LOCUS       NZ_CP083812             4460 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Pantoea agglomerans strain 33.1 plasmid p33.1_4, complete sequence.
ACCESSION   NZ_CP083812
VERSION     NZ_CP083812.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21525196
            Assembly: GCF_020149765.1
KEYWORDS    RefSeq.
SOURCE      Pantoea agglomerans
  ORGANISM  Pantoea agglomerans
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Erwiniaceae; Pantoea; Pantoea agglomerans group.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Bonatelli,M.L., Castillo,G.A.A., Ferreira,A.P.O., Correr,F.H.,
            Tamasco,G., Leomil,L., Romao-Dumaresq,A.S. and Quecine,M.C.
  TITLE     Mechanisms of Pantoea agglomerans 33.1 to promote plant growth and
            bioremediate
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Bonatelli,M.L., Castillo,G.A.A., Ferreira,A.P.O., Correr,F.H.,
            Tamasco,G., Leomil,L., Romao-Dumaresq,A.S. and Quecine,M.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) Genetics, ESALQ/USP, Padua Dias, 11,
            Piracicaba 13418-900, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbial Assembly - SMRTLink v. 9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 153.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 02:17:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,646
            CDSs (total)                      :: 4,532
            Genes (coding)                    :: 4,440
            CDSs (with protein)               :: 4,440
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 40 of 92
            Pseudo Genes (incomplete)         :: 52 of 92
            Pseudo Genes (internal stop)      :: 27 of 92
            Pseudo Genes (multiple problems)  :: 21 of 92
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083812.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Pantoea agglomerans"
                     /mol_type="genomic DNA"
                     /strain="33.1"
                     /isolation_source="Plant tissue"
                     /host="Eucalyptus grandis"
                     /db_xref="taxon:549"
                     /plasmid="p33.1_4"
                     /geo_loc_name="Brazil:Itapetininga"
                     /lat_lon="23.321324 S 47.504833 W"
                     /collection_date="2002-03"
     gene            complement(146..469)
                     /locus_tag="LB453_RS23255"
                     /old_locus_tag="LB453_23180"
     CDS             complement(146..469)
                     /locus_tag="LB453_RS23255"
                     /old_locus_tag="LB453_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_224481807.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNGAGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1107..1238
                     /locus_tag="LB453_RS23370"
     CDS             1107..1238
                     /locus_tag="LB453_RS23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_103797730.1"
                     /translation="MHEPPVQSDCCALPGNYHLESNPERHDLSPLATAIGRGRTSDY"
                     /name="replication initiation protein"
     gene            complement(2164..2349)
                     /locus_tag="LB453_RS23260"
                     /old_locus_tag="LB453_23185"
     CDS             complement(2164..2349)
                     /locus_tag="LB453_RS23260"
                     /old_locus_tag="LB453_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017345744.1"
                     /GO_process="GO:0006950 - response to stress [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="WP_072010481.1"
                     /translation="MAGRRGGAGNFAENPERAAEAGQKGGRISGGNFKNNPERAREAGR
                     KGGQISRRGAPERKVS"
                     /name="general stress protein"
     gene            2646..2873
                     /locus_tag="LB453_RS23265"
