LOCUS       NZ_CP083801             3514 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF019 isolate INF019 plasmid
            pINF019_5, complete sequence.
ACCESSION   NZ_CP083801
VERSION     NZ_CP083801.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3356962
            Assembly: GCF_020149785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Hawkey,J., Cottingham,H., Tokolyi,A., Wick,R., Judd,L.M.,
            Cerdeira,L., de Oliveira Garcia,D., Wyres,K.L. and Holt,K.E.
  TITLE     Linear plasmids in Klebsiella and other Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2021) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 15:43:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,723
            CDSs (total)                      :: 5,594
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 72 of 152
            Pseudo Genes (incomplete)         :: 86 of 152
            Pseudo Genes (internal stop)      :: 34 of 152
            Pseudo Genes (multiple problems)  :: 30 of 152
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083801.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF019"
                     /isolate="INF019"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pINF019_5"
                     /geo_loc_name="Australia"
                     /collection_date="2013-04"
     gene            complement(349..540)
                     /locus_tag="LB481_RS28630"
                     /old_locus_tag="LB481_28630"
     CDS             complement(349..540)
                     /locus_tag="LB481_RS28630"
                     /old_locus_tag="LB481_28630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040118553.1"
                     /translation="MLKKNEIILIKSCGNSKTKIFLRGVDEGLMIDASFEDIKFAINNN
                     LHDLLGIQAETTKLKLAK"
                     /name="hypothetical protein"
     gene            1711..2052
                     /locus_tag="LB481_RS28635"
                     /old_locus_tag="LB481_28635"
     CDS             1711..2052
                     /locus_tag="LB481_RS28635"
                     /old_locus_tag="LB481_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2261..>2689
                     /locus_tag="LB481_RS28705"
                     /pseudo=""
     CDS             2261..>2689
                     /locus_tag="LB481_RS28705"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2730..3227
                     /locus_tag="LB481_RS28645"
                     /old_locus_tag="LB481_28645"
     CDS             2730..3227
                     /locus_tag="LB481_RS28645"
                     /old_locus_tag="LB481_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            3234..3449
                     /locus_tag="LB481_RS28650"
                     /old_locus_tag="LB481_28650"
     CDS             3234..3449
                     /locus_tag="LB481_RS28650"
                     /old_locus_tag="LB481_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1454..1626
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.655"
                     /coverage="100.0"
                     /evalue="9.54e-56"
     Typing          complement(983..1529)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2043..3514
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 gtaccggaac ggcaacgcac attatctcat ggtgacaggg agtatggaga ctgataaacg
       61 cccgtctcag gtggcctgag cgtgacgggg atcatggcca ttttctgcga tttcagcaca
      121 cagagagtct tttttaaccg aaatcaccgg ctatgcagtg atgtgaataa gtgatgcata
      181 tgcgtaaaat aaatcaggtg cgtttctggt ggttttcggt gtacttgcca ccaccttttg
      241 cccgtcagcc atcagaaacg ccctgagtgc ctccggcgcg gtgcgggtaa gaagacgtta
      301 tggtaaatcg gtggctttat ttcgatcctt catgcttcag gggctgtatc actttgctag
      361 ttttagtttt gtggtttcgg cttggattcc gagaagatcg tggaggttat tgttgatagc
      421 aaattttata tcttcaaaac ttgcatcaat catgagtccc tcatccacac ctcgtaaaaa
