LOCUS       NZ_CP083892            10928 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Escherichia coli strain NDM1 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP083892
VERSION     NZ_CP083892.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21398202
            Assembly: GCF_020149645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Simner,P., Mostafa,H., Bergman,Y., Ante,M., Tekle,T., Adebayo,A.,
            Beisken,S., Dzintars,K. and Tamma,P.D.
  TITLE     Progressive Development of Cefiderocol Resistance in Escherichia
            coli During Therapy Is Associated with Increased blaNDM-5 Copy
            Number and Gene Expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10928)
  AUTHORS   Simner,P., Mostafa,H., Bergman,Y., Ante,M., Tekle,T., Adebayo,A.,
            Beisken,S., Dzintars,K. and Tamma,P.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) BioIT, Ares-Genetics, Karl-Farkas-Gasse 18,
            Vienna 1180, Austria
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Canu v. JAN-2021
            Assembly Name                     :: Isolate1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 67x
            Sequencing Technology :: Oxford Nanopore MiniION + Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 01:54:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,150
            CDSs (total)                      :: 5,024
            Genes (coding)                    :: 4,771
            CDSs (with protein)               :: 4,771
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 1 of 253
            Pseudo Genes (frameshifted)       :: 124 of 253
            Pseudo Genes (incomplete)         :: 145 of 253
            Pseudo Genes (internal stop)      :: 42 of 253
            Pseudo Genes (multiple problems)  :: 55 of 253
            Pseudo Genes (short protein)      :: 4 of 253
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083892.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NDM1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: Baltimore"
                     /lat_lon="39.296 N 76.591 W"
                     /collection_date="2020"
                     /collected_by="Johns Hopkins University"
                     /genotype="ST90"
     gene            400..594
                     /locus_tag="LCE25_RS25315"
                     /old_locus_tag="LCE25_25315"
     CDS             400..594
                     /locus_tag="LCE25_RS25315"
                     /old_locus_tag="LCE25_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041636.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADVQAAMCERLHELA
                     EELQNSIQVHFEAENETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1057..1878)
                     /locus_tag="LCE25_RS25320"
                     /old_locus_tag="LCE25_25320"
     CDS             complement(1057..1878)
                     /locus_tag="LCE25_RS25320"
                     /old_locus_tag="LCE25_25320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224446630.1"
                     /translation="MHRSIVSPLLSSLMKYRRDCVFFVATHELSLPAFFDNVKVVNVKN
                     CYFSEQREPIYWDFNVVEDYDELFNGVDEQTKIDILGARDKVLFVEGVTSSLDRDLYSA
                     IFPMVSVVSKQKCDLVELAVKGLRLNDEIHRVSAFGIIDNDNKVQAKVDNLKKDFIFSL
                     DVHSIESIYYHPRMIKFVIDFVKEANGIDNVDALFMEIHDFAIDAINEKRDHLCCRAVE
