LOCUS       NZ_CP083894             9459 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Escherichia coli strain NDM1 plasmid unnamed6, complete sequence.
ACCESSION   NZ_CP083894
VERSION     NZ_CP083894.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21398202
            Assembly: GCF_020149645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Simner,P., Mostafa,H., Bergman,Y., Ante,M., Tekle,T., Adebayo,A.,
            Beisken,S., Dzintars,K. and Tamma,P.D.
  TITLE     Progressive Development of Cefiderocol Resistance in Escherichia
            coli During Therapy Is Associated with Increased blaNDM-5 Copy
            Number and Gene Expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   Simner,P., Mostafa,H., Bergman,Y., Ante,M., Tekle,T., Adebayo,A.,
            Beisken,S., Dzintars,K. and Tamma,P.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) BioIT, Ares-Genetics, Karl-Farkas-Gasse 18,
            Vienna 1180, Austria
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Canu v. JAN-2021
            Assembly Name                     :: Isolate1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 67x
            Sequencing Technology :: Oxford Nanopore MiniION + Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 01:54:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,150
            CDSs (total)                      :: 5,024
            Genes (coding)                    :: 4,771
            CDSs (with protein)               :: 4,771
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 1 of 253
            Pseudo Genes (frameshifted)       :: 124 of 253
            Pseudo Genes (incomplete)         :: 145 of 253
            Pseudo Genes (internal stop)      :: 42 of 253
            Pseudo Genes (multiple problems)  :: 55 of 253
            Pseudo Genes (short protein)      :: 4 of 253
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083894.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NDM1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: Baltimore"
                     /lat_lon="39.296 N 76.591 W"
                     /collection_date="2020"
                     /collected_by="Johns Hopkins University"
                     /genotype="ST90"
     gene            complement(<778..918)
                     /locus_tag="LCE25_RS25430"
                     /old_locus_tag="LCE25_25430"
                     /pseudo=""
     CDS             complement(<778..918)
                     /locus_tag="LCE25_RS25430"
                     /old_locus_tag="LCE25_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001482311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1091..>1975
                     /locus_tag="LCE25_RS25435"
                     /old_locus_tag="LCE25_25435"
                     /pseudo=""
     CDS             1091..>1975
                     /locus_tag="LCE25_RS25435"
                     /old_locus_tag="LCE25_25435"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2013..2531
                     /locus_tag="LCE25_RS25440"
                     /old_locus_tag="LCE25_25440"
     CDS             2013..2531
                     /locus_tag="LCE25_RS25440"
                     /old_locus_tag="LCE25_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001749510.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            2538..2771
                     /locus_tag="LCE25_RS25445"
                     /old_locus_tag="LCE25_25445"
     CDS             2538..2771
                     /locus_tag="LCE25_RS25445"
                     /old_locus_tag="LCE25_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3171..3815