                     /old_locus_tag="LB453_23190"
     CDS             2646..2873
                     /locus_tag="LB453_RS23265"
                     /old_locus_tag="LB453_23190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_103797729.1"
                     /translation="MYRNEEERLSDTVMGEAVIDLLDERARITNATLLQKLQTFLLATE
                     EPWREAAIRSAIRDVQSAILDVGSKASSLH"
                     /name="hypothetical protein"
     gene            complement(3224..3439)
                     /locus_tag="LB453_RS23270"
                     /old_locus_tag="LB453_23195"
     CDS             complement(3224..3439)
                     /locus_tag="LB453_RS23270"
                     /old_locus_tag="LB453_23195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3443..3928)
                     /locus_tag="LB453_RS23275"
                     /old_locus_tag="LB453_23200"
     CDS             complement(3443..3928)
                     /locus_tag="LB453_RS23275"
                     /old_locus_tag="LB453_23200"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_224481806.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDQKLSSAMRQRTKGMLRMVSQTWLTIVLVSALLIASGAAILWWQSQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILEGK"
                     /name="MbeB family mobilization protein"
     Typing          complement(579..757)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.339"
                     /coverage="83.0"
                     /evalue="7.539999999999999e-48"
     Typing          734..1215
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.525"
                     /coverage="93.0"
                     /evalue="1.2800000000000002e-163"
     Typing          complement(150..469)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.196"
                     /coverage="100.0"
                     /evalue="6.7e-63"
     Typing          complement(3121..4460)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.946"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctttttggca tagggcgagg cgtcgataag ctcccggact tcctccggct tcccctgcag
       61 aacgctggca ccgtcgcggt gccggtcttt gcccagcaga taatccaccg gaccggcgcc
      121 accgccgcgc cctcgcgggt gaaacttaac gatcatcgtc tgcccctttt tccagcacgg
      181 catgccgcag ccgctcgagt ccggcatcga tggccatcag cgcggcgaca acctgcacgc
      241 ggtcgtgccc gctgcctccc gcgccgttaa cctgccgggc aatctgattg aggttgttcc
      301 ccataccggc aagctgacga agcagcgccg gcgagatcga cggaagtttg ccggcgcggg
      361 caggcttctc gtccaggcac gtctgccgca tccaggccgc gagctgttta ccgtcgcagc
      421 gttccagcag acggcggtgc tcatcttccg ttacccacat cgtgagcatt ttgctgcgtt
      481 tatctgccaa tccggcctcc tgataacgcc gggacgggcg ggaaatcttt tcccggtcgg
      541 cgcggtgttg gacaagccgc aggcgcgtca gtgtctttta gcgggtttcg gggcgcagcc
      601 ctgaaccagt cacgtagcgc tagcggagtg tatactggct taatatggta aatcgaagtg
      661 ggaattcagg gaagtgcttc atgtggcaag ggaaaatgcg gcggcggtgc gtaagtgcag
      721 aatgtcagga ttggtgaaat gacgcctcct cgctcactcg gtcgctacgc tcctgccgtg
      781 agactgtggc gggcgttacc ggctcacaaa ttgcggaatg agagatgacg ttaaagtaga
      841 gaaggcagga aaaggatgcg aagttgccgt ttttccatag gctccgcccc cctgacaagc
      901 atcactaaat ctgacgctca aatcagtggc ggcgaaaccc gacaggacta taaagatccc
      961 aggcgtttcc ccctggtagc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
     1021 gtgtcattcc gctgttatgg ccgcggtttc gcattccacg cctgacactc agttccgggt
     1081 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gactgttgcg
     1141 ccttgcccgg taactatcat cttgagtcca atccggaaag acacgactta tcgccactgg
     1201 caacagccat tggtagaggg cgtacaagtg attactgatg cagagttctt gaagttagag
     1261 ccaaactacg gctaaactga aaggacgaat ttggtggcag cggtcttgta caccctgtta
     1321 ccacggttca gaagttcccc aacttactga accttcgaaa aaccacctcc ccaggtggtt
     1381 ttttcgttta gtcagcaaga tactcgccaa gcctttctct cgtcaatccg cttcaacatc
