LOCUS       NZ_CP083895             6665 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Escherichia coli strain NDM1 plasmid unnamed7, complete sequence.
ACCESSION   NZ_CP083895
VERSION     NZ_CP083895.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21398202
            Assembly: GCF_020149645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Simner,P., Mostafa,H., Bergman,Y., Ante,M., Tekle,T., Adebayo,A.,
            Beisken,S., Dzintars,K. and Tamma,P.D.
  TITLE     Progressive Development of Cefiderocol Resistance in Escherichia
            coli During Therapy Is Associated with Increased blaNDM-5 Copy
            Number and Gene Expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Simner,P., Mostafa,H., Bergman,Y., Ante,M., Tekle,T., Adebayo,A.,
            Beisken,S., Dzintars,K. and Tamma,P.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) BioIT, Ares-Genetics, Karl-Farkas-Gasse 18,
            Vienna 1180, Austria
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Canu v. JAN-2021
            Assembly Name                     :: Isolate1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 67x
            Sequencing Technology :: Oxford Nanopore MiniION + Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 01:54:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,150
            CDSs (total)                      :: 5,024
            Genes (coding)                    :: 4,771
            CDSs (with protein)               :: 4,771
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 1 of 253
            Pseudo Genes (frameshifted)       :: 124 of 253
            Pseudo Genes (incomplete)         :: 145 of 253
            Pseudo Genes (internal stop)      :: 42 of 253
            Pseudo Genes (multiple problems)  :: 55 of 253
            Pseudo Genes (short protein)      :: 4 of 253
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083895.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NDM1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA: Baltimore"
                     /lat_lon="39.296 N 76.591 W"
                     /collection_date="2020"
                     /collected_by="Johns Hopkins University"
                     /genotype="ST90"
     gene            185..361
                     /locus_tag="LCE25_RS25675"
     CDS             185..361
                     /locus_tag="LCE25_RS25675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_263481672.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLH"
                     /name="DMT family transporter"
     gene            444..869
                     /locus_tag="LCE25_RS25680"
     CDS             444..869
                     /locus_tag="LCE25_RS25680"
                     /inference="COORDINATES: protein motif:HMM:NF013088.3"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_263481673.1"
                     /translation="MSAGSASIIFSLESVFILVLSVLICSVKINSPQYFILFLIGAIVG
                     SILVALPDISNGSNNIVGYCLVVAGVLSAAFYVVISSRLIKSFEPITLLSGQLTFSLIL
                     SSLFIFISKSSIYLPLESVIIVFSSGILQYFLAFVFT"
                     /name="DMT family transporter"
     gene            complement(2249..2440)
                     /locus_tag="LCE25_RS25510"
                     /old_locus_tag="LCE25_25510"
     CDS             complement(2249..2440)
                     /locus_tag="LCE25_RS25510"
                     /old_locus_tag="LCE25_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2505..2828
