgcgtt tatcttcatg ccctggtacc acagcagact cccggtcgtg
     5581 gcaaacatca gtactgagac gataatcagc cacagccagc gtttcacgct cattttcagt
     5641 acggttttcc agtgcgcgtc catcgtatcg acggtcgttt tctgatacct ctccagcagt
     5701 tctttcaggg ctgttgtatg gtcgttgata gcgttcctga ttaaagattc gtttgaggtc
     5761 agttccctgt ttacagattc ttcgagcttc ctgaactcgt cgttcagcat tttctctgta
     5821 gattcggctc tctctttcag ctttttctcg aactccgcgc ccgtctgtaa aagattgctc
     5881 ataaaacgct cctttcagtc tgatattcct cccgccgttc gggtctgcaa tgctgatact
     5941 gcttcgcgtc accctgacca cttcaagccc gcctctgtga gcgcctgaat cacatcctga
     6001 cggcctttta tctctccggc atggtaaagc gcgtctatcc cgcgtgtgac gccctcagca
     6061 agcgcctgtt tcgtttccgg caggttatca ggaagggtca gcgtccggcg gttctccggg
     6121 gcgttggggt catgcagacc gtaatggtga ttcaccagcg tctgccaggc atcaattctc
     6181 ggcctgtctg cccggtcgta gtacggctgg aggcgttttc cggtctgtag ctccatgttc
     6241 ggaatgacaa aattcagctc aagccgtccc ttgtcctggt gctccaccca caggatgctg
     6301 tactgatttt tctcgagacc gggcatcagt acacgctcaa agctcgccat cactttttca
     6361 cgccctccag gcggcagctc cttctccgcg aacgacagaa cacctgacgt gtatttcttc
     6421 gcaaatggcg tggcatcgat gagttcccgg acctcttccg gattcccctg aagcaccgtt
     6481 gccccttccc ggttacgctc ccttcccagc aggtaatcaa ccggaccact gccaccacct
     6541 tttccccctg gcatgaaact taactatcat cccgcgcgcc ctgttccctg accgccagac
     6601 gcagccggcg cagctcatcc ccgatggcca tcagtgcggc caccacctga acccggtcac
     6661 cggaagacca ctgcccgctg ttcaccttcc tcgctgtctg gttaaggtta ttcccgatgg
     6721 cggccagctg acgcagtaac ggcggtgcca gtgtcggcag cttcccggaa cgggcaaccg
     6781 gctcacccag gcagacccgc cgcatccata ccgccagttg tttaccctca cagcgttcca
     6841 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctcg cgtttcatcg
     6901 gtatcattac ccccatgaac agaaccccct tacacggagg catcagtgac caaacaggaa
     6961 aaaaccgccc ttaacatggc ccgcttcatc cagaggccag acattaacgc tgctggagaa
     7021 gctcaacgaa ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
     7081 cgatgagctt tacgcagttg cctcgcacgc ttcggggatg acggtgaaaa ccgctgacac
     7141 atgcagctcc cggagacggt cacagcttgt ctgtgagcgg atgccgggag cagacaagct
     7201 tccgccaggg cgcgtcaggc cgttgacctt tggggtgtcg gggcgaagcc
//
