LOCUS       NZ_CP083891             6055 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Escherichia coli strain NDM1 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP083891
VERSION     NZ_CP083891.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21398202
            Assembly: GCF_020149645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Simner,P., Mostafa,H., Bergman,Y., Ante,M., Tekle,T., Adebayo,A.,
            Beisken,S., Dzintars,K. and Tamma,P.D.
  TITLE     Progressive Development of Cefiderocol Resistance in Escherichia
            coli During Therapy Is Associated with Increased blaNDM-5 Copy
            Number and Gene Expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Simner,P., Mostafa,H., Bergman,Y., Ante,M., Tekle,T., Adebayo,A.,
            Beisken,S., Dzintars,K. and Tamma,P.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) BioIT, Ares-Genetics, Karl-Farkas-Gasse 18,
            Vienna 1180, Austria
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Canu v. JAN-2021
            Assembly Name                     :: Isolate1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 67x
            Sequencing Technology :: Oxford Nanopore MiniION + Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 01:54:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,150
            CDSs (total)                      :: 5,024
            Genes (coding)                    :: 4,771
            CDSs (with protein)               :: 4,771
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 1 of 253
            Pseudo Genes (frameshifted)       :: 124 of 253
            Pseudo Genes (incomplete)         :: 145 of 253
            Pseudo Genes (internal stop)      :: 42 of 253
            Pseudo Genes (multiple problems)  :: 55 of 253
            Pseudo Genes (short protein)      :: 4 of 253
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083891.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NDM1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: Baltimore"
                     /lat_lon="39.296 N 76.591 W"
                     /collection_date="2020"
                     /collected_by="Johns Hopkins University"
                     /genotype="ST90"
     gene            74..397
                     /locus_tag="LCE25_RS25265"
                     /old_locus_tag="LCE25_25265"
     CDS             74..397
                     /locus_tag="LCE25_RS25265"
                     /old_locus_tag="LCE25_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            606..>1046
                     /locus_tag="LCE25_RS25670"
                     /pseudo=""
     CDS             606..>1046
                     /locus_tag="LCE25_RS25670"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1075..1635
                     /locus_tag="LCE25_RS25275"
                     /old_locus_tag="LCE25_25275"
     CDS             1075..1635
                     /locus_tag="LCE25_RS25275"
                     /old_locus_tag="LCE25_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067486.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGMKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1642..1875
                     /locus_tag="LCE25_RS25280"
                     /old_locus_tag="LCE25_25280"
     CDS             1642..1875
                     /locus_tag="LCE25_RS25280"
                     /old_locus_tag="LCE25_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3193..3384)
                     /locus_tag="LCE25_RS25285"