                     KTIRADLMSAIPKQQDIKNRIKFNKEIDIECYVNKEIAAFDAMIAERNLMR"
                     /name="hypothetical protein"
     gene            complement(1947..2507)
                     /locus_tag="LCE25_RS25325"
                     /old_locus_tag="LCE25_25325"
     CDS             complement(1947..2507)
                     /locus_tag="LCE25_RS25325"
                     /old_locus_tag="LCE25_25325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.3"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_224446631.1"
                     /translation="MIKVQLPTREHRLHELNLNYGDVVFFIGQNGSGKSSLLNNLFQKI
                     GSQSSVYVLAHRKNTFSSDIIDFSNSEYKNAQGWIFNEIKGSQCRYRVNYDSQMVAIPI
                     VKLKNKVIARALSFVDSFKSNNNQPAELIKSEVDVLNMVFADAKLSIEFFLDDEANVMA
                     RNIKGENPDVYPISMLSDGEKVH"
                     /name="ATP-binding cassette domain-containing protein"
     gene            complement(2504..>3094)
                     /locus_tag="LCE25_RS25330"
                     /old_locus_tag="LCE25_25330"
                     /pseudo=""
     CDS             complement(2504..>3094)
                     /locus_tag="LCE25_RS25330"
                     /old_locus_tag="LCE25_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3658..3789
                     /locus_tag="LCE25_RS25860"
     CDS             3658..3789
                     /locus_tag="LCE25_RS25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071525238.1"
                     /translation="MHDPPVQSDRCALSGNYHLESNPERHDLSPLAAAIGKGCTRDY"
                     /name="replication initiation protein"
     gene            4426..4941
                     /locus_tag="LCE25_RS25335"
                     /old_locus_tag="LCE25_25335"
     CDS             4426..4941
                     /locus_tag="LCE25_RS25335"
                     /old_locus_tag="LCE25_25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="WP_000675351.1"
                     /translation="MKFYTISSRYIEYLKGFEDKVPNSEDPNYQNPKAFIGIVLEIQGH
                     KYLAPLTSPKPWHSTIKESSPSFFKLHENGVPENQLGLINLKFMIPIIEAEVSLLDLNG
                     MPNTAYKRMLYKQLQFIRVNSDKIAEKSELLRKLVLQGKMQGTCNFSLLEEKYRDFGKE
                     ANDTEEGE"
                     /name="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
     gene            5355..6065
                     /locus_tag="LCE25_RS25340"
                     /old_locus_tag="LCE25_25340"
     CDS             5355..6065
                     /locus_tag="LCE25_RS25340"
                     /old_locus_tag="LCE25_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000967645.1"
                     /translation="MLYWKKMPSLWIGNRIAEFSDLDTAKAIAALKIYLTFCLFCKESD
                     SGHRTVELTFSNLCEIASLSRSLVNEGLKILYAKELIKNISPTARKKIYTVDVLENHND
                     GWCKLPFKGVVDEDKKISAFQSMHNRYPFELLALQTYMYLLYARDNNSDYTLARKKTIC
                     KKLKCKLPELNKAITYLIHIGLLDRVVKKSIENIPLEMFHDSYYFYIKTGSKSALTYRR
                     KSEPPVIPEADLPF"
                     /name="hypothetical protein"
     gene            6859..7053
                     /locus_tag="LCE25_RS25345"
                     /old_locus_tag="LCE25_25345"
     CDS             6859..7053
                     /locus_tag="LCE25_RS25345"