     1441 aatcatccaa taaatcatca cacgtggtta agaaaatcac tgttggtgtt taaaatagca
     1501 ctaaaagctt aaggctcatt tgaatactgt tgtattattt tcgtattatg ggcgattttt
     1561 tgttgcgtgt tttttcgtga tgctgtttaa tgcttctgac gcatagcgta tccgttgcct
     1621 ttttgatttt aatggctaat atcggatttg gttaaccttg gggacaccta gggctagact
     1681 gggcgcaacg ttatgcgggg gtttcgaaaa ctcctggaag cgaaaagcca ccggatagat
     1741 agaaaaaacc ctcgcaggac gttgcattac ttattggcaa ctagcgaggg ctcattcgtg
     1801 atttggcgat ctaagaatag ccgcaagcgg ttgccgttgc aagcgcgagg gcttttctat
     1861 gatgttcgta aatgcttgca agtggattgg caatatcatc ctccaaatcg caattggcgt
     1921 agccggtagc gttttggcaa gtgtgattct tgcccatttc catcttgcgt aaagggcacg
     1981 agcggatgtc atagttctcc gctttgcagc ggtaacacgt tgcgcccgaa ctggccgctg
     2041 gggataccta gcggcctttt cgcaactcta atttaactcc tcaaacgctg ttgcttttac
     2101 cataacatcc cttttgcgca gggcatgaaa aaacccgcct gggcgggttt tgggcagctc
     2161 atctcaggaa accttgcgct caggagcacc tcttcgactg atctggcctc cctttctgcc
     2221 tgcttcacgg gctcgctctg ggttgttctt gaagttccct ccgcttatgc ggccaccttt
     2281 ttggccggct tctgcagccc gttctggatt ttccgcaaaa ttgcctgcac cgcctctacg
     2341 tcccgccata acgcctccgg tttgagttaa gtaaagacag ataaagcata gccttaacct
     2401 tttctctctg cgcctgatca ggcgtgcgca gcgttcagac tgcctcaccg atcgtttttt
     2461 gacgttttta aggcaaaaaa aaacccgctc agggcgggga aaattcgtta cagaggaata
     2521 tctctgtctc taatagggca acgtaaaact atttaagttc cttccctgcc agggcggttg
     2581 ttttcacgta ttctgtaggc ttcgcctgcc gtttaggcag agctttcaca gcaagcaggt
     2641 cacgcatgta cagaaacgaa gaagaaaggc tgtctgacac agtaatgggc gaggccgtca
     2701 ttgatctgct cgatgagagg gcgcgcataa ccaatgccac actgctccag aagctccaga
     2761 ccttcctgct cgcaacagag gaaccgtggc gggaggcagc gatacgcagc gctatcagag
     2821 acgtccagtc ggcaattctg gatgttggca gcaaggcgtc atctctgcac tgagcgcggc
     2881 aactccgggt gaacaggcca ggcacccgga tttaacataa gagttcttag cggcaccgcc
     2941 ccggacgggt tcggcgcgaa agcggcgtca gcccggtaaa accggcagta aactccccgg
     3001 taaaccgcgc cgcctgcacc agataaattt aacccaaatg catagccatg cagccgggtg
     3061 aatggctccg gctttcggcg gggatctgcg ccgaaatccc cgttttatcg ggtttgtcac
     3121 agcgacggac cgtcatacgt ccgctggcgc tgcgatcgtt ccagttccag cgctttctgg
     3181 tgctgcgccg cccgctgctg ctcccggtgc gcctcgattc cgttcatcct tgcgacaacc
     3241 ggcgcagctg atcgctcaaa ctctgcacct gcctgctcaa gcccgtgacg cgctcgctca
     3301 gcgccgcgtt ctcccgttgc ataagcccag acatcgtccg ccattccgcg aaggcgttct
     3361 cccactcgtc cagccttttc gagtagtcct gctgtagctg ctctaatgcg ctcagcaact
     3421 gtttttccag ctccgtcatg tgctatttcc cctccagtat catccagctc gaatcctctc
     3481 cgaaccgtcc cgcttccggg ttcaccctca cgcagcggcg tctctgctcg ccgcaggtcg
     3541 agagctgcac gccgctgttc cgctcggaca gcatagcctg cgtgctcttc tgctcccgga
     3601 tggtcgtgta attgtcgagt atctgctgac tctgccacca cagaatggcc gcgcccgagg
     3661 ctatcagcag cgcggagacc aggacgatgg tcagccatgt ctggctgacc atccgcagca
     3721 tccctttcgt gcgctggcgc atggctgagg acagcttctg gtcgtggtcg aggatggcgg
     3781 cgctgattct cttctcgctt tcgctcagtt ccgctctgac agacttctcg tgctcgctga
     3841 atgcggcttt cagcatctcg ccggtactct gctgctgcgc tttcgatttc tgctctaagt
     3901 ccttcgccag cgttaaaaga ctgttcatag atggctccct tcagtcggat gtttcgcccc
     3961 ccgtccgggt cggcaatgct gatgctgctt tttgtggtcc gcaccacctc aaaacctgcg
     4021 ctttccagcg cctcagtgac gtcctgacgg gttttcagct ccccggatga ggcaagggca
     4081 agcagaccgc gcgtaatcgt ctgggcggct tcctgcttcg tttccggcag cgcggacggc
     4141 gtgaccagcg cccgccggtt ctccggcgcg ttcgggtcgt gcagccccag cgtgccgttc
     4201 acgatggtct gccaggcatc gatgcgcgga cggtcggcgc ggtcgtaata cggctggagc
     4261 cgtctgccgg tcagcagctc cgtgttcggg atgaggaaat tcagctccag ccgtccctta
     4321 tcctggtgct caacccacag cacgctgtac tggtctttat cgagtccggg catcagaacc
     4381 cgctcgaagc tcgctatcag cttttcacgc tgaccgggcg gtaaatcctg ttcggcaaac
     4441 gacagcacac cggaggtgta
//
