LOCUS       NZ_CP083865            17041 bp    DNA     circular CON 06-APR-2024
DEFINITION  Enterobacter kobei strain 11743-yvys plasmid p11743_3, complete
            sequence.
ACCESSION   NZ_CP083865
VERSION     NZ_CP083865.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21379547
            Assembly: GCF_023333675.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 17041)
  AUTHORS   Liao,W., Cui,Y., Quan,J., Zhao,D., Han,X., Shi,Q., Wang,Q.,
            Jiang,Y., Du,X., Li,X. and Yu,Y.
  TITLE     High prevalence of colistin resistance and mcr-9/10 genes in
            Enterobacter spp. in a tertiary hospital over a decade
  JOURNAL   Int J Antimicrob Agents 59 (5), 106573 (2022)
   PUBMED   35307563
REFERENCE   2  (bases 1 to 17041)
  AUTHORS   Liao,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023333675.1-RS_2024_04_06
            Annotation Date                   :: 04/06/2024 07:15:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,381
            CDSs (total)                      :: 5,264
            Genes (coding)                    :: 5,129
            CDSs (with protein)               :: 5,129
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 39 of 135
            Pseudo Genes (incomplete)         :: 101 of 135
            Pseudo Genes (internal stop)      :: 17 of 135
            Pseudo Genes (multiple problems)  :: 18 of 135
            Pseudo Genes (short protein)      :: 1 of 135
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083865.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..17041
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="11743-yvys"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:208224"
                     /plasmid="p11743_3"
                     /geo_loc_name="China"
                     /lat_lon="30.15 N 120.10 E"
                     /collection_date="2011-09"
                     /collected_by="Yushan Cui"
     gene            13..237
                     /gene="pspB"
                     /locus_tag="LCD49_RS26765"
                     /old_locus_tag="LCD49_26830"
     CDS             13..237
                     /gene="pspB"
                     /locus_tag="LCD49_RS26765"
                     /old_locus_tag="LCD49_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460647.1"
                     /GO_process="GO:0009271 - phage shock [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="WP_004193245.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSSRGELAQSEQQRLVE
                     LNQDAQRMRERIQALEDILDAEHPNWRDR"
                     /name="pspB"
     gene            237..596
                     /gene="pspC"
                     /locus_tag="LCD49_RS26770"
                     /old_locus_tag="LCD49_26835"
     CDS             237..596
                     /gene="pspC"
                     /locus_tag="LCD49_RS26770"
                     /old_locus_tag="LCD49_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="WP_004193243.1"
                     /translation="MGGVNLNKKLWRIPQQGMVKGVCAGIAHYLDVPVKLVRILVVLSI
                     FFGLAFFTFVAYIVLTFVLDPMPDNVVSGEQQPSSGELLDAVDRELAASEKRLREMERY
                     VTSDTFTLRSRFRQL"
                     /name="pspC"
     gene            605..835
                     /gene="pspD"
                     /locus_tag="LCD49_RS26775"
                     /old_locus_tag="LCD49_26840"
     CDS             605..835
                     /gene="pspD"
                     /locus_tag="LCD49_RS26775"
                     /old_locus_tag="LCD49_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="WP_004193241.1"
                     /translation="MNNRWQRAGQRVKPGIKIAGKLVLLTALRYGPAGVAGWAVKSVAR
                     RPLKMLLALVLEPLLSRAATKLSKRYSGNQP"
                     /name="pspD"
     gene            complement(1024..>1212)
                     /locus_tag="LCD49_RS26780"
                     /old_locus_tag="LCD49_26845"
                     /pseudo=""
     CDS             complement(1024..>1212)
                     /locus_tag="LCD49_RS26780"
                     /old_locus_tag="LCD49_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /name="LacI family transcriptional regulator"
     gene            complement(1170..1991)
                     /locus_tag="LCD49_RS26785"
                     /old_locus_tag="LCD49_26850"
     CDS             complement(1170..1991)
                     /locus_tag="LCD49_RS26785"
                     /old_locus_tag="LCD49_26850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_013214003.1"
                     /translation="MDVLRFILRLPFILLRLAARSLVYLFTLLGFLLRPFTGRIRWAVP
                     GWVTFAGNQLARLERGGNRYPKTISALLLLTAAVAAGSYYTWHWYQNKPKPVDVAPLVV
                     QDISASVQRPSAVNYNRDDNSAQIVVVTFSRSAAPVTLIGKPVTAGITLTPAMEGEWQW
                     RNDRKLVFTAKKTFPMGKTYTVDMDAKTLLAPQVALTEKQKTFTTPEFYYRGGRAEFYQ
                     DPQDPMKKHAIIGLTFNAPADVKNLESRLSMTRDGKPVPYTVTVMNCCHLC"
                     /name="hypothetical protein"
     gene            complement(2186..3325)
                     /locus_tag="LCD49_RS26790"
