LOCUS       NZ_CP083893            11489 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Escherichia coli strain NDM1 plasmid unnamed5, complete sequence.
ACCESSION   NZ_CP083893
VERSION     NZ_CP083893.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21398202
            Assembly: GCF_020149645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Simner,P., Mostafa,H., Bergman,Y., Ante,M., Tekle,T., Adebayo,A.,
            Beisken,S., Dzintars,K. and Tamma,P.D.
  TITLE     Progressive Development of Cefiderocol Resistance in Escherichia
            coli During Therapy Is Associated with Increased blaNDM-5 Copy
            Number and Gene Expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11489)
  AUTHORS   Simner,P., Mostafa,H., Bergman,Y., Ante,M., Tekle,T., Adebayo,A.,
            Beisken,S., Dzintars,K. and Tamma,P.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) BioIT, Ares-Genetics, Karl-Farkas-Gasse 18,
            Vienna 1180, Austria
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Canu v. JAN-2021
            Assembly Name                     :: Isolate1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 67x
            Sequencing Technology :: Oxford Nanopore MiniION + Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 01:54:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,150
            CDSs (total)                      :: 5,024
            Genes (coding)                    :: 4,771
            CDSs (with protein)               :: 4,771
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 1 of 253
            Pseudo Genes (frameshifted)       :: 124 of 253
            Pseudo Genes (incomplete)         :: 145 of 253
            Pseudo Genes (internal stop)      :: 42 of 253
            Pseudo Genes (multiple problems)  :: 55 of 253
            Pseudo Genes (short protein)      :: 4 of 253
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083893.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NDM1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: Baltimore"
                     /lat_lon="39.296 N 76.591 W"
                     /collection_date="2020"
                     /collected_by="Johns Hopkins University"
                     /genotype="ST90"
     gene            complement(1472..1658)
                     /locus_tag="LCE25_RS25360"
                     /old_locus_tag="LCE25_25360"
                     /pseudo=""
     CDS             complement(1472..1658)
                     /locus_tag="LCE25_RS25360"
                     /old_locus_tag="LCE25_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1722..2044
                     /locus_tag="LCE25_RS25365"
                     /old_locus_tag="LCE25_25365"
                     /pseudo=""
     CDS             1722..2044
                     /locus_tag="LCE25_RS25365"
                     /old_locus_tag="LCE25_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2034..2348
                     /locus_tag="LCE25_RS25370"
                     /old_locus_tag="LCE25_25370"
     CDS             2034..2348
                     /locus_tag="LCE25_RS25370"
                     /old_locus_tag="LCE25_25370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224446634.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELID
                     ATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKISTASCGWSTRTRDGLS
                     "
                     /name="hypothetical protein"
     gene            2315..>2683
                     /locus_tag="LCE25_RS25375"
                     /old_locus_tag="LCE25_25375"
                     /pseudo=""
