LOCUS       NZ_CP083860             5413 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Enterobacter kobei strain 12379-yvys plasmid p12379_3, complete
            sequence.
ACCESSION   NZ_CP083860
VERSION     NZ_CP083860.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21379548
            Assembly: GCF_023023705.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Liao,W., Cui,Y., Quan,J., Zhao,D., Han,X., Shi,Q., Wang,Q.,
            Jiang,Y., Du,X., Li,X. and Yu,Y.
  TITLE     High prevalence of colistin resistance and mcr-9/10 genes in
            Enterobacter spp. in a tertiary hospital over a decade
  JOURNAL   Int J Antimicrob Agents 59 (5), 106573 (2022)
   PUBMED   35307563
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Liao,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023023705.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 07:11:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,813
            CDSs (total)                      :: 4,697
            Genes (coding)                    :: 4,629
            CDSs (with protein)               :: 4,629
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 15 of 68
            Pseudo Genes (short protein)      :: 1 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083860.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="12379-yvys"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:208224"
                     /plasmid="p12379_3"
                     /geo_loc_name="China"
                     /lat_lon="30.15 N 120.10 E"
                     /collection_date="2012-05"
                     /collected_by="Yushan Cui"
     gene            complement(join(5316..5413,1..247))
                     /locus_tag="LCD50_RS23985"
                     /old_locus_tag="LCD50_24030"
     CDS             complement(join(5316..5413,1..247))
                     /locus_tag="LCD50_RS23985"
                     /old_locus_tag="LCD50_24030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_046380542.1"
                     /translation="MSEKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPAR
                     SGKLPSLSPALLRQLAGMGNNLNQIARRVNAGGDTGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGTDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1194..1520)
                     /locus_tag="LCD50_RS23990"
                     /old_locus_tag="LCD50_24035"
     CDS             complement(1194..1520)
                     /locus_tag="LCD50_RS23990"
                     /old_locus_tag="LCD50_24035"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_208644579.1"
                     /translation="MKLLDSNEVCELLGISKSTLYRWCGVSEDFSGLGLSSGLPQANAL
                     NSRLAKATQKSLGWENMLVGRALQQKALGVEETPSDFPRPFKIGRSFKWKDAEIMAWLE
                     TRRV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1793..2287)
                     /locus_tag="LCD50_RS23995"
                     /old_locus_tag="LCD50_24040"
     CDS             complement(1793..2287)
                     /locus_tag="LCD50_RS23995"
                     /old_locus_tag="LCD50_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000929317.1"
                     /translation="MAGTVWRDIRTGETVFPMGHLHPQRCCVDVNGTSVDIEISFGFHV
                     FTDEKQTGMLMKFKEEQRFFCRERYEGSKTIVHRILTAIENGEYITAFISKGQGQRYYH
                     LSHHDDFILMEIRKPQDRNNSLRIHVVTAYTLDEWGTVNKGRNLRFRYVLEQRLQGKKI
                     V"
                     /name="hypothetical protein"
     gene            complement(2288..2869)
                     /locus_tag="LCD50_RS24000"
                     /old_locus_tag="LCD50_24045"
     CDS             complement(2288..2869)
                     /locus_tag="LCD50_RS24000"
                     /old_locus_tag="LCD50_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_247170836.1"
                     /translation="MNNSLILLIFFNKLVSIDKRETTMSARARKAGKRNQSERRQDLIE
                     KLWEDDEIDTWHRLENDGYSTVPRYLPLIGDLMDGLSKGSPLSTTYLALWFRVSDEGLI
                     EIRDKAALAFESGFASERGVTTWAGRMKKLKELGFISCREGSTGEFHYVLIVHPLVAVK
                     KLLDEGIIPKGKTYNILSERVIEVGASWEG"
                     /name="hypothetical protein"
     gene            complement(3925..4140)
                     /locus_tag="LCD50_RS24005"
