LOCUS       NZ_CP083800             4048 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF019 isolate INF019 plasmid
            pINF019_4, complete sequence.
ACCESSION   NZ_CP083800
VERSION     NZ_CP083800.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3356962
            Assembly: GCF_020149785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Hawkey,J., Cottingham,H., Tokolyi,A., Wick,R., Judd,L.M.,
            Cerdeira,L., de Oliveira Garcia,D., Wyres,K.L. and Holt,K.E.
  TITLE     Linear plasmids in Klebsiella and other Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2021) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 15:43:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,723
            CDSs (total)                      :: 5,594
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 72 of 152
            Pseudo Genes (incomplete)         :: 86 of 152
            Pseudo Genes (internal stop)      :: 34 of 152
            Pseudo Genes (multiple problems)  :: 30 of 152
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083800.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF019"
                     /isolate="INF019"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pINF019_4"
                     /geo_loc_name="Australia"
                     /collection_date="2013-04"
     gene            complement(join(3351..4048,1..721))
                     /locus_tag="LB481_RS28615"
                     /old_locus_tag="LB481_28615"
     CDS             complement(join(3351..4048,1..721))
                     /locus_tag="LB481_RS28615"
                     /old_locus_tag="LB481_28615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_137464447.1"
                     /translation="MYHKIIGGKKSKGQRNIKKSIEYLLREKNPEQQKDIKILSTTTKQ
                     DLINFENYILDKNKSNPYVCGVLSFEEKSISEELKTKLITEFEDILFAGIEQENRPPVM
                     WVEHNDKGRVELNYLTFNSLQDRRSYTVYLDKRDRGLVNNYSEIINYENNLSSPFEEKE
                     NKNTLTNKPKTNIPEDKKQFIENINTEIMALIINEELENREQTIKYIENMKGVKINRIR
                     KNQISIKSDLFDDDKPIVLKGDIYEEGRDYSDYRKEFNPKPTRDPEFVREKLTELKRTF
                     AERIEKREIRNRSKFKKPSFKNIPENRQSVQREDQDIGYKYTNGDFINVSNNDGFSGMY
                     IRTDNMEIVNNESINTEYKTKTATEETAGTTRTRDIEKINQQQQFVNSRLEEAGRKQQQ
                     FRKSIDTTEGRFGVCRRSVHSIISTFGRYIRTFSAYTRKNEHRQKFEKRRKIEEEKLKI
                     KNQGSYNRRRYKPY"
                     /name="hypothetical protein"
     gene            complement(723..1070)
                     /gene="mobC"
                     /locus_tag="LB481_RS28620"
                     /old_locus_tag="LB481_28620"
     CDS             complement(723..1070)
                     /gene="mobC"
                     /locus_tag="LB481_RS28620"
                     /old_locus_tag="LB481_28620"
                     /inference="COORDINATES: protein motif:HMM:NF017524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_048295903.1"
                     /translation="MSYKDRYNRKHSITLCFNDEEFAQVEELASMLKKPRATAVRDLIL
                     NKDFKLKKEIAKNKTGDVYFELHKIGVNINQIARAINTDIDKFISHEFSEDFLNLFEEI
                     TNEIEEIKRRI"
