LOCUS       NZ_CP083851             3115 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei subsp. steigerwaltii strain 14269-yvys
            plasmid p14269_2, complete sequence.
ACCESSION   NZ_CP083851
VERSION     NZ_CP083851.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21379550
            Assembly: GCF_023023665.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. steigerwaltii
  ORGANISM  Enterobacter hormaechei subsp. steigerwaltii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Liao,W., Cui,Y., Quan,J., Zhao,D., Han,X., Shi,Q., Wang,Q.,
            Jiang,Y., Du,X., Li,X. and Yu,Y.
  TITLE     High prevalence of colistin resistance and mcr-9/10 genes in
            Enterobacter spp. in a tertiary hospital over a decade
  JOURNAL   Int J Antimicrob Agents 59 (5), 106573 (2022)
   PUBMED   35307563
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Liao,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023023665.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:37:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,041
            CDSs (total)                      :: 4,926
            Genes (coding)                    :: 4,824
            CDSs (with protein)               :: 4,824
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 36 of 102
            Pseudo Genes (incomplete)         :: 62 of 102
            Pseudo Genes (internal stop)      :: 21 of 102
            Pseudo Genes (multiple problems)  :: 15 of 102
            Pseudo Genes (short protein)      :: 1 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083851.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Enterobacter hormaechei subsp. steigerwaltii"
                     /mol_type="genomic DNA"
                     /strain="14269-yvys"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="steigerwaltii"
                     /db_xref="taxon:299766"
                     /plasmid="p14269_2"
                     /geo_loc_name="China"
                     /lat_lon="30.15 N 120.10 E"
                     /collection_date="2014-04"
                     /collected_by="Yushan Cui"
     gene            join(2859..3115,1..1030)
                     /locus_tag="LCD40_RS25195"
     CDS             join(2859..3115,1..1030)
                     /locus_tag="LCD40_RS25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899670.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_234662132.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWERLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRGSAQNGASVIENS
                     ILMIDRIAQGMGAVSTDVSRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMITREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            2086..2322
                     /locus_tag="LCD40_RS25150"
                     /old_locus_tag="LCD40_25190"
     CDS             2086..2322
                     /locus_tag="LCD40_RS25150"
                     /old_locus_tag="LCD40_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063922967.1"
                     /translation="MTRLTNLTAAEKKFLDEAVAAAERVSGKKLNQQNRHIVLNRARAQ
                     IESQRHADRQRALHDEERQQADFTWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          1749..1929
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.561"
                     /coverage="84.0"
                     /evalue="1.26e-45"
     Typing          complement(1292..1766)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.797"
                     /coverage="92.0"
                     /evalue="1.2800000000000002e-163"
ORIGIN
        1 gcggctggca aagggaaatc gttccgtatg gcttgaagcc agttatatcc ccgtttctga
       61 caggcgcggc cgtgttatca gagtcattaa aattgctgcc gatatttctg agcgcgtgca
      121 ttctgctctt gagcaggaag cagtcgtaaa tgccataaac cgttctatgg ccatcatcac
