LOCUS       NZ_CP083838            11675 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Enterobacter asburiae strain AR0468-yvys plasmid pAR0468_4, complete
            sequence.
ACCESSION   NZ_CP083838
VERSION     NZ_CP083838.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21379552
            Assembly: GCF_023023265.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Liao,W., Cui,Y., Quan,J., Zhao,D., Han,X., Shi,Q., Wang,Q.,
            Jiang,Y., Du,X., Li,X. and Yu,Y.
  TITLE     High prevalence of colistin resistance and mcr-9/10 genes in
            Enterobacter spp. in a tertiary hospital over a decade
  JOURNAL   Int J Antimicrob Agents 59 (5), 106573 (2022)
   PUBMED   35307563
REFERENCE   2  (bases 1 to 11675)
  AUTHORS   Liao,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023023265.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 03:55:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,365
            CDSs (total)                      :: 5,251
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 43 of 177
            Pseudo Genes (incomplete)         :: 127 of 177
            Pseudo Genes (internal stop)      :: 36 of 177
            Pseudo Genes (multiple problems)  :: 24 of 177
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083838.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11675
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="AR0468-yvys"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:61645"
                     /plasmid="pAR0468_4"
                     /geo_loc_name="China"
                     /lat_lon="30.15 N 120.10 E"
                     /collection_date="2018-04"
                     /collected_by="Yushan Cui"
     gene            1181..1948
                     /locus_tag="LCD48_RS26645"
                     /old_locus_tag="LCD48_26655"
     CDS             1181..1948
                     /locus_tag="LCD48_RS26645"
                     /old_locus_tag="LCD48_26655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227850.1"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="WP_015583927.1"
                     /translation="MKYNIGYLFDLLVVITNKDLKVRYKSSVFGYLWSIANPLLFAMIY
                     YFIFKLVMRVQIPNYTLFLITGLFPWQWFASSATNSLFSFIANAQIIKKTVFPRSVIPL
                     SNVMMEGLHFLCTIPVIIAFLFVYGMRPSLSWLWGVPIIALGQVIFTFGISIIFSTLNL
                     FFRDLERFVSLGIMLMFYCTPILYASDMIPEKFSWIITYNPLASMILSWRQLFMDGVLN
                     YEYISILYITGLVLTIVGLSIFNKLKYRFAEIL"
                     /name="ABC transporter permease"
     gene            1948..2712
                     /gene="rfbB"
                     /locus_tag="LCD48_RS26650"
                     /old_locus_tag="LCD48_26660"
     CDS             1948..2712
                     /gene="rfbB"
                     /locus_tag="LCD48_RS26650"
                     /old_locus_tag="LCD48_26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen export ABC transporter ATP-binding
                     protein RfbB"
                     /protein_id="WP_246917710.1"
                     /translation="MEPVINFSHVTKEYPLYHHIGSGIKDLVFHPKRAFQLLKGRKYLA
                     IEDITFTVGKGEAVALIGRNGAGKSTSLGLVAGVIKPTKGTVTTQGRVASMLELGGGFH
