LOCUS       NZ_CP083795             3514 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella variicola strain INF352 plasmid pINF352_5, complete
            sequence.
ACCESSION   NZ_CP083795
VERSION     NZ_CP083795.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357250
            Assembly: GCF_020149805.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella variicola
  ORGANISM  Klebsiella variicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Hawkey,J., Cottingham,H., Tokolyi,A., Wick,R., Judd,L.M.,
            Cerdeira,L., de Oliveira Garcia,D., Wyres,K.L. and Holt,K.E.
  TITLE     Linear plasmids in Klebsiella and other Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2021) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:10:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,921
            CDSs (total)                      :: 5,798
            Genes (coding)                    :: 5,645
            CDSs (with protein)               :: 5,645
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 52 of 153
            Pseudo Genes (incomplete)         :: 99 of 153
            Pseudo Genes (internal stop)      :: 23 of 153
            Pseudo Genes (multiple problems)  :: 19 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083795.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Klebsiella variicola"
                     /mol_type="genomic DNA"
                     /strain="INF352"
                     /db_xref="taxon:244366"
                     /plasmid="pINF352_5"
     gene            236..427
                     /locus_tag="LB484_RS29565"
                     /old_locus_tag="LB484_29565"
     CDS             236..427
                     /locus_tag="LB484_RS29565"
                     /old_locus_tag="LB484_29565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071008427.1"
                     /translation="MLKKNEIILIKSCGNSKTKIFLRGVDEGLMIDASFEDIKFAINNN
                     LHDLLGIQAETTKLKLTK"
                     /name="hypothetical protein"
     gene            complement(841..1056)
                     /locus_tag="LB484_RS29570"
                     /old_locus_tag="LB484_29570"
     CDS             complement(841..1056)
                     /locus_tag="LB484_RS29570"
                     /old_locus_tag="LB484_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1063..1560)
                     /locus_tag="LB484_RS29575"
                     /old_locus_tag="LB484_29575"
     CDS             complement(1063..1560)
                     /locus_tag="LB484_RS29575"
                     /old_locus_tag="LB484_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1601..2299)
                     /locus_tag="LB484_RS29710"
                     /old_locus_tag="LB484_29580"
                     /pseudo=""
     CDS             complement(<1601..2299)
                     /locus_tag="LB484_RS29710"
                     /old_locus_tag="LB484_29580"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2238..2579)
                     /locus_tag="LB484_RS29585"
                     /old_locus_tag="LB484_29585"
     CDS             complement(2238..2579)
                     /locus_tag="LB484_RS29585"
                     /old_locus_tag="LB484_29585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(2665..2837)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.655"
                     /coverage="100.0"
                     /evalue="9.54e-56"
     Typing          2762..3308
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(726..2248)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2245..2558)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="9.860000000000001e-59"
ORIGIN
        1 aggctgaatg aatctcaaga tttcatttag cctgccaccc gatcattcaa agttttggcg
       61 tagttctgac ccgctccccg tgatttgtta catacccacc acagggcatt agttttttta
      121 tttttttgat gatatgtaca aaatcagtgg ttaggaataa tcttagcagc cttaacaaac
      181 acacacaaaa acaacgataa tgattcatct gacgacaaca aacaatgaca atgtcatgtt
      241 aaaaaaaaat gaaattatct taataaaatc atgtggtaac tcaaaaacaa aaattttttt