                     /name="mobC"
     gene            complement(1323..2735)
                     /locus_tag="LB481_RS28625"
                     /old_locus_tag="LB481_28625"
     CDS             complement(1323..2735)
                     /locus_tag="LB481_RS28625"
                     /old_locus_tag="LB481_28625"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_113519630.1"
                     /translation="MIRLKKVFNLTEEEIVLTNEIAEAISKEPTVETHEVNINFIDVLP
                     IIPNPHNLGLSKDIDPLSLIYSKFITQTDIPPALPVFSFLSYLSAFSVNNNIMYKHPTS
                     PADYLNTWTLILAPSGAAKTTAAKIISSSIPKDIEENPMIKPNFEGADGSAAFISELAK
                     AEKKIDNFGKPIQPIFWIEDEYSQFMKKLMPGGSMVETRKTMLKIHDNDKARRVTKNDT
                     IETESIVMSGLFLNTIDSFARNFDQESINDGLGRRHNFVYAERGEKVVPTWTVDQIIES
                     LKEGLDDFFTTVKTNVIYTYSPECRKIYDHFYMVYKEKFDHILGEETNGTFFRTYFMLS
                     WKYAAIYHILLKQEGTEIQAKSFDYGIKVSLMFLSSIKRFLDYKVDLKSGAKQVRKEYL
                     QKAAEKELNNLQKYKEFLLDNIEISLRDFNRKFNIKKIESLKILKTIRENKLIKYHPLF
                     EVLAKEEAKAKK"
                     /name="hypothetical protein"
ORIGIN
        1 ggacaatggg tttatcgtca tcaaacaaat cagatttaat tgatatttga ttttttcgta
       61 ttctgtttat tttaacacct ttcatatttt caatatattt gatggtttgt tctctatttt
      121 ccaactcttc attaataatt aatgccatta tttcagtatt aatattttca ataaattgtt
      181 ttttatcttc tggaatattg gtttttggtt tgttggttaa tgtgttttta ttttctttct
      241 cttcgaatgg acttgataaa ttattttcat aattaattat ttcagagtag ttatttacta
      301 atccacgatc tcttttatct aaatatacag tgtatgatct tctgtcttgt aaactgttaa
      361 atgttaaata atttaactct accctgccct tgtcgttgtg ttctacccac ataactggag
      421 gtctgttttc ttgttcaatc cctgcaaata aaatatcttc aaactctgta attaatttag
      481 tttttaattc ttctgatatg gatttttctt caaatgataa aacaccacac acatatgggt
      541 ttgatttgtt tttatctaaa atataatttt caaaattgat taggtcttgt tttgtggttg
      601 ttgatagtat ttttatgtct ttctgttgtt ctgggttttt ctctctcaat aaatattcaa
      661 tacttttttt tatatttctt tgacctttac tttttttacc tcctattatt ttatggtaca
      721 tattatattc tcctttttat ttcttctatc tcatttgtta tttcctcaaa caaatttaaa
      781 aaatcttctg aaaactcatg ggaaataaat ttatcaatat cagtatttat tgctcttgca
      841 atctggttaa tgtttactcc tattttatgg agttcaaaat atacatcacc agttttattt
      901 tttgctattt cttttttgag tttaaaatct ttattgagaa taagatctct aacggctgtt
      961 gctctgggtt ttttcaacat agaagccagt tcttcaacct gtgcaaattc ttcatcattg
     1021 aagcacaggg ttatgctgtg cttcctgttg tatctatcct tatagctcat cctatgcctc
     1081 ctgtgttggg ttttaagggg attgaagggg gaacccctca caagcgacaa tacgaagtat
     1141 tgtctgagct tgctgttata tagttttaca cttatatatt aattaataca tataaaaatt
     1201 aagtcaatac ctgaataaaa aataatcaaa tcaaatttgg gttttatata taaccgtgaa
     1261 caaataaagc cattttacaa atggcttttt tgtgaggggt tgacctgtcc ggtaggacgg
     1321 ttttattttt ttgctttggc ttcttctttt gctaacactt caaacaatgg atgatatttt
     1381 attaatttat tttctctgat tgttttaagt atttttagac tttcgatctt tttaatatta
     1441 aattttctat tgaagtctct taatgaaatt tcaatattat caagcaaaaa ctctttgtat
     1501 ttttgtaaat tatttaattc tttttctgct gctttttgta aatattcttt ccttacttgt
