LOCUS       NZ_CP083868             6275 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Escherichia coli strain NDM6 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP083868
VERSION     NZ_CP083868.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21398206
            Assembly: GCF_020149705.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Simner,P., Mostafa,H., Bergman,Y., Ante,M., Tekle,T., Adebayo,A.,
            Beisken,S., Dzintars,K. and Tamma,P.D.
  TITLE     Progressive Development of Cefiderocol Resistance in Escherichia
            coli During Therapy Is Associated with Increased blaNDM-5 Copy
            Number and Gene Expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Simner,P., Mostafa,H., Bergman,Y., Ante,M., Tekle,T., Adebayo,A.,
            Beisken,S., Dzintars,K. and Tamma,P.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) BioIT, Ares-Genetics, Karl-Farkas-Gasse 18,
            Vienna 1180, Austria
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Canu v. JAN-2021
            Assembly Name                     :: Isolate6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 234x
            Sequencing Technology :: Oxford Nanopore MiniION + Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 01:44:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,910
            CDSs (total)                      :: 4,787
            Genes (coding)                    :: 4,426
            CDSs (with protein)               :: 4,426
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 190 of 361
            Pseudo Genes (incomplete)         :: 192 of 361
            Pseudo Genes (internal stop)      :: 56 of 361
            Pseudo Genes (multiple problems)  :: 76 of 361
            Pseudo Genes (short protein)      :: 4 of 361
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083868.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NDM6"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Baltimore"
                     /lat_lon="39.296 N 76.591 W"
                     /collection_date="2020"
                     /collected_by="Johns Hopkins University"
                     /genotype="ST167"
     gene            49..488
                     /locus_tag="LCE24_RS01315"
                     /old_locus_tag="LCE24_01315"
                     /pseudo=""
     CDS             49..488
                     /locus_tag="LCE24_RS01315"
                     /old_locus_tag="LCE24_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /name="entry exclusion protein 1"
     gene            complement(1228..1377)
                     /locus_tag="LCE24_RS01320"
                     /old_locus_tag="LCE24_01320"
     CDS             complement(1228..1377)
                     /locus_tag="LCE24_RS01320"
                     /old_locus_tag="LCE24_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1942..2103
                     /locus_tag="LCE24_RS24480"
     CDS             1942..2103
                     /locus_tag="LCE24_RS24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_318010574.1"
                     /translation="MYARTPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
                     /name="replication initiation protein"
     gene            2562..3470
                     /locus_tag="LCE24_RS01325"
                     /old_locus_tag="LCE24_01325"
                     /pseudo=""
     CDS             2562..3470
                     /locus_tag="LCE24_RS01325"
                     /old_locus_tag="LCE24_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519741.1"