     CDS             2315..>2683
                     /locus_tag="LCE25_RS25375"
                     /old_locus_tag="LCE25_25375"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2721..3239
                     /locus_tag="LCE25_RS25380"
                     /old_locus_tag="LCE25_25380"
     CDS             2721..3239
                     /locus_tag="LCE25_RS25380"
                     /old_locus_tag="LCE25_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328227.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            3246..3479
                     /locus_tag="LCE25_RS25385"
                     /old_locus_tag="LCE25_25385"
     CDS             3246..3479
                     /locus_tag="LCE25_RS25385"
                     /old_locus_tag="LCE25_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_011117658.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSRTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3510..3671
                     /locus_tag="LCE25_RS25865"
     CDS             3510..3671
                     /locus_tag="LCE25_RS25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_073481910.1"
                     /translation="MRYPWSVSASWKYRNAHWMVLRWDGETMKNGRLSGLRQANVVCTI
                     WLHRYSCG"
                     /name="mobilization protein"
     gene            complement(4750..5487)
                     /locus_tag="LCE25_RS25390"
                     /old_locus_tag="LCE25_25390"
     CDS             complement(4750..5487)
                     /locus_tag="LCE25_RS25390"
                     /old_locus_tag="LCE25_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme EcoRV"
                     /protein_id="WP_011117659.1"
                     /translation="MSLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFE
                     LFSRPIINKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENEKIK
                     FTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVATRKSSLKTYNINELNEIPKPY
                     KGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDSEDEFLDYWRNYERT
                     SQLRNDKYNNISEYRNWIYRGRK"
                     /name="type-2 restriction enzyme EcoRV"
     gene            complement(5471..5752)
                     /locus_tag="LCE25_RS25395"
                     /old_locus_tag="LCE25_25395"
     CDS             complement(5471..5752)
                     /locus_tag="LCE25_RS25395"
                     /old_locus_tag="LCE25_25395"
                     /inference="COORDINATES: protein motif:HMM:NF024252.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_197673838.1"
                     /translation="MGYPILGCIIYKKNDGKSMSVREIIKKNRVICKLSQGEVAKALGK
                     PQSYISKIEQGERRVDVDEFIAICFAIGIDPINTLKEVIMEKKHESSF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            5794..6690
                     /locus_tag="LCE25_RS25400"
                     /old_locus_tag="LCE25_25400"
     CDS             5794..6690
                     /locus_tag="LCE25_RS25400"
                     /old_locus_tag="LCE25_25400"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="WP_001570854.1"
                     /translation="MKDKVFVPPIKSQGIKTKLVPWIKRIVPKNFNGVWVEPFMGTGVV
                     AFNVAPKDALLCDTNPHLISFYNALKNKDITGDLVKDFLYREGEKLLLSNGEYYYEVRE
                     RFNNYKEPLDFLFLNRSCFNGMIRFNSKGGFNVPFCKKPNRFAQAYITKISNQVDRISE
                     IISKGNYTFLCQSFEKTIGMVNRDDVVYCDPPYIGRHVDYFNSWGERDERLLFETLSSL
                     NATFITSTWHHNDYRENKYVRDLWSSFRILTKEHFYHVGASEKNRSPMVEALITNIAKD
                     IIDHIEKSSGDILVIEE"
