LOCUS       NZ_CP083782             3549 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF118 isolate INF118 plasmid
            pINF118_7, complete sequence.
ACCESSION   NZ_CP083782
VERSION     NZ_CP083782.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357001
            Assembly: GCF_020149875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Hawkey,J., Cottingham,H., Tokolyi,A., Wick,R., Judd,L.M.,
            Cerdeira,L., de Oliveira Garcia,D., Wyres,K.L. and Holt,K.E.
  TITLE     Linear plasmids in Klebsiella and other Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2021) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 147x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 15:42:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,870
            CDSs (total)                      :: 5,748
            Genes (coding)                    :: 5,595
            CDSs (with protein)               :: 5,595
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 67 of 153
            Pseudo Genes (incomplete)         :: 95 of 153
            Pseudo Genes (internal stop)      :: 23 of 153
            Pseudo Genes (multiple problems)  :: 26 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083782.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF118"
                     /isolate="INF118"
                     /isolation_source="wound swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pINF118_7"
                     /geo_loc_name="Australia"
                     /collection_date="2013-07"
     gene            complement(657..1079)
                     /locus_tag="LB478_RS29335"
                     /old_locus_tag="LB478_29335"
     CDS             complement(657..1079)
                     /locus_tag="LB478_RS29335"
                     /old_locus_tag="LB478_29335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1079..1270)
                     /locus_tag="LB478_RS29340"
                     /old_locus_tag="LB478_29340"
     CDS             complement(1079..1270)
                     /locus_tag="LB478_RS29340"
                     /old_locus_tag="LB478_29340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1994..2149)
                     /locus_tag="LB478_RS29345"
                     /old_locus_tag="LB478_29345"
     CDS             complement(1994..2149)
                     /locus_tag="LB478_RS29345"
                     /old_locus_tag="LB478_29345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(2257..2514)
                     /locus_tag="LB478_RS29350"
                     /old_locus_tag="LB478_29350"
     CDS             complement(2257..2514)
                     /locus_tag="LB478_RS29350"
                     /old_locus_tag="LB478_29350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            complement(2516..>3118)
                     /locus_tag="LB478_RS29355"
                     /old_locus_tag="LB478_29355"
                     /pseudo=""
     CDS             complement(2516..>3118)
                     /locus_tag="LB478_RS29355"
                     /old_locus_tag="LB478_29355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="klebicin B-related nuclease bacteriocin"
                     /name="klebicin B-related nuclease bacteriocin"
     gene            <3117..3458
                     /locus_tag="LB478_RS29360"
                     /old_locus_tag="LB478_29360"
                     /pseudo=""
     CDS             <3117..3458
                     /locus_tag="LB478_RS29360"
                     /old_locus_tag="LB478_29360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          1319..1785
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.979"
                     /coverage="92.0"
                     /evalue="2.73e-170"
ORIGIN
        1 aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt tttacggcat gcatgagtat
       61 gcgcgcaagt gcatagcgcg gaaattcacg ccattcagcc agggtaagtc ctgtttccgg
      121 cgttcttctg atccgaaaac ggtgaacggg gacgcaattt attgcgtggc cgtgaggcgt
      181 tgccggcgca gccggaaaac cggttttcag cacgttcagg aatcgggttc gtcgacggcc
      241 ggcaatcacg ttatggcaag tcgccggacg actcaccaca cgattcaccg gcgattcgaa
