LOCUS       NZ_CP083848             4938 bp    DNA     circular CON 07-APR-2024
DEFINITION  Enterobacter asburiae strain 16773-yvys plasmid p16773_6, complete
            sequence.
ACCESSION   NZ_CP083848
VERSION     NZ_CP083848.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21379551
            Assembly: GCF_023101705.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Liao,W., Cui,Y., Quan,J., Zhao,D., Han,X., Shi,Q., Wang,Q.,
            Jiang,Y., Du,X., Li,X. and Yu,Y.
  TITLE     High prevalence of colistin resistance and mcr-9/10 genes in
            Enterobacter spp. in a tertiary hospital over a decade
  JOURNAL   Int J Antimicrob Agents 59 (5), 106573 (2022)
   PUBMED   35307563
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Liao,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023101705.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 09:04:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,193
            CDSs (total)                      :: 5,079
            Genes (coding)                    :: 4,881
            CDSs (with protein)               :: 4,881
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 120 of 198
            Pseudo Genes (incomplete)         :: 80 of 198
            Pseudo Genes (internal stop)      :: 20 of 198
            Pseudo Genes (multiple problems)  :: 21 of 198
            Pseudo Genes (short protein)      :: 1 of 198
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083848.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="16773-yvys"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:61645"
                     /plasmid="p16773_6"
                     /geo_loc_name="China"
                     /lat_lon="30.15 N 120.10 E"
                     /collection_date="2016-07"
                     /collected_by="Yushan Cui"
     gene            join(4859..4938,1..1207)
                     /locus_tag="LCD43_RS25930"
                     /old_locus_tag="LCD43_25965"
     CDS             join(4859..4938,1..1207)
                     /locus_tag="LCD43_RS25930"
                     /old_locus_tag="LCD43_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_306170312.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            2216..2539
                     /locus_tag="LCD43_RS25935"
                     /old_locus_tag="LCD43_25970"
     CDS             2216..2539
                     /locus_tag="LCD43_RS25935"
                     /old_locus_tag="LCD43_25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007899679.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTAKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGVGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2748..>3182
                     /locus_tag="LCD43_RS25940"
                     /pseudo=""
     CDS             2748..>3182
                     /locus_tag="LCD43_RS25940"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3217..3708
                     /locus_tag="LCD43_RS25945"
                     /old_locus_tag="LCD43_25980"
     CDS             3217..3708
                     /locus_tag="LCD43_RS25945"
                     /old_locus_tag="LCD43_25980"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_016355729.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESNDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            3715..3930
                     /locus_tag="LCD43_RS25950"
                     /old_locus_tag="LCD43_25985"