                     /name="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
     gene            complement(6957..7088)
                     /locus_tag="LCE25_RS25870"
     CDS             complement(6957..7088)
                     /locus_tag="LCE25_RS25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071598030.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGRGCTRNC"
                     /name="replication initiation protein"
     gene            complement(7756..7947)
                     /locus_tag="LCE25_RS25405"
                     /old_locus_tag="LCE25_25405"
     CDS             complement(7756..7947)
                     /locus_tag="LCE25_RS25405"
                     /old_locus_tag="LCE25_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8012..8335
                     /locus_tag="LCE25_RS25410"
                     /old_locus_tag="LCE25_25410"
     CDS             8012..8335
                     /locus_tag="LCE25_RS25410"
                     /old_locus_tag="LCE25_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_069354221.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSCDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8544..>8939
                     /locus_tag="LCE25_RS25875"
                     /pseudo=""
     CDS             8544..>8939
                     /locus_tag="LCE25_RS25875"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            9018..9536
                     /locus_tag="LCE25_RS25880"
     CDS             9018..9536
                     /locus_tag="LCE25_RS25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328227.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            9543..9776
                     /locus_tag="LCE25_RS25420"
                     /old_locus_tag="LCE25_25420"
     CDS             9543..9776
                     /locus_tag="LCE25_RS25420"
                     /old_locus_tag="LCE25_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_011117658.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSRTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            9805..9966
                     /locus_tag="LCE25_RS25885"
     CDS             9805..9966
                     /locus_tag="LCE25_RS25885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_073481910.1"
                     /translation="MRYPWSVSASWKYRNAHWMVLRWDGETMKNGRLSGLRQANVVCTI
                     WLHRYSCG"
                     /name="mobilization protein"
     Typing          1299..1386
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          7585..7672
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(733..1275)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.818"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(7017..7561)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.446"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1693..1988
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.96"
                     /coverage="99.0"
                     /evalue="1.35e-56"
     Typing          2023..2294
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.516"
                     /coverage="97.0"
                     /evalue="4.62e-105"
     Typing          2292..3583
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.064"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8326..9101
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.189"
                     /coverage="100.0"
                     /evalue="1.68e-147"
ORIGIN
        1 aataatatca aaagggaact atacattctt gtgtcagtca tttgaaaaac tattggtggc
       61 gtggtaaaaa tagggatgat gttgtttatt gtgatcctcc atatattggt aggcatgttg