                     /old_locus_tag="LCD49_26855"
     CDS             complement(2186..3325)
                     /locus_tag="LCD49_RS26790"
                     /old_locus_tag="LCD49_26855"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032097955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class C beta-lactamase DHA-1"
                     /protein_id="WP_004236386.1"
                     /translation="MKKSLSATLISALLAFSAPGFSAADNVAAVVDSTIKPLMAQQDIP
                     GMAVAVSVKGKPYYFNYGFADIQAKQPVTENTLFELGSVSKTFTGVLGAVSVAKKEMAL
                     NDPAAKYQPELALPQWKGITLLDLATYTAGGLPLQVPDAVKSRADLLNFYQQWQPSRKP
                     GDMRLYANSSIGLFGALTANAAGMPYEQLLTARILAPLGLSHTFITVPESAQSQYAYGY
                     KNKKPVRVSPGQLDAESYGVKSASKDMLRWAEMNMEPSRAGNADLEMAMYLAQTRYYKT
                     AAINQGLGWEMYDWPQQKDMIINGVTNEVALQPHPVTDNQVQPYNRASWVHKTGATTGF
                     GAYVAFIPEKQVAIVILANKNYPNTERVKAAQAILSALE"
                     /name="extended-spectrum class C beta-lactamase DHA-1"
     gene            3436..4311
                     /gene="ampR"
                     /locus_tag="LCD49_RS26795"
                     /old_locus_tag="LCD49_26860"
     CDS             3436..4311
                     /gene="ampR"
                     /locus_tag="LCD49_RS26795"
                     /old_locus_tag="LCD49_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator AmpR"
                     /protein_id="WP_004193231.1"
                     /translation="MVRRYLPLNPLRAFEAAARHLSFTRAAIELNVTHAAVSQQVRALE
                     EQLGCVLFTRVSRGLVLTHEGEGLLPVLNEAFDRIADTLECFSHGQFRERVKVGAVGTF
                     AAGWLLPRLAGFYDSHPHIDLHISTHNNHVDPAAEGHDYTIRFGNGAWHESDAELIFSA
                     PHAPLCSPAIAEQLQQPDDVHRFTLLRSFRRDEWSRWLDCAGGTPPSPSQPVMVFDTSL
                     AMAEAAQLGAGVAIAPVCMFSRLLQSGALVQPFAAEITLGGYWLTRLQSRTETPAMQQF
                     ARWLLNTAAA"
                     /name="ampR"
     gene            complement(4315..4680)
                     /gene="hypA"
                     /locus_tag="LCD49_RS26800"
                     /old_locus_tag="LCD49_26865"
     CDS             complement(4315..4680)
                     /gene="hypA"
                     /locus_tag="LCD49_RS26800"
                     /old_locus_tag="LCD49_26865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193196.1"
                     /GO_function="GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /GO_process="GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="WP_025999322.1"
                     /translation="MTDYDNGGRGKLSKALGAADIIREQAEQHGIARVTDVWLEVGALA
                     DVEESALHFCFDIACRDTVAQGCTLHIDVIPAQAWCWDCSREAEIMQHAGCCPHCGSER
                     LRISEGDDLRVKSLEGE"
                     /name="hypA"
     gene            4573..4908
                     /locus_tag="LCD49_RS26805"
                     /old_locus_tag="LCD49_26870"
     CDS             4573..4908
                     /locus_tag="LCD49_RS26805"
                     /old_locus_tag="LCD49_26870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679428.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA];
                     GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="WP_052238321.1"
                     /translation="MRYPRNAVLFSLFADNVRRTEGFTKLAPSAVVIIGYGIAFYFLSL
                     VLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSPSWKS
                     LRRPTPW"
                     /name="SMR family transporter"
     gene            4902..5741
                     /gene="sul1"
                     /locus_tag="LCD49_RS26810"
                     /old_locus_tag="LCD49_26875"
     ARG             4903..5738
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6146..7687
                     /locus_tag="LCD49_RS26815"
                     /old_locus_tag="LCD49_26880"
     CDS             6146..7687
                     /locus_tag="LCD49_RS26815"
                     /old_locus_tag="LCD49_26880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050481.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISCR1 family transposase"
                     /protein_id="WP_000050481.1"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASLE
                     AQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGARRM
                     AESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIKKAG
                     YTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYDELNT
                     LAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGPQQGRK
                     VFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPAVSEKRL
                     ALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVFAPNSKHR
                     VQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVCEHCGGHVK
                     VIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQSRLFD"
                     /name="IS91-like element ISCR1 family transposase"
     gene            complement(<7918..8376)
                     /locus_tag="LCD49_RS26820"
                     /old_locus_tag="LCD49_26885"