                     /locus_tag="LCE25_RS25515"
                     /old_locus_tag="LCE25_25515"
     CDS             2505..2828
                     /locus_tag="LCE25_RS25515"
                     /old_locus_tag="LCE25_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3037..>3468
                     /locus_tag="LCE25_RS25685"
                     /pseudo=""
     CDS             3037..>3468
                     /locus_tag="LCE25_RS25685"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3506..4036
                     /locus_tag="LCE25_RS25525"
                     /old_locus_tag="LCE25_25525"
     CDS             3506..4036
                     /locus_tag="LCE25_RS25525"
                     /old_locus_tag="LCE25_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            4043..4276
                     /locus_tag="LCE25_RS25530"
                     /old_locus_tag="LCE25_25530"
     CDS             4043..4276
                     /locus_tag="LCE25_RS25530"
                     /old_locus_tag="LCE25_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4756..5812
                     /locus_tag="LCE25_RS25900"
                     /pseudo=""
     CDS             4756..5812
                     /locus_tag="LCE25_RS25900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            5814..6500
                     /locus_tag="LCE25_RS25500"
                     /old_locus_tag="LCE25_25500"
     CDS             5814..6500
                     /locus_tag="LCE25_RS25500"
                     /old_locus_tag="LCE25_25500"
                     /inference="COORDINATES: protein motif:HMM:NF013088.3"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_263481671.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFVFT"
                     /name="DMT family transporter"
     Typing          2074..2155
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          1430..1617
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          2819..4347
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 gtgatccaat gatcttcaac tggaatgtat tgttttatgt ttcattagaa agagtttcct
       61 atagatgatc cttattatgg agagtttatt ggtattcctg tagatgatgc atctatgttt
      121 tttaactcct ttcaaccaag gaaatttgaa aacaaacccc tttatgagaa agaggatgat
      181 taatatgatt aaaggaatgc tttttgcttt tttagcagcg ttctcatggg gggcagctat
      241 cgtaatgagc aaaaaaggag ttgaagaact agatgccgga ggggtgtttt tctggcagat
      301 ttgtagtgca gtaatactat cttggatagt tctttttata aaaagaaaaa aactccatta
      361 aactcaaaag cgatacttgc ttatgcgaca ggcgttttga accattctta gcgtatactt
      421 ttacactcta cggactttat tttatttcag caggaagcgc atcaattatt ttttcacttg
      481 aatcagtgtt tatattggtg ttgtctgttc ttatttgctc agttaaaatt aattccccgc
      541 aatattttat attgttcctt ataggtgcaa ttgttggcag tattttggta gccttgccag
      601 acatcagtaa tggatcgaat aatattgttg gatattgtct tgttgttgcc ggtgtgttat
      661 cggcagcatt ttatgttgtt atatcgtctc gccttattaa atcgtttgag cctatcacat
      721 tattgagcgg acagttgaca ttctccctta tactgtcatc gctctttatt tttatatcta
      781 aatcatcaat ttatcttccg ttggaatccg tgattattgt tttttcatct ggaatattac
      841 aatatttttt agcttttgtt tttacttgaa ctccttgcgt tggctcccag ttcatttggc
      901 agggataatg ttgtacttta ttccgctagt tgcattattt ttgtcatggt tgtttttgga
      961 tgaacaattc acattcttac agggggtagg atttattgta actgtgctaa gtgtatattt
     1021 tcttaacaga aaatactcag aggagtaaca ttctgttcag gattgccccg gcctccatcg
     1081 ataccgtgcc aaatgcacgt aaaaaacggg agcgtttctg gtgtcggaat gtaaccaagg
