LOCUS       NZ_CP083852             2494 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei subsp. steigerwaltii strain 14269-yvys
            plasmid p14269_3, complete sequence.
ACCESSION   NZ_CP083852
VERSION     NZ_CP083852.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21379550
            Assembly: GCF_023023665.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. steigerwaltii
  ORGANISM  Enterobacter hormaechei subsp. steigerwaltii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Liao,W., Cui,Y., Quan,J., Zhao,D., Han,X., Shi,Q., Wang,Q.,
            Jiang,Y., Du,X., Li,X. and Yu,Y.
  TITLE     High prevalence of colistin resistance and mcr-9/10 genes in
            Enterobacter spp. in a tertiary hospital over a decade
  JOURNAL   Int J Antimicrob Agents 59 (5), 106573 (2022)
   PUBMED   35307563
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Liao,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023023665.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:37:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,041
            CDSs (total)                      :: 4,926
            Genes (coding)                    :: 4,824
            CDSs (with protein)               :: 4,824
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 36 of 102
            Pseudo Genes (incomplete)         :: 62 of 102
            Pseudo Genes (internal stop)      :: 21 of 102
            Pseudo Genes (multiple problems)  :: 15 of 102
            Pseudo Genes (short protein)      :: 1 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083852.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Enterobacter hormaechei subsp. steigerwaltii"
                     /mol_type="genomic DNA"
                     /strain="14269-yvys"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="steigerwaltii"
                     /db_xref="taxon:299766"
                     /plasmid="p14269_3"
                     /geo_loc_name="China"
                     /lat_lon="30.15 N 120.10 E"
                     /collection_date="2014-04"
                     /collected_by="Yushan Cui"
     gene            1202..1393
                     /locus_tag="LCD40_RS25155"
                     /old_locus_tag="LCD40_25195"
     CDS             1202..1393
                     /locus_tag="LCD40_RS25155"
                     /old_locus_tag="LCD40_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021243041.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(1684..1869)
                     /locus_tag="LCD40_RS25160"
                     /old_locus_tag="LCD40_25200"
     CDS             complement(1684..1869)
                     /locus_tag="LCD40_RS25160"
                     /old_locus_tag="LCD40_25200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2235..2462)
                     /locus_tag="LCD40_RS25165"
                     /old_locus_tag="LCD40_25205"
     CDS             complement(2235..2462)
                     /locus_tag="LCD40_RS25165"
                     /old_locus_tag="LCD40_25205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          1550..1637
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(384..912)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 cactaaccat ctaactatag gtcatttttt atgtgaaaag aggcttgatg gtgggctgta
       61 tatcgagtta ggcgcgaggt gctatggtca aagtgtggtg tcaggcgggg aggaggctta
      121 tatgagatgt aatccggccg atacatatac caagatgatt taagtattcc tctttgtgag
      181 gttggatgtc taatttataa aggatcttct tgagatcctt tttttccgcg cgtaatctct
      241 tgccctgtaa acgaaaaaac caccctggca ggtggttttt cgaaggttag gtaatcctgg
      301 cagatcccct aaccgtggta acagtcttgt gcgagacatg tcaccaaatt tgtcctttca
      361 gtgtagcctc actaaggccg ccacttcaag aactcttgag acatctctcg cacatcctgt
      421 ttgccaatgg ccgttgccaa tggcgattag tcgtgtcttt ccgggttgga ctcaagttga
      481 tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccatc
      541 ctggagcgaa ctgccttccc ggaactgagt gtcaggcgtg gaatgagaaa ccgcggccat
      601 aacagcggag tgacaccggt aaaccgaaag gcaggaatgc gggggagcac gagggagcca
      661 ccagggggaa acgcctggta tctttaagcc gcatcgggtt tcgccaccac tgatttgagc
      721 gtcagattct gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttggcgcgg
      781 ccttatgctt tcttcgttaa gtatcttcct ggcatccccc aggaaatttc tgatccatcc
      841 gtaagcccgt cccgctcgcc gcagccgaac gaccgagcgg agcgagtcag tgagcgagga
      901 agcggaatat attctgtatc acattttctc ctgacgcgtt ttctttcact ttctgcgcct
      961 gtcttatgtg gcattaatgc tatgtgttac tgccatgcta catcttaagc cagtatacac
     1021 tccgctagtg ctccgtgact ggtccggcgc tgcgcccgga acccgcctgt accggttcag
     1081 cagccgttcc ggcctgactg caattttttt ttttcatccc tgcccgctac cctgtaaacc
     1141 tttcttctgc gttgccgtta acagtaggta agccatgttt ggtttttctt ccggagccct
     1201 gatgactctt agtgatgatg agcgacacct gcttgtgtcc gttgtttcgg tctggctacg
     1261 gcgtgccgga ggggatgccg gagccatgat gctggatgca taccggcaga ttttgtctga
     1321 aaccgaaccg gccgtaagga ctgtcatgct cgaatttctt gaatccgttc gcattcatta
     1381 catttcgtcc tgagcatttt ctgtatttct gtccatactt aaacgaggcg gccgtcacgg
     1441 acggtggtcg tcgttccatg atgctgttat ggacgagtga gagtaatcta atctaacttg
     1501 attgagttcc gggcgtgccg cccggttttt tttgctgttt cccggtgtcc gttccgggac
     1561 aggatgtgca tttgtaatac cgcgcacgcg ccggcatcac aaataccctg gtcagggctg
     1621 cgccccgaca ccccgccatc gcctcacggc cgcgcactga attgcgcacc cgttcaccga
     1681 ttttcacgca ttttcctcgc ctatctgtgc gttcagcatg gcgtaaagcg cttcggcctg
     1741 atcgtgcagg tgctcacagc agccggcagc ctcatccaga tccatctgct cgagccgctc
     1801 aaggaggaga agtgtctgtg cgcggataaa tttggccata ttgagggcgg cggattcctg
     1861 tctggtcact ggtgattttc cccgtctggt gatgattttt gcgtgaaaag agcatggcgt
     1921 cattctggtg catagtcatg catgccgtta aaatttaaca ggcgcgtttc tgacgggttc
     1981 cggggtggtt tgttgtggtt tttgtcatgg tttcgtcagg aacgcgctga acgcgtctca
     2041 ggcggtgcgg gcaacggatg ttatggtaaa ttcttggggg cttgattata aaataccgca
     2101 cttcctctta tgtcttgtgc tttttagaca accctgaaag cttttagctt tggtgatgca
     2161 atttgctggg attttggcgg agaaccacag gtaaagaaaa aggccacatt agcggccttt
     2221 ttcggagaag atgctcagtg cggaatttcg gagacttctt taacgtcggt tttattgatc
     2281 tgctgtttga caccattcgc gtctgtatag ccaagcaggc ctgagtctga ttctttaggt
     2341 ttgccgtcgc tgacaatagt gcgaccgtca ttggtgtgta tggcatagct tgtgcgcgtg
     2401 cagccggtga gggctgaaag cgcaactgcc gctgcgaaca ctgaaacaaa aatctttttc
     2461 aaagccagct ccttttatcc atatcagttt tggt
//
