LOCUS       NZ_CP083780             4740 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF118 isolate INF118 plasmid
            pINF118_5, complete sequence.
ACCESSION   NZ_CP083780
VERSION     NZ_CP083780.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357001
            Assembly: GCF_020149875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Hawkey,J., Cottingham,H., Tokolyi,A., Wick,R., Judd,L.M.,
            Cerdeira,L., de Oliveira Garcia,D., Wyres,K.L. and Holt,K.E.
  TITLE     Linear plasmids in Klebsiella and other Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2021) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 147x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 15:42:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,870
            CDSs (total)                      :: 5,748
            Genes (coding)                    :: 5,595
            CDSs (with protein)               :: 5,595
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 67 of 153
            Pseudo Genes (incomplete)         :: 95 of 153
            Pseudo Genes (internal stop)      :: 23 of 153
            Pseudo Genes (multiple problems)  :: 26 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083780.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF118"
                     /isolate="INF118"
                     /isolation_source="wound swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pINF118_5"
                     /geo_loc_name="Australia"
                     /collection_date="2013-07"
     gene            join(4677..4740,1..1493)
                     /locus_tag="LB478_RS29280"
                     /old_locus_tag="LB478_29280"
     CDS             join(4677..4740,1..1493)
                     /locus_tag="LB478_RS29280"
                     /old_locus_tag="LB478_29280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_048290311.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMSGYIEMAQGELSYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGYVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGEFFFSGNRYLLGYAHMKNTAWNVLVYSHADSATTILITYAKS
                     VLVLLIVIIITFSLLSYFIASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTHVKVMMERVDSLNEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFF
                     KKINDSFGHGVGDEVLVSLAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAER
                     IRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            2534..2875
                     /locus_tag="LB478_RS29285"
                     /old_locus_tag="LB478_29285"
     CDS             2534..2875
                     /locus_tag="LB478_RS29285"
                     /old_locus_tag="LB478_29285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3084..>3512
                     /locus_tag="LB478_RS29440"
                     /pseudo=""
     CDS             3084..>3512
                     /locus_tag="LB478_RS29440"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3553..4050
                     /locus_tag="LB478_RS29295"
                     /old_locus_tag="LB478_29295"
     CDS             3553..4050
                     /locus_tag="LB478_RS29295"
                     /old_locus_tag="LB478_29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_020325392.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLQMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            4057..4272
                     /locus_tag="LB478_RS29300"
                     /old_locus_tag="LB478_29300"
     CDS             4057..4272
                     /locus_tag="LB478_RS29300"
                     /old_locus_tag="LB478_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2268..2440
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.126"
                     /coverage="100.0"
                     /evalue="1.99e-77"
     Typing          complement(1791..2343)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.639"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          2866..4388
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.409"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taacttctat ttttctgatt gccgttatta tggtttttca gcgcgaaaat atagaaaaag
       61 atttgcttga tgctaattca tcgtacgcta tgaaaatgtc agatgtaatg agtggttata
      121 ttgaaatggc tcaaggtgaa ttatcatatg gagcaaaaaa aataaaatca actaatgata
      181 ttgactcact taaagatgag gcggataggt taagattgca gtctggtatg tttaattcag
      241 tttttgttgt tagtaatgat gctgttgttc tggcgacttc gccagaatct cttgatttag
      301 ttggtacccg cttgaattca agtgccagca aattggcgat tgaggaaaag aaaccattca
      361 tatcttcacc gttcaaatca gttactggga atttgattat tttgttgtca catcctattt
      421 atgataagaa tggcaattac atagggtatg tcggtgggtc tatatatcta aaaaaacata
      481 gtttatttag cgatgttttg agcaggcatt ttttcaagag cgacactgaa attagcattg
      541 ttagtgatga tggaactgtg atctttaata acgataacag tgctgttggt cggcccatgg