                     /locus_tag="LCE25_RS25450"
                     /old_locus_tag="LCE25_25450"
     CDS             3171..3815
                     /locus_tag="LCE25_RS25450"
                     /old_locus_tag="LCE25_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529705.1"
                     /GO_function="GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="WP_011017202.1"
                     /translation="MHNKKFDRSEHVYRNDSFLELIKDAVRFFSGTPVHSLPPPERFQG
                     AGVYALYYTGHYSLYDEYSRINRLAYNLPIYVGKAVPAGWRQSRISDHETRAGSELSNR
                     IREHSRNIAKTSNLNLCDFSCRFVIFEATGSDMISTVEAALIKIYKPLWNTVVDGFGNH
                     TPGAGRFAQAKSDWDVIHPGREWAEKCTGVHSEPYFIEERIKQYFSKSNFT"
                     /name="Eco29kI family restriction endonuclease"
     gene            3851..4963
                     /locus_tag="LCE25_RS25455"
                     /old_locus_tag="LCE25_25455"
     CDS             3851..4963
                     /locus_tag="LCE25_RS25455"
                     /old_locus_tag="LCE25_25455"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_224446639.1"
                     /translation="MAKGLELAGFQHVGFVELNKHACDSLRLNFDEEKVFQGDIKNYDL
                     SSIDKIDIVAGGPPCQPFSLGGKHKAHDDSRDMFPFAIKAIEVLQPKAFIFENVKGLLR
                     KSFADYFEYIILRLTYPCLSLSNVSDWRIHLDALRKARKDKHCGCTYDVSFKLINAADY
                     GVPQIRERVIIVGVRSDLNKKWSFPSVTHTQEKLLWEQHVTGDYWRKHNIDAVIDKGIS
                     ERLMKRFGIFPPSGKSWVTVRDALKNVPQPYESHGIGDHIFKDGARVYPGHTGSYIDLP
                     SKTLKAGAHGVPGGENMIRYEDGTVRYFTVYEGKLLQTFPREFLIAGAWGEAMRQIGNA
                     VPVLLAQKIGQQLYNLLNEEVEPLLCSNFN"
                     /name="DNA cytosine methyltransferase"
     gene            complement(5199..5357)
                     /locus_tag="LCE25_RS25890"
     CDS             complement(5199..5357)
                     /locus_tag="LCE25_RS25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071791734.1"
                     /translation="MHEPPVQPDCCALPGNYRLESNPVRHALTPLAAAIGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(6026..6217)
                     /locus_tag="LCE25_RS25460"
                     /old_locus_tag="LCE25_25460"
     CDS             complement(6026..6217)
                     /locus_tag="LCE25_RS25460"
                     /old_locus_tag="LCE25_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6281..6604
                     /locus_tag="LCE25_RS25465"
                     /old_locus_tag="LCE25_25465"
     CDS             6281..6604
                     /locus_tag="LCE25_RS25465"
                     /old_locus_tag="LCE25_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956000.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6813..>7244
                     /locus_tag="LCE25_RS25895"
                     /pseudo=""
     CDS             6813..>7244
                     /locus_tag="LCE25_RS25895"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            7282..7801
                     /locus_tag="LCE25_RS25475"
                     /old_locus_tag="LCE25_25475"
                     /pseudo=""
     CDS             7282..7801
                     /locus_tag="LCE25_RS25475"
                     /old_locus_tag="LCE25_25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /name="MbeB family mobilization protein"
     gene            7808..8041
                     /locus_tag="LCE25_RS25480"
                     /old_locus_tag="LCE25_25480"
     CDS             7808..8041
                     /locus_tag="LCE25_RS25480"
