LOCUS       NZ_CP083839             5933 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Enterobacter asburiae strain AR0468-yvys plasmid pAR0468_5, complete
            sequence.
ACCESSION   NZ_CP083839
VERSION     NZ_CP083839.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21379552
            Assembly: GCF_023023265.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Liao,W., Cui,Y., Quan,J., Zhao,D., Han,X., Shi,Q., Wang,Q.,
            Jiang,Y., Du,X., Li,X. and Yu,Y.
  TITLE     High prevalence of colistin resistance and mcr-9/10 genes in
            Enterobacter spp. in a tertiary hospital over a decade
  JOURNAL   Int J Antimicrob Agents 59 (5), 106573 (2022)
   PUBMED   35307563
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Liao,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023023265.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 03:55:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,365
            CDSs (total)                      :: 5,251
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 43 of 177
            Pseudo Genes (incomplete)         :: 127 of 177
            Pseudo Genes (internal stop)      :: 36 of 177
            Pseudo Genes (multiple problems)  :: 24 of 177
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083839.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="AR0468-yvys"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:61645"
                     /plasmid="pAR0468_5"
                     /geo_loc_name="China"
                     /lat_lon="30.15 N 120.10 E"
                     /collection_date="2018-04"
                     /collected_by="Yushan Cui"
     gene            complement(join(5471..5933,1..89))
                     /locus_tag="LCD48_RS26700"
                     /old_locus_tag="LCD48_26710"
     CDS             complement(join(5471..5933,1..89))
                     /locus_tag="LCD48_RS26700"
                     /old_locus_tag="LCD48_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000654246.1"
                     /translation="MKDMKAVVVFTGKDLNIMRTEGGSGYWHARTDRLNDADYLIAVRN
                     RRETWAVKDLEHGTAFLIAKITGCFKSPDYDDRNVITFDEYAEIHTPKAWKMLTDGQRY
                     PVAYLSAQEAFLRIGVTPEQLEWKKFHPSSPSVPNTVIPGLAEEKTEKLSLNEAIERAK
                     KDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     gene            complement(583..1137)
                     /gene="aac(6')-Ib"
                     /locus_tag="LCD48_RS26705"
                     /old_locus_tag="LCD48_26715"
     ARG             complement(587..1137)
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1319..1687)
                     /locus_tag="LCD48_RS26710"
                     /old_locus_tag="LCD48_26720"
     CDS             complement(1319..1687)
                     /locus_tag="LCD48_RS26710"
                     /old_locus_tag="LCD48_26720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059931.1"
                     /translation="MKTTSEFARDAQKWDDRELGASEDHVEVASMAEMSAFNDALSLQA
                     ISIRLQKNLVSDLKSIAESYGIGYQPMVRDLLQRFVIAEKKQALRRELDKLNEREEMHQ
                     QEDTVPVDQFIESIRKQA"
                     /name="hypothetical protein"
     gene            complement(1885..2178)
