LOCUS       NZ_CP083840             3995 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Enterobacter asburiae strain AR0468-yvys plasmid pAR0468_6, complete
            sequence.
ACCESSION   NZ_CP083840
VERSION     NZ_CP083840.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21379552
            Assembly: GCF_023023265.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Liao,W., Cui,Y., Quan,J., Zhao,D., Han,X., Shi,Q., Wang,Q.,
            Jiang,Y., Du,X., Li,X. and Yu,Y.
  TITLE     High prevalence of colistin resistance and mcr-9/10 genes in
            Enterobacter spp. in a tertiary hospital over a decade
  JOURNAL   Int J Antimicrob Agents 59 (5), 106573 (2022)
   PUBMED   35307563
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Liao,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023023265.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 03:55:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,365
            CDSs (total)                      :: 5,251
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 43 of 177
            Pseudo Genes (incomplete)         :: 127 of 177
            Pseudo Genes (internal stop)      :: 36 of 177
            Pseudo Genes (multiple problems)  :: 24 of 177
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083840.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="AR0468-yvys"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:61645"
                     /plasmid="pAR0468_6"
                     /geo_loc_name="China"
                     /lat_lon="30.15 N 120.10 E"
                     /collection_date="2018-04"
                     /collected_by="Yushan Cui"
     gene            complement(join(3512..3995,1..101))
                     /locus_tag="LCD48_RS26740"
                     /old_locus_tag="LCD48_26750"
     CDS             complement(join(3512..3995,1..101))
                     /locus_tag="LCD48_RS26740"
                     /old_locus_tag="LCD48_26750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_246917714.1"
                     /translation="MYENYTRAQQVLDVLEKTCRCIRANRGIILTRILNRKGLMTEPSI
                     AYLIDSLRPKEAKRFCEAMSFIEGHMSRLNTEDKAFVRTEAPATKGFYMAYLQDHPTVI
                     ELARRMMIGERRFTDVDPQLIDVASNLASSPTWCVKVLIPSIDPRKAAIIISNLTAWIY
                     QALERLKGKPAPWQYVNQLKKMSYELQQMRC"
                     /name="hypothetical protein"
     gene            231..623
                     /locus_tag="LCD48_RS26745"
                     /old_locus_tag="LCD48_26755"
     CDS             231..623
                     /locus_tag="LCD48_RS26745"
                     /old_locus_tag="LCD48_26755"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_063617155.1"
                     /translation="MASHSVTEAAKLAGVTRRTIYRHIKAGKLSASVTSSDNTVIETSE
                     LLRVYGVLSQPEPEMVSTGSHENQPEYVTLLLAEMSQLREQISTLASKVDELQERLALP
                     SPTKTDQSVLDWVSERRRLNKKNRAK"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1052..1684)
                     /locus_tag="LCD48_RS26750"
                     /old_locus_tag="LCD48_26760"
     CDS             complement(1052..1684)
                     /locus_tag="LCD48_RS26750"
                     /old_locus_tag="LCD48_26760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716169.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_063617154.1"