                     PELTGRENIYLNATLLGLRRKEVQQRMDRIIEFSELGDFIDEPIRVYSSGMLAKLGFSV
                     ISQVEPDILIIDEVLAVGDISFQAKCIQTIRDFKNKGVTILFVSHNMSDVEKICDRVVW
                     IENHKLREIGSAERIIELYKQASKQASKQAMA"
                     /name="rfbB"
     gene            2727..4619
                     /locus_tag="LCD48_RS26655"
                     /old_locus_tag="LCD48_26665"
     CDS             2727..4619
                     /locus_tag="LCD48_RS26655"
                     /old_locus_tag="LCD48_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004852436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="WP_246917712.1"
                     /translation="MISIKIYTCHHKPSAFLNASIIKPLHVGKANTYNDIGCEGDDSGD
                     NISFKNPFYCELTAHYWVWKNESLADYVGFMHYRRHLNFAEQQNHPEDNWGVVNYPLIN
                     AEYESQFGLSDESISTCVDGYDLLLPKKWSVTSAGSKNNLDHYAKGEFLHIKDYQSALD
                     VVEELYPQYKAAIQQFNNATDGYYTNMFVMRKDMFLDYSEWLFAILSNLEDRISMNNYN
                     AQEKRVIGHIAERLFNIYIIKCQQDKQLKIKELQRTFVTAETFNGKLKPVFDESVPVVI
                     SFDNNYALSGGALINSIVLHSDANRNYDIVVLENKVSHLNKQRLIKLVAGHNNISLRFF
                     DVNSFTEMSDVHTRAHFSASTYARLFIPQLFREYKKVVFIDSDTVVKADLATLLDVEIG
                     TNLVAAVKDIVMEGFVKFGTMSESDDGIMPAEQYLKKTLGMTNPDEYFQAGIIVFNVEQ
                     MVTENTFAQLMSALKAKKYWFLDQDIMNKVFFGRVKFLPLEWNVYHGNGNTDDFFPNLK
                     FSTYMRFLQARRNPKMIHYAGENKPWNTEKVDFYDDFLENVLSTPWEKEIYYRQLPVAT
                     VVPNQHTELQQTVLLQTKIKRALMPYVNKYAPVGSPRRNKLIKYYYKVRRSILG"
                     /name="DUF4422 domain-containing protein"
     gene            4638..5792
                     /gene="glf"
                     /locus_tag="LCD48_RS26660"
                     /old_locus_tag="LCD48_26670"
     CDS             4638..5792
                     /gene="glf"
                     /locus_tag="LCD48_RS26660"
                     /old_locus_tag="LCD48_26670"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023284018.1"
                     /GO_function="GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="WP_016247610.1"
                     /translation="MKRNNILIVGAGFSGVVIARQLAEQGHKVKIIDQRDHIGGNSYDT
                     RDPQTDVMVHVYGPHIFHTDNETVWNYVNQYAEMMPYVNRVKATVNGQVFSLPINLHTI
                     NQFFAKTCSPDEARALISEKGDSSILEPKTFEEQALRFIGKELYEAFFKGYTIKQWGMQ
                     PSELPASILKRLPVRFNYDDNYFNHKFQGMPKLGYTHMIEAIADHENITLQLQREFAAE
                     NRESYDHVFYSGPLDAFYSYQHGRLGYRTLDFERFTWQGDYQGCAVMNYCSVDVPYTRI
                     TEHKYFSPWENHEGSVCYKEYSRACGENDIPYYPIRQMGEMALLDKYLSLAEGEKNITF
                     VGRLGTYRYLDMDVTIAEALKTADKYLSSLSNDETMPVFVADVR"
                     /name="glf"
     gene            5789..6679
                     /locus_tag="LCD48_RS26665"
                     /old_locus_tag="LCD48_26675"
     CDS             5789..6679
                     /locus_tag="LCD48_RS26665"
                     /old_locus_tag="LCD48_26675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014907239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="WP_000683047.1"
                     /translation="MKHTALIVTFNRLEKLKKTVAETVKLHFTSIVIVNNGSTDGTSDW
                     LQTITDPRVIVLNLASNNGGAGGFKAGSQYICSSVDSDWVFFYDDDAYPQSDILDKFAT