     1561 ttagcaccag attttaaatc tactttataa tctaaaaacc tttttatact ggataaaaac
     1621 attaatgaaa ctttaatccc ataatcaaaa gattttgcct gaatctcggt tccttcttgt
     1681 tttaataaaa tatgatatat tgctgcatat ttccaactca acataaaata agttctgaaa
     1741 aatgttccgt ttgtttcttc tcctaatata tgatcaaatt tttccttata aaccatataa
     1801 aaatgatcat aaatctttct acattctggg gaatatgtat agattacgtt tgttttaaca
     1861 gtagtgaaga aatcatctaa tccctctttg agagattcga tgatctgatc tactgtccaa
     1921 gttgggacaa ctttttcccc tctttcagca taaacaaaat tatgtcttct tcctaaccca
     1981 tcattaatac tttcctgatc aaagtttctc gcaaaactat caatagtatt caaaaataaa
     2041 ccactcataa cgatactttc agtttcaata gtatcgtttt tcgtaactct acgggcttta
     2101 tcattatcat ggatttttaa catcgttttt ctggtttcaa ccatagaacc tcccggcatt
     2161 aattttttca taaactggga gtattcgtct tctatccaga aaatcggctg tattggtttt
     2221 ccgaaattat caattttctt ttctgctttt gccaactctg aaatgaatgc tgccgaaccg
     2281 tcagcacctt caaaattggg tttaatcatt gggttttctt ctatatcttt tggtatagag
     2341 cttgaaataa ttttggctgc tgtggttttt gctgcacctg acggtgcaag tattaaagtc
     2401 caagtattca agtaatccgc tgggcttgta gggtgtttat acattatatt attattaaca
     2461 ctaaaagcac ttaaataact taaaaaagag aatacaggta aagcaggtgg tatatctgtt
     2521 tgagtaataa acttagaata tattaaagat aatggatcta tgtcttttga taaacctaaa
     2581 ttatgggggt ttggtattat aggtaaaacg tcaataaaat ttatattcac ttcgtgggtt
     2641 tctactgttg gctctttgct aattgcttct gctatttcgt ttgttaaaac tatttcttct
     2701 tctgttaaat taaaaacttt ttttaatctt atcataataa aatctcctat tatatctatt
     2761 atataattag ttataacata aggttttaac ttgtcaataa tatttttaca aaaaaatatg
     2821 acacattcca aaatgtctta ttttttggtt ggtttttgcg aagccaaaaa cataccaccc
     2881 cccccctgtt tgacactatg acattatgac aaatcacgta acatactgat tttaaagggt
     2941 taaaaaaaat ttttgtcata gatttgtcat aaaaaatcta acaacctttg acaaaagaga
     3001 agacattttt cgtaatgtat tgatgtataa agaaaaaata atctttctcg gtgtcattag
     3061 ttttatgaca aaatcaaaaa ttaaaaaaac aggcaatttt ataacctgtt ttgtcataaa
     3121 atatcccgtt tttatttttg actcaatgga ttttaaggtg tcggatcttt tttggaaaat
     3181 atctgacaag ttttcttggt tttttctgac actctgacag atttctgaca gaaaaccgtc
     3241 ctaccggaca ggtcaacccc tcacaagcaa gaaccagcct gacggctggc tctcacttgt
     3301 tcacggttaa agttttatca tagtgatcta gtagtgttgt catcatattt ttaatatggt
     3361 ttatatcgtc ttctgttgta acttccctga tttttgattt tgagtttttc ttcttcgatt
     3421 tttcgtcttt tttcgaattt ttgtctgtgt tcattcttcc ttgtatatgc tgaaaacgtt
     3481 cgtatgtatc ttccaaaagt agaaataatt gagtgtacag atcttctaca tactccaaat
     3541 cttccctcag ttgtatcaat cgactttctg aattgctgtt gttttcttcc agcttcttca
     3601 agtctcgaat taacaaattg ttgttgctga ttaatttttt caatatctct tgttcttgta
     3661 gttcctgctg tttcttctgt tgctgtttta gttttatatt ctgtattaat tgactcatta
     3721 tttacaatct ccatattatc agtgcgaata tacatacctg aaaaaccatc gttattacta
     3781 acattgataa aatcgccgtt agtatattta tatccaatat cttgatcttc tctttgaaca
     3841 ctttgtctat tttctggtat atttttgaac gatggttttt taaactttga tctatttctg
     3901 atttctcgtt tttcaattct ttctgcaaat gttcttttaa gctctgtgag tttttctcta
     3961 acaaactctg gatctcttgt aggttttggg ttgaattctt ttctataatc gctgtaatct
     4021 cttccctctt catatatatc tcctttca
//