     1141 gggtcagagg cgcgctaaaa cgctctcagg ggagtcaaat agagacgttt gtaacaaaaa
     1201 gatcaaagga tcctcttgag atcctttttt tctgcgcgta atctgctgcc tgtaaacgaa
     1261 aaaaccgcct tgcagggcgg tttgatcgaa ggttaagtca gcttgcagac tgcttaacca
     1321 ggtaactgtc ttatgcgaga cgagtcacca aaattgttct tctagtgtag ccgcactccg
     1381 gcagccactt caagtactct ctaaatatct cgcacatgac ttttaccaat ggctgctgcc
     1441 agtggcgctt tgtggtgtct tttccgggtt ggacccaaga gaacagttac cggagaaggc
     1501 gcagcagtcg ggctgaacgg ggggttcctg catacagccc agcccggagc gaactgccta
     1561 agtcgccccg agataccaga agacgtgagc tatgagaaag cgccacgtgt cccgtaaggg
     1621 agaaaggcgg aaaaggtatc cggtaatcga tatggcggga acgtgggggc gcaggaggga
     1681 gcgattcacc ggaaacggtg atgatcttta agccacgtcg ggtttcgcca ccgctggcgg
     1741 atttcatggt tgagccgcaa ggctcccaca gatgcaccga aaaagtgtct gttttgtgaa
     1801 gtccgtcagg ggggcggagc ctatggaaaa agcgcctccg gcgcggcctt tcccggggtt
     1861 tcgttttttg cgcacatgtt ctttcctggt ttatccccgg gttctgtgga taacagggtt
     1921 agcgtttttg agaaaggaga gtccgctcgc cgcagccgaa cgaccgagcg tagcgagtca
     1981 gtgagcgagg aagcggaaga gcgcctgaac tggcattttc tccttacgca tctgtgcggc
     2041 atttcacacc ggacatggcg cacttttcat acaatccgca ctgatgccga atggttaagc
     2101 cagtatacac tccgctatcg ctacgtgact gggtcagggc ttcgccccga caccccaaag
     2161 gtcaacggcc tgacgcgccc tggcgggctt gtctgctccc ggcatccgct cacagacaag
     2221 ctgtgaccgt ctccgggagc tgcatgtgtc agcggttttc accgtcatcc ccgaagcgtg
     2281 cgaggcaact gcggtaaagc tcatcggcgt ggtcgtgaag cgattcacag atgtctgcct
     2341 gttcatccgc gtccagttcg ttgagcttct ccagcagcgt taatgtctgg cctctgatga
     2401 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
     2461 gggttctgtt catgggggta atgataccga tgaaacgcga gaggatgctc acgatacggg
     2521 ttactgatga tgaacatgcc cggttactgg aacgctgtga gggtaaacaa ctggcggtat
     2581 ggatgcggcg ggtctgcctg ggtgagccgg ttgcccgttc cgggaagctg ccgacactgg
     2641 caccgccgtt actgcgtcag ctggccgcca tcgggaataa ccttaaccag acagcgagga
     2701 aggtgaacag cgggcagtgg tcttccggtg accgggttca ggtggtggcc gcactgatgg
     2761 ccatcgggga tgagctgcgc cggctgcgtc tggcggtcag ggaacagggg gcgcgggatg
     2821 atagttaagt ttcatgccag gggaaaaggt ggtggcagtg gtccggttga ttacctgctg
     2881 ggaagggagc gtaaccggga aggggcaacg gtgcttcagg ggaatccgga agaggtccgg
     2941 gaactcatcg atgccacgcc atttgcgaag aaatacacgt caggtgttct gtcgttcgcg
     3001 gagaaggagc tgccgcctgg agggcgtgaa aaagtgatgg cgagctttga gcgtgtactg
     3061 atgcccggtc tcgagaaaaa tcagtacagc atcctgtggg tggagcacca ggacaaggga
     3121 cggcttgagc tgaattttgt cattccgaac atggagctac agaccggaaa acgcctccag
     3181 ccgtactacg accgggcaga caggccgaga attgatgcct ggcagacgct ggtgaatcac
     3241 cattacggtc tgcatgaccc caacgccccg gagaaccgcc ggacgctgac ccttcctgat
     3301 aacctgccgg aaacgaaaca ggcgcttgct gagggcgtca cacgcgggat agacgcgctt
     3361 taccatgccg gagagataaa aggccgtcag gatgtgattc aggcgctcac agaggcgggg
     3421 cttgaagtgg tcagggtgac gcgaagcagt atcagcattg cagacccgaa cggcgggagg
     3481 aatatcagac tgaaaggagc gttttatgag caatctttta cagacgggcg cggagttcga
     3541 gaaaaagctg aaagagagag ccgaatctac agagaaaatg ctgaacgacg agttcaggaa
     3601 gctcgaagaa tctgtaaaca gggaactgac ctcaaacgaa tctttaatca ggaacgctat
     3661 caacgaccat acaacagccc tgaaagaact gctggagagg tatcagaaaa cgaccgtcga
     3721 tacgatggac gcgcactgga aaaccgtact gaaaatgagc gtgaaacgct ggctgtggct
     3781 gattatcgtc tcagtactga tgtttgccac gaccgggagt ctgctgtggt accagggcat
     3841 gaagataaac gcgaatatga atattctccg cgagcagaaa gagagtctgg agaagctgaa