                     INKEDCRVFTALVKDLQGRTCAMNVPFVKVPTSFSDTLQYIKHPQRYVPDNKKMLVQTV
                     SFVGMIIKREVLNEHLHHIYDELFLYFDDLYFGYQLTLSGEKITYQPELVFTHDVSIQG
                     KVISPEWKVYYLCRNLILAKRIFTEVEIFSSSSILLRLCKYITIFPVQRRKWVYLKFLC
                     RGIVHGVKGISGKFH"
                     /name="glycosyltransferase"
     gene            6680..7825
                     /locus_tag="LCD48_RS26670"
                     /old_locus_tag="LCD48_26680"
     CDS             6680..7825
                     /locus_tag="LCD48_RS26670"
                     /old_locus_tag="LCD48_26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_023157139.1"
                     /translation="MDIAMKKLCYFINSDWYFDLHWTDRAIAARDAGYEIHIISHFVDD
                     KIAEKFRTLGFVCHNIPLVAQSFNVLIFFRAFSKARKIIQNINPDLLHCITIKPCLIGG
                     FLAKSTHRPVILSFVGLGRVFSAESACLKLLRSFTVMAYKYIASNKCSLFMFEHDKDRA
                     KLADLVGIDYKQTIVIDGAGINPEIYKYSLEQQRDVPVVLFASRMLWSKGLGDLIEAKK
                     ILSNKNIHFTLNVAGILVENDKDAIPLATIQKWQSEGVINWLGHCSNVFDLIEESNIVA
                     LPSVYAEGVPRILLEASSVGRACIAYDVGGCDSLIINNDNGLIVKSKSVEELAEKLGFL
                     LDNPETRVAMGINGRKRIQDKFSSVMIINKTLKTYRDVVEE"
                     /name="glycosyltransferase family 4 protein"
     gene            7947..9188
                     /locus_tag="LCD48_RS26675"
                     /old_locus_tag="LCD48_26685"
     CDS             7947..9188
                     /locus_tag="LCD48_RS26675"
                     /old_locus_tag="LCD48_26685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227844.1"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_016247608.1"
                     /translation="MSEKESIAVSVIIPVHNAAGYISDTLSTVLSQTLNDIEIIIVNDC
                     SDDNTLEIVSALAETDSRIRVINNTTNLGGGGSRNIGLDAATGEYVIFLDDDDYVDNMM
                     LERMYARASDIQADVVICRSQSFDPALQVYAPMPWSVRQELLPDLKFFSSQDIPSDFFR
                     TFVWWPWDKLLRRQFITSHQLRFQEIRTTNDLFFVCAFMLMANRISVLNETLISHTINR
                     SESLSATRAESHRCAVEALVALKAFICQQGMMEHRLRDYKNYVVVFLEWHLNTISGPAF
                     HPFYQQVKEFVVALDAKSDDFYDEFIAAAHHRITTLSAEEYLFSLKDRVLKELEFFQAR
                     SSALQQEVETLTHSFAGQKDENAILHNQLHEIEERVTEQEQNIRQLTDKNNDMHHEMTI
                     KQQEFNEFISITKI"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(9229..9926)
                     /locus_tag="LCD48_RS26680"
                     /old_locus_tag="LCD48_26690"
     CDS             complement(join(9229..9677,9677..9926))
                     /locus_tag="LCD48_RS26680"
                     /old_locus_tag="LCD48_26690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223216367.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(<9986..10201)
                     /locus_tag="LCD48_RS26685"
                     /old_locus_tag="LCD48_26695"
                     /pseudo=""
     CDS             complement(<9986..10201)
                     /locus_tag="LCD48_RS26685"
                     /old_locus_tag="LCD48_26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            10295..10672
                     /locus_tag="LCD48_RS26690"
                     /old_locus_tag="LCD48_26700"
     CDS             10295..10672
                     /locus_tag="LCD48_RS26690"