                     /pseudo=""
     CDS             complement(<7918..8376)
                     /locus_tag="LCD49_RS26820"
                     /old_locus_tag="LCD49_26885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /name="peptide ABC transporter permease"
     gene            complement(8363..9328)
                     /gene="sapB"
                     /locus_tag="LCD49_RS26825"
                     /old_locus_tag="LCD49_26890"
     CDS             complement(8363..9328)
                     /gene="sapB"
                     /locus_tag="LCD49_RS26825"
                     /old_locus_tag="LCD49_26890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="WP_004193276.1"
                     /translation="MIIFTLRRFLLLLVTLFFLTFIGFSLSYFTPHAPLQGASLWNAWV
                     FWFNGLIHWDFGVSSINGQLISEQLKEVFPATMELCILAFGFALMVGIPIGMLAGITRN
                     KWQDRCISALALMGFSIPVFWLALLLTLLFSLTLGWLPVSGRFDLLYEVKPVTGFAIID
                     AWISDSPWRDEMLVSAIRHMVLPVLTLSVAPTTEVIRLMRISTIEVFDQNYVKAAATRG
                     LSRLTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAIRQQDY
                     AAISAGVMVIGSLVIIVNVISDILGAMANPLKHKEWYALR"
                     /name="sapB"
     gene            complement(9325..10968)
                     /gene="sapA"
                     /locus_tag="LCD49_RS26830"
                     /old_locus_tag="LCD49_26895"
     CDS             complement(9325..10968)
                     /gene="sapA"
                     /locus_tag="LCD49_RS26830"
                     /old_locus_tag="LCD49_26895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460650.1"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="WP_004193272.1"
                     /translation="MRLVLSSLIVMAGFLSGQAAAAPEQASYADIRDSGFVYCVSGQVN
                     TFNPQKASSGLIVDTLAAQLYDRLLDVDPYTYRLVPELAESWEVLDNGATYRFHLRRDV
                     PFQKTAWFTPTRKLNADDVVFTFERIFDRQHPWHNVNGSSFPYFDSLQFADNVQSVRKL
                     DNNTVEFRLNQPDASFLWHLATHYASVMSAEYAAKLAKQDRQELLDRQPVGTGPYQLSE
                     NRAGQYIRLQRHEKFWRGTPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLSIL
                     RDDPRLRLTLRPGMNIAYLAFNTDKPPLNNPAVRHALALAINNQRLMQSIYYGTAETAA
                     SILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLQLWVPTSSQAWNPSPLKTAEL
                     IQADMAQVGVKVKIIPVEGRFQEARLMDMNHDLTLSGWATDSNDPDSFFRPMLSCAAIN
                     SQTNFAHWCNPEFDSILRKALSSQQLSSRIDAYDEAQNILARELPILPLASSLRLQAYR
                     YDIKGLVLSPFGNASFAGVSREKADEVKKP"
                     /name="sapA"
     gene            complement(11269..11661)
                     /locus_tag="LCD49_RS26835"
                     /old_locus_tag="LCD49_26900"
     CDS             complement(11269..11661)
                     /locus_tag="LCD49_RS26835"
                     /old_locus_tag="LCD49_26900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="WP_004193269.1"
                     /translation="MKISFAPLLFCFLLPSAFADDNGGLKKDTAPPPAHALDEGYRGTE
                     NARIMTVEQAKTMHDGATISLRGNLIEDQGGDKFIFRDKTGSINTLIPLAVFDGRTIKP
                     DQMISINGSLDAKMQPPVVRVNKIQK"
                     /name="YdeI family stress tolerance OB fold protein"
     gene            11793..12296
                     /locus_tag="LCD49_RS26840"
                     /old_locus_tag="LCD49_26905"
     CDS             11793..12296
                     /locus_tag="LCD49_RS26840"
                     /old_locus_tag="LCD49_26905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004193267.1"
                     /translation="MNREKTHFQGVKESLRFLNFESGFHKIGFICLLCIVFLASVGAFS
                     GGYLSEVTRKNTLKTTGLQYERFGRLQTEFKFKISAQKYDSVNKTLRIGGDFNKFYEME
                     NIWPQPDSMYSKGNDLYLVYNDSEAMQNFTIWLRVTPVKPGSVKSFLQLNGEPEIRFRQ
                     FIYP"
                     /name="hypothetical protein"
     gene            12306..12752
                     /locus_tag="LCD49_RS26845"
                     /old_locus_tag="LCD49_26910"
     CDS             12306..12752
                     /locus_tag="LCD49_RS26845"
                     /old_locus_tag="LCD49_26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="WP_004193264.1"
                     /translation="MEMVFRASAIYLILLVVFKIAGRRALLQMTSFDLILLLIISEATQ
                     QALLGNDFSITGAMITIVTLVTIDILFGFIKKKVGGAESVLDGSPVILLDHGIPLLDKM
                     KKVDVSLEDILVAARQNQGITESSKIKYAILERNGHISVIPEEN"
                     /name="YetF domain-containing protein"
     gene            12763..13278
                     /locus_tag="LCD49_RS26850"
                     /old_locus_tag="LCD49_26915"
     CDS             12763..13278
                     /locus_tag="LCD49_RS26850"
                     /old_locus_tag="LCD49_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="WP_017900989.1"
                     /translation="MNTEYNDAKTVNELTKRLANTLIDSDLTLTTAESCTGGKLAAALC
                     AQADTAEFYDIGVITFSDRAKQKMLDVRASTLKKYSAVSEQTVSEMSVGIRQQAETDIS
                     IAISGYAGPEGGEDGTPAGTVWFAWNFRGQIITKRECFSGDCQDVIEKAVRFSLAVLIE
                     EVSAWKNK"
                     /name="2-oxo-tetronate isomerase"