                     /old_locus_tag="LCE25_25285"
     CDS             complement(3193..3384)
                     /locus_tag="LCE25_RS25285"
                     /old_locus_tag="LCE25_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3448..3771
                     /locus_tag="LCE25_RS25290"
                     /old_locus_tag="LCE25_25290"
     CDS             3448..3771
                     /locus_tag="LCE25_RS25290"
                     /old_locus_tag="LCE25_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3761..4450
                     /locus_tag="LCE25_RS25295"
                     /old_locus_tag="LCE25_25295"
     CDS             3761..4450
                     /locus_tag="LCE25_RS25295"
                     /old_locus_tag="LCE25_25295"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_224446629.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLRGNPEEVRELID
                     STPFSKKYTSGVLSFAEKELPPGERERVMTSFERVLMPGLEKDQYSILWVEHQDKGRLE
                     LNFVIPNMELQSGKRLQPYYDRADRPRINAWQTLVNHHYGLRDPNAPENRRTLVTPNNL
                     PKAKQEAAEAITRGLEALYHAGAIKTRQDVTEALTAAGFEVVRTTKSSISIADPEGAES
                     QAKGGAV"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4447..5007
                     /locus_tag="LCE25_RS25300"
                     /old_locus_tag="LCE25_25300"
     CDS             4447..5007
                     /locus_tag="LCE25_RS25300"
                     /old_locus_tag="LCE25_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067487.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            5014..5247
                     /locus_tag="LCE25_RS25305"
                     /old_locus_tag="LCE25_25305"
     CDS             5014..5247
                     /locus_tag="LCE25_RS25305"
                     /old_locus_tag="LCE25_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5521..5847)
                     /locus_tag="LCE25_RS25310"
                     /old_locus_tag="LCE25_25310"
     CDS             complement(5521..5847)
                     /locus_tag="LCE25_RS25310"
                     /old_locus_tag="LCE25_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_112029608.1"
                     /translation="MKLSAPVAELSRVRSPSSQLPQPQTVFANTQASAKKGFFFGCLGG
                     CAAEKFLKNTEGSCKLNELVSVAANNRLHRNIEEVENLNEPEEARLMAGFFRSTLPDER
                     SQVK"
                     /name="hypothetical protein"
     Typing          3029..3112
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.059"
                     /coverage="95.0"
                     /evalue="8.83e-18"
     Typing          388..1958
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.817"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4417..5330
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.032"
                     /coverage="100.0"
                     /evalue="7.72e-147"
ORIGIN
        1 tgtttggtca tggtcttaag gctccttgta tgggggattt gttaggggaa tgataccgat
       61 gaaacgcgag aagatgctca cgatacgggt taccgatgag gagcacgccc ggttactgga
      121 acgctgcgag ggtaaacgac tggcggaatg gatgcggcgg gtctgtctgg gggagccggt
      181 tgcacgcaca ggaaaactgc cgacactggc accgccattg ctgcggcatc tggctgccat
      241 cgggaacaac ctgaaccaga cagcccggaa ggtgaacagc ggccagtggt cttcgattga
      301 ccgggttcat gtggtggccg cactgatggc catcgagggt gaattgcgcc agttgcggca
      361 ggctgtcagg gaacaggggg tacgggatga tagttaaatt tcatgccagg gggaaaggcg
      421 gtggaagtgg cccggtcgat tacctgctgg ggcgggagcg taaccgcgaa ggcgcaacgg