                     /old_locus_tag="LCD48_26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198893.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="WP_004198893.1"
                     /translation="MPSSGPLWQLMKYGLVGIVNTLITAVVIFLLMHLGLGIYLSNAMG
                     YVVGIVFSFIANTIFTFTQPISINRLIKFLCVCFICYVANIIVIKIFFVFMPEKIYSAQ
                     ILGMFTYTITGFILNKFWAMK"
                     /name="GtrA family protein"
     gene            10669..11649
                     /locus_tag="LCD48_RS26695"
                     /old_locus_tag="LCD48_26705"
     CDS             10669..11649
                     /locus_tag="LCD48_RS26695"
                     /old_locus_tag="LCD48_26705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801547.1"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_004198905.1"
                     /translation="MTTSTDIKSTPSLAIVVPCYNEQEAFPFCLEKLSNVLNSLIARNK
                     INNNSYLLFVDDGSRDNTWAQIKDASTAYHYVRGIKLSRNKGHQIALMAGLRSVDTDVT
                     ISIDADLQDDVNCIEKMIDAYSQGYDIVYGVRCNRDSDTFFKRTTANAFYAIMSHMGVN
                     QTPNHADYRLLSNRALEALKQYKEQNIYLRGLVPLVGYPSIEVQYSREERIAGESKYPI
                     KKMLALALEGITSLSVTPLRIIAMTGFITCIISTIAAIYALIQKTTGTTVEGWTSVMIA
                     IFFLGGVQMLSLGIIGEYVGKIYIETKNRPKYFIDESVGNDSNGK"
                     /name="glycosyltransferase family 2 protein"
ORIGIN
        1 ttagctgaat aattgttact gctgttcttt gatagttacc gtactctgtt cgcctcgcaa
       61 gcaaagcagc ggaaaggctg agtctggtca atattgtttc tttttatata ttttccgaac
      121 aattcctcaa taattggtca gtattactat tgtaatgata gtaatgaaat ctattttttc
      181 ttttataatc atattgttaa gtgggtacgc tgaagtgttc gaataatgtg cagcaactgt
      241 gtattttttg gctggatgga ttggtcatgc tttttcattt ttattcttcc agaccagagc
      301 tataatgcgc gcgtaattca cgtcctacgg atgcattatt gataatgcgc agcaaacgta
      361 ccctacgacg ctcggatttg accacttttg aagaaaagtc aaattcactt ttatgcaaga
      421 taggatgagg aagtacgtta tcctggtggt gttgccgctg cctgcccgcg aacttaatac
      481 tctgcctgac tcatgtagag atgcggagcg cccagctaca tggctgttga agtcatactg
      541 ccgagagtga cgctttttgt tggtttcaag ggtgaaatga gaaagtcctg atggatagga
      601 tgccataaac tggggaaagt agcggtgcat ttgttgcggt gtagcgatgg atgtgagtcc
      661 ggagaggggc gctgagtaca gatatccgtt tgtttttagc ttttttgccg aacgctgact
      721 aggctgtttg tggcttcatg aatgctgcgc cggtaatgct tgtggagcta acgacgagcg
      781 tatcctcttc gttacggtaa gaccgggtaa gacagacact ttagagaggg gagtccgcat
      841 tttggttgcg atagccaatg gcggtagcgt gtctgaattt tagaataaac tcattgttcg
      901 atttaacatt ttatttacaa tcttaaagga tggtttatgt gtcttccacg agaatcacat
      961 tttcattcag cggttactga ggttagcgat catctccgat tccggagtgc taatgcgcag
     1021 gaaagattgt ttacatattg cttgtgcaaa ataatgttac aattggtctt tgtttatggg
     1081 aatagctgtt cgtctctcga gcacagcaga tcttatcgat aattctgttt gatacatatt
     1141 acgtacggaa ttttttgtag aataatttat gagtataaaa atgaagtata atatagggta
     1201 tttgtttgat cttcttgttg ttataacaaa caaagattta aaggtacgtt ataaaagcag
     1261 cgtatttggt tacttatggt caattgcgaa tccactgctt tttgcaatga tatattattt
     1321 catttttaag ctcgttatgc gagtgcagat cccgaattat acccttttct tgattaccgg
     1381 tttatttccg tggcaatggt ttgccagctc ggcgacaaat tccttgtttt cgtttatagc
     1441 caatgcgcaa ataatcaaaa aaacagtttt tccgcgctca gttattccat tgagtaatgt