                     /translation="MRTYAYLRASTAEQDAARASQSLHTFAEEHGLTIAANFIENVSGA
                     SLQRPELFRLLAVAQAGDILLVEQIDRLSRLDADDWERLRGVIQSKGIRIVALDLPTSY
                     LQLQSGNDEFMRSMMNAINGMMLDMLAAIARKDYTDRRRRQTQGIEKAKEMGLYKGRQP
                     DLEKHEHIRTLIAQGNSQRKVAKMLGCAVGTVRNALNAEEKLDGHST"
                     /name="recombinase family protein"
     gene            complement(2319..3236)
                     /locus_tag="LCD48_RS26755"
                     /old_locus_tag="LCD48_26765"
     CDS             complement(2319..3236)
                     /locus_tag="LCD48_RS26755"
                     /old_locus_tag="LCD48_26765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_084833449.1"
                     /translation="MLLKPAALRTDGRTYEVFLPYLIDKLPERQRKRPAQWFQAALTNL
                     LGAPTEQQETCTPELISGEWDHHTDWISAFDALALLSNSPDSYQNDDLINEIEDKTGVK
                     LPVMSGKSKHEAVFGRRLIGVLKTSGSPFTVTPQKHFNGRRYRVDFLVTIEGMTRNGEP
                     KSYVIEFDESFHAEKRQREMDEERDRWFRKHEKTLTLIRVKHEHQDDWLKCLEGIGEPR
                     SIEHYYAHCIRVASKIKSTTERVITGESSKAAYDPAKNGCSCLLKREKQRLKELSDIMT
                     TLNIPFTATDRLFVTRRSPSKYGL"
                     /name="hypothetical protein"
     gene            3294..3515
                     /locus_tag="LCD48_RS26760"
                     /old_locus_tag="LCD48_26770"
     CDS             3294..3515
                     /locus_tag="LCD48_RS26760"
                     /old_locus_tag="LCD48_26770"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063617153.1"
                     /translation="MSISNDILQDIQSGALEPFCRDGLFPREQTFILMFWDQYVAGGNA
                     GWEDYRPALDELVVSGHLKHSPNGWYVF"
                     /name="hypothetical protein"
ORIGIN
        1 aggattcttg tcagtatgat tccgcgattt gcccggatac atctacacgt tttttccagc
       61 acatctaaca cttgctgtgc tctggtgtaa ttctcgtaca tttgacctcc tgctatctgg
      121 ttttagttgt ctctatggat tgatattagc cgtatttgct ggagatatag cggtgtgaca
      181 tagtgtgatc atgtcactta cgatcacact attaattcag gtggtgtcac atggctagtc
      241 acagtgtcac tgaggctgca aagttagcgg gtgtcacgcg acggacaatc tatcggcaca
      301 ttaaggctgg caagctttct gctagtgtga caagcagtga caatacggtt attgaaacca
      361 gcgaattact ccgtgtttat ggcgtgttgt cacagcctga acctgagatg gtatccactg
      421 gatcacacga aaatcaacct gaatatgtca cacttttatt ggctgaaatg tcacaacttc
      481 gggagcagat tagtacgttg gcgagtaagg tcgatgagct tcaagaacgg cttgctctac
      541 catcacctac taagacggat cagagtgtct tggattgggt tagtgagcgg cgtaggttaa
      601 ataagaaaaa tagagccaaa taagccgatg tgggtacaag caacatgaac gcttgcggat
      661 cgtcttttgg tctttggcgt ttagtctggg cttctgtcga gtaccgttct tttatggcac
      721 ggtgaacacc acggtagagc ttgcctattt cgctgacaag ttacgttccc acgtcttgct
      781 cgttttagtc gccccgcttg aagggctgcc actggtcagt gtgtggttat tgtccgttgg
      841 ttagtttttc tgttggacgt ggacactacg ccccaaccct tgctagaatt atcctcgaga
      901 gacccgccaa gatttctcaa ggtaattttt agtagcagcg gcactgtgat aaagagaatc
      961 agattagcta ctggttttct agcaaggcac tgaccaatta agattaccac aagcccttga
     1021 ttgctgagaa gtgatcaagg gctttgttct attaggtact atgtccatct aatttctcct
     1081 cagcattcag tgcattcctt accgttccca ctgcacagcc cagcatctta gctacctttc
     1141 tctgcgagtt tccctgagca atcagcgtcc gaatatgctc atgtttttct aggtcaggtt
     1201 gtcggccttt gtagagtccc atttcttttg ccttctcaat gccctgagtt tgacggcggc
     1261 ggcggtcggt gtagtctttg cgggctatgg ctgcgagcat gtccaacatc atcccattga
