LOCUS       NZ_CP083866             3596 bp    DNA     circular CON 06-APR-2024
DEFINITION  Enterobacter kobei strain 11743-yvys plasmid p11743_4, complete
            sequence.
ACCESSION   NZ_CP083866
VERSION     NZ_CP083866.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21379547
            Assembly: GCF_023333675.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Liao,W., Cui,Y., Quan,J., Zhao,D., Han,X., Shi,Q., Wang,Q.,
            Jiang,Y., Du,X., Li,X. and Yu,Y.
  TITLE     High prevalence of colistin resistance and mcr-9/10 genes in
            Enterobacter spp. in a tertiary hospital over a decade
  JOURNAL   Int J Antimicrob Agents 59 (5), 106573 (2022)
   PUBMED   35307563
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Liao,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023333675.1-RS_2024_04_06
            Annotation Date                   :: 04/06/2024 07:15:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,381
            CDSs (total)                      :: 5,264
            Genes (coding)                    :: 5,129
            CDSs (with protein)               :: 5,129
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 39 of 135
            Pseudo Genes (incomplete)         :: 101 of 135
            Pseudo Genes (internal stop)      :: 17 of 135
            Pseudo Genes (multiple problems)  :: 18 of 135
            Pseudo Genes (short protein)      :: 1 of 135
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083866.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="11743-yvys"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:208224"
                     /plasmid="p11743_4"
                     /geo_loc_name="China"
                     /lat_lon="30.15 N 120.10 E"
                     /collection_date="2011-09"
                     /collected_by="Yushan Cui"
     gene            join(2974..3596,1..79)
                     /locus_tag="LCD49_RS26875"
                     /old_locus_tag="LCD49_26940"
     CDS             join(2974..3596,1..79)
                     /locus_tag="LCD49_RS26875"
                     /old_locus_tag="LCD49_26940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249050435.1"
                     /translation="MTISKKNSELRERFKEYASNKNIFDLADLRYEILKIYYDFKLKND
                     MNEQERKSQDSRRKAHLTALKKRIKREIVSKIVIDLVKYYNIEKTTFHFFSHICTEILE
                     RNVDNRYILNNFSNMILDEKKELTKLSESRNASSKMILENSYNELVSMSHIKDKLFRNN
                     NFKTAYLKCYACANEEFSRFKVFAFPDNFETLDFLFEEERIKKEEKEISKIMIEQVEEE
                     QKIQPNKKRRL"
                     /name="hypothetical protein"
     gene            450..1574
                     /locus_tag="LCD49_RS26880"
                     /old_locus_tag="LCD49_26945"
     CDS             450..1574
                     /locus_tag="LCD49_RS26880"
                     /old_locus_tag="LCD49_26945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224331409.1"
                     /translation="MEFKIKKMTVKDSREIKKRFEDCIKLAEEKGLQVGSVLNMEMQDR
                     LKEINLKVKKETAEQYRKLFNYKLSVNAEDIIQHLLECNTRNSFDKTRTAFRFCICEKI
                     QELRNEADRARKQKNFDLMKEKTLEAYKLFFFFERDFLSENRVTWNDISYRKIDSKSKK
                     KTMNSVSSIKAIFEDLKQKPELFDKYSLILAISSLTGCRPAEIQKGVSLEVSPPLLNIC
                     ISGAKVGEDRGQEKRILKFNIKEFAQNEQFNLIMSKIKQNNKFEYKCSKQEYDSLRQYL
                     YINHPGFSLYTLRHRAASELKKDGFSEEMIAAFLGHRVTRSLENYGYARSGKGGPKVAG
                     VEHSNAIKSNTRRYKSKTTPGGGNPSPTRKRALK"
                     /name="hypothetical protein"
     gene            2660..2908
                     /locus_tag="LCD49_RS26885"
                     /old_locus_tag="LCD49_26950"
     CDS             2660..2908
                     /locus_tag="LCD49_RS26885"
                     /old_locus_tag="LCD49_26950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249050434.1"
                     /translation="MEIEKLIDRYFFALEKKIIAEIYNKQTREDLEKFIFKCNQEQLEF
                     ERRFNIICSTEYYEKQKLIFSINYISAEFEQREKLNY"
                     /name="hypothetical protein"
ORIGIN
        1 aaaagaaata tctaaaataa tgattgaaca agttgaagaa gaacaaaaaa tacagccaaa
       61 taaaaaaaga agactataat ttttttgcgg cgaatcgtga gaaaaataaa attttaattt
      121 tgttttttga gcgattgatc tgtgtgtaag ttgatgaaaa atctagaaaa atctgcgata