      481 tgctccgggg taacccggaa gaaatccggg aactcattga tgccacgcca ttttcgaaga
      541 aatacacgtc aggcgttctg tcgttcgcgg agaaggagct accaccgggt gaacgcgaaa
      601 gagtgatgac gagctttgag cgtgttctta tgcccggtct cgaaaaagac cagtacagca
      661 ttctctgggt ggaacaccag gataaggggc ggctcgaact gaattttgtc atcccgaaca
      721 tggagctaca gagcggtaag cgcctccagc cgtactacga ccgtgcggac aggccgagga
      781 taaatgcctg gcagacgctg gttaatcatc attacgggct gcgtgacccg aacgcaccgg
      841 agaacagacg gacgcttgtc acgccaaata atctgccaaa agcgaaacag gaggccgcag
      901 aagcgattac acggggtctt gaggctcttt accatgccgg agcaataaaa acgcgtcagg
      961 atgtcacaga ggcgcttaca gcggcaggtt ttgaggtggt gaggacaacc aaaagcagca
     1021 tcagtattgc cgacccggag ggggggcgga atctcaggct aaagggggcg ctgtatgagc
     1081 aatcttttac aaatgggaac agacttcgag aagaaactga aagagcgagc cgcttctacc
     1141 gagaacatgc tgaacagcga gttcaggaag ctcgaagagt ctgtagacaa ggcattgagc
     1201 ttaaacaggc agaaaatcag agacgctatc agcgaacata caacatccgt gaaaaagcaa
     1261 ctggatacgc tgagcaccac cgtgagcatg cagttcagca ccacagaggc cgaactgtcc
     1321 cggcagcaga aaaaactgct ctggcgggtg atcaaagggc ggatactgtt tccggcgctg
     1381 accgcgctca gcgtcacggg cgggattttc ctggggtgct gggggctgat ggagtggcag
     1441 gaaagcaaga ttgcgaaaaa tatcctgact atcagagagc aggaaaacac gctggcgaaa
     1501 ctcgaggcaa aaacctgggg ggtgacgttc gtcaatggcg agaacgggaa atttctggtg
     1561 ttgccggacg ggatgaaagg ggaaaacacc tggacggtgg gcgacaagaa cgccgtgagg
     1621 ctggtcaggg agtaaacgag tatgacggaa ctggaagggc atttgctgaa cgccttagag
     1681 catttgcaac aggactatat gcggcggctg aacgagtggg agagcgcctt cgcggaattg
     1741 cagaagatgc acgaggttac gcaacggaac aacgcgatac tgaacgagcg cgtcgtgata
     1801 ttgagtcagc aggtgcagag attagccggg caagtggatc gcttgagtcg gttatttatc
     1861 gcgaacaaca gctaagggat gaacaggagc gtgcccggta cagacatgag gtacaggaac
     1921 gcgcggcacg ggttcggagt gggccatcaa tggagatgtg accggggaag tgacgaaatc
     1981 ttgccatttt gacccgttcg acggctgggg cgagcgaaac aaatcgtgtg ccgtatttgt
     2041 gcatagctat gcaacatcct gaaaacgcat ttcacgcgtt tttggggatt cagccagggg
     2101 aaaacatggt ttttttactt tttgagcgta aagacgtgaa aaatctcgtt attgcacttg
     2161 cgatctttcg tcggcgggcg tagaacgaaa acagctcgcc aggaaggcga gctgtttcgg
     2221 gttcattgag gtctctgacc tcctcaatgt tcctgtggaa actgttgaga tccacaacag
     2281 aaaccagttc attcagtttg catgagcctt ctgtattctt caagaatttt tcaacagcac
     2341 agtctcccag gaaccccgaa aaaaacctgt ttttgcagat acttgcgtat ttgtgagaat
     2401 cgtttgcggt tgggggagct gtgacgacgg cgaccgcacg cgtgagagtt ccgctaccgg
     2461 cgcagagagt ttcatcgaca gcggacacct gatgagttgc cacaaaaacc gtatgtagcg
     2521 cggctagatt ggttgagtta gaccttggcg gcagttcccg gtatgtggtg gcgtgacggg
     2581 tgagatggtg cggcgaagac gcatacgtca cgcaggcgcg ggttcttcca ctgacgcgcc
     2641 ccgtgatcga tggccttggc tccgtccggc atatcgagag cggcaaacta cccgtccctt
     2701 cactgggagg ggtgagttgt tgccgttccc tccgaccttc caccatccag catatcgcac
     2761 gatcccatgt gcaccgctac tcctgataac tccagtgcaa aaaaaaaaaa cggatctgca
     2821 tcacaacgag agtgatccca ccgctcgccg cagccccagc gaccgaacgt agtgagcgag
     2881 tgggcgagga agcggaagag tcacagaggc aagcattgtg cacttacgcg cctgtgccag
     2941 tgtccgcgtg tgtgttgttt atcgggcgcg ctgtttttac gcatctgtgc ggctttttac
     3001 tcctgacatg gtgcacttcc catccactct gcaccgatgc aaaatgaggt aagccagtat
     3061 acacatcgct atcgctctgt gaactgggtc agggctgcgc cccgacaccc gcactgacgc
     3121 gccctggcgg gcttgtctgc tcccggcatc cgctcacagg caagctgtga acgtctccgg
     3181 gagctgcatg tttcagttgc tttcaccgtc atcgccgaag cgtgcgaggc aactgcggta
     3241 gagttcgtcg gcgtggtcgt gaagtgattc gcagatgtct gcctgttcgt ccgcgtccag