     1321 tggcattcat catcgaacgc atgaactcat cgttcccgga ttgcagttgc aggtagctgg
     1381 ttggcagatc tagagcgact atccggattc ccttgctctg aatgacacca cggaggcgtt
     1441 cccaatcatc agcatcgagg cgactcaggc ggtctatttg ctcaaccagc agaatgtcac
     1501 cagcctgagc tactgccaga agccggaaca gttctggacg ctgtagcgat gccccagaga
     1561 cgttctcgat gaagtttgca gcgatggtta gcccgtgttc ttccgcaaag gtgtgcagtg
     1621 attgtgatgc acgggcagcg tcttgttcgg cggtggatgc ccggaggtag gcgtacgttc
     1681 tcatgtaatc ctctgtgtca gtttggtgta tcactatggt agtggtgcat tttgagttat
     1741 cggcggggtc taaatgtacc ggtttttagg gggggcaagc cgctatagct gaggtatacc
     1801 cataatgaac gggttagata gtgccggaca gcccgtcgat gatggctgca aggaatgccg
     1861 tcgcagctgc tatgagtgcc ttcctgaata acgtcatgaa tcgcattttg agagcctcta
     1921 aggtgtgcat gtgggtttgg gtgtgaattt tttactcatc gccttacagg gcgtctgacg
     1981 gcttgtgatg agtgttttat gggtgagtct cgtttggtat gaaattctga tacctgtgat
     2041 tcccggattt gttggttctg tggggctttt ggctgggtta atgccgatga gttaggccag
     2101 atttcatctc atggctggaa ttgttgattt gcttaccgtc taggtaaata cgatgaagag
     2161 aaaccaccta agaagataga aggcaagtta ataattaata aggggtaaat atctatataa
     2221 ggggcttgag gggacgtttg aaaggttaca acgtttgagc accccaaaaa ccgtagtgca
     2281 tcaacatttc gttgtaactt ttcatgcaac ttttagcgtt ataggccgta tttgctgggg
     2341 ctgcgacggg ttacgaacag tcgatcggtt gccgtgaatg ggatattcag tgttgtcatt
     2401 atgtcagaca gctcttttag acgttgtttt tctcgcttta gcaggcagga acagccattt
     2461 tttgccgggt cgtaggccgc ttttgatgat tcccctgtaa ttacccgctc cgttgttgac
     2521 ttgatcttgc tggcaacccg gatgcagtgg gcataatagt gttcgattga gcgtggttcg
     2581 ccgatgcctt caagacattt aagccagtcg tcttggtgtt cgtgcttcac acggatgagt
     2641 gtcagcgtct tttcatgctt tctgaaccat ctgtcacgtt cttcatccat ttctcgctgt
     2701 cgtttctctg catggaacga ttcatcaaac tcaatcacgt atgattttgg ctcgccgtta
     2761 cgtgtcattc cttcgatcgt cacaaggaaa tctacgcggt atctcctgcc gttgaaatgc
     2821 ttctgcgggg tgacggtgaa ggggctgcca ctggttttaa ggactccgat gagccgtctg
     2881 ccaaatacgg cttcatgctt actttttccg gacataaccg ggagttttac ccctgtttta
     2941 tcttcgattt cgttgatgag gtcatcgttc tggtagctat cagggctgtt tgatagtaag
     3001 gccagagcgt caaatgccga aatccagtct gtatggtgat cccattcccc ggaaattagc
     3061 tctggtgtgc aggtttcttg ctgttcggtt ggagccccca gaagattcgt cagtgcagct
     3121 tggaaccatt gggcgggtct tttcctctgt cgttctggga gtttgtcgat gagatatggc
     3181 aggaatactt cgtatgtccg accatctgtt cttagtgccg ctggttttag taacaagtgt
     3241 cctcctggat tttttcataa atataataac aacaacctct taaggagaat tttatgagta
     3301 taagtaatga tattttgcaa gacatacaat ctggagcgtt agagccattt tgccgtgatg
     3361 gacttttccc acgggaacaa acgtttatcc tcatgttctg ggatcagtat gttgcgggcg
     3421 gcaatgctgg atgggaggac tatcgaccag cgttagatga actggtcgtt agtggccatc
     3481 tcaaacatag ccctaacggc tggtacgtgt tttagcatct catttgctgt aattcgtagc
     3541 tcattttctt aagttgattt acgtattgcc aaggtgctgg tttccctttc aaccgttcta
     3601 gagcttgata aatccacgcc gtcaagttgc tgatgatgat tgccgctttt cgtgggtcga
     3661 tgctggggat tagcactttt acgcaccacg ttgggctgct agccaggttt gacgctacgt
     3721 ctatcagttg cgggtcaacg tcagtaaacc gccgttcgcc gatcatcatt ctgcgagcca
     3781 gctcgatgac cgttgggtgg tcttgcaggt atgccatgta gaagcctttc gttgcgggtg
     3841 cttctgttct gacaaaggct ttatcttcgg tgttcaggcg agacatatgc ccctcaatga
     3901 atgacatggc ttcgcagaac cttttggcct ctttagggcg tagtgagtcg attaaatagg
     3961 cgattgatgg ttctgtcatg agcccttttc ggttg
//