                     /old_locus_tag="LCD50_24050"
     CDS             complement(3925..4140)
                     /locus_tag="LCD50_RS24005"
                     /old_locus_tag="LCD50_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_086581678.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFEEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4147..4638)
                     /locus_tag="LCD50_RS24010"
                     /old_locus_tag="LCD50_24055"
     CDS             complement(4147..4638)
                     /locus_tag="LCD50_RS24010"
                     /old_locus_tag="LCD50_24055"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032623637.1"
                     /translation="MSSLLTLAKDLEQQSKARQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<4673..5377)
                     /locus_tag="LCD50_RS24110"
                     /old_locus_tag="LCD50_24060"
                     /pseudo=""
     CDS             complement(<4673..5377)
                     /locus_tag="LCD50_RS24110"
                     /old_locus_tag="LCD50_24060"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(342..515)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          482..987
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.095"
                     /coverage="99.0"
                     /evalue="1.71e-152"
     Typing          complement(3..226)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.333"
                     /coverage="98.0"
                     /evalue="6.73e-41"
     Typing          complement(3825..5326)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.094"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 catggccggt gtcgccgccg gcgttaaccc ggcgggctat ctgattcagg ttattgccca
       61 tgccggcaag ctggcgcagc agcgccggcg agagtgaggg gagctttccg gaacgtgccg
      121 gcttttcgtc caggcaggtc tgccgcatcc acgctgccag ctgcctgccg tcgcagcgct
      181 caagcagccg ccggtgctcg tcctccgtca cccacatggt gagcatcttg ttccgtttct
      241 ctgacaaacc ggcctcctga taacgccggg acgggcggga aagcctttcc cggtcggcgc
      301 ggggttggac aagccgcagg cgcgtcagtg gcttttagcg ggtttcgggg cgcagccctg
      361 aaccagtcac gcagcgctag cggagtgtat actggcttac tatgttggca cggatgcgaa
      421 tgtcagtgaa gtgcttcatg tggcgggaga aaaaaggcgg caccggtgcg tcagcagaat
      481 atgcgataca ggatatattc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      541 ctgcggcgag cggaaatggc ttacgaacgg ggcggaaatt tcctggtaga tgccaggaag
      601 agacttaaca gggaaacgat aaggccgcgg cgaagccgtt tttccatagg ctccgccccc
      661 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactta
      721 aagataccag gcgtttcccc ctggcggctc cctcttgcgc tctcctgttc ccgtcctgcg
      781 gcatccgtgt tgtggtggaa gctttaccta aatcaccacg tcccgttccg tttagacagt
      841 tcgctccaag ctgggctgtg tgcaagaacc ccccgttcag cccgactacc acgttgtccc
      901 ggtaactatc atcttgagtc caacccggaa cagacacgaa aaatcgccag tggcggtagc
      961 cattggtaaa caggatgtgc gagagatgta ttcagagttc ttgaagtggt ggccttagtg
     1021 aggctacact gaaaggacag atttggtgac acgtctcgca caagactgtt accacggtta
     1081 ttcaatctgc caggattaaa taaccttcga aaaaccacct gccagggtgg ttttttcgtt
     1141 tacaggacaa aagactacga cgagatccaa atgatctcaa gaagatctat caatcaaacg
     1201 cgtcgcgttt caagccaagc cataatttcg gcgtctttcc atttaaacga acgaccgatc
     1261 ttgaaaggac gagggaagtc gcttggcgtc tcttcgacac cgagagcttt ttgctgtaat
     1321 gccctcccaa cgagcatatt ttcccagcct aaagatttct gagtagcctt agccagccgg
     1381 ctattcaaag cgttagcctg aggaagaccg ctacttagac ccaagccaga aaaatcctca
     1441 gacaccccgc accaacgata caaagtggac ttactgatgc ctaagagctc acacacttca
     1501 ttactatcaa gcaacttcat atgaacacct ataaaaaaca atgaaaacct ataggcactt
     1561 ataaccccaa agatggtaaa tgacaaccta taaacatcaa tgaacaccaa aaagtacgct
     1621 agtttgacaa cgaacatgtg gaataggata ctaatgttgt cagcacaaac cggaacctcg
     1681 agagaggggt gctgatgccg ggggtcacac cctcccagcc tgtgagcggg ataacgaaag
     1741 gccagaccga aattctgcaa aaaacaaagg cgccttaggc gctttttgtt ttttacacaa
     1801 tcttctttcc ctgcagtcgc tgttccagca catatctaaa ccgcaggtta cgtcctttat
     1861 ttacagtccc ccattcatca agcgtgtaag ctgtaacaac atgaatacgc aaagaattat
     1921 ttctatcttg aggctttctt atttccatca aaatgaagtc atcatgatgg ctaagatggt
     1981 aataccgctg cccctgcccc tttgaaataa atgctgtaat gtattcaccg ttttctatcg