     1501 catgatggaa gggttacatt tcttgtgtac aattcctgtg attattgcat tcctgttcgt
     1561 gtatggtatg cgtccatcgc tctcatggct ctggggcgta ccaattattg cgcttggtca
     1621 ggtgattttt acatttggta tttcaatcat attttctacg ctaaatctgt tcttcaggga
     1681 tttagagcga tttgtcagcc ttggtatcat gctcatgttt tattgcacgc cgatattata
     1741 tgcgtcagat atgattcctg aaaaatttag ctggattatt acctataatc ctctggcaag
     1801 catgatcctc agctggcggc agctgttcat ggatggcgtc ttaaattatg aatatatttc
     1861 aatcctttac attacaggtt tagtcctgac aattgtgggc ctgagtattt ttaataaatt
     1921 aaaatatcga tttgcagaga ttttgtaatg gaaccagtaa taaactttag tcacgttacg
     1981 aaagagtatc ctctttacca ccatattggc tcaggaatta aggatctcgt atttcaccct
     2041 aaaagagctt ttcagctgtt aaaagggcgt aaatatcttg caatcgaaga cattaccttc
     2101 accgtgggta aaggcgaggc cgtagcgctg attggtcgta acggcgcagg gaaaagtacc
     2161 tccctcgggc tggtggccgg cgtcattaag cctacaaaag gcactgttac aacccagggg
     2221 cgcgttgcct caatgcttga gctaggcggc ggttttcacc ctgaattaac cgggcgagaa
     2281 aatatttatc tcaatgcgac cctgcttggg ctacgccgca aagaggttca gcaacggatg
     2341 gatcggataa ttgagttctc cgagctgggt gatttcattg acgaacctat tcgcgtgtac
     2401 tcaagtggta tgcttgcgaa gcttggattc tctgtcatca gccaggtaga acccgatata
     2461 cttattatcg atgaggtact tgctgtcggt gacatttcgt tccaggccaa atgtatacaa
     2521 acaatacgag acttcaaaaa taagggtgta acgatattat tcgttagcca caatatgagt
     2581 gatgttgaaa aaatctgtga cagagttgtt tggatcgaaa accataaact ccgcgaaatt
     2641 ggctcagcag agagaatcat tgaactttac aagcaagcaa gcaagcaagc aagcaagcaa
     2701 gcaatggctt aataattggt aataagatga ttagtatcaa aatttataca tgtcaccaca
     2761 aacccagtgc ttttctaaat gcctctatta ttaaaccact gcatgtcggg aaggctaata
     2821 cttataatga tattggctgt gaaggggatg atagcggaga caacatttcc tttaaaaacc
     2881 ccttctattg tgagctgacg gcccactact gggtgtggaa gaatgaatct ctggctgact
     2941 atgtgggatt catgcattac cgcagacact tgaactttgc agaacaacaa aatcatcctg
     3001 aggataactg gggggtagtc aactacccgc tcatcaatgc cgaatacgaa agccagtttg
     3061 gattaagtga tgaatccata agcacctgcg tcgatggata tgatctgctg ttacccaaaa
     3121 aatggtcggt aacatcggca ggaagtaaga ataaccttga tcattatgca aaaggtgagt
     3181 ttttacacat taaagactat cagtctgccc tggatgtcgt tgaagagctg tatccacaat
     3241 ataaagctgc gattcaacaa ttcaataatg caactgatgg ttattatacg aacatgtttg
     3301 ttatgcgcaa agacatgttc ctggattatt ctgaatggct ttttgctatt ttgtctaatc
     3361 ttgaagatcg gatttcgatg aataactaca acgctcaaga aaaacgagtt ataggacaca
     3421 ttgctgagcg tttattcaat atctatatca tcaaatgtca acaagataaa cagctaaaaa
     3481 taaaagaact acagcgcacg tttgttactg ctgaaacatt taatggtaaa ttaaagcccg
     3541 tttttgacga aagcgtaccg gttgttatta gttttgataa taactatgca ttaagtggcg
     3601 gggcattaat caattcaatt gttctccatt ctgatgcgaa cagaaactat gatatcgttg
     3661 ttctggaaaa taaggtcagt catttaaata aacaacgcct tatcaagcta gttgctggtc
     3721 ataacaacat atcattgcgc ttttttgatg tgaattcatt cactgagatg agtgatgttc
     3781 atacccgtgc acattttagt gcgtcgacct atgcgcgctt gttcatcccg caacttttcc
     3841 gcgagtataa aaaagttgtg tttatcgatt ctgacaccgt ggtgaaggct gatttggcga
     3901 cacttctgga tgtcgagatc ggcactaacc tggttgccgc tgttaaagac atcgtcatgg
     3961 aagggtttgt gaagtttggt accatgtcag aatcagatga tggcattatg cctgcagagc