     gene            13455..14363
                     /locus_tag="LCD49_RS26855"
                     /old_locus_tag="LCD49_26920"
     CDS             13455..14363
                     /locus_tag="LCD49_RS26855"
                     /old_locus_tag="LCD49_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156638.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_004193260.1"
                     /translation="MTDLNKGKQTTNKTNSERFPQPPFPHQKQPFPGLAGKMQPRPDHG
                     EQSYQGSGRLNGRKVLITGGDSGIGRAVAIAYAREGADVAINYLPEEEDDAREVVELIK
                     KAGRKVAAIPGDIRDETFCNHLVKQAVDALGGLDILVNNAGRQQFCESIEDLTTEEFDA
                     TFKTNVYAMFWITKAAIPHFSRDSVIINTSSVQAYEPSEILLDYAQTKAAIVAFTKSLA
                     KQLAPKGIRVNAVAPGPYWTVLQCCGGQPQEKIEKFGANAPLGRPGQPVEIAPLYVTLA
                     AEENSYTSGQVWCSDGGTGTL"
                     /name="SDR family oxidoreductase"
     gene            complement(14459..15103)
                     /locus_tag="LCD49_RS26860"
                     /old_locus_tag="LCD49_26925"
     CDS             complement(14459..15103)
                     /locus_tag="LCD49_RS26860"
                     /old_locus_tag="LCD49_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB4"
                     /protein_id="WP_017900990.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRANLKDAIFKSCDLSMADFRNINALGIEIRHCRAQGSDFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFSS
                     FDWRAANVTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLLERLGIAVMG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB4"
     gene            complement(15186..16175)
                     /gene="pspF"
                     /locus_tag="LCD49_RS26865"
                     /old_locus_tag="LCD49_26930"
     CDS             complement(15186..16175)
                     /gene="pspF"
                     /locus_tag="LCD49_RS26865"
                     /old_locus_tag="LCD49_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="WP_017900991.1"
                     /translation="MVNFIMAGYKDNLLGEANSFIEVLEQVSRLAPLDKPVLVIGERGT
                     GKELIANRLHYLSTRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERA
                     DGGTLFLDELATAPMLVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPRMVSE
                     GTFRADLLDRLAFDVVQLPPLRERQSDIMLMAEHFAIQMCREIGLPLFPGFTDEAKETL
                     LHYRWPGNIRELKNVVERSVYRHGTSDYPLNEIVIDPFRRHAAPPLAQETRHTSVTLPL
                     DLREFQQQQEKEFLQTSLQQAKFNQKKAAELLGLTYHQLRALLKKHQI"
                     /name="pspF"
     gene            16331..16999
                     /gene="pspA"
                     /locus_tag="LCD49_RS26870"
                     /old_locus_tag="LCD49_26935"
     CDS             16331..16999
                     /gene="pspA"
                     /locus_tag="LCD49_RS26870"
                     /old_locus_tag="LCD49_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="WP_003020532.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVRS
                     NSARALAEKKQLSRRIEQASAQQAEWQEKAELALRKEKEDLARSALIEKQKLTDLIASL
                     EHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAASSSRDVRRQLDSGKLDE
                     AMARFESFERRIDQMEAEAESHNFGKQKSLDQQFADLKADDEISEQLAQLKAKMQQDKQ
                     "
                     /name="pspA"
     ARG             complement(14463..15103)
                     /name="qnrB4"
                     /gene="qnrB4"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB4"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(2190..3325)
                     /name="blaDHA-1"
                     /gene="blaDHA-1"
                     /gene_name="extended-spectrum class C beta-lactamase DHA-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 taaggagtac tcatgagcgc gctatttctg gccatcccgt taaccatctt tgtgctgttc
       61 gtcttaccga tttggctctg gctgcattac agcaatcgtt ccagtcgcgg cgagctggcg
      121 caaagtgaac aacagcgtct ggtagaactt aaccaggacg cacagcggat gcgtgagcgc
      181 attcaagcgc tggaagacat ccttgatgct gaacatccaa actggaggga ccgctaatgg
      241 ggggcgttaa tctgaataaa aaactgtggc gtataccgca gcagggcatg gtgaaagggg
      301 tttgcgcggg tattgcgcat tatctcgatg ttcctgtgaa actggtgcgg atcctggtgg
      361 tgctgtccat cttttttggt ctggcctttt tcacctttgt cgcctacatc gtactgacgt
      421 ttgtgttgga tccgatgcca gacaacgtgg tttccggaga acagcagcca tcaagcggtg
      481 agctattgga tgctgtcgat cgtgaactgg ctgcaagtga aaagcgtttg cgtgagatgg
      541 agcgttatgt aacgtctgat acttttacat tacgcagccg cttccgccaa ttgtgaggtt
      601 agaaatgaat aatcgctggc aacgtgccgg gcaaagggtt aagccgggca ttaaaatagc
      661 aggtaagctg gtgctactga ctgcgctgcg ctatggcccg gctggggtag cagggtgggc
      721 ggtgaagtcc gtggcccgac gtccgctaaa aatgttgctg gcgttagtat tggaaccttt
      781 gttgagccgg gcagcgacaa aattgtcgaa acgctattca ggtaatcaac cctgatatcc
      841 gaaacaacaa aagcaagcaa catttagtca acattccccc agttaatttg cggcagagta
      901 gtctcttgtg tcatgggatc gctaccaata gaaatgaagg tgaaagcgac agactccatt
      961 atgcaggtat cggtgtgaat tacgcattct aaaatgcact ggggcattgt tatgccccag