                     /locus_tag="LCD48_RS26715"
                     /old_locus_tag="LCD48_26725"
     CDS             complement(1885..2178)
                     /locus_tag="LCD48_RS26715"
                     /old_locus_tag="LCD48_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052905524.1"
                     /translation="MTIVISKEIEDKLTNKHKVTIKEVHESLANFKGNLLIDNREQHRT
                     NPPTNWFIAETNQRRELKVCFMIIDGDIHIKTAFEPNELERKIYAKHGKKTA"
                     /name="hypothetical protein"
     gene            3567..3890
                     /locus_tag="LCD48_RS26720"
                     /old_locus_tag="LCD48_26730"
     CDS             3567..3890
                     /locus_tag="LCD48_RS26720"
                     /old_locus_tag="LCD48_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4099..>4533
                     /locus_tag="LCD48_RS26725"
                     /pseudo=""
     CDS             4099..>4533
                     /locus_tag="LCD48_RS26725"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4568..5053
                     /locus_tag="LCD48_RS26730"
                     /old_locus_tag="LCD48_26740"
     CDS             4568..5053
                     /locus_tag="LCD48_RS26730"
                     /old_locus_tag="LCD48_26740"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015059929.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSTLLTASGASILWWQGQQIL
                     DNYTIIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            5057..5272
                     /locus_tag="LCD48_RS26735"
                     /old_locus_tag="LCD48_26745"
     CDS             5057..5272
                     /locus_tag="LCD48_RS26735"
                     /old_locus_tag="LCD48_26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     ARG             complement(584..1260)
                     /name="AAC(6')-Ib8"
                     /gene="AAC(6')-Ib8"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          3280..3463
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.638"
                     /coverage="86.0"
                     /evalue="2.7100000000000003e-47"
     Typing          3881..5376
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.385"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctggcatgcc agtagcctga ccccccctca gttctcatta tgttcaaatc cttcccggta
       61 aaaaccacaa cagctttcat atctttcata tagaaaccct taataggtga gtaaatttca
      121 tttttttatg aaatctatac gatagaattc gtgttgtaaa tgttttttat gcgataaaat
      181 acagccccct acctctttta ctgttagtgg taaggctttc tgtttgattt tttgataaac
      241 cggttaaaac aggggcttgg acgaggatat acattgaaag catgagccat tcacgtggct
      301 gcatggctat gcagtcgcgt gaaatttatc aggcgcagaa tgcgcggttt agcggggagt
      361 ttactgccgg ttttaccggg ctaacgccgc tttcacgctg aacccgtccg gagcggtgcg
      421 ggcaaggggt gttatggtaa atcatgccga cctgcaggta taaaaaaagc cccgaagggc
      481 tttgctgaca acttactttc ctgaagattt actgggtttc aacgtttgac atgaggggcg
      541 gccaagggcg ccagcccttg gacgtccccc tcgatggaag ggttaggcat cactgcgtgt
      601 tcgctcgaat gcctggcgtg tttgaaccat gtacacggct ggaccatctg gggtggttac
      661 ggtaccttgc ctctcaaacc ccgctttctc gtagcatcgg atcgctcgca agttgctcgg
      721 cgacgggtcc gtttggatct tggtgacctc gggatcattg aacagcaact caaccagagc