     4021 aatacctgaa aaagacatta ggaatgacta atcctgacga atattttcag gccgggatta
     4081 ttgtttttaa tgtcgaacaa atggttacag agaatacctt tgctcaattg atgtcagcat
     4141 tgaaagccaa aaagtattgg ttcttagatc aggatatcat gaacaaagtc ttctttggcc
     4201 gagtcaaatt cttaccatta gaatggaacg tgtaccatgg taatggtaat accgatgatt
     4261 tcttcccgaa tctcaagttt tcaacctata tgcgcttttt gcaagccaga agaaatccaa
     4321 aaatgattca ctatgcgggt gaaaacaaac catggaatac tgagaaagtc gatttctatg
     4381 atgattttct tgagaatgtt ttaagtacgc catgggagaa agaaatctac tatcgccagt
     4441 tacctgtggc cacggtagta cctaaccaac atactgaact gcagcaaacc gtgttactgc
     4501 agacaaagat taaaagagct ttaatgccat atgttaacaa atatgctcct gtcggttcgc
     4561 caagaagaaa taagctcatc aaatattatt ataaagttcg ccgttcgatt cttggctaat
     4621 acaactggag atacattatg aagcgtaaca atattctcat cgtcggcgct ggtttttcag
     4681 gtgtggtcat tgcccgccag cttgctgaac aaggtcacaa ggttaaaatc atcgatcagc
     4741 gggaccacat tggaggaaac tcttatgaca ctcgcgatcc tcagactgat gtcatggttc
     4801 atgtctacgg tccgcatatt ttccatacgg ataatgaaac cgtctggaac tatgtcaatc
     4861 agtatgcgga aatgatgccc tatgtgaata gagtaaaagc tactgttaat ggtcaggttt
     4921 tttcactgcc gattaatctg catactatta atcagttctt tgctaaaacc tgttctcctg
     4981 atgaagctcg cgcgctcatt agcgaaaaag gtgatagttc cattctggaa ccgaagactt
     5041 ttgaagagca ggccttgcgc ttcattggta aagaattata cgaagcgttc ttcaaaggct
     5101 acaccataaa acagtggggg atgcagcctt ctgagcttcc ggcatctata ctcaaacgtt
     5161 tgcctgtgcg tttcaattat gacgataatt atttcaatca taaattccag ggcatgccga
     5221 agttaggcta tacccatatg attgaagcga tcgccgatca tgaaaatatc actctgcaat
     5281 tacagcgtga gtttgcggct gaaaatcgcg aaagttatga tcatgtattt tatagcgggc
     5341 cgttagacgc cttctattca taccagcatg ggcgtcttgg ctaccgtact ctggactttg
     5401 agcgatttac ctggcaaggt gattaccagg gctgcgcggt catgaactat tgctcagttg
     5461 atgtcccgta tacgcgcatt accgaacata aatatttttc cccgtgggaa aaccacgaag
     5521 gctccgtatg ttacaaagaa tatagtcgcg cctgcggtga aaatgatatt ccttactatc
     5581 caatccgcca gatgggcgag atggctctgc tggataaata tctatcactg gcggaaggtg
     5641 agaaaaatat tactttcgtc ggacgcttag ggacttatcg ctatcttgat atggatgtaa
     5701 cgattgcaga agccttaaaa acggccgata aatacctgtc ttcattgtcc aacgacgaaa
     5761 caatgcctgt atttgtggcc gatgtacgat gaaacatact gcactaatag tcactttcaa
     5821 ccgtcttgag aagctaaaaa agacggttgc tgaaactgtt aagctgcact ttacctctat
     5881 cgttattgtc aataatgggt ccacggatgg gacctctgac tggctgcaga caatcactga
     5941 tccccgagtc attgtcttaa atctggccag taataacggg ggggccggcg ggtttaaggc
     6001 cggtagtcaa tatatatgca gctctgttga tagcgattgg gtcttctttt atgacgatga
     6061 tgcttatccg caaagtgata ttcttgataa gttcgcgacg ataaataaag aagattgtcg
     6121 tgtttttacg gctttagtta aagatttaca gggccggaca tgcgcgatga atgttccatt
     6181 tgtaaaagtc ccgacctcgt ttagtgatac cttgcaatat atcaaacatc cccaacgata
     6241 tgtaccagat aataagaaaa tgctggtgca aaccgtttca tttgttggca tgatcattaa
     6301 gcgtgaagtc ttgaatgagc accttcatca tatttatgat gaacttttcc tttattttga
     6361 tgatctctat tttggttatc aattaacttt aagtggcgag aaaatcactt atcaaccgga
     6421 gcttgttttt actcatgacg tgagtattca gggaaaggtt atatcgccag agtggaaggt
     6481 ttactatctt tgtcggaact tgattttggc taagagaata ttcacggaag tggaaatttt
     6541 tagtagttca tcaatccttt tgcgcctgtg taagtatatc accatattcc ctgtgcagcg