     1021 tttttatacc agtgagtcgg ctaacgatat ttgtgtaccc acggtgatat tttgtaaacc
     1081 tttctcaccg gcaatcaggc taaataataa attacagctt tgttcgccga gctgctgggt
     1141 tgggacatca atgcctcccg gcacgggcgt cagcataaat gacaacagtt cattacagtg
     1201 acggtatacg gcaccggctt gccgtcacgg gtcatagaca gacgcgattc cagatttttg
     1261 acatcagcgg gggcattgaa ggtcaggccg ataatggcgt gttttttcat cggatcttgc
     1321 ggatcctgat agaactccgc ccggccgccg cgataataaa actcaggggt ggtaaaggtt
     1381 ttttgtttct ctgttaacgc cacctgcgga gccagcagtg ttttggcatc catatcgacg
     1441 gtgtaggttt tccccatcgg gaaggttttt ttcgccgtga ataccagctt acggtcatta
     1501 cgccactgcc actcaccttc cattgcgggg gtcagggtaa taccggcggt gaccggttta
     1561 ccgatcagcg ttaccggtgc ggcagagcgg gagaatgtca ccaccacaat ctgcgctgaa
     1621 ttatcatcac ggttataatt aaccgccgaa gggcgctgca cagacgcgga aatatcctgc
     1681 accaccagcg gcgcaacatc caccggcttc ggtttgttct gataccagtg ccaggtgtaa
     1741 taactgcccg ctgccaccgc agcagtcagt aataataagg cagatatggt tttcggatag
     1801 cggttgcccc cccgctccag ccgggcaagc tgattaccgg caaaggtcac ccagcccggc
     1861 accgcccagc ggatccgtcc ggtgaacggg cgcagcagaa aacccagcag agtaaagaga
     1921 taaacaagac tgcgagccgc cagacgcagc aaaataaaag gtaagcggag gataaaacgc
     1981 agaacgtcca tgacaacatt ttcctctgtg tgttgagact gacatcagtt tgccgcagca
     2041 cacagtgaaa tccggttcag ccggaagctg tcagtgcccg atactctcat cctccataaa
     2101 acagcccata aagcaaatta ttgcataagg aataatctta aattacggcc ccggcgtatc
     2161 cgcaggggcc tgttcaggaa aaaaattatt ccagtgcact caaaatagcc tgtgcagctt
     2221 tgactctttc ggtattcggg tagtttttat tcgccagaat cacaatcgcc acctgttttt
     2281 ccggaataaa ggcgacatag gcgccgaaac cagttgttgc gcccgtttta tgcacccagg
     2341 aagcacggtt atacggctga acctggttgt ctgttaccgg atgcggctgc aatgcgacct
     2401 cgttggtcac accgttaatg atcatatctt tctgctgcgg ccagtcatac atttcccagc
     2461 ccagcccctg gttaatcgcg gcggttttat agtagcgggt ctgggcgaga tacattgcca
     2521 tttccagatc cgcattaccg gcccgtgacg gctccatatt catttccgcc cagcgcagca
     2581 tatcttttga ggcggatttc acgccgtaag attccgcatc aagctgtccc ggcgacacgc
     2641 ggaccggttt tttgttttta taaccgtacg catactggct ttgcgcactt tccggcacag
     2701 taataaaggt gtgagataac cccagcggtg ccaggatccg tgcagtcagc aactgctcat
     2761 acggcatccc cgccgcgttt gcggtcagag caccaaacag gccgatactg ctgtttgcat
     2821 acagacgcat atcgcccggt ttccgggacg gctgccactg ctgatagaaa ttcagcagat
     2881 ccgcacggct ttttaccgca tccggcacct gtaacggcag tccgcctgcg gtataggtag
     2941 ccagatccag caatgtgatc cccttccact gcggcagagc cagctccggc tggtattttg
     3001 ccgccggatc attcagcgcc atctcttttt tcgccacaga aaccgcaccc agcacacctg
     3061 tgaaagtttt acttacagat ccgagctcaa atagtgtatt ttcagtgacc ggctgttttg
     3121 cctgaatatc ggcaaaacca taattgaaat aatagggctt accctttacg gagacggcaa
     3181 ccgccatccc gggaatatcc tgctgtgcca tcagcggttt aatggtgctg tccaccaccg
     3241 ccgcgacatt atcagcggca gaaaaccccg gggcggaaaa cgccagcaga gcggaaatca
     3301 gtgttgcaga taacgatttt ttcatcagaa ttaaccttcc gtgtgtgagt aacggcggca
     3361 agtatcgtca gattcaccgg acagaacaaa cggttattta taacaagagc ctaaagaaaa
     3421 acttacaggt ggattatggt cagacgttat ctccccctta acccgctgcg cgcctttgag
     3481 gccgccgccc gtcatctcag ttttacccgc gcggcgattg agctgaatgt cacccatgcc
     3541 gccgtcagcc agcaggtcag ggcgctggaa gaacaactcg gctgtgtgct gtttacccgc
     3601 gtctcgcgcg ggctggtgct gacccatgaa ggtgagggat tactgccggt gctcaatgag
     3661 gcgtttgacc ggattgcgga tactctggag tgtttttctc acgggcagtt ccgtgagcgg
     3721 gtgaaagtcg gtgcggtggg aacatttgcc gcaggctggc tgctgccgcg tctggccgga
     3781 ttctatgaca gccatccgca tattgatctg catatctcca cccataacaa tcatgtggac
     3841 ccggcggcgg aagggcatga ttatacgatc cgtttcggta acggcgcgtg gcatgagtca
     3901 gatgcggaac tgattttcag tgcaccacac gctccgctgt gctcaccggc cattgcagaa
     3961 cagttacagc agccggatga tgttcaccgc tttaccctgc tgcgctcatt ccgccgggat
     4021 gaatggagcc gctggctgga ttgtgcgggc ggcacaccgc cttccccgtc acagccggta
     4081 atggtgttcg atacctcact ggccatggcc gaggcggcac aactgggtgc cggggtagcg
     4141 atcgcaccgg tatgtatgtt cagccgcctg ttacagtcag gcgcactggt acagccgttt
     4201 gccgcagaaa tcaccctcgg cggctactgg ctgacgcggt tacagtcccg tacggaaacc
     4261 ccggccatgc agcaattcgc ccgctggctg ctgaatacgg cggcggcgta aaactcactc
     4321 accttccagg ctttttaccc gcaaatcatc accttcactg atgcgcagcc gttcactgcc
     4381 gcagtgcgga cagcatccgg cgtgctgcat gatttcggcc tcacggctgc aatcccagca
     4441 ccatgcctgt gccgggataa catcaatatg cagtgtgcag ccctgcgcca cggtatcacg
     4501 gcaggcgata tcaaaacaga aatgcagtgc actctcctca acatccgcca gtgcgccgac
     4561 ttccagccag acatccgtta cccgcgcaat gccgtgctgt tcagcctgtt cgcggataat
     4621 gtccgccgca ccgagggctt tactaagctt gccccttccg ccgttgtcat aatcggttat
     4681 ggcatcgcat tttattttct ttctctggtt ctgaaatcca tccctgtcgg tgttgcttat
     4741 gcagtctggt cgggactcgg cgtcgtcata attacagcca ttgcctggtt gcttcatggg
     4801 caaaagcttg atgcgtgggg ctttgtaggt atggggctca taattgctgc ctttttgctc
     4861 gcccgatccc catcgtggaa gtcgctgcgg aggccgacgc catggtgacg gtgttcggca
     4921 ttctgaatct caccgaggac tccttcttcg atgagagccg gcggctagac cccgccggcg
     4981 ctgtcaccgc ggcgatcgaa atgctgcgag tcggatcaga cgtcgtggat gtcggaccgg