      481 aatttttgtt tttgagttac cacatgattt tattaagata atttcatttt tttttaacat
      541 gacattgtca ttgtttgttg tcgtcagatg aatcattatc gttgtttttg tgtgtgtttg
      601 ttaaggctgc taagattatt cctaaccact gattttgtac atatcatcaa aaaaataaaa
      661 aaactaatgc cctgtggtgg gtatgtaaca aatcacgggg agcgggtcag aactacgcca
      721 aaactttgaa tgatcgggtg gcaggctaaa tgaaatcttg agattcattc agcctcgtcg
      781 taatctcttg ctctgtaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttaagt
      841 cagttgggga actgcttaac caggtaactg gcttcagcag agcgcagata ccaaatactg
      901 tccttccagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca tcacctacat
      961 taactcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgcttt ggcgtgtctt
     1021 tccgggttgg actcaagacg atagttaccg gataaggcgc agcagtcgga ctgaacgggg
     1081 ggttcgtgca tacagtccag cttggagcga actgccttcc cggaactgag tgtcaggcgt
     1141 ggaatgagat aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac
     1201 aggagagcgc acgagggagc taccaggggg aaacgcctgg gatctttata gtcctgtcgg
     1261 gtttcgccgc cactgatttg agcgtcagat tttgtgatgc ttgtcagggg ggcggagcct
     1321 atggaaaaac ggcttcgccg cggcctcatc gcttccctgt taagtttctt cctggtatct
     1381 tcctggaaat ttccgccccg cccgtaagcc ggcaccgctc gccgcagccg aacgaccgag
     1441 cgcagcgagt cagtgagcga ggaagcggaa tatcttctgt atcgcatatt ctgcttacgc
     1501 tctgctgcag catttttctt ctgccgcata aggcgctttc ctgattatcg cactcatgcc
     1561 aacatactaa gccagtatac actccgctag cgctacgtga ctggttcagg gctgcgcccc
     1621 gaaaccccac tgacgcgcct gcggcttgtc caaccccgcg ccgaccggga aaggctttcc
     1681 cgtccgtcac ggcgttatca ggaggcgatt ctggcagata aacgcaacaa gatgctcacg
     1741 atgtgggtga cggaagacga acaccggcgg ttgcttgagc ggtgcgacgg aaaacaactg
     1801 gcggcgtgga tgcgccagac ctgccttgat gagaaaccga cacgcgccgg caggcttccc
     1861 tcgatttcgc ccgcgctgct gcgtcagctt gccggcatgg gcaataacct gaaccagatc
     1921 gcccggaaag tgaacgccgg tggtgccggt catgaccggg tgcagatcgt ggcagcgctg
     1981 atggcgatcg atgccggtct ggaacggttg cggcatgccg tactggaaaa agggccggac
     2041 gatgattgtt aaattccatc cccggggtcg cggcgggggc gccggtcctg tggattatct
     2101 tctgggtaaa gaccggcagc gtgaaggtgc cagcgtcctg caggggaagc cggaagaagt
     2161 ccgggagctg attgatgcgt cgccgtatgt gaaaaaatat acctccgggg tgctgtcatt
     2221 cgcggaggcg gacctgccgc cgggtcagcg tgaaaaactg atggccagct ttgagcgggt
     2281 tctgatgccc ggactcgata aagaccagta cagcattctg tgggtggaac acgcagacaa
     2341 gggtcgtctt gagctgaatt ttctgatccc caataccgaa ttactgacgg ggaaacgcct
     2401 ccagccgtat tacgatcgtg ccgaccggcc gcgcatcgat gcgtggcaga cagtggtcaa
     2461 cggcaggctg gggctacatg atccgaacga cccggagaac cggcgggcgc tggtcacgcc
     2521 ttcaggactg ccagaaacga agcaggaagc cgcagagacg attacacggg gtttgctgat
     2581 gttagcctca tcaggagagc tgaaaacgcg tgatgacgtc acaggggcgc tcacagcggc
     2641 cggttttgag gtggtcagaa ccacaaaaaa cagtatcagc attgccgacc cggacggggg
     2701 gcgaaatatc cgactgaggg gagccattta tgagcagtct tttaacggcc tcgagggact
     2761 tcgaggagaa atcgaaagca cagcagcagc ttaccgaaga gaggctgaaa gccgcattca
     2821 gcaagcacga gaacgttgtc aaatcggaac tgaacgcaag cgcgaagaga atcaacgacg
     2881 ccatcagcgc ccacgaacag gggatgaacg cggcgatgca gtcgaaccgg ctgagcgtgc
     2941 tgcgaatggt cgggcggacg tggctgacca tcaccatggt gtcagggctg ctgttcgcca
     3001 gcctgagcgg ggtgctgtgg taccagggga accggatagc ggcgaacctg gcggaaatca
     3061 ggcagcagag agatacgctg tcgaaactgc acatgcagac gtggggcgtg acctatctgg
     3121 agaaccgcaa cggacggttt ctggtgctgc cggaggggat gaaagccgaa acgggccgga
     3181 cggtggacaa caagacgcgg aacgcggtga agctggtacg ggagtagagc agaatgacgg
     3241 aactggaaaa acaattgctg agcgcgttag agcagctaca gcaggactac tcgagaaggc
     3301 tggacgagtg ggagagcgcc ttcgaggaat ggcggaagat gtctggtctt atgcaacggg
     3361 agaacgcggc gctgagcgag cgcgtgtcgg gcttgagcag gcaggtgcag agtttgagcg
     3421 agcagctgcg ccggttatcg acaggctgaa tgagattgag caacagcgat acgggcaaac
     3481 aacggaaaag gaaccagaga agacagcaga tctg
//