                     /old_locus_tag="LCE25_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            8581..8802
                     /locus_tag="LCE25_RS25485"
                     /old_locus_tag="LCE25_25485"
     CDS             8581..8802
                     /locus_tag="LCE25_RS25485"
                     /old_locus_tag="LCE25_25485"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="WP_224446638.1"
                     /translation="MYALYYTGHYSLYDEYSRINRLAYNLPIYVGKAVPAGWRQSRISD
                     HETRAGSELSNRIREHSRNIAKTSNLNL"
                     /name="Eco29kI family restriction endonuclease"
     gene            <8971..9025
                     /locus_tag="LCE25_RS25490"
                     /old_locus_tag="LCE25_25490"
                     /pseudo=""
     CDS             <8971..9025
                     /locus_tag="LCE25_RS25490"
                     /old_locus_tag="LCE25_25490"
                     /inference="COORDINATES: protein motif:HMM:NF021064.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            9083..>9458
                     /gene="dcm"
                     /locus_tag="LCE25_RS25495"
                     /old_locus_tag="LCE25_25495"
                     /pseudo=""
     CDS             9083..>9458
                     /gene="dcm"
                     /locus_tag="LCE25_RS25495"
                     /old_locus_tag="LCE25_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712210.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /name="dcm"
     Typing          588..673
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          5856..5941
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          complement(73..481)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.786"
                     /coverage="95.0"
                     /evalue="3.01e-144"
     Typing          complement(5286..5831)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.192"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          984..1318
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.321"
                     /coverage="99.0"
                     /evalue="1.07e-65"
     Typing          1326..2875
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.565"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6595..7355
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.063"
                     /coverage="100.0"
                     /evalue="1.47e-157"
ORIGIN
        1 gtccttctag tgtagcctca gttagagcca ccgctttaaa actcataact gtctcaatcc
       61 accttgtcca gttactaatg gctgctgcac cagtggcgtt aaaatgtctt accgggttgg
      121 actcggacgt ttgtgatagt taccgggcaa ggcgcagcag tcgggctgaa caggggttcg
      181 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgaataccaa cagcgtgagc
      241 tatgagaaag cgcccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      301 agggtccgga acagggagag cgcacgaggg ggcttccagg aaacgcctgg tatctttata
      361 gtcctgtcgg gtttcgccac ctctgacttg agcgtctgtt tttgtgatgc tcgtcagggg
      421 ggcggagccc tatggaaaaa cgccagcggc gtggcctttt caggttcttg tttttgctca
      481 catgtttctt ttggcgttga agcggaagag cgcctgatgc gtgcattttt tctccttacg
      541 catctgtgcg gtatttcaca ccgtatatgg tgctcttttc ttattatctg cactgatgcc
      601 gcatggttaa gccagtatac actccgctat cgctacgtga ctgggtcagg gctgcgcccc
      661 gacacccgct aaaacctgct gacgcgccct gacgggcttg tctgctcccg gcattcgctc
      721 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatccc
      781 gaaacgtgcg aggcagctgc ggtaaagctc atcggcgtgg tcgtgaagcg attcacaaat
      841 atcggcctgt tcatctgcgt ccagttcgtt gagcttctcc agcagcgtta atgtctggct
      901 tctgataaag cgggccatgt taagggcggt ttttttcctg tttagtcact gatgcctccg