     5041 ccgccagcca tccggacgcg aggcctgtat cgccggccga tgagatcaga cgtattgcgc
     5101 cgctcttaga cgccctgtcc gatcagatgc accgtgtttc aatcgacagc ttccaaccgg
     5161 aaacccagcg ctatgcgctc aagcgcggcg tgggctacct gaacgatatc caaggatttc
     5221 ctgaccctgc gctctatccc gatattgctg aggcggactg caggctggtg gttatgcact
     5281 cagcgcagcg ggatggcatc gccacccgca ccggtcacct tcgacccgaa gacgcgctcg
     5341 acgagattgt gcggttcttc gaggcgcggg tttccgcctt gcgacggagc ggggtcgctg
     5401 ccgaccggct catcctcgat ccggggatgg gatttttctt gagccccgca ccggaaacat
     5461 cgctgcacgt gctgtcgaac cttcaaaagc tgaagtcggc gttggggctt ccgctattgg
     5521 tctcggtgtc gcggaaatcc ttcttgggcg ccaccgttgg ccttcctgta aaggatctgg
     5581 gtccagcgag ccttgcggcg gaacttcacg cgatcggcaa tggcgctgac tacgtccgca
     5641 cccacgcgcc tggagatctg cgaagcgcaa tcaccttctc ggaaaccctc gcgaaatttc
     5701 gcagtcgcga cgccagagac cgagggttag atcatgccta gcattcacct tccggccgcc
     5761 cgctaaatat ctccttttgg gttgttaata aaacatccaa taagttgact gtgcgtgaaa
     5821 aagaaagttt tgtgtgatgg cgttgaagat cgcaccgtta agctcttatg tgggatggtg
     5881 cagagctcga cgactaccga taaaacgcaa ccgccgcaaa cagacaagaa aaagccccaa
     5941 ctgataacag ttggggcttc agtattgtga ttggtggagc aatagcaccc tgaacccaaa
     6001 accttctcgc tcaaccggta gtggctgata acaactcgtg agggctattg cgggttaagc
     6061 atttagcgat gtctagggcc agactggacg tctgaacgca agccgctgat actgtacata
     6121 accacagtat cagcggagga tacccatgtc gctggcaagg aacgccacgg cgagtcaatc
     6181 gcccactcaa acaaacggtt acgaacgcca ccaacccgac cagacgctgc tctaccagct
     6241 ggttgagcag cactacccag ccttcaaagc ctcactcgaa gcccaaggtc aacacctgcc
     6301 tcgctacatc caacaagaat tcaacgacct cctccaatgt ggccgtctgg agtatggttt
     6361 catgcgggtt cgctgcgagg attgtcatca cgagcgtctg gtcgccttca gctgtaaacg
     6421 acgcggcttt tgccctagct gcggtgcccg ccggatggcc gagagtgcgg cgctgctgat
     6481 agacgaagtc ttccccaagg agcccattcg ccagtgggtg ctcagctttc ctttccagct
     6541 acgctttttg ctggctcgcc atccccagct gatgggccag gtcttgagta tcgtctatcg
     6601 tacactctca actcatctga tcaaaaaagc cggttacacc aaagcctctg cacaaactgg
     6661 ctcagtgact cttatccaac gctttggctc cgcgctaaat ctcaatgtcc actaccacat
     6721 gctgtttctc gatggtgtct atgccgaaga tgactatggc aagcaacgct tccatcgtgt
     6781 caaggcaccc acttacgatg agctgaatac gctcgctcac accctcagcc atcgcatcgc
     6841 tcgctgcatg gaaaagcgtg ggattttgga gcgtgatgcc gagaatacgt ggttgacact
     6901 ggaagagggc gaagacgata cgctgactca attacatggt gcttcggtta cgtatcgcat
     6961 tgccgtcggc ccccagcaag ggcgcaaagt cttcaccctg caaaccttgc cagggcgtga
     7021 ggataaagcc gactcaagca gtcgagtagc caaccatgct ggtttctcgc tacacgccgg
     7081 tgtgatggcc gaagcgcatc agcgggataa gcttgagcgc ttgtgtcgct acattagtcg
     7141 gccagcggtt tcagaaaaac gtctggcatt aaccgccaat gggcaggtgc gttacgagct
     7201 caaaactccg taccgcaatg gcaccaccca tgtgatcttc gagccgctgg acttcatcgc
     7261 caaactcgct gcgttggtac ctaagccgcg agtcaacctc acacgcttcc acggcgtctt
     7321 tgcaccgaac agcaaacacc gagttcaagt aacacccgcc aagcggggca agaagcccga
     7381 caaatcggaa ggtctcgata ctaactggcg tgacaagagt cctgcagagc gccaccgcgc
     7441 catgacctgg atgcaacgcc tcaagcgagt cttcaatatt gatattgaag tctgcgaaca
     7501 ctgcggcggt cacgtcaaag tgattgccag catcgaagat ccgaaggtca ttgagcagat
     7561 tctcaagcat ctgaaacaga aaacagccaa ggcgaatgcc gccaagcagc gtgagctgcc
     7621 accagaacga gcgccgccac tgactcccag cctgttcgat ccatcacaga gtcgtctctt
     7681 tgactgacga ccccaaatcc aacactgctc aacactgcca acttttaaac ggggcggtgg
     7741 ggcagtttgt atctctcgag ctatcaggct agagatttta ccgccaaatc gaaccttatt
     7801 agagcggttt aggctggacc ggcagttaaa attggggctt gagcggtaaa cgagtgaggg
     7861 aatttcaggt aagatacttc ggatgaggag caaaaaggtg gtttatactt cctataccca
     7921 agccaccaca ataatcgcca gcaacagtga agggatcgac agcagggtgt ccagaatgtg
     7981 attcagcacc gcggagcgca aaccgtgtgt tgccccggca accacgccaa gcaataagcc
     8041 acacaacgtg gcggcaaggg tgacgacaaa cgcaccgccc acggtcgggg ccgcaccgct
     8101 aagcaggcgg cttaaaacat cacgccccag gtcatcagtc ccgaggaaga aagagacttc
     8161 gccatagcgt gaccatgacg gcggcaaaag ctggtagcca agaaactgct ggtcgatgcc
     8221 ataaggcgcg aaccaaccac caaaaataca cagaattgcc agtccagcac agccatacag
     8281 gccgatcatt gcggttgcat cgccgtaaaa attgcgccat gcggtgcgca gcgtaccagg
     8341 cgggcgcttt tcgctgtaga cgctatcgta aggcatacca ttccttatgt ttcagagggt
     8401 tagccatagc acccaaaata tcagagatca cgttaacaat gataaccagt gagccaatca
     8461 ccatcacacc cgccgaaatc gccgcgtaat cctgctggcg aatcgcgtta atcagccaac
     8521 ggccaagacc tggccagcta aagaccatct cggtgatcat cgccagcgtc agcatggtgg
     8581 aaaactgtaa ccccaggcgc gggataaccg gtgggagagc gttgtgcagt acgtgacggc
     8641 gtaaaatcgt taaccgtgac agaccacgag tcgccgccgc tttgacatag ttttgatcaa
     8701 agacctcaat agtgctgata cgcatcagac gaataacttc cgtcgtcggt gccacggaga
     8761 gcgttaacac cggaagcacc atatggcgga tggcactgac cagcatttca tcgcgccagg
     8821 gcgagtcgga gatccaggcg tcaataatgg caaacccggt tacgggtttt acttcataaa
     8881 gcagatcaaa acgccctgat acgggtaacc agccgagggt tagtgagaat agcaacgtca
     8941 gcagcagcgc cagccaaaaa accggaatag agaagcccat cagagccaat gcgctgatgc
     9001 aacgatcctg ccatttatta cgcgttatgc ccgccaacat gccgatgggg atcccgacca