      781 tcgaaccagc ttggttccca agcctttgcc cagttgtgat gcattcgcca gtgactggtc
      841 tattccgcgt actcctggat cggtttcttc ttcccaccat ccgtccccgc ttccaagagc
      901 aacgtacgac tgggcatacc caatcggctc tccattcagc attgcaatgt atggagtgac
      961 ggactcttgc gctaaaacgc ttggcaagta ctgttcctgt acgtcagcaa gtgtcgggcg
     1021 tgcttcttct ccgccccacc actcgacgat atgagatcga tttagccact catagagcat
     1081 cgcaaggtca tgctcagtca tgaggcgcag tgtgacggaa tcggtgctgt tggtcacgat
     1141 gctgtacttt gtgatgccta acttgtcagt atttccaggc gtctttttat tgtcgcgttg
     1201 gcgttgatca ggttgccccg tcgattcggc aattcttttt ggtccgggtt taaggctcat
     1261 ggtaatcctc cttttaaacg tgatgatgag attctttgta tctagatggt aagttttctt
     1321 atgcttgttt tcttatgctt tcaatgaact gatcaacagg aaccgtgtcc tcctgctgat
     1381 gcatttcttc tcgttcatta agcttgtcta actctctacg taatgcttgt tttttttctg
     1441 caataacaaa tctttgcaga agatctctaa ccattggttg ataaccgata ccatagcttt
     1501 cagctatcga ttttaaatcg cttacaagat ttttctgtaa tcttattgag atggcttgca
     1561 gtgataatgc atcgttgaaa gctgacattt cagccattga tgctacttca acgtgatcct
     1621 cagaggcgcc gagctctcga tcatcccatt tctgtgcatc acgtgcgaat tcactggtag
     1681 ttttcattgt atagacctct aggtgctgtt tcctgcagat caggattagc tgcaattttt
     1741 atgcttgatc tagttccgaa aggaacttct atgttgagaa atcaggttca gcttcatttt
     1801 agttttgttt tttttcgctg aggtaagctt tttgcaaggg cttatcatgt aagcccttgc
     1861 aaaataataa cttatttagc gctgttatgc cgtcttcttt ccatgcttag catagatttt
     1921 acgttctaat tcgtttggct cgaaagctgt tttgatatga atgtcgccat cgataatcat
     1981 gaaacacact tttaattctc tacgctggtt agtttcagcg ataaaccagt tagttggagg
     2041 attagtccgg tgctgctccc tgttatctat caacaagttg cctttaaaat tcgcaaggct
     2101 ttcatgtact tcttttatcg ttaccttgtg tttattggtt agtttatctt caatttcttt
     2161 actgattaca attgtcatat tccatgatgc cccggccttt gttgtgaaat tggatttatt
     2221 gtatatacaa aatttacact tgtcaatttt gtccagcgtt ggtagggtta ttgtctgttt
     2281 ttttgtatat acagcgactg agaggaaact gcgttttctc tgcttttggc ctattgctgt
     2341 tggttatgaa agatagatat aggagaaatt atgagcaata caatagcgtt gcctcatcca
     2401 tgtcattgtg ctgactatga ttgtcgatta aacccagtcg tggcttcgac ctatcaagaa
     2461 gttgaaactc tatttggctt tagaaattta cgcggtaacg tagcccctca gacgtggtgt
     2521 agaggctgtc gcgctaaagg cgctgcagat agcaaaagga aaaaggcaca gtcttaagtt
     2581 gaggatcttc ttgagatcat atgttagtcg tcgtaatctc ttctatccaa aacgaaaaaa
     2641 ccaccctggc aggtggtttt tcgaaggtta aatgatcctg gcagatcttt taaccgtggt
     2701 aactggcttt aacggagcgc agtaaccaaa actgtccttc cagtgtagcc gtagttatgg
     2761 tgacacttca agaactctga tttttctatt gtgtactttc ccgctccgta attcagttac
     2821 cgttggctac cgccagtggc gatttgtcgt gtccgtcagg gttggaatca agagaacagt
     2881 tacctgatcg gacgtggtag tcggactaaa cggggagttc gtgcatatag tccagcttgg
     2941 agcgaaccac ctaacaggaa cccagttgca tgggagggaa tgagaaaacg ccaccttacg
     3001 gcggtctgca acgggtaaac ctcacggtgg gaacaggaga gcgcacgagg gagcgaccca
     3061 tcggaaacgg tggggatctt taagtcctgt caggtttcgc cccttctgat ttgagcaccg
     3121 gccatcgtct gatttccgtg aagctcgtca ggagggcgaa gcctatggaa aaacggcaac
     3181 gccgcatcct ttttccgcct ttcctgactt cttgctatcc cgttatctgt aagcccgtaa
     3241 cgcccgccgc agtctcacgg caggagcgta gcgaccgagt gagcgaggag gcgtcatgtc
     3301 accgacaccg gcatttcgca cctactccct gcagcagcat tttccccctc tcacatgaag