                     /GO_function="GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]; GO:0016740 - transferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]; GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /name="dihydropteroate synthase"
     gene            3575..4333
                     /locus_tag="LCE24_RS01330"
                     /old_locus_tag="LCE24_01330"
     CDS             3575..4333
                     /locus_tag="LCE24_RS01330"
                     /old_locus_tag="LCE24_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_001145207.1"
                     /translation="MPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLKG
                     RGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSVDQ
                     CPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRLDQERTDM
                     VVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDEAERA
                     FAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
                     /name="APH(3'') family aminoglycoside O-phosphotransferase"
     gene            4333..5165
                     /locus_tag="LCE24_RS01335"
                     /old_locus_tag="LCE24_01335"
                     /pseudo=""
     CDS             4333..5165
                     /locus_tag="LCE24_RS01335"
                     /old_locus_tag="LCE24_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /name="APH(6)-I family aminoglycoside O-phosphotransferase"
     gene            complement(<5271..5672)
                     /locus_tag="LCE24_RS01340"
                     /old_locus_tag="LCE24_01340"
                     /pseudo=""
     CDS             complement(<5271..5672)
                     /locus_tag="LCE24_RS01340"
                     /old_locus_tag="LCE24_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /name="transposase zinc-binding domain-containing protein"
     gene            complement(5716..6105)
                     /locus_tag="LCE24_RS01345"
                     /old_locus_tag="LCE24_01345"
     CDS             complement(5716..6105)
                     /locus_tag="LCE24_RS01345"
                     /old_locus_tag="LCE24_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     ARG             3549..4330
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="97.75"
     ARG             2691..3121
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="99.22"
                     /coverage="52.77"
     ARG             2563..2693
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="15.87"
     ARG             4521..5068
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="65.47"
     ARG             4334..4551
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.01"
                     /coverage="25.9"
     ARG             3576..4333
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.21"
                     /coverage="94.38"
     Typing          complement(1413..1585)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="1.99e-82"
     Typing          1509..2112
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.961"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgttctgct gttacaatac ggtacacaca ggagacactg tcacttttat ggcatggcac
       61 acagttcaag agacactgaa attaacagga aagtcgcgct cgcaattata tcgtgacatg
      121 gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc gccgggagtt cgagaccagt
      181 gaactgattc gggcctatgg cgagctgcgc acttctgaga caccaaaaag gcacaaggag
      241 ggacactcca aatctgtcgt tagccaggcg gaaaatgctc agctagacgt cattcagcag
      301 aaactggacg ctctgcagca gactgttacc ctaatgcttg aagacaagat ggcgcgggaa
      361 gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac aggccgaaat tagccgtctg
      421 gctgacgcgc tggcacagga aaaaaagcgg gattctggtc gcggctgttt cgctccggtt