     9061 ttagtgcaaa accaaatgcc agaatgcaca gttccattgt tgccgggaac acctctttca
     9121 gttgttctga gatcaactgg ccgttaatac tggacacacc aaaatcccag tgaatcaggc
     9181 cattgaacca gaatacccaa gcgttccaca gcgatgcgcc ctgtaacggc gcatgggggg
     9241 tgaaataact cagactgaag ccaataaagg tcaggaagaa caaagtgacc agcaataata
     9301 aaaatcggcg tagggtgaag ataatcatgg ttttttcacc tcgtcagcct tttccctgga
     9361 aaccccggca aaagaagcgt tgccgaacgg actgagcacc agccctttga tgtcatagcg
     9421 gtaagcctgc aaacggagcg aagaggccag aggcaggatc ggcaactcgc gcgcaagaat
     9481 attttgcgct tcgtcgtagg catcaattcg cgaggagagt tgttgcgaag agagggcttt
     9541 gcgcaaaatg ctgtcaaact cgggattgca ccagtgggca aaattggtct gcgagttaat
     9601 ggcggcacag ctaagcatcg gtcggaaaaa gctgtcagga tcgttactgt cagttgccca
     9661 accggacaac gtcagatcat ggttcatatc cattaatcgc gcctcctgaa aacggccctc
     9721 taccgggatg atcttcactt tcacacccac ctgagccata tcagcctgaa tcagctctgc
     9781 ggttttcagc ggactggggt tccaggcctg agagctggta ggtacccaga gttgtaacgt
     9841 caggttttcc agccccagcg cttttaactg ctcgcgcgat tttgccgggt tgtactcggt
     9901 aattttagct tcgttgtcat aagcccacga ggcgcgaggc aaaatagatg ccgcggtttc
     9961 cgccgtaccg tagtaaatag attgcattaa ccgttggttg ttaatggcca gcgctagcgc
    10021 atgtcgtacg gcagggttgt tcaacggcgg tttatccgtg ttgaacgcca gataggcgat
    10081 attcatcccc ggacgcagcg tcaggcgcag ccgcgggtca tcacgcagta tgctgagctg
    10141 gctggcggca ggccatgcca ggacgtcgca ctcgccggtc agtaatttcg ataagcgtcc
    10201 ggtcccgcca gagccgagat ccaccacgac ctgcggcatc aagggggtac cacgccagaa
    10261 tttttcatgc cgttgtaggc gaatatattg gcctgcacga ttttctgaaa gttggtacgg
    10321 gcctgtgccg acgggttgtc tgtcgagtaa ttcctggcga tcctgttttg ctaatttggc
    10381 ggcatattcg gccgacatca cagaggcata atgtgtggca aggtgccaca agaaggaggc
    10441 gtcgggttga tttaagcgaa attcaacggt gttgttgtcc agtttacgca cgctttggac
    10501 gttatcggcg aactgcaggc tatcaaaata aggaaaactg ctaccattga cattatgcca
    10561 cggatgctgg cgatcaaaga tgcgctcgaa ggtaaatacc acatcgtccg catttagttt
    10621 gcgggtgggc gtgaaccagg cagtcttttg aaacggaaca tcgcgtcgta aatggaagcg
    10681 gtaggttgcg ccgttatcta atacttccca gctttcagcc agttctggca ccaggcgata
    10741 ggtgtaggga tcgacatcaa gcagtcggtc atacaattgg gccgctaatg tgtctacgat
    10801 gagaccgctg cttgcttttt gtggattgaa cgtattgacc tgcccgctaa cgcaatagac
    10861 aaagccactg tcacgaatat cagcgtacga ggcctgctca ggcgcagctg cagcctgacc
    10921 actcagaaat ccagccatca cgatcagaga tgataaaacc aggcgcataa ttttaaaggg
    10981 ttatatataa agaagctatc ttactaatac ttaatgacat ttgccattac cgtttgtttt
    11041 tggggcagtg gggttgataa ccgcgaattc gacatcgcgt actggtcaaa attcataccc
    11101 gctatccact ttgcatatac tcttgtagct atcttagcat tttcatggcc cctctgactc
    11161 gcttaaacgc tggatttgct gccagagaga ttatgtgagg tgtctgacgg ggaaaatgaa
    11221 tctgttgtgt gggaggcggg aagagtgaat ttcttcccgg ctgaggtatt acttctggat
    11281 tttattaacg cgcaccaccg gcggttgcat tttagcgtca aggctaccgt taatgctgat
    11341 catttgatcg ggttttatcg ttcggccatc aaagaccgca agcgggatta gcgtattgat
    11401 actgccggtt ttgtcacgga agataaactt atctcctccc tgatcctcaa tgagattacc
    11461 tcgtaaagag atggtcgcac cgtcatgcat ggttttagct tgttcgacgg tcatgattct
    11521 tgcattttca gtgccgcgat agccttcatc cagtgcatgg gcaggtggcg gggcggtatc
    11581 ttttttcaat ccgccattat catcggcaaa cgctgatggc aataaaaaac agaacagtaa
    11641 gggggcgaac gatattttca tagagcctcc attgaaattg atttgactta ttaagtttgg
    11701 tagctattta ttaatctggc gagtgggata agtctcaaat tgtaattttt caataaaaca
    11761 acggcttggt gttatataag cgaggcgggg agatgaacag agagaaaacc cattttcaag
    11821 gagtaaaaga gagcctgcgt tttctgaatt ttgaatctgg ctttcacaag attggtttta
    11881 tctgtttgct ctgcattgtt tttcttgctt cggtgggagc gttttccggc ggatatttaa
    11941 gtgaggtgac gagaaaaaac accttaaaga caacagggtt acagtacgaa aggtttgggc
    12001 gcttgcaaac ggaatttaaa tttaaaatct ctgcacaaaa gtatgattct gtgaacaaga
    12061 cgttgcgcat tggcggcgat ttcaacaaat tttatgaaat ggaaaatatt tggccgcagc
    12121 cagacagcat gtacagcaag gggaacgatc tttacctcgt ctacaatgat tctgaagcaa
    12181 tgcagaactt tactatctgg ctgcgggtta cgcccgtgaa gccaggaagc gtaaagagtt
    12241 ttctccagct aaatggcgaa cctgaaatcc gcttcagaca atttatttat ccctaggagg
    12301 tgagtatgga gatggtcttc cgggcatccg ccatttacct tattttgctg gtggttttca
    12361 aaatagcagg tcgccgggca ttattacaaa tgaccagctt cgatctgatt ttattactga
    12421 ttatcagtga agcaactcaa caagccttgt tgggtaatga tttttcgata accggcgcca
    12481 tgatcacgat tgtcactctg gttactattg atattctgtt cggtttcatt aaaaaaaagg
    12541 tgggtggggc cgaatccgtg ctggatggtt ccccggttat cctgcttgat cacgggatcc
    12601 ctttattaga taaaatgaag aaagtggatg tctctcttga agatatatta gtcgctgcgc
    12661 gacaaaatca ggggattacc gaatcaagta aaatcaagta tgccattctg gaacgcaacg
    12721 gacatatttc tgtcattcct gaagagaatt agcgaggcgg taatgaatac ggagtacaat
    12781 gacgcaaaaa cagtcaatga actgacaaaa cggcttgcaa acacgctcat tgatagcgat
    12841 ttaacgttga ccaccgcaga atcctgtaca ggaggaaagc tggccgcggc gctttgcgca
    12901 caggcggata ccgcagaatt ttatgatatc ggcgttataa cctttagcga tcgcgccaag
    12961 cagaaaatgc tcgacgtgcg ggccagtacg ctgaagaaat atagcgccgt tagtgagcaa