     2041 ctgtaagtat tctgtgaacg atagttttgc tgccctcata gcgttcccta caaaaaaacc
     2101 tctgttcctc cttaaatttc atcagcattc cggtttgttt ttcatcagta aaaacatgaa
     2161 aaccaaaact aatttctata tctacagaag taccgtttac atcaacacag cacctctgag
     2221 ggtgcaggtg ccccatagga aaaacagtct ctcccgttct tatgtcacgc catactgtgc
     2281 ccgccattta accctcccaa gaggctccca cctcaattac acgctccgac aaaatgttat
     2341 aagtctttcc tttagggata atcccttcat caagcagctt tttgactgca actaatgggt
     2401 gaacaattaa cacgtaatga aattcaccgg tgctgccttc acgacaggat atgaacccca
     2461 gttcttttag ctttttcatt ctccctgccc atgtagttac gcctctttca ctagcgaacc
     2521 ctgattcaaa agccagtgct gctttatctc ttatttcaat caaaccctca tcagaaaccc
     2581 tgaaccataa agccaagtag gttgttgata atggtgatcc ttttgacaga ccatccatga
     2641 gatcgcctat caaaggcaaa taacgtggaa ctgttgagta cccatcattt tcaagtcgat
     2701 gccaagtatc gatttcgtcg tcttcccaca atttttcaat caaatcctga cgtcgttctg
     2761 actgatttct cttacctgct tttcttgctc ttgcggacat agtcgtctcc cgtttgtcta
     2821 tggacactaa tttattaaaa aaaatcaata aaatcaaaga gttattcatg actggcagct
     2881 tgttctcatc tgaaaaaata gagttaaaaa caagctaaat cgcaataaac attacattta
     2941 aaaacataaa aagtaatata tattacaatg aagtttctta attatatgat taataatgat
     3001 ttacaagagg aaggcggtgc tcgttgtgct cggtgtgctc gatggttctc cctgtgctcc
     3061 ttgtgctcca tggggttgta gggggcaagc cagggcggat agagagaaaa ataatttagc
     3121 ggtagcgcgt ctcagagact aaaaaacccg ctgatgcggg ttttcttatt gttacaaaca
     3181 tcctcgccgt tgtagttcgg cttcaatcgc ctcacaagca gcgagtacaa tctcagtgtt
     3241 atagcaccag gctatgccac tgcggttaat actatcgatt aaggctcgat tatgagccaa
     3301 agtgcgtttt agggtggtgg tatctttgtc catcatcagg atctccattc aggttaacga
     3361 gcttcatgct cccagatatt tactcttgcc gttcctgaac aggctaaaac agcgaaaaaa
     3421 agcctatttt cgcttcatcc cagagcacca atatttacca taacatccgt tgcccgcacc
     3481 gctgaaacgg ggttggctgc gtttccggtg ccagacgcga gaaagtggcg acagacatga
     3541 cgaaaaaaca ccggaaacgc agccgctgga tattacactg ctgcatagtc atgcagccag
     3601 atgcatggcg cgtaaagtca cactaaaatc agcgcgaacg ccttaaaaac cggcaaatgc
     3661 ctctccagaa actcacacag ctccatcacc aggtaaaaac agttcagcag ggcttcaata
     3721 acgttgagaa ttttcatcac ttcctccggg ataagtcact accattccgg tagtcagcct
     3781 atttagcagg aacgcgatga gcccttcttc gtgcgggctg tcacctcatc ttcggaccat
     3841 cgaaactctt ctgcttctgt cggagcgtca gctccagctc gttctgacgc tgatactcca
     3901 gtacagcctt ctcctcgatg gcattcagtt cccggacaag cggcgcagct gctcgcttaa
     3961 actcagcacc tgcgtgctca agtccgacac gcgctcgttc agcatcgcgt tctcccgttg
     4021 cataagccca cacatgctcc gccattcctc gaaggcgctc tcccactcgt ccagcctttg
     4081 cgagtagtcc tgctgtagct gctctaatgc gctcagcaac tgcttttcca gctctgtcat
     4141 tttccgctac tcctgtttca ggcggatcca tgtcgtcccg tcgtaaggga tggcttcggt
     4201 ttgcatcccc ggcggcagga taagaaacct ccggccgtcg ctgctctcct gatagcgcac
     4261 tccccacgtc cgggcggtca ttttcgccag ggtatcttcc tgctgacgaa gatgcgtgta
     4321 attgtcggtt atctgctgcc cctgccacca cagaatgctc ccgctcgtgc cgatcagcag
     4381 cgctgacacc atcaggatgg tgagccatgt ccgcccggca gtccgcagta cgctccgccg
     4441 gtttttctcc atcgcctcgc tcatactctg ctcgtgggcg aggatggcgt cgctgattct
     4501 cctcgcgctt gcgctcagtt ccgctctgac agactgctcg tgctcgctga atgcggcttt
     4561 cagcatctcg ccggtactct gctgccgcgc tttcgattgc tgctctaagt ccttcgccag
     4621 cgttaaaaga ctgctcatag atggctccct tcagtcggat gtttcgcccc ccgtccgggt
     4681 cggcaatgct gatactgctt ttcgtggtgc gcaccacctc aaaacctgac cgttccagcg
     4741 cctcagtgac atcctgacgg cttttcagct ccccggacga agcaagggtc agcagtccgc
     4801 gcgtgatggc ttctgcggcc tcctgcttcg ttttcggcag tccggacggc gtgaccagcg
     4861 cccggcggtt ttccggtgcg ttcgggtcgt gcagccccag tctgccgttc acgatggtct
     4921 gccaggcatc gatgcgcggt cggtcggcgc ggtcgtagta cggctggagc cgcctgccgg
     4981 tcagcagctc ggtattgggg atcagaaagt tcagctccag acgccccttg tccgcgtgct
     5041 ccacccacag aatgctgtac tgatctttat cgagtccggg catcagaacc cgctcgaagc
     5101 tggccatcag cttttcccgc tggccgggag gcagatcggc ctccgcaaac gacaggacgc
     5161 cggaggtgta tttcttcacg taaggcgacg cgtcaatcag ctcccggact tcttccggct
     5221 tcccctgcag gacgctggcg ccttcacgct gccggtcttt acccagcaga tagtccaccg
     5281 gcccggcgcc accgccgcga ccgcgtggat gaaatttaac gatcatcgtc cgttcctttc
     5341 tcaagaacgg cgtgccgcag ccgctcaagc ccggcatcga tggccatcag cgccgccaca
     5401 atctgtaccc tgt
//
