LOCUS       NZ_CP083841             3497 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Enterobacter asburiae strain AR0468-yvys plasmid pAR0468_7, complete
            sequence.
ACCESSION   NZ_CP083841
VERSION     NZ_CP083841.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21379552
            Assembly: GCF_023023265.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Liao,W., Cui,Y., Quan,J., Zhao,D., Han,X., Shi,Q., Wang,Q.,
            Jiang,Y., Du,X., Li,X. and Yu,Y.
  TITLE     High prevalence of colistin resistance and mcr-9/10 genes in
            Enterobacter spp. in a tertiary hospital over a decade
  JOURNAL   Int J Antimicrob Agents 59 (5), 106573 (2022)
   PUBMED   35307563
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Liao,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023023265.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 03:55:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,365
            CDSs (total)                      :: 5,251
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 43 of 177
            Pseudo Genes (incomplete)         :: 127 of 177
            Pseudo Genes (internal stop)      :: 36 of 177
            Pseudo Genes (multiple problems)  :: 24 of 177
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083841.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="AR0468-yvys"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:61645"
                     /plasmid="pAR0468_7"
                     /geo_loc_name="China"
                     /lat_lon="30.15 N 120.10 E"
                     /collection_date="2018-04"
                     /collected_by="Yushan Cui"
     gene            complement(1..435)
                     /locus_tag="LCD48_RS26765"
                     /old_locus_tag="LCD48_26775"
     CDS             complement(1..435)
                     /locus_tag="LCD48_RS26765"
                     /old_locus_tag="LCD48_26775"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_246917720.1"
                     /translation="MVVDLFSRKVIGWSMQPRMTKDIVLNALLMAVWRRNPQKQVLVHS
                     DQGSQYTSYEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEA
                     RGDIFDYIEMFYNSKRRHGSSDHMPPTEYEKQYYQRLGSV"
                     /name="IS3 family transposase"
     gene            complement(558..2066)
                     /gene="ltrA"
                     /locus_tag="LCD48_RS26770"
                     /old_locus_tag="LCD48_26780"
     CDS             complement(558..2066)
                     /gene="ltrA"
                     /locus_tag="LCD48_RS26770"
                     /old_locus_tag="LCD48_26780"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_033147074.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAHT
                     PGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALRDR
                     IVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEGDLS
                     SYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVISPLL
                     SNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCRYADDF
                     VLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIRKRSRYG
                     EMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQFVDFKAKV
                     FSYIDRVVFWKLAHWLARKYRTGIASLMRCWCKSPKPGQSKTWVLFGKTNHGKLSGEIL
                     YRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
                     /name="ltrA"
     gene            complement(<2739..3415)
                     /locus_tag="LCD48_RS26775"
                     /old_locus_tag="LCD48_26785"
                     /pseudo=""
     CDS             complement(<2739..3415)
                     /locus_tag="LCD48_RS26775"
                     /old_locus_tag="LCD48_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611921.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
ORIGIN
        1 ctagacactt ccgagccgct gataatattg tttttcgtat tcagttggtg gcatatgatc
       61 actcgaacca tgccgacgct tactgttata aaacatttcg atgtaatcaa aaatatcacc
      121 gcgagcttct tctcgcgttc cgtagatctt tttctttatc cgttcacgtt tcagtaactg
      181 gaaaaaactt tctgcgactg cgttgtcatg acagttaccg cgacggctca tactgccctc