      961 tgtaaggggg atttctgttc atggggtaat gataccgatg aaacgcgaga ggatgctcac
     1021 aatacgggtt actgatgatg aacatgcccg gttacttgaa cgctgtgagg gtaaacaact
     1081 ggcggtatgg atgcggcggg tctgcctggg tgagccggtt gcccgttccg gaaaactgcc
     1141 gacactggca ccgccgttac tgcgtcagct ggccgccatc ggaaataacc tgaatcagac
     1201 agcccgtaag gtgaacagcg ggcagtggtc ttccggtgac cgggttcagg tggtggccgc
     1261 actgatggcc atcggggatg agctgcgccg gctgcgtctg gccgtcaggg aacggggacg
     1321 cgggatgata gttaagtttc atgccagggg aaaaggtggt ggcagtggtc cggttgatta
     1381 cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta atccggaaga
     1441 agtacgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg gtgtgctgtc
     1501 gttcgcggag aaggagctgc caccgggagg acgtgaaaaa gtgatggcga gctttgagcg
     1561 tgtactgatg cccggtctcg aaaaaaatca gtacagcatc ctgtgggtgg agcaccagga
     1621 caagggacgg cttgagctga attttgtcat tccgaacatg gagctacaga ccggaaaacg
     1681 tctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc agacgctggt
     1741 gaatcaccat tacggactgc atgaccccaa cgccccggag aaccgccgga cgctgaccct
     1801 tcctgataac ctgccggaaa cgaaacaggc acttgctgag agcgttacac gcggtataga
     1861 cgcactttac catgccggag agataaaagg ccgtcaggat gtgattcagg cgctcacaga
     1921 ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag acccgaacgg
     1981 cgggaagaat atcaggctga aaggagcatt ttatgagcaa tcttttacag acgggcgcgg
     2041 agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg aacgacgagt
     2101 tcaggaagct cggcgagtct gtcagcgagg ctgtgacctc aaacgagacg aaaatcagag
     2161 acgctatagc cctgttcaca gcctcgacag agaaatcgct ggaaaaacac cgggaagggg
     2221 tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg aatacgggca
     2281 tgatgttact ggggatagtc tttctcctgt ttaccgcgag tggcgggacg ctctggtatc
     2341 ttggcggacg gatacagacg aacctggaag aaatcaggaa gcaggaagag acattgcaga
     2401 aactgaacgc gaagacatgg ggcgtggagt ttgtgcagga cgggaacagg aaattccttg
     2461 tccttccgta cgggaaatca gcggaggtga ttccctttca ggggaaagag tgggtacatc
     2521 tgaaggagtg acacagagtg acagagctgg aaatacattt gctgaacgcc ttagagcagc
     2581 tgcaacagga ctatatgcag cggctgagcg aatgggagag cgccttcgtg gaattgcaga
     2641 agatgttttc gcttacgcaa cgggacaacg cgatgctgaa cgagcgggtc atgcagttga
     2701 gtcagcaggt gcagcacttg agcgagcaga cagaacgctt gagccagtta tacagcgaga
     2761 actggagata agagaggaac ggctgataca ggagcgcgaa catgcgttat ccctggaacg
     2821 ggagcgtcag ccggaaatac aggaacgcac gctggatggt ccttcgctgg gatggtgaaa
     2881 ccatgaaaaa tggcagcttc agtggattaa gaggggttaa tgtggcctgt accatctggt
     2941 tgcataggta ttcatgcggt taaaatttat caggcgcgat cgcggcagtt tttcgggtgg
     3001 tttgttgcca tttttacctg tctgctgccg tgatcgcgct gaacgcgttt tagcggtgcg
     3061 cgtaatgcga cgttatggta aatgtgtatg gttttgggta tcagctttat gatcgtatca
     3121 taggtgatgt tgagtaggag tgttaatata aaaacctttc ggaaaacata atgcacaata
     3181 agaaatttga tagatctgag catgtttata gaaatgactc tttcctagag ttaataaaag
     3241 atgccgttcg tttttttagt ggtacgcctg ttcattcatt accaccaccg gaacggtttc
     3301 aaggtgctgg ggtgtatgct ctttactata cagggcatta ttcattatat gatgaatact
     3361 ctcgtataaa taggttggca tataaccttc ctatttatgt tggcaaggct gttcctgctg
     3421 gttggcgtca gtctagaatt agtgatcatg aaactagggc tggttctgaa ctatctaata
     3481 gaattagaga acatagccga aatatagcaa aaacatcgaa tctaaatttg tgtgattttt
     3541 catgtcgctt tgttatattt gaggctacgg ggtctgatat gattagtaca gttgaggccg
     3601 ctcttattaa aatttataag cctttgtgga atacagttgt tgatggcttt ggaaatcata
     3661 cccctggtgc cgggcgattt gcgcaagcta aatctgattg ggatgtaata catcctggca
     3721 gggagtgggc tgagaaatgt acaggtgttc attctgagcc atactttatt gaggagagaa