      121 attattttta attcatgggg gagtttatag agcgtttggt attatttgaa acgttatcat
      181 ctttgaatgc tacatttatg taacgttcac ggcatcataa tgattatagg aaggttacgt
      241 tcgtatttgc ggtcatcttt agaatattga taaaagaaac atttttatca tgttggagcg
      301 tccgaaaaaa acagatctga tggtagaaag gcgctaatta caaatatagc caaggatatt
      361 attgatcaca ttgaaaaaag tagtggggat attttggtaa ttgaagagtg atggagatga
      421 ggacataaag tattgaccac tcctgtacgt ttatcgtgag gggatatgta aaaagatctc
      481 aagatcctct tgagatcctt ttcgcgcgta atctgctgtc tgtagacgaa aaaaccaccc
      541 tggtggtttt tcgaagaagg ttaaataatc ctggcagatt atttaaccgt ggtaacaggg
      601 tgtacaagac cgctgccacc aaatctgtcc tttcagtgta gccgcagttg gtccttcact
      661 taagaaccta gtatcagcaa tttcttgtac atcctctacc agtggctgct gccagtggcg
      721 ttaggcgtgt cttacccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      781 cgggctgaac gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      841 gagatgcaaa cagcgagccg cgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      901 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      961 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     1021 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc ctcctgcgcg ccctttttct
     1081 gggtctttgt cttttgctca catgttcttt ccggcgttat cccctgattc tgtggataac
     1141 cgtttaccgc ctttgagtgc gctgacaccg ctcgccgcag tcgaacgacc gagcgtagcg
     1201 agtcagtgag cgaggaagcg gaagagcgcc tggacgtgca ttttctcctt acgcatctgt
     1261 gcggcatttc acacccggca tggcgtactt ttcatacaat ccgcactgat gccgcatggt
     1321 taagccagta tacactccgc tatcgctacg tgactgggtc agggctgcgc cccgacaccc
     1381 gctaaaacct gctgacgcgc cctgacgggc ttgtctgctc ccggagacgc tcacagacaa
     1441 gctgtgagcg tctccgggag ttgcatgtgt gtcagaggtt ttcaccgtca tccccgaaac
     1501 gtgcagctgc ggtaaagctc atcggcgtgg tcgtgaagcg attcacagat gtctgcctgt
     1561 tcatctgcgt ccagttcgtt gagcttctcc agcagcgtta atgtctggct tctgataaag
     1621 cgggccatgt taagggcggt tttttcctgt ttagtcactg atgcctccgt gtaaggggga
     1681 tttctgttca tggggtaatg ataccgatga aacgcgagag gatgctcacg atacgggtta
     1741 ctgatgatga acatgcacgg ttactggaac gctgtgaggg taaacagctg gcggtatgga
     1801 tgcggcgggt ctgcctgggt gagccggttg cccgttccgg gaagctgccg acactggcac
     1861 cgccgttact gcgccagctg gctgccatcg ggaataacct gaatcagacg gcgaggaagg
     1921 tgaacagcgg gcagtggtct tcctgtgacc gggttcaggt ggtggccgca ctgatggcca
     1981 tcggagagag ttgcgcagct tacggcaggt cgtcagggaa cagggggcgc gggatgatag
     2041 ttaaatttca tgccagggga aaaggtggtg gcagtggtcc ggttgattac ctgctgggga
     2101 gggagcgtaa ccgggaaggg gcaacggtgc ttcagggtaa tccggaagaa gtccgggaac
     2161 tcatcgatgc cacgccattt gcgaagaaat acacgtccgg tgttctgtcg ttcgcggaga
     2221 aggaactgcc gccgggagga cgtgaaaaag tgatggcgag ctttgagcgc gtactgatgc
     2281 ccggtctcga aaaaatcagt acagcatcct gtgggtggag caccaggaca agggacggct
     2341 tgagctgaat tttatcattc cgaacatgga gctacagacc ggaaaacgtc tccagccgta
     2401 ctacgaccgg gcagacaggc cgagaattga tgcctggcag acgctggtga atcaccatta
     2461 cgggctgcat gaccccaacg ccccggagaa ccgccggata ctgactctct ctgataacct
     2521 gccggaaacg aaacaggcgc ttgctgagag cgtcacacgc gggatagacg ctctttatca
     2581 tgtaggggag ataaaaggcc gtcaggatgt gattcaggcg ctcacagagg cggggcttga
     2641 agtggtcagg gtgacgcgaa gcagcatcag cattgcagac ccgaacggcg ggaagaatat
     2701 caggctgaaa ggagcgtttt atgagcaatc ttttacagac gggcgcggag ttcgagaaaa
     2761 agctgaaaga gagagccgaa tctacagaga aaatgctgaa cgacgagttc aggaagctcg
     2821 gcgagtctgt cagcgaggct gtgacctcaa acgagacgaa aatcagagac gctatagccc
     2881 tgttcacagc ctcgacagag aaatcgctgg aaaaacacag ggaaggggtg aaagaggcga
