LOCUS       NZ_CP083861             4801 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Enterobacter kobei strain 12379-yvys plasmid p12379_4, complete
            sequence.
ACCESSION   NZ_CP083861
VERSION     NZ_CP083861.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21379548
            Assembly: GCF_023023705.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Liao,W., Cui,Y., Quan,J., Zhao,D., Han,X., Shi,Q., Wang,Q.,
            Jiang,Y., Du,X., Li,X. and Yu,Y.
  TITLE     High prevalence of colistin resistance and mcr-9/10 genes in
            Enterobacter spp. in a tertiary hospital over a decade
  JOURNAL   Int J Antimicrob Agents 59 (5), 106573 (2022)
   PUBMED   35307563
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Liao,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023023705.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 07:11:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,813
            CDSs (total)                      :: 4,697
            Genes (coding)                    :: 4,629
            CDSs (with protein)               :: 4,629
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 15 of 68
            Pseudo Genes (short protein)      :: 1 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083861.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="12379-yvys"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:208224"
                     /plasmid="p12379_4"
                     /geo_loc_name="China"
                     /lat_lon="30.15 N 120.10 E"
                     /collection_date="2012-05"
                     /collected_by="Yushan Cui"
     gene            join(4767..4801,1..235)
                     /locus_tag="LCD50_RS24020"
                     /old_locus_tag="LCD50_24065"
     CDS             join(4767..4801,1..235)
                     /locus_tag="LCD50_RS24020"
                     /old_locus_tag="LCD50_24065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="WP_113519623.1"
                     /translation="MKVKNRKRELTAKEYAEQYEISVRTVKRYFSQDREDYEKEAKQRR
                     LKAFVLRESGMKWADVGIALGTTKHGAIALYKRYQQIDAPKEEA"
                     /name="plasmid replication protein"
     gene            complement(242..1720)
                     /locus_tag="LCD50_RS24025"
                     /old_locus_tag="LCD50_24070"
     CDS             complement(242..1720)
                     /locus_tag="LCD50_RS24025"
                     /old_locus_tag="LCD50_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_247170839.1"
                     /translation="MKGNVAKPGRSFKNRVDYILKDDHDFICSNMSSDYNNVSDLTDEF
                     KIVSSFRQDIKKPVFHAFLSLPKNEHLTDEQWQEIAKDYLKEMNINIEKHQYICVRHND
                     TEQDHIHIVANRVGLDGSVWLGQHSAFNTIAACERLEVKHGLTITQSLKGQKSEVSAPT
                     KAEIEQALRTGEKPARLVLQNALQAALVGKPDLSTFVERLQAVGIDPAFNVASTGNVAG
                     VSFGIKNNEKNIYFKGSQLGKKYSWNTIKGKVKYDKNRDDELVRSFSARKDDEPNSIGR
                     SLAEPNINDSGAVDRINDDIIGVSQHSIKSDATDTNQSEQLIRSNNNRFKLSEKYNRPA
                     SHKVRRTTQKATQILNRVKKQHNKEPIKTYTKSSFNRGSNTFSGSSISRILDHAETANA
                     ATYSFRLKSGSRFNSRATKIEEIRSRLGIEKPVIQPKKIVKEEIKKKPLGLSIDENRFK