                     /old_locus_tag="LCE25_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041636.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADVQAAMCERLHELA
                     EELQNSIQVHFEAENETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7290..8969)
                     /locus_tag="LCE25_RS25350"
                     /old_locus_tag="LCE25_25350"
     CDS             complement(7290..8969)
                     /locus_tag="LCE25_RS25350"
                     /old_locus_tag="LCE25_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598747.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_001774712.1"
                     /translation="MIKVQLPTREHRLHELNLNYGDVVFFIGQNGSGKSSLLNNLFQKI
                     GSQSSVYVLAHRKNTFSSDIIDFSNSEYKNAQGWIFNEIKGSQCRYRVNYDSQMVAIPI
                     VKLKNKVIARALSFVDSFKSNNNQPAELIKSEVDVLNMVFADAKLSIEFFLDDEANVMA
                     RNIKGENPDVYPISMLSDGEKSALIIASEIICADENTTIFLDEPERHMHRSIVSPLLSS
                     LMKYRRDCVFFVATHELSLPAFFDNVKVVNVKNCYFSEQREPIYWDFNVVEDYDELFNG
                     VDEQTKIDILGARDKVLFVEGVTSSLDRDLYSAIFPMVSVVSKQKCDLVELAVKGLRLN
                     DEIHRVSAFGIIDNDNKVQAKVDNLKKDFIFSLDVHSIESIYYHPRMIKFVIDFVKEAN
                     GIDNVDALFMEIHDFAIDAINEKRDHLCCRAVEKTIRADLMSAIPKQQDIKNRIKFNKE
                     IDIECYVNKEIAAFDAMIAERNLDALVSRYPIRETNLLAHVAKKCGFADRSRYELNVIR
                     TIKSNADARAFVLSLLGGLDEAILGCSDTENSPADTHTVFAM"
                     /name="AAA family ATPase"
     gene            complement(8966..>9556)
                     /locus_tag="LCE25_RS25355"
                     /old_locus_tag="LCE25_25355"
                     /pseudo=""
     CDS             complement(8966..>9556)
                     /locus_tag="LCE25_RS25355"
                     /old_locus_tag="LCE25_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(2..144)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.275"
                     /coverage="89.0"
                     /evalue="1.03e-47"
     Typing          complement(266..364)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.0"
                     /coverage="100.0"
                     /evalue="2.89e-43"
     Typing          complement(6344..6599)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.186"
                     /coverage="89.0"
                     /evalue="1.23e-106"
     Typing          complement(6724..6823)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.02"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          3254..3826
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.828"
                     /coverage="90.0"
                     /evalue="5.34e-173"
     Typing          9716..10285
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.308"
                     /coverage="90.0"
                     /evalue="1.93e-167"
ORIGIN
        1 aaagcggtgt cggcgcggtg ttaatagccg cgccgacacc gctttttaaa tatcacaaag
       61 agaaagaaaa ctaatttgtc ataactctct atttataaag aaaaatcagc aaaaaaattg
      121 tttttgcgtg gggtatgggc atttcaggca tgagaacaga ctcgcgtttt tccgagtatc
      181 agcggaatta ttgattaaaa cactcgacag agcgaaattt tatgcaacag cggctgcacc
      241 ttttcagggg attatttatc agttgatatg ctgtgccatg atacgtccta aaaaaacagg
      301 acaggatgtg caattgtaat accgtcgccc gcaacgtctt acaattacca cctagtcagg
      361 gtccacccga cacccggtac tgacggagaa caacggaagg tgaacaaaca gcaacaaact