     2941 tgatgcagca caggaaggac gtgttaaagc tggcagggaa tacgggcatg atgttactgg
     3001 ggatagtctt tctcctgttt accgcgagtg gcgggacgct ctggtatctt ggcgggagga
     3061 tacaggcgaa cctggaagaa atcaggaagc aggaagagac attgcagaaa ctgaacgcga
     3121 agacatgggg cgtggagttt gtgcaggacg ggaacaggaa atttctggtc cttccgtacg
     3181 ggaaatcagc agaggtgatt ccctttcagg ggaaagagtg ggtacatctg aaggagtgac
     3241 acagaatgac agagctggaa acacatttgc tgaacgcctt agagcagctg caacaggact
     3301 atatgcagcg gctgagcgaa tgggagagcg ccttcgtgga attgcagaag atgttttcgc
     3361 gtacgcaacg ggacaacgcg atgctgaacg agcgggtcat gcagttgagt cagcaggtgc
     3421 agcacttgag cgagcagaca gaacgcttga gccagttata cagcgagaac tggagataag
     3481 agaggaacga ctgatacagg agcgcgaaca tgcgttatcc ctggagcgtg agcgccagct
     3541 ggaaatacag gaacgcacac tggatggtcc ttcgctggga tggtgaaact atgaaaaatg
     3601 gcaggttaag tggattaaga caggctaatg tggtctgtac catctggttg cataggtatt
     3661 catgcggtta aaatttatca ggcgcgattg cggcagtttt tcgggtggtt tgttgccatt
     3721 tttacctgtc tgctgctgtg atcgcgctga acgcgttttg gcggtgcgta caatttgtaa
     3781 ttatgttaaa tataatagat aagctcaggg tgggggccta tggcttatat ttgactataa
     3841 agctatttat catttatttt ccatctcgat gcattattaa taaatttctt ttattacaat
     3901 aaaagacgag gatatgattt acttttagag tatgtgattt gataggcaat gctgttgata
     3961 tatagcattt aatgcttatc ctgtattggg gttttaaatt atacattatt taaatactaa
     4021 actctctaag agtcggaggt ttttgatgtt gtaaataaga atacatgagg actgatgcca
     4081 gtgattctaa tattaaacta tatcaagaaa tttcctcaac tgatgtattg attttttgta
     4141 agggcgcgtt tatgtaaaga ggtgtatgaa taacaagaag taaataaacg tcattatatt
     4201 acatataatg gttttctatt ttattttatt attgtgttcg ttgcaataaa agaagctgtt
     4261 tatataaaaa tatgcgtgtg gtttatgata agctaaaaat actttttgta attaacaaaa
     4321 atgtgttgat ttttattaag aatcaagtta caacggacat gggtgcgttt ctagtcgttt
     4381 atcattgtaa ccggtctcaa tgtgagaagc gcacccaatt cttattttcc gcaatgtata
     4441 gacatacaga aaaaattctc tctcattggt gttgaattaa acatcattca agtgatatgt
     4501 atatttattg tgcgaaatgc agagggaaaa tatagagagg catatgtttt tatgtcttta
     4561 tgttgttgtg gtgagagtgt aagggtgcat catgtcatct ttgaatgtta agtgtgatgt
     4621 gccggaggag ggaatagtct gatgctaaat tttttctttt agtatccctc cctccccctc
     4681 tttctctcat gagtcagaga ggttttattc tctgttagag gggggggata taaacatata
     4741 agctattgct tattttcttc ctcggtatat ccagtttctg tattcgctta tattattata
     4801 cttgtcattt cttaattgac tggttctttc ataatttctc caatagtcta aaaactcatc
     4861 ctctgagtca aatattcctt ttccttctac aaagtcttta tagtgggcat gaatgctacc
     4921 tatatttgtt gtgtttccag atcctgccaa atctccagca ataacccatt tatcttgtaa
     4981 gaaaaccttt acgcctttgt atggtttagg gatttcattg agttcattta tattatatgt
     5041 ttttaaagat gattttcttg tagcaactct tgtatataca tatccgatta tccaatgggc
     5101 gatatattgg tcaaatggat aaacaatatt ttttgtgttg tttcgtataa agctggtata
     5161 cccaccaaga gtgaacttga ttttttcgtt ttctttgttt gtatatgttg tttttatatc
     5221 tattgcaatt tttttatttg gttcgcttgg tttgtaaaga gtaaagtcag gataatgatt
     5281 ttgttgttta ggttcttcta caatataccc atgtttttct gctattttat ttattattgg
     5341 tcttgagaat aactcaaata ttgtgcttag aacttttgtg tcacttccca atgggtatat
     5401 ttttccttct gcagatatta ttccgcatac atcatatttt tgattttcat catatagtgc
     5461 attaattaaa tcagaacgaa gactcatgtt ttttttccat tattacttct ttaagtgtat
     5521 ttattgggtc aattcctatt gcaaaacaga tggctataaa ttcgtcaacg tcaacgcgtc
     5581 tttctccttg ttctatttta gatatgtagg attgtggctt tccaagtgcc ttggcaacct
     5641 ctccttgaga gagtttacaa attactctat ttttttttat gatttctctt acagacatag
     5701 atttaccatc atttttttta tatattatgc accccaaaat gggataaccc aaaatgggtt