      241 cagcccgtgt gatttcagga acgactgcca ctcatagctt gtgtactgac tgccttgatc
      301 agaatgaacc agcacctgct tttgaggatt acgtcgccac acggccatca gaagtgcatt
      361 caggacaata tcctttgtca tccggggctg cattgaccag ccaataactt tgcgcgagaa
      421 caggtcaaca accacagcca gatacagcca gccttcgtga gtccggagta gagtaagagg
      481 cagggcgtag tcggactatt tccctgcctc tcctccccga accggacgtg cacctttcag
      541 cgcatccggc tctccattta aatgctggcg aacgccattg ccacttctgt aaagcgcgat
      601 gtatacgtgt ttctggtctc cgtcctcaga tagggattac cttcgggtag ccgccagcgg
      661 aacagcttct tgccttgccc caccaaccgg tacagtattt cgccgctgag cttgccgtga
      721 ttggttttac caaataaaac ccacgttttg ctctgacccg gtttcggtga tttacaccaa
      781 cacctcatca gggaagcgat acctgtacgg tatttgcggg ccagccagtg agccagcttc
      841 cagaacacga cacggtcgat ataactgaag actttggcct taaaatcaac gaactgatag
      901 aacatggccc agcctttcag ttttcggttg agttgttcag ccatatcgac tttgctttca
      961 ctgtagttgc ctgataacag tgccgtcagc gatgcggcga agtttctggc tttctcctgc
     1021 gggatcgttg agaccactcg catctcgcca taacgactgc gtttgcgaat gatcctgtgc
     1081 cccagaaaga taaagccgtc attaacatgg gtgattttag tcttatccat gttcagcctg
     1141 agtttcagac tgccttcgag cacaccccga cactcctccc tgatggcttc cgcctgtgct
     1201 ttggtgcctt tgacgatgag gacaaaatca tcggcatagc ggcagtacgc caccgcgggt
     1261 ttccactgcc agttttctct gaccgccgta cttcggcccc gttggatact gttattccag
     1321 taccaccgat cttttctggc tttcccgctc aggtagcgct catgcaggta ttgatcgaac
     1381 tcattcagca tgatgttcga taatagcggc gatataacac cgccctgtgg tacaccttca
     1441 ctggccgccc gaaagagacc gacatcgata tgtcccgcct tgatggtttt ccacagcaga
     1501 gtcatgaaac gtgcgtcact gatcctgcgg cgtacagcct tcatcagcag tcgatgatgt
     1561 acggtgtcga agtaactgga caggtcgcct tcaatcaccc agcgtccccg ggtttcacca
     1621 cagtctgtga gctgtaattt caccgtgcgg atcgcgtggt ggacactgcg ctcaggccgg
     1681 aagccatatg agagcgtatg aaaatcactc tcccatatcg gctccatcgc catcagcatg
     1741 gcccgctgaa caatacgatc ccgcaacgcg gggataccca gtggtcgcag tttgccgttg
     1801 cttttaggga tgtaaacccg tctggcgggc aagggctggt agtggcctga gagtaattca
     1861 tccctgagga tttgcagctc aacagccagt ctggcctgta gcattgtttt gttcacgcca
     1921 tcaacgccgg gggtatgggc cccctttgat gaaagcgtga tccgcgccgc ttcagccagc
     1981 cattctggtt gtgttatcag acgcagcagc cgttgaatcc gtagggacgg atcggtggct
     2041 gcccatgtgg caagcttgcg ttgcatttcg ctgattatca aaggtcttca cctcgttagg
     2101 tcagttaatt cacgtcgcaa acacattcaa actgcttccc ttcgccatgt aatgggcttt
     2161 ccccatcgcg gactactacg gaagctccgc cagccagcgc gtcatcggag ccatgccccc
     2221 ttaacatccg tcgctgacct tccccggttt acctgcctgg actcaggcat actgaggagg
     2281 ctgcccgtcg cactctttat ccttgcttgc cgcaagttgg cagaagtcag caacgcaagc
     2341 gtgatagacg ctgctgcccc ggtgtttcgc atacatgtca aaacaccttc gaccggcagt
     2401 gcttacgtat cactgccagt tcctcctgca cggcctgtca gatcacgtag gccgtggtga
     2461 cgttttcaac ccacagaggc ggattaacgg gttcatgttc ttcagccttt cagtacttaa
     2521 ccttgaggat catctcggct tagtgatctc gcctcaatcc ccgttgtcag cgggttacat
     2581 caccctgcgg gcatgccgca ggtcactgcc gctcaggttc tccaccgtca cacccggtgg
     2641 gattgttggg tttctcatcg tgagttaccg gttcaatatt ccagacagac tcgcggttca
     2701 tttaagcatc catgcccgcc ctgaactccg ggcacactgt aggtgatgtc cgttacccaa
     2761 cgctcatccg gtgcttccgg gttgaactgc cgctggagcc tgttgggcgt cacgatgctg
     2821 gtttcaccct tacgtgcccg tgggctacgg tacccgacct gagcccttat cccggcacgc
     2881 ttcatcagtc gccagacacg gttaactccg cactgttgtc cggtatcccg caggtcgagg
     2941 tggatcttgc gataaccata aacgcaaccc gactccagcc agaactgctt tatcagcccc
     3001 gtcagtctca ggtcagtatg atgccgccgt gaatccggct gctgaagcca ggcgtaaaaa
     3061 ccactcggat gcacatccag cacccgacaa agcagtcgga caggccagca atgggtgttg
     3121 tcacggataa aggcgtacct cagtcggaca gctttgcgaa gtacgccgct ttttttaata
     3181 tgtcccgttc gtcggtaacc cgcttcagct ctttctggag gcgtcggatc tcagcctgag
     3241 cgtctgactg gactttattg gttgaggaat ccgggccata tgcctttatc caggcataaa
     3301 gactgtgggt ggtgataccg agacgtgttg ccacgctgga aacagaatgg ccgcgatcaa
     3361 caacctgttt taccgcttca attttaaact cttcgggata acgcttaccg ctcatgggca
     3421 cctctcttta agtcatctta aatgactctg aggtgtctgt taaacccgtg gcgattcaga
     3481 ccttgccatc acgactg
//