      181 tattttttct tttgacttgc cattattgtt atttttgtta taacaataat ggggggttgt
      241 cttttacttg aaaaatgagc gtcagcgaaa ttttgaaagg aaaagtgtgc gaagagcaca
      301 aaaccctttt gctttttttc gtaatgacat tttaaaaata tttcattttg agcgaagcga
      361 aatttgaaaa tttttaattt ggaatgaaga aaaaaggcaa acctgcggag taaaagtaaa
      421 acaaaaaaac tataaaaaat gaggtggcta tggaatttaa aataaagaaa atgactgtta
      481 aagacagcag agaaattaaa aaaagatttg aagattgtat taaattagct gaagaaaaag
      541 ggttacaggt tggctctgtt ttaaatatgg aaatgcaaga tagattgaaa gaaattaatt
      601 taaaagtaaa aaaagaaaca gcagaacaat atagaaaatt gtttaattat aaattgtctg
      661 tgaatgcaga agatattatt caacacttgt tagaatgcaa cacaagaaac agttttgata
      721 aaacaagaac agcatttagg ttttgcattt gtgaaaaaat tcaggaatta aggaatgagg
      781 cagatcgagc aagaaaacaa aaaaattttg atttaatgaa agaaaaaact cttgaggcat
      841 ataaattatt tttctttttt gagcgagatt ttttgagcga aaatcgcgtt acttggaatg
      901 atatttcgta tagaaaaata gactccaaaa gtaaaaaaaa gactatgaat agtgtttcaa
      961 gtatcaaagc aatttttgaa gatttgaagc aaaagccgga gctttttgac aaatatagtt
     1021 tgatcttggc aatttcaagt ttgaccggct gccgtcctgc tgagatccaa aagggagtta
     1081 gcctggaagt aagcccccca ctattaaata tatgtattag cggggcgaaa gtgggcgaag
     1141 atcgagggca agaaaaaaga atattaaaat ttaatataaa ggaatttgca caaaatgagc
     1201 agttcaatct aattatgtca aaaataaaac aaaataataa gtttgaatat aaatgttcca
     1261 agcaggagta cgattcactg agacagtatt tatatattaa ccatcctgga ttttctctct
     1321 atacattaag acatagagca gccagtgaat taaaaaaaga cggcttttca gaagaaatga
     1381 tcgcagcttt tttaggtcat agagtaacca gatctctaga gaactatgga tacgcaagat
     1441 ctgggaaagg tggtccaaaa gtggcaggtg ttgagcattc aaacgccata aaaagcaata
     1501 caaggcgata taagagcaaa acaaccccag gagggggaaa cccttcccca actcgtaaac
     1561 gagccttaaa atgagtttta gagcgtttta caggagtcag caaaagctga ctctttttat
     1621 ttgatatgtg tgttagcctc gcgcgcgcgt cttgccggca cagatttgtg gtgactggct
     1681 tgtgtggtcg gtgtagttaa ctaatcaata tatgtgttag caggcggcac gcgcgcgagt
     1741 agtgcccgga acgggaaaag ggggagaggt tttgtcgagg tttgttggat caaatttttt
     1801 gaacaactat tgacagaaca agaagataag ttataacggt aacagataca aaaaaaccac
     1861 ccgaaggtgg tttttaggga caagaaaagt ttggcgactt ttcgagtctt gtaatcaagt
     1921 gagtcaagca ctggattacg atatgtaagt aagaagttaa cctacatttc gcaaaaagtc
     1981 aaacctctct ttgttacaac acccaaaaaa aagccatctt tttcaagtct ttaagcaacc
     2041 aaatttgcat attgtgtcgt gcctggaact gtggaatcaa gagggtccgg tttcggttgg
     2101 ttgttggcaa tagaagggga tctcacaaat tgccgaacct ggaggctgag ttctaaggtt
     2161 gttttggtga gcagtgttgt attggtaggc tatgagaaag cccagccata acagttataa
     2221 agctgtttaa aatggggtga actgtcgcaa aaaatgatta tgaactgtat ctagagggtg
     2281 tgagggggtg ccgtgtgttg gaaacgatac acagctcgac agtctagaac tttgtggtga
     2341 agtccaaaac tttgcaatca cacaaatcgc ccttagtgaa aattttctaa ttacctgatt
     2401 tttagggtag atccagaaaa tttttactag gggagacctt tgggtgaagc cctgtaaagg
     2461 gctgaacaca aaggtcttag cgaagccaca gcaaaacaat acagatctgt tatagagaaa
     2521 aagcaatgct gatctgtaga aaagagaaaa caatactaaa gaataaaaga aaaagagttc
     2581 ggagaaaaaa attcgaaatt tgattttttt ttgcaatttg ctatttaaat tccaaataac
     2641 atataaaaaa ggaagattta tggaaataga aaaattgatt gatagatatt tttttgcatt
     2701 agaaaagaaa attattgctg agatttataa taagcaaaca agagaagatt tggaaaagtt
     2761 tatatttaag tgcaatcaag agcaattaga gtttgaacga aggtttaata taatatgctc
     2821 tactgaatat tatgagaaac agaaacttat attttctata aattatattt cagcagaatt
     2881 tgagcaacga gaaaaattaa attattaaat ttgacaatat taaaataata tattaagcat
     2941 atatgtatta gcaaaataaa gagggcttaa tatatgacaa tatcaaaaaa aaatagtgaa
     3001 ttgagagagc gatttaaaga atacgccagt aataaaaaca tatttgatct tgctgatttg
     3061 agatatgaaa tattaaaaat atattatgat tttaaattaa aaaacgatat gaatgagcaa
     3121 gaaagaaaat ctcaagactc aagaagaaaa gcacatttaa cagcattaaa aaaaagaata
     3181 aaaagagaaa ttgtctcaaa aatagttatc gatttagtga aatattacaa tatagaaaaa
     3241 actacttttc attttttttc tcatatatgc actgagattt tagaaagaaa tgttgataac
     3301 agatacattc tgaataattt ttcaaatatg attttagacg aaaaaaaaga gttgacgaaa
     3361 ttaagtgaaa gcagaaatgc ttcaagcaaa atgattttag aaaactcata taatgaactt
     3421 gtttctatgt cacatattaa agacaaatta ttcagaaata acaatttcaa aactgcatat
     3481 ttaaaatgtt atgcttgtgc aaatgaggaa ttttcaagat ttaaagtttt tgctttccca
     3541 gacaattttg agacattaga ttttttattt gaagaagaaa gaataaaaaa agaaga
//