     3301 atcgttgagt ttttccagca gggttagcgt ctggcttctg ataaagcgag ccatgttaag
     3361 ggctgttttt tcctgtttgg tcatggtctt aaggctcctt gtatggggga tttgttaggg
     3421 ggaatgatac cgatgaaacg cgagaagatg ctcacgatac gggttaccga tgaggagcac
     3481 gcccggttac tggaacgctg cgagggtaaa cgactggcgg aatggatgcg gcgggtctgt
     3541 ctgggggagc cggttgcacg cacaggaaaa ctgccgacac tggcaccgcc attgctgcgg
     3601 catctggctg ccatcgggaa caacctgaac cagacagccc ggaaggtgaa cagcggccag
     3661 tggtcttcga ttgaccgggt tcatgtggtg gccgcactga tggccatcga gggtgaattg
     3721 cgccagttgc ggcaggctgt cagggaacag ggggtacggg atgatagtta aatttcatgc
     3781 cagggggaaa ggcggtggaa gtggcccggt cgattacctg ctggggcggg agcgtaaccg
     3841 cgaaggcgca acggtgctcc ggggtaaccc ggaagaagtg cgggaactca ttgattccac
     3901 gccattttcg aagaaataca cgtcaggcgt tctgtcgttc gcggagaagg agctaccacc
     3961 gggtgaacgc gaaagagtga tgacgagctt tgagcgtgtt cttatgcccg gtctcgaaaa
     4021 agaccagtac agcattctct gggtggaaca ccaggataag gggcggctcg aactgaattt
     4081 tgtcatcccg aacatggagc tacagagcgg taagcgcctc cagccgtact acgaccgtgc
     4141 ggacaggccg aggataaatg cctggcagac gctggttaat catcattacg ggctgcgtga
     4201 cccgaacgca ccggagaaca gacggacgct tgtcacgcca aataatctgc caaaagcgaa
     4261 acaggaggcc gcagaagcga ttacacgggg tcttgaggct ctttaccatg ccggagcaat
     4321 aaaaacgcgt caggatgtca cagaggcgct tacagcggca ggttttgagg tggtgaggac
     4381 aaccaaaagc agcatcagta ttgccgaccc ggagggggcg gaatctcagg ctaaaggggg
     4441 cgctgtatga gcaatctttt acaaatggga acagacttcg agaagaaact gaaagagcga
     4501 gccgcttcta ccgagaacat gctgaacagc gagttcagga agctcgaaga gtctgtagac
     4561 aaggcattga gcttaaacag gcagaaaatc agagacgcta tcagcgaaca tacaacatcc
     4621 gtgaaaaagc aactggatac gctgagcacc accgtgagca tgcagttcag caccacagag
     4681 gccgaactgt cccggcagca gaaaaaactg ctctggcggg tgatcaaagg gcggatactg
     4741 tttccggcgc tgaccgcgct cagcgtcacg ggcgggattt tcctggggtg ctgggggctg
     4801 atggagtggc aggaaagcaa gattgcgaaa aatatcctga ctatcagaga gcaggaaaac
     4861 acgctggcga aactcgaggc aaaaacctgg ggggtgacgt tcgtcaatgg cgagaacggg
     4921 aaatttctgg tgctgccgga cggggtgaaa ggggaaaaca cctggacggt gggcgacaag
     4981 aacgccgtga ggctggtcag ggagtaaacg agtatgacgg aactggaagg gcatttgctg
     5041 aacgccttag agcatttgca acaggactat atgcggcggc tgaacgagtg ggagagcgcc
     5101 ttcgcggaat tgcagaagat gcacgaggtt acgcaacgga acaacgcgat actgaacgag
     5161 cgcgtcgtga tattgagtca gcaggtgcag agattagccg ggcaagtgga tcgcttgagt
     5221 cggttattta tcgcgaacaa cagctaaggg atgaacagga gcgtgcccgg tacagacatg
     5281 aggtacagga acgcgcggcg cgggttcgga gtgggccatc aatggagatg tgaccgggga
     5341 agtgaggaaa ccttgccatt ttgacccatt cgacggctgg ggcgagcgaa acaaatcgtg
     5401 tgccgtattt gtgcatagct atgcaatatc ctgaaaacgc atttcacgcg tttttgggga
     5461 ttcagccagg ggaaaacatg gtttttttgc tttttgagcg taaagacgtg aaaaatctcg
     5521 ttatttcact tgcgatcttt cgtcggggag cgtagaacga aaaaagcccg ccattaggcg
     5581 agcttcttcg ggttcattga ggttttcgac ctcctcaatg ttcctgtgga gtctgttgtt
     5641 tgcggcgaca gaaaccagtt cattcagttt gcatgaacct tctgtattct tcaagaattt
     5701 ttccgccgca cagcctccca gacacccgaa aaaaaatcct ttttttgcag atgcttgcgt
     5761 gtttgcaaag accgtttgcg gttgggggag ctgtgacgac ggcgaccgca cgcgtgagag
     5821 ttccgctacc ggcgcagaga gtttcatcga cagcggacac ctgatgagtt gccacaaaaa
     5881 ccgtatgtag cgcggctaga ttggttgagt tagaccttgg cggcagttcc cggtatgtgg
     5941 tggcgtgacg ggtgagatgg tgcggcgaag acgcatacgt cacgcaggcg cgggttcttc
     6001 cactgacgcg ccccgtgatc gatggccttg gctcatatcg cacgatccca tgtgc
//