     5761 tctttggcat attttttaca aatggttctt attatgaaag ataaagtttt tgttccgcct
     5821 ataaaatcgc aagggattaa gactaaactg gttccgtgga taaagagaat tgttcctaaa
     5881 aactttaatg gggtatgggt cgagccgttt atggggactg gagttgttgc ctttaacgtt
     5941 gctcctaaag atgcattatt atgtgataca aaccctcatt tgatttcttt ttataatgca
     6001 ttgaaaaata aagatataac aggggatctt gttaaggatt ttttatatcg tgaaggtgaa
     6061 aaactcttat taagtaatgg tgagtattat tatgaagtca gggaaaggtt taataattac
     6121 aaggaaccgt tagatttttt attcttaaat aggtcttgtt ttaatggtat gattaggttt
     6181 aatagtaaag gagggtttaa tgttcctttt tgtaaaaaac caaacagatt tgcacaagca
     6241 tatattacca agatttcgaa tcaagttgat aggatttctg aaataatatc aaaagggaac
     6301 tatacattct tgtgtcagtc atttgaaaaa actattggca tggtaaatag ggatgatgtt
     6361 gtttattgtg atcctccata tattggtagg catgttgatt attttaattc atggggagag
     6421 cgtgacgagc gtttattatt tgaaacgtta tcatctttga atgctacatt tataacgtct
     6481 acgtggcatc ataatgatta tagggaaaat aagtacgttc gtgatttatg gtcatctttt
     6541 agaatattga caaaagaaca tttttatcat gttggagcgt ccgaaaaaaa cagatccccg
     6601 atggtagagg cgctaattac aaatatagcc aaggatatta ttgatcacat tgaaaaaagt
     6661 agtggggata ttttggtaat tgaagagtga tggagatgag gacataaagt gaccactctg
     6721 tacgtttatc gtgagagggg atatgtaaaa agatctcaag atcctcttga gatccttttt
     6781 tttcgcgcgt aatctgctgt ctgtagacga aaaaaccacc ctggcaggtg gtttttcgaa
     6841 ggttaaataa tcctggcaga ttatttaacc gtggtaacag ggtgtacaag accgctgcca
     6901 ccaaatctgt cctttcagtg tagccgcagt tggtccttca cttcaagaac tacgtatcag
     6961 caatttcttg tacatcctct accagtggct gctgccagtg gcgttaaggc gtgtcttacc
     7021 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7081 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata ccaacagcgt
     7141 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7201 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7261 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7321 ggggggcgga gcctatggaa aaacgcctcc tgcgcgccct ttttctgggt ctttgtcttt
     7381 tgctcacatg ttctttccgg cgttatcccc tgattctgtg gataaccgtt taccgccttt
     7441 gagtgcgctg acaccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag
     7501 gaagcggaag agcgcctgga cgtgcatttt ctccttacgc atctgtgcgg catttcacac
     7561 ccggcatggc gtacttttca tacaatccgc actgatgccg catggttaag ccagtataca
     7621 ctccgctatc gctacgtgac tgggtcaggg ctgcgccccg acacccgcta aaacctgctg
     7681 acgcgccctg acgggcttgt ctgctcccgg agacgctcac agacaagctg tgagcgtctc
     7741 cgggagttgc atgtgtcaga ggttttcacc gtcatccccg aaacgtgcga ggcagctgcg
     7801 gtaaagctca tcggcgtggt cgtgaagcga ttcacagatg tctgcctgtt catctgcgtc
     7861 cagttcgttg agcttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt
     7921 aagggcggtt ttttcctgtt tagtcactga tgcctccgtg taagggggga tttctgttca
     7981 tggggtaatg ataccgatga aacgcgagag gatgctcacg atacgggtta ctgatgatga
     8041 acatgcacgg ttactggaac gctgtgaggg taaacagctg gcggtatgga tgcggcgggt
     8101 ctgcctgggt gagccggttg cccgttccgg gaagctgccg acactggcac cgccgttact
     8161 gcgccagctg gctgccatcg ggaataacct gaatcagacg gcgaggaagg tgaacagcgg
     8221 gcagtggtct tcctgtgacc gggttcaggt ggtggccgca ctgatggcca tcgagagaga
     8281 gttgcgcagc ttacggcagg tcgtcaggga acagggggcg cgggatgata gttaaatttc
     8341 atgccagggg aaaaggtggt ggcagtggtc cggttgatta cctgctgggg agggagcgta
     8401 accgggaagg ggcaacggtg cttcagggta atccggaaga agtccgggaa ctcatcgatg
     8461 ccacgccatt tgcgaagaaa tacacgtccg gtgttctgtc gttcgcggag aaggaactgc
     8521 cgccgggagg acgtgaaaaa gtgatggcga gctttgagcg cgtactgatg ccaggtctcg
     8581 aaaagaatca gtacagcatc ctgtgggtgg agcaccagga caagggacgg cttgagctga
