LOCUS       NZ_CP083783             2892 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF118 isolate INF118 plasmid
            pINF118_8, complete sequence.
ACCESSION   NZ_CP083783
VERSION     NZ_CP083783.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357001
            Assembly: GCF_020149875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Hawkey,J., Cottingham,H., Tokolyi,A., Wick,R., Judd,L.M.,
            Cerdeira,L., de Oliveira Garcia,D., Wyres,K.L. and Holt,K.E.
  TITLE     Linear plasmids in Klebsiella and other Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2021) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 147x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 15:42:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,870
            CDSs (total)                      :: 5,748
            Genes (coding)                    :: 5,595
            CDSs (with protein)               :: 5,595
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 67 of 153
            Pseudo Genes (incomplete)         :: 95 of 153
            Pseudo Genes (internal stop)      :: 23 of 153
            Pseudo Genes (multiple problems)  :: 26 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083783.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF118"
                     /isolate="INF118"
                     /isolation_source="wound swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pINF118_8"
                     /geo_loc_name="Australia"
                     /collection_date="2013-07"
     gene            complement(join(2567..2892,1..781))
                     /gene="mobV"
                     /locus_tag="LB478_RS29365"
                     /old_locus_tag="LB478_29365"
     CDS             complement(join(2567..2892,1..781))
                     /gene="mobV"
                     /locus_tag="LB478_RS29365"
                     /old_locus_tag="LB478_29365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791318.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_064354081.1"
                     /translation="MYSIIRKQSHKKTAIQAVHNHNMRIVIEENVNSKKSNLNEILIGT
                     ENTRSDIMSYIKNNDIVIRNKDTVIFNEFVLTASPEFFFNNKDGSKKSRSEYKSNLKDW
                     VKTQIDFLEKENYGKCVNAVLHLDESTPHIHALILPIVDNKLNNKSFWRGKNSYGRLQD
                     IYNTANSKFGLKRGEEKSKTLVDHTTLKDYRDLIRQDKDEENEFYNELSKNILDTPPRK
                     NFIGLEKKYSAEEVKEIASATFKKLNRQRRRAKFRAKKSDEKADAATSEFYKKKKETSK
                     IEFFYNENQKQLEKISVENSTLKTHNLELKQSQSDLDLVRKFLPMELQSLVVRARELAS
                     GGDSGGTKHTAEPTPTADLTTYKRPKYK"
                     /name="mobV"
     gene            1798..2550
                     /locus_tag="LB478_RS29370"
                     /old_locus_tag="LB478_29370"
     CDS             1798..2550
                     /locus_tag="LB478_RS29370"
                     /old_locus_tag="LB478_29370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000155158.1"
                     /translation="MTIQEVKKPRVRTKKENDKLTEYKMNPFVFEKELKIETKTRNLTV
                     GRNTELVRKDEKDDSEESESYFTNIIQQKEVDKEEFIKLFTSQIKVYFDLTKTAYKIFL
                     IILSLYQKEIGKDYVLLTCKKAQNIAKTLDFELSSPIFYRGIKELIEKKIIAKSVDKIV
                     FYINPAIFFNGDRARFVTEVIKKKEEIEKNQLLQELEDTSKNKDFDVNETDDSVENLDL
                     MIARLQKKKEELERKEKFGNWQDLEDAS"
                     /name="hypothetical protein"
ORIGIN
        1 attttttagc cctaaatttt gcccttcttc tttgtctatt tagcttttta aaagtagctg
       61 aagcgatttc ttttacttct tcagcagagt attttttttc taaaccaata aaatttttcc