      421 gcgctgaata tggcgcgatt tatcaaaagc cagagcctga cactgcttga aaaactggat
      481 gctctggatg ccgacgtaca ggcggccatg tgtgaacgac tgcacgaact cgcagaagaa
      541 ctccagaaca gcatccaggt tcactttgag gccgaaaatg aaacaggaac ataacgaagc
      601 tgacgaaggc ggtcacggct tgcccgtgag cattttcgtc agcagacaag cccgtcaggg
      661 cgcgtcagca ggttttaaca ggtgtcgggg cgcagctgac ttcagtcacg tagcgatagc
      721 ggagtgtata ctgacttaat acagcaacta tcgtacatcg cggtcaagtc acgcacaaac
      781 aaaaagcccc gcaaaagcag ggctgttatc tgatataggt tgtttagtct cacatagcaa
      841 aaactgtatg ggtatcagct gggctatttt ccgtatctga acatcccaaa atagcttcat
      901 ctaaccctcc gaggagggat aaaacaaatg ctctagcgtc agcattactc ttgatcgtac
      961 gaatcacgtt taactcatat ctagagcgat cggcaaaacc acatttttta gctacatgtg
     1021 ccaacaaatt tgtttctcgg atagggtagc gtgagactaa cgcatcaaat ttcgttccgc
     1081 gatcattgca tcaaatgcag caatttcttt attaacataa cattcaatat caatttcttt
     1141 attaaactta atgcggttct taatatcttg ctgttttggt atggccgaca ttaagtctgc
     1201 ccttattgtt ttttcaactg cacggcagca taagtgatcg cgcttttcat ttatggcatc
     1261 aatagcgaag tcatgaattt ccataaataa ggcatcaaca ttatctatcc cattagcctc
     1321 ttttacaaag tcaatgacaa acttaatcat tctaggatga taataaatcg actcaataga
     1381 atgaacgtcg agactaaaaa tgaagtcttt ctttaagtta tcgaccttcg cctgcacctt
     1441 gttatcatta tcaatgatac caaacgccga tactcgatga atttcgtcat ttagtctcaa
     1501 gccttttacc gccaattcaa ccaaatcaca tttttgttta gatacaactg aaaccatagg
     1561 aaatatggcc gaatataaat cacggtcaag gctagacgta acaccttcaa caaatagaac
     1621 cttatcccta gcgccaagta tatctatttt agtttgctca tcaacaccat tgaataattc
     1681 gtcataatcc tcaacaacat tgaaatccca gtatatcggt tctctttgtt cggaaaaata
     1741 gcagttttta acgttaacca ctttcacgtt atcaaaaaac gcaggcaagc ttaactcgtg
     1801 ggtggcaaca aaaaaaacac aatctctacg atatttcatt aaagatgaaa gcagcggaga
     1861 cacaatgctt ctgtgcatat gtcgctcagg ctcatctaag aaaatagttg tgttttcatc
     1921 agcgcaaata atctcactgg caataattag tgcacttttt caccgtcaga aagcattgat
     1981 ataggataaa catcaggatt ttcaccttta atattccttg ccataacgtt ggcttcatca
     2041 tctagaaaga actcaataga taatttggca tcagcaaaaa ccatgtttag cacatcaacc
     2101 tctgacttta ttaactctgc cggctgattg ttatttgatt taaatgaatc tacgaaactt
     2161 aaagccctgg ctatcacttt gttttttaat tttactatag gtatggcaac catctgtgag
     2221 tcataattaa ctctgtatct acattgactg cctttaattt cattaaaaat ccatccctgc
     2281 gcatttttat actcactatt actgaaatca ataatgtcgc tgctgaatgt attttttcta
     2341 tgcgctaaaa catatactga tgattgagat cctatttttt gaaaaagatt atttaatagg
     2401 ctggatttac cactaccatt ctgaccaata aaaaaaacga catcgccata attaagatta
     2461 agctcatgaa gacgatgttc tctagtaggt aactgaactt ttatcatttt cctttctctt
     2521 tagcttttaa tctaaaatac gttgaacgac taattcctaa attaatccat ggagagttta
     2581 atgaaggcct acctcctcct ttactttttt tcccacatag agattggatg tctgccgaat
     2641 gggtcttttt tataaaatca tcaaatgatg actctgtaaa atttttatta gtccacttag
     2701 ctacactttt ggcaataccc atgacttcgt tctcgtctaa aggtgcggag aactgcaggt
     2761 tgtaggcttt agcgcgttca aagcaagctt gtagccattg gtcatactgc ggccagcctt
     2821 ggtggatagc gcggtaatcc cacttgcggg ttttatcgaa gagggtgcag ttacggccta
     2881 aaccgtagtc cggtaggatt tcgcggtcat tagctgcgcc aaggtcgagg tagtcggcta
     2941 atcagtcgag ggtgtagagc tcaggctgcc aaacggtgat ctgccagtgc aggtggttcg
     3001 gattctcgca aattagccct gaataccccg catcggcgcc caatttttta cgcagcgcat