      181 cttcaatcct gaagggatcg tgcttgaagc aaatgaaaat ttcgtcaatg ccactggcta
      241 taagcgggat gaaatcatag gtaagcatca tcgtctgttc tgtgccgaga cgctttacaa
      301 aagtgatgaa taccgacatt tctgggagag gctgaatcaa ggtgagtttt tttctggtct
      361 gtttccacgc cttaaccgtc agggagaccc tctgtggttc cgtgcaactt ataaccctgt
      421 ctttaacagt gatgggcagc tttataaaat tgtgaaattc gctacagatg tcacggctga
      481 tgtcctgcga aaccagagag agcaggaggc tgctgtgcat gcatgggata tggccgtgca
      541 gacccgagga agtgcgcaga acggtgccag tgttattgaa aatagcatcc ttatgattga
      601 cagaattgcg caggggatgg gggctgtctc caccgatgtc tcacggctca acaatcagtc
      661 tgaaagcatt gacgatatgg tggaaaccat ccggaagttt gccatgcaga caagacttat
      721 tgcactgaat gccgcgattg aagcagcaag ggctggcgca tccggaagaa gttttgcggt
      781 tgttgccgca gaagtgcgta atcttgcggc gagcgtcagt agcgcgactg aagaaattga
      841 gcaagtcgtg gccagcaata gtcaactggc caaggatgtt ctctgtggca ttgaaaacag
      901 cctgatgatc actcgggaag gggttacact catgcgcgaa gcgggtgaag ttatgaccag
      961 cattcagagg aatgctgcag gggttgagac tgcggttaag gatgtcgcca tttcagttaa
     1021 agccggatag gccatggtgc atatccccat atctgtcgaa catcacagat gatcttcttg
     1081 agatcctttc cgttgcgtcg taatctcttg ctctgaaaac gaaaaaacca cctggggagg
     1141 tggtttttcg aaggttcagc aagttgggga acttctgaac cgtggtaaca gggtgtacaa
     1201 gaccgctgcc accaaattcg tcctttcagt ttagccgtag ttaggcttca acttcaagaa
     1261 ctctgcacca gttatctctt gtacacccct ttaccagtgg ctaccgccaa tggcgatttg
     1321 tcgtgccttt tcgggttgga ctcaagacga tagttaccga aaagagcgtg gtagtcggac
     1381 tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt
     1441 gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa
     1501 ggcaggaaca ggagagcgca cgagggagct accaggggga aacgcctggg atctttatag
     1561 tcctgtcggg tttcgccgcc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg
     1621 gcggagccta tggaaaaacg gcaacgccgc atcctttttt tctcctgatt ttagcgccac
     1681 tctttctctt gtcattcgtg agctggttac gcccgccaca gtctcacggc cggagcgtag
     1741 cgaccgagtg agcgaggagg cgtcatttca tcgctaccag tattttgcac ttacgccatg
     1801 cagctacatt ttcccttgcc atatgaagca ctttcgtgat tccttcctgc gggctaacat
     1861 attaagccag tatacactcc gctagcgcta tgtgactggt tcagggctgc gccccgaaac
     1921 ccgctaacag ccactgacgc gcctgcggct tgtccaacgc ctcacgatac cggattaaaa
     1981 tccgctcccg ttcaccgttt ctgtcgacac aggaggctcg ccggcgcttc atcgatttcg
     2041 ccgtgtctgg cagactgtgc agcattattc tgaccggaga actacatgac ccgactgacc
     2101 aacctgacgg ccgccgagaa aaaatttctc gacgaggctg ttgccgccgc tgagagagtg
     2161 tcaggcaaga agctaaatca gcagaaccgg catatcgtcc tgaaccgggc gcgggcgcag
     2221 atcgaatcgc agcgacatgc tgaccgtcag cgggcgttac atgatgaaga acggcagcag
     2281 gcggatttta catggtcacg tccgcgtgcg ccgcgccgct gatcgtagat ttcggtgggt
     2341 tcgtggcatt gatgccgcga aacgagcctc cattcacctg cgtgcatagc catgcaggtg
     2401 cgcagaaatc atcaggagtg tttttcagcg atacagacgg ggaatggtgc cggaaatggc
     2461 cctcaggccg cagaaacgcg ctgagggcgt cccgggcggt gcgggcaaga actcttatgt
     2521 taaataagac gctccgggca gctaagatga aacccggcag gcttctcagt gccgccttgc
     2581 ttgtagcgcg atgcatttct ttccagaatt aaggggaccg gttcctgctg gttccggtcg
     2641 ggtaacaatt tcgaatctta tctctggttc accccgctaa agatggcggg ttttttacgc
     2701 gtgaaatccg gagccggaca tcatgccggc atggctgaag agcgggcttt ttcccgccct
     2761 gtcgcagtaa tatataaagc tacataagga gtagccgata accatggcag tacttacatt
     2821 atccgggcaa tctgattcag atgttttcac ttaaatatat gttctccctg aacctccagc
     2881 gatcagccat ttcgtatgac gcaatttgtc agaataagcc tgtcattgag ttcagtccgg
     2941 agggggtgat aaataaagcc agcccgcttt ttctctccac tatggggtac agggctgatg
     3001 aaataatcgg tcaccaccat cgtatattct gcccgccctc gctggtaagt tcaccggaat
     3061 acagccagtt ctggcagcgc cttgccaggg gagaaagctt tagtggcaag tacct
//