      481 cagagtaaca gtgcaggtga ctctgattta ccataacatt cgttgcgtgc accatctcag
      541 acgccctcag ggcgtttctg acgggaaacc ggtaaaacag gcaacaaacc acccgaaaac
      601 ccgccagaaa cgcgcaggct ctgtttttac ggcatgcatg agtatgcgca caagtgcata
      661 gcgcggaaat tcacaccatt cagccgggta agtcctgttt ccggcgttct tcccatccga
      721 aaacggtgaa cggggacgca atttattgcg tggccgtgaa gcgttgccgg cgcagccgga
      781 aaacccgctg tcagcacgtt caggaagggg gtgatgtaca aatggcacag aggatgaaag
      841 tgacgtatga gctgaaggaa tgatgtaaaa gtgatgtata agtgatgtaa aactgatgta
      901 tgaactgaag gagcgatgta attctgtagt caggaagaat aacccgttca tgcaggcatg
      961 aacgatacag tgaggaacgc tttttttgtc acgatctccc ttaacctgcg taaatcaccc
     1021 gccatggttg attatttttc aacctcaaaa tggtcaacac actaacgcag ccgcgatttt
     1081 catcgcaact gcatgattaa aagaaaaaaa ggaatatttc gttttcacga gtaatacccg
     1141 ggtattaaaa atccactgta attacatgaa aataaagaaa taaaggtaat accggagtat
     1201 taccacgtac gcgggcaagc gaaaaagcta taacgcttcc cggccgacag gcgacccaaa
     1261 atccacaggg gcatgcaggt tctcttcagt aacctgcgcc attaaatcaa aaaggtcata
     1321 ttcgccggga agctgcgtat cagtcgaatc cagttgttca tcatattcag atggcatggg
     1381 gtgatatcca gtagctgaga ggttgttagc gggtttcggg gcgcagccct gaccagtcat
     1441 gtagcgctag cggagtgtat actggcttac tatgttgcca cgattgcgaa aatcagtgaa
     1501 gtgcttcatg tggcaggaga aaaaaggcag caccggcgcg tcagcagaat atgcgataca
     1561 ggagatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag
     1621 cggtaccggc ttacggacgg ggacggaaat ttctggaaga taccaggaag acacttagca
     1681 aagaagagat aaggccacgg cgaagccgtt tttccatagg ctccgccccc tgacaagcat
     1741 cgcaaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagatcccag
     1801 gcgtttcccc ctggtagctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     1861 gtcattccgc tgttatggcc gcggtttctc attccacgcc tgacactcag ttccgtgaag
     1921 agcagttcgc tccaagctgg actgtatgca cgaacccccg ttcagtccga ccgctgcgcc
     1981 ttgtccggta actatcaact tgagtccaac ccggaaagac acgacaaaac gccactggca
     2041 gcagccatta gtaacaggag tgcacgagaa gatagattaa gaggtcttga agtagaggcc
     2101 tgactgcggc tacactgaaa gaacagtttt ggtaatggcg gtctcgtgaa ccctgttacc
     2161 gggttaagca gttccccaac tgacttaacc ttcgaacaaa ccgcctcccc aggcggtttt
     2221 ttcgtttaca gagcaagaga ttacgattca ttgaaaagga tctcaagaag atcctttgta
     2281 gattactatc aattaatctt ccgaacatga caccagtcac tgttgattac ggttaaaccg
     2341 ctccgtaaca ggtttccctc gtattggtga cttgcagcac caaagaagca aaaaagataa
     2401 atatatctac ttattgagag atgatatatt tatttccaaa atgctacaga tacatcatca
     2461 tgaaacggat caccgcaaac aggttactcg cctgggattc cctttcgacc cgagcatccg
     2521 tatgagactc atgctcgatt attattatta tagaagcccc catgaataaa tcgctcatca
     2581 ttttcggcat cgtcaacata acctcggaca gtttctccga tggaggccgg tatctggcgc
     2641 cagacgcagc cattgcgcag gcgcgtaagc tgatggccga ggggcagatg tgatcgacct
     2701 cggtccggca tccagcaatc ccgacgccgc gcctgtttcg tccgacacag aaatcgcgcg
     2761 tatcgcgccg gtgctggacg cgctcaaggc agatggcatt cccgtctcgc tcgacagtta
     2821 tcaacccgcg acgcaagcct atgccttgtc gcgtggtgtg gcctatctca atgatattcg
     2881 cggttttcca gacgctgcgt tctatccgca attggcgaaa tcatctgcca aactcgtcgt
     2941 tatgcattcg gtgcaagacg ggcaggcaga tcggcgcgag gcacccgctg gcgacatcat
     3001 ggatcacatt gcggcgttct ttgacgcgcg catcgcggcg ctgacgggtg ccggtatcaa
     3061 acgcaaccgc cttgtccgca cccgctggcg acatcatgga tcacattgcg gcgttctttg
     3121 acgcgcgcat cgcggcgctg acgggtgccg gtatcaaacg caaccgcctt gtccttgatc
     3181 ccggcatggg gtttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt
     3241 cgatgaattg cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt
     3301 tctgcgcgcg ctcacaggcc gtggtcgggg atgtcggggc cgcgacactc gctgcagagc