      121 ttggtggagt atccaaaata ttttttgaaa gctcattata aaattcgttt tcttcatctt
      181 tatcttgtct gattaaatct ctgtaatctt ttaatgttgt atgatcaact aaagttttac
      241 ttttttcttc accacgtttt aaaccaaatt tactatttgc agtgttgtaa atatcctgca
      301 atcttccata agaattttta cctcgccaaa aagatttatt gtttaatttg ttgtcaacaa
      361 taggtaaaat taatgcatga atatgtggtg tactttcatc taaatgcaaa actgcattaa
      421 cacatttccc gtaattttct ttttccaaaa aatcaatttg agttttaacc cagtctttta
      481 aatttgattt atattcagat ctagatttct ttgaaccatc tttattatta aagaaaaatt
      541 ctggtgatgc agttaaaacg aattcgttaa aaattactgt gtctttattt ctaatgacaa
      601 tatcattatt tttaatataa ctcattatgt cagatctggt attttccgta ccaattaata
      661 tttcattcaa atttgatttt ttagaattta cattctcttc tataactatt ctcatattat
      721 gattatgtac agcttgtata gctgtttttt tgtgtgattg tttcctgatt attgaataca
      781 tttttatttc tccatattaa tatacaaccc ccattttttt tggataaatc caatattatg
      841 agaatgtcga atacattctc atgtggcgtt aaatgccacc tgtgcagctg tgtaaggttt
      901 tgacgttgaa ccggtgcaaa ccggttcggg gcttgggtgg gctatgcccc ccaacgaacg
      961 actgagcgta gctcggtcta gtgagtgata gaccgaaaag taaactcaac gtatatattt
     1021 aaagttatca ctttaatata ttctgtcaaa tagctaccgc ctcgcctaac ggctcgttgc
     1081 tgaatcctct ttaaagcaaa agtaaacttt tactttaaaa aggattgatc acaataataa
     1141 taaaatacat gaacaaaaaa gacgggttgc gatgtgggca agttcttgac cactcgcagg
     1201 ggtgggggga cccggaacaa gagtacttgt tctggtgggg ggaacgggtt taggggttcc
     1261 ccccagtagc tgtagaacgc tctctaacgc tctgtgacgg gtttttgtag ttactttaac
     1321 cgtgagagtc cacgtacagc ttgctgtatg tggattcgag gggttggcca gcccggcgag
     1381 ggcggttttg gttttactct tagatcttat atttattaat tcttgcagaa aaactcaaat
     1441 cataaaactc ctctctaagc cttggtacgt ctggctttgc tccctttttt ctaaaaaaca
     1501 aaaagtataa agttttatac tctatcctta aaaaagtaac tctagagtaa taagttttat
     1561 actctagagt aataaacttt atcgttttga ccccataaaa acgataaaca ccataaaacg
     1621 agtaataaac tttatcgttt tgaccccaaa aaaacgataa atacactaaa atttctagaa
     1681 ttttatttat cgttttaatc attaaaaacg acattttttt atcgttttct attgacatat
     1741 taaaaggatc agttataaca ctaatatcaa gtatctaaat ttaaagagga ataagttatg
     1801 acaatacaag aagtaaaaaa accaagagtt agaacaaaaa aagaaaatga taaattaaca
     1861 gaatataaga tgaatccttt cgtttttgaa aaagaactaa aaattgaaac aaaaacaaga
     1921 aatttaactg taggaagaaa tactgaatta gtaagaaaag atgaaaaaga tgattcagaa
     1981 gaatcagaat catattttac taatattatt caacaaaaag aagttgataa agaagaattt
     2041 ataaaattat ttacatcaca aataaaagta tattttgatt taacaaaaac agcatataaa
     2101 attttcctta taattttgtc tttatatcaa aaagagatcg gtaaagatta tgttttattg
     2161 acatgtaaga aagctcagaa tattgctaaa acattagatt ttgagctttc gtcaccaatt
     2221 ttctatagag gtataaaaga acttattgag aaaaaaatta ttgctaaatc tgtagataaa
     2281 attgtttttt atataaaccc tgcaattttc tttaatggtg atagagcaag atttgtaact
     2341 gaagttataa agaaaaaaga agagatagaa aaaaatcaac ttcttcaaga gttagaagat
     2401 acatcaaaaa ataaagattt tgatgttaat gaaacagatg atagcgttga aaatttagat
     2461 ttaatgattg caagattaca aaagaaaaaa gaagaattag agcgaaaaga aaaatttgga
     2521 aattggcagg atctggaaga tgcatcataa aagcccatca gggcttttat ttgtatttag
     2581 gtcttttata ggtagttaga tctgcagttg gtgtaggttc tgcagtatgc tttgtaccgc
     2641 cagaatcgcc gccagaggcc agttctctgg ctctgacgac aaggctttgc agctccatag
     2701 gtaaaaattt tctcaccaga tctaggtcac tttgtgattg cttaagctcc aagttatggg
     2761 ttttaagtgt ggaattttcc acagagattt tttccaattg cttctggttt tcattataaa
     2821 aaaactcaat tttcgatgtc tctttctttt ttttataaaa ttcacttgta gctgcatcag
     2881 ctttttcatc ag
//