     CDS             3715..3930
                     /locus_tag="LCD43_RS25950"
                     /old_locus_tag="LCD43_25985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1929..2101
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.14e-86"
     Typing          complement(1458..2004)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.545"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2217..2533
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="3.03e-59"
     Typing          2530..4031
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.692"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggagggggtg ataaataaag ccagcccgct ttttctctcc actatggggt acagggctga
       61 tgaaataatc ggtcaccacc atcgtatatt ctgcccgccc tcgctggtaa gttcaccgga
      121 atacagccag ttctggcagc gccttgccag gggagaaagc tttagtggca agtacctgcg
      181 gctggcaaag ggaaatcgtt ccgtatggct tgaagccagt tatatccccg tttctgacag
      241 gcgcggccgt gttatcagag tcattaaaat tgctgccgat atttctgagc gcgtgcattc
      301 tgctcttgag caggaagcag tcgtaaatgc cataaaccgt tctatggcca tcatcacctt
      361 caatcctgaa gggatcgtgc ttgaagcaaa tgaaaatttc gtcaatgcca ctggctataa
      421 gcgggatgaa atcataggta agcatcatcg tctgttctgt gccgagacgc tttacaaaag
      481 tgatgaatac cgacatttct gggagagtct gaatcaaggt gagttttttt ctggtctgtt
      541 tccacgcctt aaccgtcagg gagaccctct gtggttccgt gcaacttata accctgtctt
      601 taacagtgat gggcagcttt ataaaattgt gaaattcgct acagatgtta cggctgatgt
      661 cctgcgaaac cagagagagc aagaggctgc tgtgcatgca tgggatatgg ccgtgcagac
      721 ccgagaaagt gcgcagaacg gtgccaatgt tattgaaaat agcatcctta tgattgacag
      781 aattgcgcag gggatggggg ctgtctccac cgatatctca cggctcaaca atcagtctga
      841 aagtattgac gatatggtgg aaaccatccg gaagtttgcc atgcagacaa gacttattgc
      901 actgaatgcc gcgattgaag cagcaagggc tggcgcatcc ggaagaagtt ttgcggttgt
      961 tgccgcagaa gtgcgtaatc ttgcggcgag cgtcagtagc gcgactgaag aaattgagca
     1021 agtcgtggcc agcaatagtc aactggccaa ggatgttctc tgtggcattg aaaacagcct
     1081 gatgaacact cgggaagggg ttacactcat gcgcgaagcg ggtgaagtta tgaccagcat
     1141 tcagaggaat gctgcagggg ttgagactgc ggttaaggat gtcgccattt cagttaaagc
     1201 cggataggcc atggtgcata tccccatatc tgtcgaacat cacagatgat cttcttgaga
     1261 tcctttccgt tgcgtcgtaa tctcttgctc tgaaaacgaa aaaaccaccc ggcaaggtgg
     1321 ctttttcgaa ggttcgcaag agttgacgct cttttgaacc gcggtaactg gcttggagga
     1381 gcacagtcac caaaactgtc ctttcagtgt agccttatcg cacccgaaac ttcaagactc
     1441 tcctcctcta aacctgttac cagtggctgc tgccagtggc gctttgtcgt gtttttccgg
     1501 attggactca agttgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc
     1561 gtgcatacag tccagcttgg agcgaactgc cttcccggaa ctgagtgtca ggcgtggaat
     1621 gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag
     1681 agcgcacgag ggagccacca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1741 gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga
     1801 aaaacggctt cgccgcggcc ttatcgcttc cctgttaagc ttctttctgg catctcccag
     1861 gaaatttccg ccccgcctgt aagccggtac cgctcgccgc agccgaacga ccgagcgcag
     1921 cgagtcagtg agcgaggaag cggaatatat cctgtatcgc atattctgct gacgctccgc
     1981 tgctgcattt ttctcctgcc acatgaagca cttcactgat attcgcatcc gtgccaacat
     2041 agtaagccag tatacactcc gctagcgcta cgtgactggt tcagggctgc gccccgaaac
     2101 ccgctaacag ccactgacgc gcctgcggct tgtccaaccc cgcgccgacc gggaaaggtt
     2161 ttcccgcccg tcccggggtt atcaggagac tgttttgtca gagaaacgga acaagatgct