     8641 attttatcat tccgaacatg gagctacaga ccggaaaacg tctccagccg tactacgacc
     8701 gggcagacag gccgagaatt gatgcctggc agacgctggt gaatcaccat tacgggctgc
     8761 atgaccccaa cgccccggag aaccgccgga tactgactct ctctgataac ctgccggaaa
     8821 cgaaacaggc gcttgctgag agcgtcacac gcgggataga cgctctttat catgtagggg
     8881 agataaaagg ccgtcaggat gtgattcagg cgctcacaga ggcggggctt gaagtggtca
     8941 gggtgacgcg aagcagcatc agcattgcag acccgaacgg cgggaagaat atcaggctga
     9001 aaggagcgaa gcgttttatg agcaatcttt tacagacggg cgcggagttc gagaaaaagc
     9061 tgaaagagag agccgaatct acagagaaaa tgctgaacga cgagttcagg aagctcggcg
     9121 agtctgtcag cgaggctgtg acctcaaacg agacgaaaat cagagacgct atagccctgt
     9181 tcacagcctc gacagagaaa tcgctggaaa aacacaggga aggggtgaaa gaggcgatga
     9241 tgcagcacag gaaggacgtg ttaaagctgg cagggaatac gggcatgatg ttactgggga
     9301 tagtctttct cctgtttacc gcgagtggcg ggacgctctg gtatcttggc gggaggatac
     9361 aggcgaacct ggaagaaatc aggaagcagg aagagacatt gcagaaactg aacgcgaaga
     9421 catggggcgt ggagtttgtg caggacggga acaggaaatt tctggtcctt ccgtacggga
     9481 aatcagcaga ggtgattccc tttcagggga aagagtgggt acatctgaag gagtgacaca
     9541 gaatgacaga gctggaaaca catttgctga acgccttaga gcagctgcaa caggactata
     9601 tgcagcggct gagcgaatgg gagagcgcct tcgtggaatt gcagaagatg ttttcgcgta
     9661 cgcaacggga caacgcgatg ctgaacgagc gggtcatgca gttgagtcag caggtgcagc
     9721 acttgagcga gcagacagaa cgcttgagcc agttatacag cgagaactgg agataagagg
     9781 aacgactgat acaggagcgc gaacatgcgt tatccctgga gcgtgagcgc cagctggaaa
     9841 tacaggaacg cacactggat ggtccttcgc tgggatggtg aaactatgaa aaatggcagg
     9901 ttaagtggat taagacaggc taatgtggtc tgtaccatct ggttgcatag gtattcatgc
     9961 ggttaaaatt tatcaggcgc gattgcggca gtttttcggg tggtttgttg ccatttttac
    10021 ctgtctgctg ctgtgatcgc gctgaacgcg ttttggcggt gcgtacaatt tgtaattatg
    10081 ttaaatataa tagataagct cagggtgggg gcctatggct tatatttgac tataaagcta
    10141 tttatcattt attttccatc tcgatgcatt attaataaat ttcttttatt acaataaaag
    10201 acgaggatat gatttacttt tagagtatgt gatttgatag gcaatgctgt tgatatatag
    10261 catttaatgc ttatcctgta ttggggtttt aaattataca ttatttaaat actaaactct
    10321 ctaagagtcg gaggagaagt gatgttgtaa ataagaatac atgaggactg atgccagtga
    10381 ttctaatatt aaactatatc aagaaatttc ctcaactgat gtattgattt tttgtaaggg
    10441 cgcgtttatg taaagaggtg tatgaataac aagaagtaaa taaacgtcat tatattacat
    10501 ataatggttt tctattttat tttattattg tgttcgttgc aataaaagaa gctgtttata
    10561 taaaaatatg cgtgtggttt atgataagct aaaaattttt ttgtaattaa caaaaatgtg
    10621 ttgattttta ttaagaatca agttacaacg gacatgggtg cgtttctagt cgtttatcat
    10681 tgtaaccggt ctcaatgtga gaagcgcacc caattcttat tttccgcaat gtatagacat
    10741 acagaaaaaa ttctctctca ttggtgttga attaaacatc attcaagtga tatgtatatt
    10801 tattgtgcga aatgcagagg gaaaatatag agaggcatat gtttttatgt ctttatgttg
    10861 ttgtggtgag agtgtaaggg tgcatcatgt catctttgaa tgttaagtgt gatgtgccgg
    10921 aggagggaat agtctgatgc taaatttttt cttttagtat ccctccctcc ccctctttct
    10981 ctcatgagtc agagaggttt tattctctgt tagagggggg ggatataaac atataagcta
    11041 ttgcttattt tcttcctcgg tatatccagt ttctgtattc gcttatatta ttatacttgt
    11101 catttcttaa ttgactggtt ctttcataat ttctccaata gtctaaaaac tcatcctctg
    11161 agtcaaatat tccttttcct tctacaaagt ctttatagtg ggcatgaatg ctacctatat
    11221 ttgttgtgtt tccagatcct gccaaatctc cagcaataac ccatttatct tgtaagaaaa
    11281 cctttacgcc tttgtatggt ttagggattt cattgagttc atttatatta tatgtttttt
    11341 aaagatgatt ttcttgtagc aactcttgta tatacatatc cgattatcca atgggcgata
    11401 tattggtcaa atggataaac aatatttttt tgtgttgttt cgtataaagc tggtataccc
    11461 accaagagtg aacttgattt tttcgtttt
//