    13021 acggtcagtg agatgtctgt aggaatacgt cagcaagccg aaaccgatat cagtatagcc
    13081 atcagcggat atgcagggcc ggagggtggg gaggatggta ctcccgcagg aaccgtgtgg
    13141 tttgcgtgga atttccgcgg acagataata acaaaacgtg agtgtttttc cggggattgc
    13201 caggacgtca ttgaaaaggc ggtacgtttt agcctggcgg tattgattga agaagtgtca
    13261 gcctggaaga acaaataaat aaaccaggac ttatcctagg aaatatttca atgctatgtg
    13321 aagttatcgt cgtgagagcc aggttaatat taggagaaaa ttaagtggta ggtaaattat
    13381 aaagaccatg tcattcattt tgttctacgc ttattagtct ttgcgatgaa tgatgactca
    13441 cgagaggtga ttgaatgact gacctaaata aaggaaaaca gaccaccaat aaaactaatt
    13501 cagagcgttt tccgcagccg ccttttccac atcaaaaaca accttttccg gggctcgccg
    13561 gaaaaatgca accgcgcccc gatcatggcg aacaaagcta tcagggaagc ggcaggttaa
    13621 atggtcgcaa ggtattaatt accggtggtg attcaggtat tgggcgtgct gttgccattg
    13681 cctatgcccg cgaaggcgcc gatgtcgcga ttaattattt acctgaagaa gaggacgatg
    13741 ctcgtgaggt tgttgagttg ataaaaaaag ctgggagaaa agttgcggcg attcccggcg
    13801 atattcgtga cgaaactttt tgtaaccatc tggtaaaaca ggcggtagac gcgctgggag
    13861 ggttggatat cctggtgaat aacgcaggcc gtcagcaatt ttgtgaatcg atcgaagacc
    13921 tcaccacaga agaatttgat gcgacattca agaccaatgt ctacgccatg ttttggatca
    13981 ccaaggcggc tattccccat ttctcccgag acagcgtgat tattaacacc tcatcggtac
    14041 aggcttatga gccgagtgag atcctgcttg attatgctca gaccaaggcg gcgatagtgg
    14101 catttactaa atcgctggcg aagcaactgg cccctaaagg gatccgtgta aatgctgtcg
    14161 cgcctgggcc gtactggacg gtattgcagt gctgcggcgg ccaaccacag gaaaaaattg
    14221 agaaatttgg tgcaaatgcg ccgcttggtc gccctggcca accggtagaa attgctccgc
    14281 tgtatgtcac tttagcggct gaagagaaca gctatacatc cggtcaggta tggtgttctg
    14341 acggggggac cggaaccctc tgacgtttat gcccccgaag tcgaaacgtc tgagggggaa
    14401 agccagcccg gacaatattg tataagggca gggggcggca tataccgctc cctgaacatt
    14461 aacccatgac agcgatacca agacgttcca ggagcaacga tgcctggtag ctgtccagtt
    14521 tgacgccttg caaatcaacc ccgcggatat ctaaatcgcc cagttccgaa ttggtcaaat
    14581 cacagtgcgt aacgtttgct gctcgccagt cgaaggatga aaactcgccg ccagagaggt
    14641 ctgatccact gaacgttgcg cccagcacct gagtacccat ccagcggttt tcccacagct
    14701 cgcacttttc cagtacgact tttgaaaagt tggcgtagct taagttggta ttggtgatat
    14761 aggcgctaca aaaccaggtg cgggtggtga tcatattcat aaaacttgcg ccgcgaaaat
    14821 ctgacccttg tgcccggcag tggcgaattt cgattcccag cgcattgata ttcctgaaat
    14881 cagccatgga gagatcacaa cttttgaaaa tggcatcttt caggttagcg cgactaaaat
    14941 tacatccttt ctgactttct cgatcataaa actggcagcc aataaattca gtgccgctaa
    15001 ggtcggcacc cgaaaaatca cagttgaaaa atgtgctatt ttcaactttt tcaccggtga
    15061 acctgtttct gtcaattttt tcgccaacta acgccagagt catcatgatt aacctgtttt
    15121 tttatacagt gattgcaaca tggtaaactg ttttaactct cgcgtccaac gtctgcccct
    15181 gcacgctaaa tttggtgttt cttcagtaat gcgcggagtt gatggtaggt cagacctaat
    15241 aattcagcgg cttttttctg attaaatttc gcctgctgta agctggtttg caaaaattcc
    15301 ttctcctgct gctgttggaa ttcacgcaga tccagcggta gggttactga tgtatgtctg
    15361 gtttcttgcg ccagcggcgg tgcggcgtga cgcctgaacg ggtcaatcac aatctcattc
    15421 agtgggtaat cgctggtccc gtgtcggtat accgaacgct ccacgacgtt tttcagttca
    15481 cgaatattgc ccggccaacg gtagtggagg agcgtttctt tggcctcatc ggtgaaccca
    15541 ggaaacaacg gtaacccgat ctcacggcac atctgaatgg caaaatgttc agccattaac
    15601 atgatatcgc tttgacgttc gcgtaacggg ggaagttgca ccacgtcaaa ggccagacga
    15661 tccagcaggt cggcgcggaa cgtgccttca ctcaccatgc gtggcaggtc ggcgttggtt
    15721 gcgcaaacca gtcttacatt gacctgcaga ggttggctac cgcctacgcg ttccagctct
    15781 ccgtactcaa taacgcgcag cagtttttcc tgtaccagca tcggggcggt tgccagttca
    15841 tcaagaaaca gtgtgccgcc atcggcgcgc tcgaaccgac ctgggtgacg tttctgcgcg
    15901 ccggtaaagg cgcccgcttc gtgcccaaag agttcggaat caagtaaatt ttcgttgagc
    15961 gcggcgcagt taagtgaaat aaacgggcct tgccaacggg ttgagaggta gtgcaggcgg
    16021 ttggcgataa gctctttccc tgttcctcgt tccccaatga ccagcacagg tttatccagc
    16081 ggcgcgagac gagaaacctg ctccagcact tcaataaagc tgttcgcttc gcccagcaga
    16141 ttatctttgt atcctgccat gatgaaattc accatttgtt agtgttattc accaatttac
    16201 cctatttatc ccgacaggta aaattagtta ttttcaaata tttcaatgaa ttaaaaagtt
    16261 ggcatgcgaa gtgcattaga acagcagcag ggcattgccc gatatcagaa cagtaagtga
    16321 ggattacatt atgggtattt tttctcgttt tgccgacatc gtgaacgcca acattaatgc
    16381 gttgctggaa aaggctgaag atccgcagaa gctggtgcgg ttgatgatcc aggaaatgga
    16441 agacacgctg gttgaggtgc gctccaactc cgcacgcgcg ctggcagaaa agaaacagtt
    16501 atcgcgtcgt attgaacagg ccagcgccca acaggcagag tggcaggaaa aagcagagct
    16561 ggcgctgcgt aaagaaaaag aagatttggc gcgttctgcg ttaatcgaaa agcagaaact
    16621 gactgacctg attgcctccc tggagcatga ggtgacgctg gttgacgata cgctggcgcg
    16681 tatgaaaaaa gagatcggtg aactggaaaa caaactcagt gaaacccgtg ctcgtcagca
    16741 ggcattgatg ttgcgccatc aggcggcgag ttcttcccgt gatgttcgtc gtcaactgga
    16801 cagcggcaaa ctggatgaag cgatggctcg ctttgaatct tttgagcgtc gtatcgatca
    16861 aatggaagct gaagctgaaa gccataactt cggtaagcag aagtctttgg atcagcaatt
    16921 tgctgacctg aaagcagatg atgaaatcag cgagcagttg gcgcaattga aagccaaaat
    16981 gcagcaagac aagcaataat aatatctggc ggtgcccgaa cgcgccgccg ctcatcacct
    17041 g
//