     2221 caccatgtgg gtgacggagg atgagcacag gcggctactt gagcgctgcg acggcaggca
     2281 gctggcggcg tggatgcggc agacctgcct ggacgaaaag ccggctcgta ccgcaaaact
     2341 tccttcgatc tcgccggcgc tgctgcgcca gcttgccggt atgggcaaca acctgaacca
     2401 gatagcccgc cgtgttaacg ccggcgtcgg taccggtcat gaccgggtgc agattgtggc
     2461 ggcgctgatg gccatcgatg ccgggcttga gcggctgcgg cacgccgttc ttgagaaagg
     2521 aacggacgat gatcgttaaa tttcatccac gcggtcgcgg cggtggcgcc gggccggtgg
     2581 actatctgct gggtaaagac cggcagcgtg aaggcgccag cgtcctgcag gggaagccgg
     2641 aagaagtccg ggagctgatc gacgcgtcgc cttacgtgaa gaaatacacc tccggcgtcc
     2701 tgtcgtttgc ggaggccgat ctgcctcccg gccagcggga aaaactgatg gccagcttcg
     2761 agcgggttct gatgcccgga cttgataaag atcagtacag cattctgtgg gtggagcacg
     2821 aggacaaggg gcgtctggag ctgaactttc tgatccccaa taccgagctg ctgaccggca
     2881 ggcggctcca gccgtactac gaccgcgccg accggccgcg tatcgatgcc tggcagacca
     2941 tcgtgaacgg cagactgggg ctgcacgatc cgaacgcgcc ggaaaaccgc cgggcgctgg
     3001 tcacgccggc cggactgccg aaaacgaagc aggaagccgc agaagccatc acccgtggac
     3061 tgctgagctt agcctcgtcc ggggagctga aaagccgtca ggacgtcact gaggctcttg
     3121 aaggtgcagg ttttgaggtg gtgcgtacaa cgaaaagcag tatcagcatt gccgaccctg
     3181 acggggggcg aaacatccga ctgaagggag ccatctatga gcagtctttt aacgctggcg
     3241 aaggacttag agcagcaatc gaaagcgcag cagaagagta ccggcgagat gctgaaagcc
     3301 gcattcagcg agcacgagca gtctgtcaga gcggaactga acgcaagcgc gaggagaatc
     3361 agcgacgcca tcagcgccca cgagcagagt atgagcgagg cgatggagaa aaaccggcgg
     3421 agcgtgttgc tgacggccgg gaggacatgg ctcaccatcc tgatggtgtc agcgctgctg
     3481 atcgccacga gcgggagcat tctgtggtgg cagggacggc agataaccga caattacaca
     3541 catcttcgtc agcaggaaga taccctggcg aaaatgaccg cccggacgtg gggagtacgc
     3601 tatcaggaga gcaacgacgg ccggaggttc cttatcctgc cgccggggat gcaagccgaa
     3661 gccatcccct acgacgggac gacctggatc cgcctgaaac aggagtagcg gaaaatgaca
     3721 gagctggaaa agcagttgct gagcgcatta gagcagctac agcaggacta ctcgcaaagg
     3781 ctggacgaat gggagagcgc cttcgcggaa tggcggagca tgtgtggact tatgcaacgg
     3841 gagaacgcgg tgctgaacga gcgcgtgtcg gacttgagca cgcaggtgct gagtttaagc
     3901 gagcagctgc gccgcttgtc cgggaactga atgccatcga ggagaaggct gcactggagt
     3961 atcagcgtca gaacgagctg gagctgacgc tccgacagaa gcagaagagt ttcgatggtc
     4021 cgaagatgag gtgacagccc gcacgaagaa gggctcatcg cgttcctgct aaataggctg
     4081 actaccggaa tggtagtgac ttatcccgga ggaagtgatg aaaattctca acgttattga
     4141 agccctgctg aactgttttt acctggtgat ggagctgtgt gagttgccgg agaggcattt
     4201 gccggttttt aaggcgttcg cgctgatttt agtgtgactt tacgctgcat gcatctggct
     4261 gcatgactat gcatcagtgt aatatccagc ggctgcgttt ttggtgtttt ttcgtcatgt
     4321 ctgtcgccac tttctcgcgt ctggcaccgg aaacgcagcc aaccccgttt ctgcggtgcg
     4381 ggcaaggggt gttatgttaa aagccgaggt ctggcggata cggaaaaaat tatgccggcc
     4441 gggcttatgc ccgcggcaat ccggccgata cttaaattat ccttcacaaa ggatgtgctg
     4501 cgatccaggc agttgagtgt tttccggctc ccggatgtga gccatttttt tgccttctga
     4561 tgcctgttaa tgcatgcgga gctgcgctgg ttcttccaag attgatagac gccagcttct
     4621 ctgctggagc cggcggattt gccgtaaata tgaacatttt cttattgttc agcccgctaa
     4681 agatggcggg ttttttacgc gtgaaatccg gagccggaca tcatgccggc atggctgaag
     4741 agcgggcttt ttcccgccct gtcgcagtaa tatataacgc tacataagga gtagccgata
     4801 accatggcag tacttacatt atccgggcaa tctgattcat atgttttcac ttaaaaatat
     4861 gttctccctg aacctccagc gatcagccat ttcgtatgac gcaatttgtc agaataagcc
     4921 tgtcattgag ttcagtcc
//