     3901 cgcgaagaca tggggcgtgc gttatcacga ggacagcaac ggacgttttc tggtgttgcc
     3961 gaaggggatg aaagccgaaa cgaactggac gaaggacaac ggcaaactga acgcagtgag
     4021 actggtgcag gagtgacaga gcatgacgga gctggaaaga catttgctga acgcattaga
     4081 gcaactgcaa caggactata tgcagcggct gaacgaatgg gagaacgcct tcgtggaatt
     4141 gcagaaaatg ttttcgctta cgcaacggga caacgcgatg ctgaacgggc gagtcatgca
     4201 gttgagtcag caggtgcagc acttgagcga gcggacagaa cgcttgagcc agttatacag
     4261 cgagaacagg agataaggga tgaacggctg atgcaggaac gcgaacatga gttatctctg
     4321 gagcgggagc gtcagccgga aatcaggagc ggacactgga tggcccttcg ctgggatggt
     4381 gaaaccatga aaaatgacgt gttcagtgga ttaaggcggg ctaatgtggc ctgtagcatc
     4441 tggttgcata ggtattcatg cggttaaaat ttatcaggtg cgatcgcggc agtttttcgg
     4501 gtggtttgtt gccattttta cctgtctgct gccgtgatcg caccgaacgc gttttggcgg
     4561 tgcgtacaat gcgacgttat ggtaaatgga gcggattttc aggaaggagt gatactaaca
     4621 actcgaacac tgtgcaaata atgatttttt taaaatatcg gtaacgataa taaattattt
     4681 cgttttacgc cggtaataaa tataatgtag tttcagtata taaaaaaata tatgaggtgt
     4741 cttgtgggag ctttcatgaa tcttcaacaa cgtataaata aactaccaca attatcatcc
     4801 agtttttcat ttggaaaaga tattgataat attcattcat ttatctttaa cgaaacttct
     4861 aaagataaaa ttgaagattt actaagaaaa tgggtgtcag gcaatcagcc ctgtgtattt
     4921 ggtaaattgg caagcaagaa aattaaaggg cttgatttcc atttatctat tgtaaatagc
     4981 ccccaactat ataatgatga tggtcatttg tttgattttt tgcgtaatga aagagttcgt
     5041 tttaaagaac gcgcaagaag aggagaggtc agtgcgcatc tcatttattt tattcatcca
     5101 caacttgcct ttgctcgtcc atcagaagag ttagtggaca tacaaaaata tatttgttcg
     5161 ctgcatatgc cagagtgcta tccaataaaa gaggatgtta tttatactga aagcgtacca
     5221 tttcaggata aagatgggct aaaaatatat aaggctggtg ttaatgtttt ttactcttct
     5281 gctcatagaa caagaaatca tgatagaaga ataccagggg ggaatactta tttctgtaaa
     5341 tgccccgggg cattttatga ggctggcgat agaaaaaggt ttctataaag atcaagaaca
     5401 agcgttagcg gatattcgta acatgactat tcagtctgtt ggtaagggtg ggtattcaca
     5461 tccggaaggg atttctacaa catggcattc agaatctaat ttggatcgtc gttttggatg
     5521 cccagtgcac accggtaata gttcttatta cagcggtttt tatcatactg atgtattaat
     5581 tccaggagaa ttaactaagg atgaaagatt gctccatgaa atagaaaata gtgatccaat
     5641 gatcttcaac tggaatgtat tgttttatgt ttcattagaa gagtttccta tagatgatcc
     5701 ttattatgga gagtttattg gtattcctgt agatgatgca tctatgtttt ttaactcctt
     5761 tcaaccaagg aaatttgaaa acaaacccct ttatgagaaa gaggatgatt aatatgatta
     5821 aaggaatgct ttttgctttt ttagcagcgt tctcatgggg ggcagctatc gtaatgagca
     5881 aaaaaggagt tgaagaacta gatgccggag gggtgttttt ctggcagatt tgtagtgcag
     5941 taatactatc ttggatagtt ctttttataa aaagaaaaaa actcccatta aactcaaaag
     6001 cgatacttgc ttatgcgaca ggcgtttttg aaccattctt agcgtatact tttacactct
     6061 acggacttta ttttatttca gcaggaagcg catcaattat tttttcactt gaatcagtgt
     6121 ttatattggt gttgtctgtt cttatttgct cagttaaaat taattccccg caatatttta
     6181 tattgttcct tataggtgca attgttggca gtattttggt agccttgcca gacatcagta
     6241 atggatcgaa taatattgtt ggatattgtc ttgttgttgc cggtgtgtta tcggcagcat
     6301 tttatgttgt tatatcgtct cgccttatta aatcgtttga gcctatcaca ttattgagcg
     6361 gacagttgac attctccctt atactgtcat cgctctttat ttttatatct aaatcatcaa
     6421 tttatcttcc gttggaatcc gtgattattg ttttttcatc tggaatatta caatattttt
     6481 tagcttttgt ttttacttga actccttgcg ttggctccca gttcatttgg cagggataat
     6541 gttgtacttt attccgctag ttgcattatt tttgtcatgg ttgtttttgg atgaacaatt
     6601 cacattctta cagggggtag gatttattgt aactgtgcta agtgtatatt ttcttaacag
     6661 aaaat
//