     3781 ttaaacagta tttttctaag agtaatttta cataatgaga tcattagaga ttttttcagg
     3841 agccggcggt ttggctaaag gtctagagtt ggcaggattt cagcatgtag gatttgttga
     3901 gttaaacaag catgcatgtg attctttgcg acttaatttt gatgaggaga aggtatttca
     3961 gggagatatt aaaaattatg acctttcatc tatagataaa atagatattg ttgctggcgg
     4021 gcctccctgc caaccatttt cattgggcgg aaaacataaa gctcatgatg actctaggga
     4081 tatgttccca tttgcaataa aagcaattga agtattgcaa ccaaaagctt ttatttttga
     4141 aaatgttaaa gggttgctta gaaaatcatt tgctgattat tttgaataca taattcttcg
     4201 tttgacttat ccttgtttat cgttaagtaa tgtaagtgat tggagaatac atctagatgc
     4261 attaagaaaa gctcgcaagg ataaacactg tgggtgtaca tatgatgtca gttttaaatt
     4321 gatcaatgca gctgattatg gagttccaca aattagggaa cgggtcataa ttgttggtgt
     4381 tcgaagtgat ttgaataaaa aatggtcttt tccaagcgta acgcatactc aagaaaaatt
     4441 actgtgggaa cagcatgtta ctggtgacta ttggcgaaag cataatattg atgcggttat
     4501 agataaaggt atttctgagc gtttaatgaa acgttttggt attttccccc catcggggaa
     4561 aagctgggtt actgttaggg acgcattaaa gaatgttcct caaccatatg agtcacatgg
     4621 tattggagat catatcttta aagatggcgc tcgagtttat cctggtcata ctggaagtta
     4681 tatcgatttg ccttcaaaaa cacttaaggc tggcgctcat ggcgtacctg gaggtgaaaa
     4741 tatgattagg tatgaggatg ggactgttcg ttattttacg gtttatgaag ggaagcttct
     4801 tcagactttt cctcgagagt ttctgattgc aggagcttgg ggggaggcta tgaggcagat
     4861 tggtaatgca gttcctgtgt tgctagctca gaagatagga cagcaattgt ataacttatt
     4921 gaatgaagag gttgagcctt tattgtgctc taacttcaat taaaagtttg tgtctctatg
     4981 gtggtgatac agccatagag gcaatttgca aaaaaagatc aaaggatctt cttgagatcc
     5041 tttttttctg cgcgtaatct gctgcctgta aacaaaaaaa ccaccgctac caacggtggt
     5101 tcgtttgccg gaatcaagag ctaccaactc tttttcccga aggtaactgg cttggtggag
     5161 cgcagatacc aaatactgtc cttctagtgt agcctcagtt aggccaccac ttcaagaact
     5221 cttaatatct caatccacct tgtccagtta ccaatggctg ctgccagtgg cgttaaggcg
     5281 tgtcttaccg ggttggactc aagacgatag ttaccgggca aggcgcagca gtcgggctga
     5341 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5401 caacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5461 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5521 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtct gtttttgtga
     5581 tgctcgtcag gggggcggag cctatggaaa aacgccagcg gcgtggcctt ttccgggttc
     5641 ttgttttttg ctcacatgtt tcttttggcg ttatcccctg attctgtgga taaccgcatt
     5701 accgtctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca
     5761 gtgagcgagg aagcggaaga gcgcctgatg cggcattttc tccttacgca tctgtgcggt
     5821 atttcacacc gtatatggtg ctcttttctt attatctgca ctgatgccgc atggttaagc
     5881 cagtatacac tccgctatcg ctacgtgact gggtcagggc tgcgccccga cacccgctaa
     5941 aacctgctga cgcgccctga cgggcttgtc tgctcccggc attcgctcac agacaagctg
     6001 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatccccg aaacgtgcga
     6061 ggcagctgcg gtaaagctca tcggcgtggt cgtgaagcga ttcacaaata tcggcctgtt
     6121 catctgcgtc cagttcgttg agcttctcca gcagcgttaa tgtctggctt ctgataaagc
     6181 gggccatgtt aagggcggtt ttttcctgtt tagtcactga tgcctccgtg taagggggat
     6241 ttctgttcat ggggtaatga taccgatgaa acgcgagagg atgctcacaa tacgggttac
     6301 tgatgatgaa catgcccggt tacttgaacg ctgtgagggt aaacaactgg cggtatggat
     6361 gcggcgggtc tgcctgggtg agccggttgc ccgttccgga aaactgccga cactggcacc
     6421 gccgttactg cgtcagctgg ccgccatcgg aaataacctg aatcagacag cccgtaaggt
     6481 gaacagcggg cagtggtctt ccggtgaccg ggttcaggtg gtggccgcac tgatggccat
     6541 cggggatgag ctgcgccggc tgcgtctggc cgtcagggaa caggggacgc gggatgatag
     6601 ttaagtttca tgccagggga aaaggtggtg gcagtggtcc ggttgattac ctgctgggaa