     6601 ccggaaatgg gtatatctaa agtttttatg ccgtgggatt gtacatggtg taaaaggaat
     6661 cagcggtaag tttcactaag tggacatagc aatgaaaaaa ctgtgttatt tcataaattc
     6721 ggattggtat tttgatttgc attggaccga tcgtgcaatt gccgcccgag atgccggtta
     6781 tgagattcac attattagcc attttgtcga tgataaaata gcggaaaaat tcaggacatt
     6841 aggttttgtt tgccataaca ttccacttgt cgcccaatca ttcaacgttt tgattttctt
     6901 tcgggccttt tctaaggctc ggaaaattat tcaaaacatc aatccagatt tgttgcactg
     6961 cattaccatc aagccctgtt tgatcggcgg ttttctggct aaaagtaccc atcgtcctgt
     7021 tattctgagt tttgtcggtt tgggccgagt attttccgca gagtctgcct gtcttaagct
     7081 gctgcgaagt tttactgtta tggcatataa gtatatcgcc agtaacaaat gcagtttgtt
     7141 tatgttcgaa catgataaag acagagctaa acttgccgat ctggttggta tcgattacaa
     7201 acagactatt gttattgatg gcgcgggtat taatccagag atttacaaat actctctgga
     7261 gcagcagcgt gatgttccgg tcgtcctttt tgccagccgt atgctgtgga gtaaaggact
     7321 gggtgacctg attgaagcca aaaaaatact gagtaataaa aatattcact ttacgctgaa
     7381 tgttgccggt attttagttg agaatgataa agacgcaatt ccgctggcga cgatacagaa
     7441 gtggcaaagc gaaggcgtga ttaactggct cggtcattgc tctaatgtat ttgatttaat
     7501 tgaagaatca aatatcgttg ctttgccgtc ggtctacgcc gaaggcgtac cgcgtatctt
     7561 gctggaagct tcctctgtcg ggcgcgcttg tatcgcttat gatgttggtg gctgtgatag
     7621 cttaattatc aataacgata atgggttgat tgtaaaaagt aaatctgtcg aggaattagc
     7681 ggagaaactc ggtttcctgt tggataaccc agaaacgcgc gtcgcaatgg gtatcaatgg
     7741 cagaaaacgc attcaagata aattctcgag tgtgatgatc attaataaaa cattaaaaac
     7801 atatcgtgat gttgttgaag agtaattttt gatgaagctc agtcgctttc atcttaccca
     7861 tccttaataa actggcaacc tacaaattaa acgctaccca taaataaata taattgcgtt
     7921 tataagctaa gcgtcaaggt gattgcatgt ctgaaaaaga gagtatagct gtttccgtta
     7981 ttatccctgt gcacaatgcg gcgggatata tttccgacac attgagcact gttttgtcgc
     8041 aaacattaaa tgatattgaa atcattattg tcaacgattg ttctgacgat aataccttag
     8101 agattgtttc ggcgctggct gaaactgatt cacgaattag agtcattaac aacacgacaa
     8161 atcttggtgg gggaggctca agaaacatcg ggttggatgc tgctaccggt gagtacgtta
     8221 tttttcttga tgacgacgat tatgtcgata acatgatgtt ggagcgcatg tacgcacgtg
     8281 ctagcgatat acaagcggat gtcgttatct gtcgcagcca gtcttttgat cccgccttgc
     8341 aggtttatgc tccgatgccg tggtcagtgc gccaggagct gttacctgat ctcaagtttt
     8401 tttcatccca ggatattccc tctgactttt tccgcacctt tgtctggtgg ccatgggaca
     8461 agcttctcag gcgtcaattt attactagtc atcagcttcg ctttcaggaa atcaggacga
     8521 ccaacgatct tttcttcgtc tgcgcattta tgctcatggc gaacagaatt tcggtcttaa
     8581 atgaaacgtt aatttctcat accatcaacc gtagtgaatc cctatccgcc acgcgagcag
     8641 agtcgcaccg ctgtgctgtt gaagcattgg tggcgcttaa agcgtttatc tgtcagcagg
     8701 ggatgatgga acaccgtctc agagattata aaaactatgt tgtcgtgttc cttgagtggc
     8761 atttaaacac gatttctggt ccggcatttc acccgtttta tcagcaagtg aaagagtttg
     8821 ttgttgccct ggatgcaaag agtgatgatt tttatgatga atttatcgcc gccgcccatc
     8881 ataggatcac cacgctgtca gctgaagagt acctattctc tcttaaagat cgggttctga
     8941 aggaacttga gtttttccag gccaggagct ccgcattaca gcaagaagtt gagacgctta
     9001 cccactcatt cgccgggcaa aaggatgaaa atgcgatatt gcataatcaa ttgcatgaga
     9061 ttgaagagcg ggtaactgaa caggaacaga atattcgtca gttaaccgac aaaaataatg