     3061 tctcaatgac ggctgcatat ttgagggggg cggcgagccc cctgaaccag tcacagaagg
     3121 cacctcgcag aggggacgct gtgagtactg acttaactat gcggtatcag tgcggattgt
     3181 atgaaaagtg cgctattgac ggtgtgaaat gccgcacaga tgcgtaagga gaaaatgcaa
     3241 atgcctacgc tcttccgctt cctcgctcac taactcgctg cgctcggtcg ttcggctgcg
     3301 gcgagcggta tcagctcact cagagacggt aatacggtta tccacagaat caggggataa
     3361 ggccggaaag aacatgtgag caaaaaggca aagccagggc aaaaggccgc gccgctggcg
     3421 tttttccata ggctccgccc ccctgacaag catcacagaa tctgacgctc aaatcagtgg
     3481 tggcgaaacc cgacaggact taaagatacc aggcgtttcc ccctggtggc tccctcgtgc
     3541 gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttat
     3601 ctcattccac gcctgacact cggttccggg taggcagttc gctccaagct ggactgtatg
     3661 cacgaccccc ccgttcagtc cgaccgctgc gccttgtccg gtaactatca tcttgagtcc
     3721 aacccggaaa gacacgactt atcgccactg gctgcggcca ttggtaaggg gtgtacaaga
     3781 gattactgat gcagagttct tgaagttaga accaaactac ggctaaactg gaaggacgaa
     3841 tttggtggca gcggtcttgt acaccctgtt accacggttc agaagttccc caacttactg
     3901 aaccatcgaa aaaccacctc ccaggtggtt ttttcgtttt cagagcaaga gattacgcgt
     3961 agaaaaaagg atctcaagca gatccttttt gattcatgtc ataacccaac ttaccccccc
     4021 acactgaact gatgaaaaaa acagtcaact ctggaatcag tactttaaaa aagcctcaaa
     4081 tgacattttg aggggataca gcggcaaggg ctacaactac acagctgcaa gttacctagt
     4141 tgtaagccta ggcgaagatc aggtatactc gaatcaaggt gatttgctac ctttaagtgc
     4201 agctagaaat tcaggtgatt tgctaccttt aagtgcagct agaaattcag gtgatttgct
     4261 accttaagtg cagctagaaa ttcaggtgat ttgctacctt taagtgcagc tagaaattca
     4321 ggtgatttgc taccttaagt gcagctagaa atttaggtca tttactacct taaagtaata
     4381 cagtaaaaga ggcgactaca cagtcgcctt ttttacgaga aaaaaatgaa attttacact
     4441 atatcaagca gatacattga atacctaaag ggattcgaag acaaagttcc caatagcgaa
     4501 gatcctaact atcagaatcc gaaagctttc attggcattg tgttagagat tcaagggcat
     4561 aagtatctag cccctttaac atccccaaag ccatggcata gcactattaa agaatcatcg
     4621 cctagcttct ttaagctcca tgaaaatggt gtccccgaaa atcagctcgg attgattaat
     4681 ctgaagttca tgattccaat catcgaagcc gaagtatcct tacttgactt gaacggtatg
     4741 ccgaataccg cctacaagag aatgctctat aagcagctcc agtttattcg tgtaaattca
     4801 gacaaaatag ctgagaaatc agagctttta aggaagctcg tcctgcaagg aaaaatgcaa
     4861 ggaacatgta atttttccct gttagaagaa aaataccgag atttcggcaa agaagctaat
     4921 gacacggaag aaggcgagta acgcttcatt gccatcctga atagtgtccg aaaccgagtg
     4981 atcttggaca ctattcaggt ctaatattca aaactcaacg gttcgactca catacatagt
     5041 tcacggtgaa ccatattatg attaatacaa ataccaaaac tcaaataaaa aacctttaaa
     5101 aacaacaaga taaaaaatta acatcacttt tatctttcta gatagatttt tgtgaaggcg
     5161 ttacaacgcc ttcacaccct gagataataa aaacaacaag aagcgattac gaggggtgaa
     5221 aaatgtttga gaaaggcgtg tgtctggttg agaggcttgc gaaagtcgca gcggtaataa
     5281 ctaaccttgt gctctcactt tctatattgg gattggtcta tttcatcttt caaaacttgg
     5341 ccttgaggtg attcatgctg tactggaaaa aaatgccttc actatggata ggaaatagga
     5401 tcgctgaatt cagcgatctt gatacagcaa aagctatagc ggcattaaaa atttatttaa
     5461 cattctgcct gttttgcaaa gagtcagact ctggccacag aacggttgag ttaaccttta
     5521 gcaacctttg cgagatagcg tcattaagcc gttccttggt gaacgaaggc cttaaaattc
     5581 tttatgcaaa agagctgata aaaaacatat cgccgactgc acgaaaaaag atttataccg
     5641 tggatgtgct agaaaatcat aatgatggat ggtgcaagct tccatttaaa ggagttgtcg