                     SKLKEALKKEKPKNETTPQQEDDQHLKPRGFKPR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1717..2085)
                     /gene="mobC"
                     /locus_tag="LCD50_RS24030"
                     /old_locus_tag="LCD50_24075"
     CDS             complement(1717..2085)
                     /gene="mobC"
                     /locus_tag="LCD50_RS24030"
                     /old_locus_tag="LCD50_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_193269750.1"
                     /translation="MKTDRKRGRIELNHEVRRDISIQFRVNKAEFEYLKELVEASGLER
                     GHYIRKEILNSEPVIKRKMPEFEQSLYREIQHQGNNINQIAKKLNTNEDFKQSLAAEAN
                     FAIKQLMKRILKACEVYV"
                     /name="mobC"
     gene            complement(2072..2341)
                     /locus_tag="LCD50_RS24035"
                     /old_locus_tag="LCD50_24080"
     CDS             complement(2072..2341)
                     /locus_tag="LCD50_RS24035"
                     /old_locus_tag="LCD50_24080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202162764.1"
                     /translation="MIMEYEMKLNVLARFFYYIEQAKDIPFYCSSYDEQSLCYFVANRY
                     INENKADDLIQALIDTNDDDYIKAIRDYVQYTALNEVRKKYEDR"
                     /name="hypothetical protein"
     gene            complement(2338..2511)
                     /locus_tag="LCD50_RS24040"
                     /old_locus_tag="LCD50_24085"
     CDS             complement(2338..2511)
                     /locus_tag="LCD50_RS24040"
                     /old_locus_tag="LCD50_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_165590481.1"
                     /translation="MIIAIGMMLLCIVLGGFMIRSCMGKTNSSSEAKQAISDVRKIIDK
                     KELIKYKRRNQR"
                     /name="hypothetical protein"
     gene            complement(2522..2779)
                     /locus_tag="LCD50_RS24045"
                     /old_locus_tag="LCD50_24090"
     CDS             complement(2522..2779)
                     /locus_tag="LCD50_RS24045"
                     /old_locus_tag="LCD50_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_147006712.1"
                     /translation="MKIVLSVSKCLYINDLDRLLPCDRFFVHLDYKDISKKESFEMNRH
                     DQESYKLIFDKLVGNDVMLAIDEDIYSEVIENLLYYSNKK"
                     /name="hypothetical protein"
     gene            complement(3397..3621)
                     /locus_tag="LCD50_RS24050"
                     /old_locus_tag="LCD50_24095"
     CDS             complement(3397..3621)
                     /locus_tag="LCD50_RS24050"
                     /old_locus_tag="LCD50_24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_247170837.1"
                     /translation="MHEQKDKKRRLGFMVGKSNATLPADEQTEAELDQEVLKLFYPNQC
                     TKDTIERSEAGTDVFTASGSDDLFKKLKM"
                     /name="hypothetical protein"
     gene            3919..4770
                     /locus_tag="LCD50_RS24055"
                     /old_locus_tag="LCD50_24100"
     CDS             3919..4770
                     /locus_tag="LCD50_RS24055"
                     /old_locus_tag="LCD50_24100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_247170838.1"
                     /translation="MFNIENNREILKSQIPLKPYATNDLEFGLTIQNKNKALDMLYLQV