      301 tcgtgaattg aatcaagagt caccatgaac aaaaaacgcc caaattgatg aattgaatca
      361 agagtcgatt catcataaca acatgataat aaagcgtgat tcacatatga atcgtgggca
      421 attcgtaaac gattcgccat agagtcgtcc acgacttgga tctgcacttt gtaacacatc
      481 actgcacatt aatttttcat taaccaattg ttttaaataa ggaatctgca aaattttaat
      541 tttgcgtagt gtgtgggtat ttttagtcgt gaggagcgtt cagagatctg gcgatttttg
      601 ggaaggttca ggaactccct gggatggccg gagaggaaaa ctcaagcatg gcaggatcag
      661 gattttttcc agaatttcca ccacggggaa tggggtggtg aagaaggctc gcgcttgtgc
      721 tccagaagct gcatagcctg acgaagggaa tcaatatgct catctcgagc cttaacagcc
      781 cctttaagac catcgttttc agcttcaagt agagcgattc tatgttgtaa atcatgtaaa
      841 ccaacgctgt taatacttgt agattctgcg tcacttttta catcatattt tacagtatcg
      901 atgacaccat caagagaacc aaaaactcta ataagctctg aagtatcaat aactttaata
      961 gccttcccgt gctttacaac agaggtgtaa cttaacttcc cactgttaat catcttgtaa
     1021 attgtgggac ggctaacctt aacaagccgt gccgcttcac taatagacac tctggccatc
     1081 aattgccatc ctgtaattca tccagtctcg aggacagtgt acggaaaaga tgctccgcat
     1141 catcgtggag tttctcacaa aggtcggcct caacatcgag atcaagctcg ttaagcttct
     1201 ccagcaggag cagagactga ttctggatga atttagccat gttcagcgct gttttctgtt
     1261 gtttcgtcat aaagggcctc agagcgtcag tgctcaatgt cagacagatc tcttttccgc
     1321 ttcctcgctc actcagtcgc tacgctcctg ccgttcggct gcggcgagcg gtcgcaacgc
     1381 gcacataacg acatagagca gacagctcgg agagggagaa ggcagcacag cggccgtttt
     1441 tccataggct ccgcccccct gacaagcatc acagaatttg acgctcaaat cagtggtggc
     1501 gaaacccgac aggactataa agataccagg cgtttccccc tgatggctcc ctcgtgcgct
     1561 ctcctgttcc tgccattcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc
     1621 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac
     1681 gaaccccccg ttcagtccga ctgctgcgtc ttatccggta actatcatct tgagtccaac
     1741 ccggaaagac acgacaaagc gccactggca gcagccactg gtaacggatt tagaggagtt
     1801 ggtcttgaag tcatgcgcct gttaaggcta aactgaaaga acaagttttg gtgactgcgc
     1861 tcctctaagc cagttacctt ggttcaatca cctgcaagtg tttgaacctt cggaaaaacc
     1921 gccctgcaag gcggtttttt cgtttacaga gcaagagatt acgacgatcc aaatgggatc
     1981 tcaagaagat cctttacgga ttgccgttcg taccgctatc aagcgtgact ccggtcaccg
     2041 tcgatgaagg tgaaacagcc ccacctccgg tgtctcgaac attattgacc tgacatgctg
     2101 ccagaaccac agaaacggta acaaccccta ggaaaagtaa ttttttcata tatcaccttt
     2161 tatttttatt attctaggca aatggatatc tagtaattat tcttcaaatc cagagttaca
     2221 aatgagcggg ctcataactt gaacccgcaa attcaatcac gcttttttaa aacctggctt
     2281 accgttagca gctcgccatt cctttacggt tttaacaatt cctttcgccg aagaatcttc
     2341 gcctacctta ggcttgaaga tgaggttcca accagcagga tgctctgtta gttgggcgaa
     2401 cgaataaata gcatcatcgt tctctttgtc agttggaaaa tcagcagcga ttattttact
     2461 aacaaaagca gtaaattctt cttccgtaaa atctgaaatt ttattaccag ccattttatt
     2521 ttcctttatg tgtctcaata tgatttttag gtgttagcgc cttaatgtta tcgacatcca
     2581 taacagcccc tccttgagat atttcttttt cgtggtgtaa ttcaatttta acacgtttcc
     2641 ctactgattc taaaaagtcc gcagtaggtg cccttccagc tttcatctga ttaatatcag
     2701 cttcactaaa ttgcttgctc agtgcagaat cgtcagcaac cgctttccag aacgcccgac
     2761 ggaaagaatc aaaactaccg aaagtcttcc cgcgtaattt atccgcaatc tgggaaggaa
     2821 caggagcacc atccccggta ctggcccccc ccatccagtt attgccaacg ttctgccctt
     2881 tgccgctcac tgtaccgggc atgttacgag ggctacggta catcacatac agcggtttta
     2941 ggccggattc cggaggaaac accagaataa tatcgtcaaa atcgaggtca tccgctaccg
     3001 gcgtcacggt tttatctgta gcctgtggcg ccgaaatctg atcaccggta tgcagaatac
     3061 gctccggtag tggtaccggc ccgcttagcg tcagtggtcc gtttgcacca ggtgcgtcaa
     3121 gtgcactaga tttcaccagg atggcgctta atcaggaaaa atacctcgat gactttacat
     3181 ggaaagaact gaacgacgtt atcaacttca tacgcaaagc agaaggtagt gcatctgacc
     3241 acagctatgc actccagtta ctggagatta atttcaaggc aaacccactg gatctcatct
     3301 atcaccctga ctgctggttc aatgatgaag cattgtttca tgcccggctg actaccgaag
     3361 aaattggtgg ctacttaatg aaaaaatcag gtcgatggct caacgatgca ccggatatcc
     3421 aactggtgta tgccatcccc caggatgttt atgattaacc aagtcaccat ttaccataat
     3481 cccttaattg tacgcaccgc taaaacggcc tcaggacgtt tctgacggga aaccggtaaa
     3541 acaggcaac
//