     5701 acgaggacaa aaaaataagt gcatttcaat ccatgcacaa cagatatccc tttgagttac
     5761 tagccttaca aacttacatg tatcttcttt acgctcgtga taataacagc gactacacac
     5821 tggctaggaa aaagaccata tgcaaaaaat tgaaatgcaa attacctgaa ctaaacaaag
     5881 caatcaccta ccttatccat ataggtttgc ttgatagagt tgtgaaaaaa tccatagaaa
     5941 acatacctct agagatgttt catgactcgt actatttcta tataaaaaca ggcagtaaga
     6001 gcgctctaac ctacagaagg aaatcagaac cgccagttat tccagaagcc gacttaccat
     6061 tttgattgtt tattccacac caattatcac acggattgaa cataacatcc gttgcccgca
     6121 cctcagaaac gcgctgaacg cgattctgac gcaaataccg caaaaagaga tgttttcccc
     6181 aaggtaaacc gaaaaaatgc gtcaggatcg ttttcagctc gctgcatagc tatgcatgaa
     6241 agtgaatggc gaacggtttg ggcgcatacg gtgtccatga gacacatttt cggacatttt
     6301 cagttcgtga atccctgact cattgctaca tatctgaaca gacaccccta gcagcgcccc
     6361 caggggcatc ctgtaaaaaa acacaccgcg ccgctagcag cacccctaat ataaaatgat
     6421 gttttttatg aaaatagtca gtgccacccc tacaaagcgg tgtcggcgcg gtgttgtagc
     6481 cgcgccgaca ccgctttttt aaatatcaca aagagagcaa gagaaactaa tttgtcataa
     6541 ctctctattt ataaagaaaa atcagcaaaa aattgttttt gcgtggggtg tgggcatttc
     6601 aggcatgaga acagactcgc gtttttccgg agtatcagcg gaattattga ttaagaaaca
     6661 ctcgacgcag agcgaaattt tatgcaacag cggctgcact gcacagggat tatttatcag
     6721 ttgatatgct gtgccatgat acgtcctaaa aaaaacagga caggatgtgc aattgtaata
     6781 ccgtcgcccg caacgtctta caattaccac ctagtcaggg tccacccgac acccggtact
     6841 gacggagaac aacggaaggt gaacaaacag caacaaactg cgctgaatat ggcgcgattt
     6901 atcaaaagcc agagcctgac actgcttgaa aaactggatg ctctggatgc cgacgtacag
     6961 gcggccatgt gtgaacgact gcacgaactc gcagaagaac tccagaacag catccaggtt
     7021 cactttgagg ccgaaaatga aacaggaaca taacgaagct gacgaaggcg gtcacggctt
     7081 gcccgtgagc attttcgtca gcagacaagc ccgtcagggc gcgtcagcgg gttttaacgg
     7141 gtgtcggggc gcagccctga cccagtcacg tagcgatagc ggagtgtata ctgacttaat
     7201 acagcaacta tcgtacatcg cggtcaagtc acgcacaaac aaaaagcccc gcaaaagcag
     7261 ggctgttatc tgatataggt tgtttagtct cacatagcaa aaactgtatg ggtatcagct
     7321 gggctatttt ccgtatctga acatcccaaa atagcttcat ctaaccctcc gaggagggat
     7381 aaaacaaatg ctctagcgtc agcattactc ttgatcgtac gaatcacgtt taactcatat
     7441 ctagagcgat cggcaaaacc acatttttta gctacatgtg ccaacaaatt tgtttctcgg
     7501 atagggtagc gtgagactaa cgcatccaaa tttcgttccg cgatcattgc atcaaatgca
     7561 gcaatttctt tattaacata acattcaata tcaatttctt tattaaactt aatgcggttc
     7621 ttaatatctt gctgttttgg tatggccgac attaagtctg cccttattgt tttttcaact
     7681 gcacggcagc ataagtgatc gcgcttttca tttatggcat caatagcgaa gtcatgaatt
     7741 tccataaata aggcatcaac attatctatc ccattagcct cttttacaaa gtcaatgaca
     7801 aacttaatca ttctaggatg ataataaatc gactcaatag aatgaacgtc gagactaaaa
     7861 atgaagtctt tctttaagtt atcgaccttc gcctgcacct tgttatcatt atcaatgata
     7921 ccaaacgccg atactcgatg aatttcgtca tttagtctca agccttttac cgccaattca
     7981 accaaatcac atttttgttt agatacaact gaaaccatag gaaatatggc cgaatataaa
     8041 tcacggtcaa ggctagacgt aacaccttca acaaatagaa ccttatccct agcgccaagt
     8101 atatctattt tagtttgctc atcaacacca ttgaataatt cgtcataatc ctcaacaaca
     8161 ttgaaatccc agtatatcgg ttctctttgt tcggaaaaat agcagttttt aacgttaacc
     8221 actttcacgt tatcaaaaaa cgcaggcaag cttaactcgt gggtggcaac aaaaaaaaca
     8281 caatctctac gatatttcat taaagatgaa agcagcggag acacaatgct tctgtgcata