                     NHPLYLHTMIFDLDKENCFYEFENAHLPIPSFITKSPESGRCHYGYMLNAPISSTEKSR
                     QKPVKFARALYYNMANRLGADLGYAGLITKNPLNPHWSPFWSGADLYELNDLADCFDDL
                     EEPKKRENTEFAFGRNVEMFDTIRQWAYKNVLKYQSESSFNDFHNELLLKCQMHNAYLN
                     ADDLLPYNEIKATAKSIAKFCWKEFSEEKLNKIQSKKQSYRGKKNKGIVKSKTRKFLEA
                     IK"
                     /name="replication initiation protein"
ORIGIN
        1 aaaagagtat gctgaacagt atgagattag cgttagaaca gtaaaaagat atttctctca
       61 agatcgtgaa gactatgaga aagaagcaaa acaaagacga ttaaaagcct ttgtattacg
      121 tgaaagcggg atgaaatggg cagatgtcgg tattgctctt ggaactacaa aacacggtgc
      181 tatagctctt tataaacgtt atcaacaaat agacgcacca aaagaagagg cttaaagcct
      241 cttatcttgg tttaaaaccg cgtggtttta aatgctgatc gtcttcctgc tgtggtgttg
      301 tttcattttt tggtttttct tttttaagag cttcttttaa cttcgattta aatctatttt
      361 catcaatact caaccccaaa ggctttttct ttatttcttc tttaactatt ttctttggct
      421 gaataactgg tttttctata ccaagtctac ttcttatttc ttcgatctta gttgctctac
      481 tattgaaacg gctaccgctc ttgagtctaa atgaataggt ggctgcgttt gctgtttctg
      541 catgatcaag tatcctactg atagaactgc cgctaaatgt attgctccca cgattaaagc
      601 tacttttagt ataagttttg attggttctt tattgtgttg ttttttaact ctgttgagta
      661 tttgtgtagc tttctgtgtt gttcttctaa ctttatggct tgctggtcta ttatattttt
      721 cgctgagttt aaagcggttg ttgttgcttc taatgagttg ttcactctgg ttagtgtctg
      781 ttgcgtctga tttaatgctg tgctgactga ctccgataat atcatcattg attctgtcaa
      841 cggctccact gtcattgata tttggttcag caagtgatcg tccaatacta tttggttcat
      901 catcttttct tgctgagaag ctcctaacga gttcatcatc tctatttttg tcatatttca
      961 ccttaccttt aatagtgttc caactgtatt ttttacctaa ctgtgagcct ttaaaataaa
     1021 tatttttttc attatttttg ataccaaacg acactccggc gacgttgcca gtgcttgcaa
     1081 cgttgaaggc cggatcgatg cctacagcct gcagacgttc tacgaacgta gacaaatcag
     1141 gcttaccgac taaagctgct tgtagtgcgt tctgtagcac taaacgggct ggtttctcac
     1201 ctgtcctcaa ggcttgctcg atctctgctt tggttggtgc tgatacctct gatttttgcc
     1261 cttttagact ttgtgtgatg gttaagccgt gctttacttc taaacgttca caagctgcta
     1321 ttgtgttaaa tgctgagtgc tgcccaagcc atacagaacc gtctaaaccg actctatttg
     1381 ctactatatg aatatggtct tgttctgtat cattatgacg tacacaaata tattgatgtt
     1441 tttctatatt aatattcatt tctttcaaat agtcttttgc tatctcctgc cattgttcat
     1501 ctgttaaatg ttcatttttt ggaagagata aaaaagcgtg aaaaactggt ttttttatat
     1561 cttgtctaaa gctacttaca attttaaact catcagttaa atcactgaca ttattgtaat
     1621 cacttgacat attgctacat atgaaatcgt ggtcatcttt aagaatgtaa tcaactctat
     1681 ttttaaatga tcttccgggt tttgctacat ttcctttcat acataaacct cacaagcttt
     1741 taatattctt ttcataagtt gctttatggc aaagttagct tctgcggcta aagattgttt
     1801 aaagtcttcg ttggtattta gttttttagc aatctgattt atattattac cttgatgttg
     1861 tatttctctg tataatgatt gctcaaactc aggcattttt ctttttataa cgggttcaga
     1921 gtttaaaatc tcttttctaa tgtaatgacc tctttcaagg ccggatgctt caacaagctc
     1981 ttttaaatat tcaaactctg ctttgtttac tctaaactgt atagatatat ctctacgtac
     2041 ttcgtgattt aactctatac gtcctctttt tctatctgtc ttcatatttc tttctcactt
     2101 catttaaagc tgtatactga acataatctc ttatagcttt tatataatca tcgtcattag
     2161 tatctatcaa tgcctgtata agatcatcag ctttattttc gttaatgtat ctattagcta
     2221 caaaataaca caagctttgt tcgtcatagc ttgaacaata aaaaggtata tctttagctt