      301 acgaggtgtg gatgagggac tcatgattga tgcaagtttt gaagatataa aatttgctat
      361 caacaataac ctccacgatc ttctcggaat ccaagccgaa accacaaaac taaaactaac
      421 aaagtgatac agcccctgaa gcatgaagga tcgaaataaa gccaccgatt taccataacg
      481 tcttcttacc cgcaccgcgc cggaggcact cagggcgttt ctgatggctg acgggcaaaa
      541 ggtggtggca agtacaccga aaaccaccag aaacgcacct gatttatttt acgcatatgc
      601 atcacttatt cacatcactg catagccggt gatttcggtt aaaaaagact ctctgtgtgc
      661 tgaaatcgca gaaaatggcc atgatccccg tcacgctcag gccacctgag acgggcgttt
      721 atcagtctcc atactccctg tcaccatgag ataatgtgcg ttgccgttcc ggtaccagat
      781 ctgctgtctt ctctggttcc ttttccgttg tttgcccgta tcgctgttgc tcaatctcat
      841 tcagcctgtc gataaccggc gcagctgctc gctcaaactc tgcacctgcc tgctcaagcc
      901 cgacacgcgc tcgctcagcg ccgcgttctc ccgttgcata agaccagaca tcttccgcca
      961 ttcctcgaag gcgctctccc actcgtccag ccttctcgag tagtcctgct gtagctgctc
     1021 taacgcgctc agcaattgtt tttccagttc cgtcattctg ctctactccc gtaccagctt
     1081 caccgcgttc cgcgtcttgt tgtccaccgt ccggcccgtt tcggctttca tcccctccgg
     1141 cagcaccaga aaccgtccgt tgcggttctc cagataggtc acgccccacg tctgcatgtg
     1201 cagtttcgac agcgtatctc tctgctgcct gatttccgcc aggttcgccg ctatccggtt
     1261 cccctggtac cacagcaccc cgctcaggct ggcgaacagc agccctgaca ccatggtgat
     1321 ggtcagccac gtccgcccga ccattcgcag cacgctcagc cggttcgact gcatcgccgc
     1381 gttcatcccc tgttcgtggg cgctgatggc gtcgttgatt ctcttcgcgc ttgcgttcag
     1441 ttccgatttg acaacgttct cgtgcttgct gaatgcggct ttcagcctct cttcggtaag
     1501 ctgctgctgt gctttcgatt tctcctcgaa gtccctcgag gccgttaaaa gactgctcat
     1561 aaatggctcc cctcagtcgg atatttcgcc ccccgtccgg gtcggcaatg ctgatactgt
     1621 tttttgtggt tctgaccacc tcaaaaccgg ccgctgtgag cgcccctgtg acgtcatcac
     1681 gcgttttcag ctctcctgat gaggctaaca tcagcaaacc ccgtgtaatc gtctctgcgg
     1741 cttcctgctt cgtttctggc agtcctgaag gcgtgaccag cgcccgccgg ttctccgggt
     1801 cgttcggatc atgtagcccc agcctgccgt tgaccactgt ctgccacgca tcgatgcgcg
     1861 gccggtcggc acgatcgtaa tacggctgga ggcgtttccc cgtcagtaat tcggtattgg
     1921 ggatcagaaa attcagctca agacgaccct tgtctgcgtg ttccacccac agaatgctgt
     1981 actggtcttt atcgagtccg ggcatcagaa cccgctcaaa gctggccatc agtttttcac
     2041 gctgacccgg cggcaggtcc gcctccgcga atgacagcac cccggaggta tattttttca
     2101 catacggcga cgcatcaatc agctcccgga cttcttccgg cttcccctgc aggacgctgg
     2161 caccttcacg ctgccggtct ttacccagaa gataatccac aggaccggcg cccccgccgc
     2221 gaccccgggg atggaattta acaatcatcg tccggccctt tttccagtac ggcatgccgc
     2281 aaccgttcca gaccggcatc gatcgccatc agcgctgcca cgatctgcac ccggtcatga
     2341 ccggcaccac cggcgttcac tttccgggcg atctggttca ggttattgcc catgccggca
     2401 agctgacgca gcagcgcggg cgaaatcgag ggaagcctgc cggcgcgtgt cggtttctca
     2461 tcaaggcagg tctggcgcat ccacgccgcc agttgttttc cgtcgcaccg ctcaagcaac
     2521 cgccggtgtt cgtcttccgt cacccacatc gtgagcatct tgttgcgttt atctgccaga
     2581 atcgcctcct gataacgccg tgacggacgg gaaagccttt cccggtcggc gcggggttgg
     2641 acaagccgca ggcgcgtcag tggggtttcg gggcgcagcc ctgaaccagt cacgtagcgc
     2701 tagcggagtg tatactggct tagtatgttg gcatgagtgc gataatcagg aaagcgcctt
     2761 atgcggcaga agaaaaatgc tgcagcagag cgtaagcaga atatgcgata cagaagatat
     2821 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtgccg
     2881 gcttacgggc ggggcggaaa tttccaggaa gataccagga agaaacttaa cagggaagcg
     2941 atgaggccgc ggcgaagccg tttttccata ggctccgccc ccctgacaag catcacaaaa
     3001 tctgacgctc aaatcagtgg cggcgaaacc cgacaggact ataaagatcc caggcgtttc
     3061 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     3121 cgctgttatg gccgcgttta tctcattcca cgcctgacac tcagttccgg gaaggcagtt
     3181 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgactgctg cgccttatcc
     3241 ggtaactatc gtcttgagtc caacccggaa agacacgcca aagcgccact ggcagcagcc
     3301 actggtaaca ggattagcag agcgagttaa tgtaggtgat gctacagagt tcttgaagtg
     3361 gtggcctaac tacggctaca ctggaaggac agtatttggt atctgcgctc tgctgaagcc
     3421 agttacctgg ttaagcagtt ccccaactga cttaaccttc gaaaaaccgc cctgcaaggc
     3481 ggttttttcg tttacagagc aagagattac gacg
//