     6661 gggagcgtaa ccgggaaggg gcaacggtgc ttcagggtaa tccggaagaa gtacgggaac
     6721 tcatcgatgc cacgccattt gcgaagaaat acacgtccgg tgtgctgtcg ttcgcggaga
     6781 aggagctgcc accgggagga cgtgaaaaag tgatggcgag ctttgagcgt gtactgatgc
     6841 ccggtctcga aaaaaatcag tacagcatcc tgtgggtgga gcaccaggac aagggacggc
     6901 ttgagctgaa ttttgtcatt ccgaacatgg agctacagac cggaaaacgt ctccagccgt
     6961 actacgaccg cgcagacagg ccgagaattg atgcctggca gacgctggtg aatcaccatt
     7021 acggactgca tgaccccaac gccccggaga accgccggac gctgaccctt cctgataacc
     7081 tgccggaaac gaaacaggca cttgctgaga gcgttacacg cggtatagac gcactttacc
     7141 atgccggaga gataaaaggc cgtcaggatg tgattcaggc gctcacagag gcggggcttg
     7201 aagtggtcag ggtgacgcga agcagtatca gcattgcaga cccgaacggc gggaagaata
     7261 tcaggctgaa aggagcattt tatgagcaat cttttacaga cgggcgcgga gttcgagaaa
     7321 aaagctgaaa gagagagccg aatctacaga gaaaatgctg aacgacgagt tcaggaagct
     7381 cggcgagtct gtcagcgagg ctgtgacctc aaacgagacg aaaatcagag acgctatagc
     7441 cctgttcaca gcctcgacag agaaatcgct ggaaaaacac cgggaagggg tgaaagaggc
     7501 gatgatgcag cacaggaagg acgtgttaaa gctggcaggg aatacgggca tgatgttact
     7561 ggggatagtc tttctcctgt ttaccgcgag tggcgggacg ctctggtatc ttggcggacg
     7621 gatacagacg aacctggaag aaatcaggaa gcaggaagag acattgcaga aactgaacgc
     7681 gaagacatgg ggcgtggagt ttgtgcagga cgggaacagg aaattccttg tccttccgta
     7741 cgggaaatca gcggaggtga ttccctttca ggggaaagag tgggtacatc tgaaggagtg
     7801 acacagagtg acagagctgg aaatacattt gctgaacgcc ttagagcagc tgcaacagga
     7861 ctatatgcag cggctgagcg aatgggagag cgccttcgtg gaattgcaga agatgttttc
     7921 gcttacgcaa cgggacaacg cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt
     7981 gcagcacttg agcgagcaga cagaacgctt gagccagtta tacagcgaga actggagata
     8041 agagaggaac ggctgataca ggagcgcgaa catgcgttat ccctggaacg ggagcgtcag
     8101 ccggaaatac aggaacgcac gctggatggt ccttcgctgg gatggtgaaa ccatgaaaaa
     8161 tggcagcttc agtggattaa gaggggttaa tgtggcctgt accatctggt tgcataggta
     8221 ttcatgcggt taaaatttat caggcgcgat cgcggcagtt tttcgggtgg tttgttgcca
     8281 tttttacctg tctgctgccg tgatcgcgct gaacgcgttt tagcggtgcg cgtaatgcga
     8341 cgttatggta aatgtgtatg gttttgggta tcagctttat gatcgtatca taggtgatgt
     8401 tgagtaggag tgttaatata aaaacctttc ggaaaacata atgcacaata agaaatttga
     8461 tagatctgag catgtttata gaaatgactc tttcctagag ttaataaaag atgccgttcg
     8521 ttttttagtg gtacgcctgt tcattcatta ccaccaccgg aacggtttca aggtgctggg
     8581 gtgtatgctc tttactatac agggcattat tcattatatg atgaatactc tcgtataaat
     8641 aggttggcat ataaccttcc tatttatgtt ggcaaggctg ttcctgctgg ttggcgtcag
     8701 tctagaatta gtgatcatga aactagggct ggttctgaac tatctaatag aattagagaa
     8761 catagccgaa atatagcaaa aacatcgaat ctaaatttgt gatttttcat gtcgctttgt
     8821 tatatttgag gctacggggt ctgatatgat tagtacagtt gaggccgctc ttattaaaat
     8881 ttataagcct ttcccggaat acagttgttg atggctttgg aaatcatacc ctggtgccgg
     8941 gcgatttgcg caagctaaat ctgattggga tgtaatacat cctggcaggg agtgggctga
     9001 gaaatgtaca ggtgttcatt cttgagccat actttattga ggagagaatt aaacagtatt
     9061 tttctaagag taattttaca taatgagatc attagagatt ttttcagagc cggcggtttg
     9121 gctaaaggtc tagagttggc aggatttcag catgtaggat ttgttgagtt aaacaagcat
     9181 gcatgtgatt ctttgcgact taatttttga tgaggagaag gtatttcagg gagatattaa
     9241 aaattatgac ctttcatcta tagataaaat agatattgtt gctggcgggc ctccctgcca
     9301 accattttca ttgggcggaa aacataaagc tcatgatgac tctagggata tgttcccatt
     9361 tgcaataaaa gcaattgaag tattgcaacc aaaagctttt attttttgaa aatgttaaag
     9421 agttgcttag aaaatcattt gctgattatt ttgaataca
//