     2281 gttctatgta ataaaaaaaa cgtgctaaca cattaagctt catttcatat tccataatca
     2341 tctttggttc cttcttttgt atttaataag ttctttttta tctattattt ttctaacatc
     2401 tgatattgct tgttttgctt cgcttgaaga gtttgttttt cccatgcagc ttctaatcat
     2461 aaaaccaccc aaaacaatac aaagaagcat cattcctatt gctataatca tatatgtctc
     2521 attatttctt atttgaataa taaagtaagt tttcgataac ttcgctgtag atgtcttcat
     2581 caatagcaag cattacgtca ttaccgacaa gcttatcaaa gataagtttg tagctttctt
     2641 gatcgtgtct gttcatctca aatgactcct ttttgctaat atccttataa tctaaatgaa
     2701 caaaaaaacg atcacacgga agtaagcgat ctaaatcgtt tatgtataaa cattttgata
     2761 ctgataaaac tattttcata tttattcctt ttggttttta agtgaactgt aaaactattt
     2821 accgccaccc ttgatagggt gattaggcgg aagggtagag ctttttcaac cttgcgagtg
     2881 aaacgagaca aggcaatccg ataggattgg gtaaaatagc ggatacgcta ttacaccttg
     2941 cctcagtttc cgaaaaacaa aaataaagct gaactcaacc tatatttaaa taaataaccg
     3001 atctaaaaag tttgtcaata tagcggctac ataaatagat acactgcaca acaaataaaa
     3061 tgcaaataaa aaatagggta aataatatat tgtcttgatg ggtagctgtg cgtaataggt
     3121 agctttgcgt aaaaaatgct gctgctacgt gaaatctgac aaagttcgtg accatcagat
     3181 caccagattt taaggaatgg ggtttgatcg gcggcgacag aacagtagaa aacttgcttt
     3241 tctgctgttg ctggcgtcta tgggtttaaa atgctcataa aatcaacaga ctatagtata
     3301 tattagtata tactgcatcg agggttggcc ttctctcacc gttccgggtc ggtttgggtt
     3361 tgttctactg gtgaagatgg ccagctctcc ccttccctac atcttcagtt ttttgaacag
     3421 gtcatcagaa ccggaagctg tgaaaacgtc tgtaccagct tctgagcgtt ctatggtgtc
     3481 tttggtgcat tggttaggat aaaacagctt caggacttcc tgatcaagct ctgcttctgt
     3541 ttgttcgtct gctggcaatg ttgcattgct cttaccaacc ataaaaccta accgccgttt
     3601 cttgtccttc tgttcgtgca tgatgtacct ccccagcaca gccagatcct gcatagagtt
     3661 tagtatacgt tacagtgtga cttactctgt agatggcctt cggcctgtgc ttcgcggttg
     3721 tatggtttta ctgaaaactc cgtctaatac ctgatattgg ttaatggcac acataacaac
     3781 tttttttgat ggatctattg acaagttttt ttggtatagt aaaactgtaa gagattataa
     3841 ataaaagaaa agcccgcgtt tcttggaaag tttggcgacc aacaagaaac ggggcattac
     3901 agaaaggatt actgctaaat gttcaatata gaaaataaca gagagatatt aaaaagtcaa
     3961 atacctttaa agccatatgc tacaaatgat ttagagtttg gattgactat tcaaaataag
     4021 aataaagctt tagatatgct ttatttacaa gtaaatcacc cgttgtatct tcatacaatg
     4081 atatttgatt tagacaaaga aaactgcttt tatgagtttg aaaatgctca tttgcctata
     4141 ccgtctttta taactaaatc accggaatct ggtagatgtc attacggtta tatgcttaat
     4201 gctccgatct catcaacaga gaaaagcaga cagaagccag taaagtttgc cagagcatta
     4261 tattacaata tggctaaccg tttaggtgct gatctggggt atgccggact gattacaaaa
     4321 aacccgctaa atcctcactg gtcgccgttc tggtcaggtg ctgatttata tgagttaaat
     4381 gatttagctg attgttttga tgatttagaa gaaccaaaga aaagagaaaa tacagagttt
     4441 gcttttggtc gtaatgttga aatgttcgac accataaggc agtgggctta taaaaatgtg
     4501 ctaaaatacc agagtgaaag ctcatttaat gatttccaca atgaactatt gctaaagtgt
     4561 caaatgcata atgcatattt aaatgctgat gatttactgc catataatga gataaaagcc
     4621 acagcaaaaa gcattgcaaa gttttgttgg aaagaattca gcgaagaaaa actcaacaag
     4681 atacagagta aaaaacagtc ttaccgtgga aagaaaaaca aaggaatagt aaaaagtaaa
     4741 actcgtaagt ttttggaggc cataaaatga aagttaaaaa tagaaaaaga gaacttaccg
     4801 c
//