     9121 atatgcatca tgagatgact atcaaacagc aagagttcaa tgaatttatc agcatcacaa
     9181 aaatttaatc tcttctttat cctggaagtt gacggtaatg actccaactt actgatagtg
     9241 ttttatgttc agataatgcc cgatgacttt gtcatgcagc tccaccgatt ttgagaacga
     9301 cagtgacttc cgtcccagcc ttgccagatg ttgtctcaga ttcaggttat gtcgctcaat
     9361 gcgctgagtg taacgcttgc tgattacgtg cagctttccc ttcaggcggg attcatacag
     9421 cggccagcca tccgtcatcc ataccacgac ctcaaaggcc gacagcaggc tcagaagacg
     9481 ctccagtgtg gccagagtgc gttcaccgaa gacgtgcgcc acaaccgtcc tccgtatcct
     9541 gtcatacgcg taaaacagcc agcgctgacg tgatttagca ccgacgtagc cccactgttc
     9601 gtccatttca gcgcagacaa tcacatcact gcccggctgt atgcgcgagg ttaccgactg
     9661 cggcctgagt tttttaagtg acgtaaaatc gtgttgaggc caacgcccat aatgcgggcg
     9721 gttgcccggc atccaacacc attcatggcc atatcaatga ttttctggtg tgtaccgggt
     9781 tgagaagcgg tgtaagtgaa ctgcagttgc catgttttac ggcagtgaga gcagagatag
     9841 cgctgatgtc cggcagtact tttaccgtta cgcaccacgc cttcagtagc tgagcaggag
     9901 ggacaactga tggagatgga agccacggga gcacctcaaa aacaccatca tacactaaat
     9961 cagtaagttg gcagcatcac ccagtcgtga tggcaaggtc agaatagcgc tgaggtctgc
    10021 ctcgtgaaga aggtgttgct gactcatacc aggcctgaat agcttcatca tccagccaga
    10081 aagttatgga gccacggttg atgagggctt tattgtaggt gggccagttg gtgattttga
    10141 acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca
    10201 actcagcaaa agttcgattt attcaacaaa gccatgtagt ttgttagaat gctgccgcct
    10261 ttaacatcca attggaacgc tagcggagta tataatgcca agttcaggcc cattatggca
    10321 actaatgaaa tatgggttag ttgggatagt caatacacta attacggcag ttgtaatttt
    10381 cctgctaatg catttgggtc ttggcattta tctgtccaat gcgatgggtt atgttgtagg
    10441 tattgttttc agcttcatag caaacacaat atttacattt acgcaaccaa ttagcatcaa
    10501 tagactaata aaatttttat gtgtttgctt catttgttat gtggcaaata tcattgtcat
    10561 aaaaatattt ttcgttttta tgccagaaaa aatatattca gcacaaatcc ttgggatgtt
    10621 cacatacact atcacaggtt ttattttaaa caagttctgg gcgatgaaat gacaacctca
    10681 actgatataa aaagcactcc ttctttagct attgtggtac cttgctataa tgaacaagag
    10741 gcttttcctt tctgtctcga aaagctttcg aatgtactaa attcattgat agccagaaat
    10801 aaaattaaca acaatagtta tcttttgttt gtcgatgatg gtagtcgtga caatacttgg
    10861 gcacaaatta aagatgcctc gaccgcttat cactatgtgc gaggaataaa attatcaaga
    10921 aataaaggac atcaaattgc cttaatggca gggttacgct cggtcgatac agacgtaacc
    10981 attagcatcg atgcggatct acaagatgat gtaaattgca tcgaaaaaat gattgacgct
    11041 tacagccagg gatatgacat agtatacggc gtaagatgta atcgagacag tgacactttt
    11101 tttaaacgta caacagctaa tgcattttac gcaataatgt cccacatggg tgtaaatcaa
    11161 actccaaatc atgcagatta tcgattatta agtaatcgtg cgttggaggc tcttaaacaa
    11221 tataaagagc aaaatatata tttacgtgga ttagtgcctc ttgtgggata cccctcgatc
    11281 gaggtgcaat atagccgtga agaaagaatt gcaggtgaat caaaatatcc aataaaaaaa
    11341 atgcttgcgc tggctctcga gggaattacc tcattatcag ttacaccgtt acgaattata
    11401 gctatgacag gttttataac ttgcatcata tctaccatcg ctgcgattta tgctttaatt
    11461 caaaaaacaa caggtactac agttgaggga tggacatcag tcatgatcgc tatattcttt
    11521 cttggcggtg tgcaaatgct ttctttaggt attataggag aatatgtcgg gaaaatttat
    11581 atagagacga aaaatagacc taaatatttc attgatgaaa gcgtaggtaa tgatagcaat
    11641 ggaaaataat atgcaaaacc tgatcaattc tttag
//