     8341 tgtcgctcag gctcatctaa gaaaatagtt gtgttttcat cagcgcaaat aatctcactg
     8401 gcaataatta gtgcactttt ttcaccgtca gaaagcattg atataggata aacatcagga
     8461 ttttcacctt taatattcct tgccataacg ttggcttcat catctagaaa gaactcaata
     8521 gataatttgg catcagcaaa aaccatgttt agcacatcaa cctctgactt tattaactct
     8581 gccggctgat tgttatttga tttaaatgaa tctacgaaac ttaaagccct ggctatcact
     8641 ttgtttttta attttactat aggtatggca accatctgtg agtcataatt aactctgtat
     8701 ctacattgac tgcctttaat ttcattaaaa atccatccct gcgcattttt atactcacta
     8761 ttactgaaat caataatgtc gctgctgaat gtattttttc tatgcgctaa aacatatact
     8821 gatgattgag atcctatttt ttgaaaaaga ttatttaata ggctggattt accactacca
     8881 ttctgaccaa taaaaaaaac gacatcgcca taattaagat taagctcatg aagacgatgt
     8941 tctctagtag gtaactgaac ttttatcatt ttcctttctc tttagctttt aatctaaaat
     9001 acgttgaacg actaattcct aaattaatcc atggagagtt taatgaaggc ctacctcctc
     9061 ctttactttt tttcccacat agagattgga tgtctgccga atgggtcttt tttataaaat
     9121 catcaaatga tgactctgta aaatttttat tagtccactt agctacactt ttggcaatac
     9181 ccatgacttc gttctcgtct aaaggtgcgg agaactgcag gttgtaggct ttagcgcgtt
     9241 caaagcaagc ttgtagccat tggtcatact gcggccagcc ttggtggata gcgcggtaat
     9301 cccacttgcg ggttttatcg aagagggtgc agttacggcc taaaccgtag tccggtagga
     9361 tttcgcggtc attagctgcg ccaaggtcga ggtagtcggc taatcagtcg agggtgtaga
     9421 gctcaggctg ccaaacggtg atctgccagt gcaggtggtt cggattctcg caaattagcc
     9481 ctgaataccc cgcatcggcg cccaattttt tacgcagcgc attctcaatg acggctgcat
     9541 atttgagggg ggcggcgagc cccctgaacc agtcacagaa ggcacctcgc agaggggacg
     9601 ctgtgagtac tgacttaact atgcggtatc agtgcggatt gtatgaaaag tgcgctattg
     9661 acggtgtgaa atgccgcaca gatgcgtaag gagaaaatgc aaatgcctac gctcttccgc
     9721 ttcctcgctc actaactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9781 ctcagagacg gtaatacggt tatccacaga atcaggggat aaggccggaa agaacatgtg
     9841 agcaaaaagg caaagccagg gcaaaaggcc gcgccgctgg cgtttttcca taggctccgc
     9901 cccctgacaa gcatcacaga atctgacgct caaatcagtg gtggcgaaac ccgacaggac
     9961 ttaaagatac caggcgtttc ccctggtggc tccctcgtgc gctctcctgt tcctgccttt
    10021 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttat ctcattccac gcctgacact
    10081 cggttccggg taggcagttc gcccaagctg gactgtatgc acgacccccc cgttcagtcc
    10141 gaccgctgcg ccttgtccgg taactatcat cttgagtcca acccggaaag acacgactta
    10201 tcgccactgg ctgcggccat tggtaagggg tgtacaagag attactgatg cagagttctt
    10261 gaagttagaa ccaaactacg gctaaactgg aaggacgaat ttggtggcag cggtcttgta
    10321 caccctgtta ccacggttca gaagttcccc aacttactga accatcgaaa aaccacctcc
    10381 ccaggtggtt ttttcgtttt cagagcaaga gattacgcgt agaaaaaaag gatctcaagc
    10441 agatcctttt tgattcatgt cataacccaa cttacccccc cccacactga actgatgaaa
    10501 aaaacagtca actctggaat cagtacttta aaaaagcctc aaatgacatt ttgaggggat
    10561 acagcggcaa gggctacaac tacacagctg caagttacct agttgtaagc ctaggcgaag
    10621 atcaggtata ctcgaatcaa ggtgatttgc tacctttaag tgcagctaga aattcaggtg
    10681 atttgctacc tttaagtgca gctagaaatt caggtgattt gctaccttaa gtgcagctag
    10741 aaattcaggt gatttgctac ctttaagtgc agctagaaat tcaggtgatt tgctacctta
    10801 agtgcagcta gaaatttagg tcatttacta ccttaaagta atacagtaaa agaggcgact
    10861 acacagtcgc cttttttacg agaaaaatga aattttacac tatatcaagc agagatacat
    10921 tgaatacc
//