     3361 ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc cccttgcgcg
     3421 acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa ctgcacattc
     3481 gggatatttc tctatattcg cgcttcatca gaaaactgaa ggaacctcca ttgaatcgaa
     3541 ctaatatttt tttggtgaat cgcattctga ctggttgcct gtcagaggcg gagaatctgg
     3601 tgattttgtt tttcgacgtg gtgacgggca tgccttcgcg aaaatcgcac ctgcttcccg
     3661 ccgcggtgag ctcgctggag agcgtgaccg cctcatttgg ctcaaaggtc gaggtgtggc
     3721 ttgccccgag gtcatcaact ggcaggagga acaggagggt gcatgcttgg tgataacggc
     3781 aattccggga gtaccggcgg ctgatctgtc tggagcggat ttgctcaaag cgtggccgtc
     3841 aatggggcag caacttggcg ctgttcacag cctatcggtt gatcaatgtc cgtttgagcg
     3901 caggctgtcg cgaatgttcg gacgcgccgt tgatgtggtg tcccgcaatg ccgtcaatcc
     3961 cgacttctta ccggacgagg acaagagtac gccgcagctc gatcttttgg ctcgtgtcga
     4021 acgagagcta ccggtgcggc tcgaccaaga gcgcaccgat atggttgttt gccatggtga
     4081 tccctgcatg ccgaacttca tggtggaccc taaaactctt caatgcacgg gtctgatcga
     4141 ccttgggcgg ctcggaacag cagatcgcta tgccgatttg gcactcatga ttgctaacgc
     4201 cgaagagaac tgggcagcgc cagatgaagc agagcgcgcc ttcgctgtcc tattcaatgt
     4261 attggggatc gaagcccccg accgcgaacg ccttgccttc tatctgcgat tggaccctct
     4321 gacttggggt tgatgttcat gccgcctgtt tttcctgctc attggcacgt ttcgcaacct
     4381 gttctcattg cggacacctt ttccagcctc gtttggaaag tttcattgcc agacgggact
     4441 cctgcaatcg tcaagggatt gaaacctata gaagacattg ctgatgaact gcgcggggcc
     4501 gactatctgg tatggcgcaa tgggaggagc agtccggttg ctcggtcgtg agaacaatct
     4561 gatgttgctc gaatatgccg gggagcgaat gctctctcac atcgttgccg agcacggcga
     4621 ctaccaggcg accgaaattg cagcggaact aatggcgaag ctgtatgccg catctgagga
     4681 acccctgcct tctgcccttc tcccgatccg ggatcgcttt gcagctttgt ttcagcgggc
     4741 gcgcgatgat caaaacgcag gttgtcaaac tgactacgtc cacgcggcga ttatagccga
     4801 tcaaatgatg agcaatgcct cggaactgcg tgggctacat ggcgatctgc atcatgaaaa
     4861 catcatgttc tccagtcgcg gctggctggt gatagatccc gtcggtctgg tcggtgaagt
     4921 gggctttggc gccgccaata tgttctacga tccggctgac agagacgacc tttgtctcga
     4981 tcctagacgc attgcacaga tggcggacgc attctctcgt gcgctggacg tcgatccgcg
     5041 tcgcctgctc gaccaggcgt acgcttaggt gcctttccgc agcttggaac gcggatggag
     5101 aagaggagca acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat
     5161 actagatatc aagcgacttc tcctatcccc gggaacacat caatctcacc ggagaatatc
     5221 gctggccaaa gccttagcgt aggattccgc cccttcccgc aaacgacccc aaacaggaaa
     5281 cgcagctgaa acgggaagct caacacccac tgacgcatgg gttgttcagg cagtacttca
     5341 tcaaccagca aggcggcact ttcggccatc cgccgcgccc acagctcggg cagaaaccgc
     5401 gacgcttaca gctgaaagcg accaggtgct cggcgtggca agactcgcag cgaacccgta
     5461 gaaagccatg ctccagccgc ccgcattgga gaaattcttc aaattcccgt tgcacatagc
     5521 ccggcaattc ctttccctgc tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa
     5581 cgatctgata gaagggtttg ctcgggtcgg tggctctggt aacgaccagt atcccgatcc
     5641 cggctggccg tcctggccgc cacatgaggc atgttccgcg tccttgcaat actgtgttta
     5701 catacagtct atcgcttagc ggaaagttct tttaccctca gccgaaatgc ctgccgttgc
     5761 tagacattgc cagccagtgc ccgtcactcc cactcttaca ttgttcgcag tatctaggta
     5821 catgttattg cgtgcacctt catgatgtat aatgttatta atgacagcag aaagatcgac
     5881 agaattaata ttcttcgtac ctgggacagt tatcgggtgt cggccagtga tgagaccaaa
     5941 ggatgcagta taagatgaac cactactatt atcatggcca ccattaccac caccgttaaa
     6001 actacctccc gaacttccag agccgctatg accatcattg taggggtcat ttggtcccat
     6061 gtgaccaaaa ccaccgacag taccgctatt taactctgta ctcataaaat tatcctcttt
     6121 gacttttaaa aacagcaaaa actgtattta taacaatgtg tttatataca gtattcgaaa
     6181 tactacgccc gtcaaaacca caccgtcaac aggttttttt gtgtattttt cacgcctcac
     6241 gggcagcctc agaccttttg tgttaaaaca gtaat
//