     3361 cactttcctg aatctccatc tcgccgtaac atactaagcc agtatacact ccgctagcgc
     3421 taggtgactg gttcagggct gcgccccgaa acctgctaaa accactgacg cgcctgcggc
     3481 ttgtccaaca ccgcgccgac cgggaaaaga tttcccgccc gtcccggcgt tatcaggagg
     3541 ctggattggc agacaaacgc agtaaaatgc tcacgatgtg ggtgaccgaa gatgagcacc
     3601 gccgtctgtt ggaacgctgc gacggtaaac agcttgcagc ctggatgcgc cagacgtgct
     3661 tggacgagaa acccgcccgc gccggcaagc ttccctcgat ctcgccggcg ctgcttcgtc
     3721 agcttgccgg catggggaac aacctcaacc agattgcccg gcaggttaac gccggtggtg
     3781 gcagcgggca cgaccgcgtg caggtcgtcg ccgcgctgat ggccatagat gccggactcg
     3841 agcggctgcg gcatgccgtg ctggaaaagg gtgcagacga tgatcgttaa gtttcacccg
     3901 cgagggcgcg gcggtggcgc cggtccggtg gattatctgc tggggaaaga ccgacagcgc
     3961 gacggcgcca ccgttttgca ggggaagccg gaagaagtca gggagcttat cgacgcctcg
     4021 ccctacgcaa aaaagtacac atccggcgtc ctgtcctttg ccgaaaagga tttaccgccc
     4081 gggcagcgcg aaaagctgat ggcgagcttt gaacgggttc tgatgcccgg actcgataaa
     4141 gaccagtaca gcgtgctgtg ggttgagcac caggataagg ggcggctgga gctgaacttc
     4201 ctgatcccga acatggagct gctgactggc aggcgtctcc agccgtatta cgaccgcgcc
     4261 gaccgtccgc gcattgatgc ctggcagacc atcgtgaacg gcaggctggg gctgcatgac
     4321 ccgaacgcgc ctgaaaaccg gcgggcgctg gtcacgccgt ccgcgctgcc gaaaacgaag
     4381 caggaagccg ctcaggcaat tacacggggt ttactggcct tagcctcgtc cggggagctg
     4441 aaaacccgcc aggacgtcac tgaggcgctg gaaagcgcag gttttgaggt ggtacgcacc
     4501 acaaaaagca gcatcagcat tgccgacccg gacggggggc gaaacatccg actgaaggga
     4561 gccatctatg aacagtcttt taacgctggc aaaggactta gagcagaaat cgaaagcgca
     4621 gcagcagagt accggcgaga tgctgaaagc cgcattcagc gagcacgaga agtctgtcag
     4681 agcggaactg agcgaaagcg agaagagaat cagcgccgcc atcctcgacc acgaccggaa
     4741 gctgtcctca gccatgagcc agcgcacgaa aggcatggtg cgtatggtca gccagacgtg
     4801 gctgaccatc gtcctggtgt ctacactgct gacagcatcg ggcgcaagca ttctatggtg
     4861 gcaggggcag cagatactcg acaattacac gatcatccgg gagcagaaga gcacgcaggc
     4921 catgctgtcc gagaggaaca gcggcgtgca gctctcgacc tgcggagagc agagacgccg
     4981 ctgcgtgagg gtgaacccgg aagcgggacg gttcggagag gattcgagct ggatgatact
     5041 ggcggggaaa tagcacatga cggagctgga aaaacagttg ctgagcgcat tagagcagct
     5101 acagcaggac tactcgaaaa ggctggacga gtgggagaac gccttcgcgg aatggcggac
     5161 gatgtctggt cttatgcaac gggagaacgc ggcgctgagc gagcgcgtca cgggcttgag
     5221 caggcaggtg cagagtttga gcgagcagct gcgccggttg tcacaaggct gaacgtgata
     5281 gaggcacatc gtgagcagga acgggcggcg cagcatcaga aagcgctgga gctggaacga
     5341 tctcagcgac agcgaacgta caacggtccg tcactttgaa tctgtgcgcc ggaaaagcat
     5401 gggcttgctg tttctggcgt acaggttcag ttctagaaca tgaggctgct atcttgttgt
     5461 tcacgcttca tcagatcttg atgcttattg ttatggctga gctatcaatc cctgttgcgt
     5521 tgctaatatc ctttttggct ctttcaattg cctcgttaag ggagagtttt tctgttttct
     5581 cttcagccag accaggaatc actgtattag gaaccgatgg ggatgaagga tggaatttct
     5641 tccattccag ttgttccggt gttaccccaa tacgtaaaaa agcttcctgt gcgctcaggt
     5701 aagcaaccgg atagcgctga ccatcggtta gcattttcca tgcttttggc gtgtgaattt
     5761 ctgcgtattc atcaaaggtt atgacgttcc tgtcgtcgta atcagggctt ttaaaacacc
     5821 cggtaatttt ggcgatcagg aatgctgtgc cgtgttctag gtcttttact gcccacgttt
     5881 cccttctgtt tcttactgct atgaggtaat cggcatcgtt gagcctgtct gtt
//