      601 ttatgtctga tgatttgaaa tcaaagttaa cggagtctga tagaggtgag ggggaatttt
      661 ttttttcggg gaatagatat cttctgggtt atgctcatat gaaaaatact gcttggaatg
      721 tgttggttta tagccatgct gacagcgcga ctactattct tattacttat gctaaaagtg
      781 tacttgttct gctaattgta ataattatta cattttcact tctttcatat ttcattgcct
      841 cccgtatttc acttccttta gaaaagcttg ctatatctac caatgctaaa gatatagggg
      901 cttcgttagt ttatattaaa gctatcaatg cttggtatgc cgaagctgat cgacttaaaa
      961 aggcactttt tacgcatgtt aaggtaatga tggaaagggt tgattcttta aatgaggtgg
     1021 cgcttaaaga tccccttacc ggcgtgaaca atagattggg gttttcaact aaaatacaga
     1081 ataacaagta tggggcagga gcttcagtta ttgctattga tgttgatttt tttaaaaaaa
     1141 taaatgatag tttcgggcat ggggtcggag atgaggtgct tgtttctttg gctcagatta
     1201 ttaagtcttg ttgccgtagt gaagatatta tttgtcggtt tggtggagaa gagtttgttg
     1261 tttttctgcc gaatacatct gtggtaacag cggagtgtgt tgcggagcgt attaggagtg
     1321 ttattgaacg tactgttttc cctaataact tgcgtgtcac cgtatctgct ggtgtggcaa
     1381 cacaagatga tcctcttggt gggatcgatt ttttgctgaa aaatgctgac gatgctcttt
     1441 atcaagctaa gagagagggg cgtaataaag tgattgtata tagcgctatt tgagtttttt
     1501 tgatgacgct ttacgcgcta gtgtgccgtt tttttttgtg gggagggggg ttcaagaagc
     1561 ggatcctctt gttatccctt cgatgtcgtc gtaatctcat gccctgcaaa caaaaaaacc
     1621 accgctacca acggtggttc gtttgccgga atcaagagct accaactctt tcttccgaag
     1681 gtaactggct ttcacggagc gcaggtacca aaactgtcct tccagtgtag ccgcagtcag
     1741 gccaccactt caagaactct gtagaacact ctactactcc gctccgtgaa tcctcttact
     1801 aatggctacc gccactggcg atttttcgtg tctgttccgg gttggactca agatgatagt
     1861 taccgggaca acgtggtagt cgggctgaac ggggggttcg tgcatacagt ccagcttgga
     1921 gcgaactgcc ttcccggaac cgagtgtcag gcgtggaatg agataaacgc ggccataaca
     1981 gcggattgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag
     2041 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
     2101 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttc gccgtggcct
     2161 tatcgcttcc ctgttaagct tcttcctggc atctcccagg aaatttccgc cccgcctgta
     2221 agccggtacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2281 ggaatatatc ttgtatcaca tattctgctg acgctccgct gctgcatttt tctcctgcca
     2341 catgaagcat ttcactgaca ctcgcatccg tgccaacata gtaagccagt atacactccg
     2401 ctagcgctac gtgactggtt cagggctgcg ccccgaaacc cgctaaaaac cactgacgcg
     2461 cctgcggctt gtccaacccc gcgccgaccg ggaaaggctt tcccgtccgt cacggggtta
     2521 tcaggaggcg attctggcag ataaacgcaa caagatgctc acgatgtggg tgacggaaga
     2581 cgaacaccgg cggttgcttg agcggtgcga cggaaaacaa ctggcggcgt ggatgcgcca
     2641 gacctgcctt gatgagaaac cgacacgcgc cggcaggctc ccctcgattt cgccggcgct
     2701 gctgcgtcag cttgctggca tgggcaataa cctgaaccag atcgcccgga aagtgaacgc
     2761 cggtggtgcc ggtcatgacc gggtgcagat cgtggcagcg ctgatggcga tcgatgccgg
     2821 tctagaacgg ttgcggcatg ccgtactgga aaaagggccg gacgatgatt gttaaattcc
     2881 atccacgggg tcgcggcggg ggagccggtc cggtggacta tcttctgggt aaagaccggc
     2941 agcgtgaagg tgccagcgtc ctgcagggga agccggaaga agtccgggag ctgattgatg
     3001 cgtcgccgta tgtgaaaaaa tatacctccg gggtgctgtc attcgcggag gcggacctgc
     3061 cgccgggtca gcgtgaaaaa ctgatggcca gctttgagcg ggttctgatg cccggactcg
     3121 ataaagatca gtacagcatt ctgtgggtgg aacacgcaga caagggtcgt cttgagctga
     3181 attttctgat ccccaatacc gaattactga cggggaaacg cctccagccg tattacgatc
     3241 gtgccgaccg gccgcgcatc gatgcgtggc agacagtggt caacggcagg ctggggctac
     3301 atgatccgaa cgacccggag aaccggcggg cgctggtcac gccgtcagga ctgccagaaa
     3361 cgaagcagga agccgcggag acgattacac ggggtttgct gatgttagcc tcatcaggag
     3421 agctgaaaac gcgtgatgac gtcacagggg cgctcacagc ggccggtttt gaggtggtca
     3481 gaaccacaaa aaacagtatc agcattgccg acccggacgg ggggcgaaat atccgactga
     3541 ggggagccat ttatgagcag tcttttaacg gcctcgaggg acttcgagga gaaatcgaaa
     3601 gcacagcagc agcttaccga agagaggctg aaagccgcat tcagcaagca cgagaacgtt
     3661 gtcaaatcgg aactgaacgc aagcgcgaag agaatcaacg acgccatcag cgcccacgaa
     3721 caggggatga acgcggcgat gcagtcgaac cggctgagcg tgctgcgaat ggtcgggcgg
     3781 acgtggctga ccatcaccat ggtgtcaggg ctgctgttcg ccagcctgag cggggtgctg
     3841 tggtaccagg ggaaccggat agcggcgaac ctggcggaaa tcaggcagca gagagatacg
     3901 ctgtcgaaac tgcagatgca gacgtggggc gtgacctatc tggagaaccg caacggacgg
     3961 tttctggtgc tgccggaggg gatgaaagcc gaaacgggcc ggacggtgga caacaagacg
     4021 cggaacgcgg tgaagctggt acgggagtag agcagaatga cggaactgga aaaacaattg
     4081 ctgagcgcgt tagagcagct acagcaggac tactcgagaa ggctggacga gtgggagagc
     4141 gccttcgagg aatggcggaa gatgtctggt cttatgcaac gggagaacgc ggcgctgagc
     4201 gagcgcgtgt cgggcttgag caggcaggtg cagagtttga gcgagcagct gcgccggtta
     4261 tcgacaggct gaatgagatt gatcaacagc gatacgggca aagaacggaa aaggaaccag
     4321 agaagacagc agatctggta ccggaacggc aacgcacatt atctcatggt gacagggagt
     4381 atggagactg ataaacgccc gtctcaggtg gcctgagcgt gacggggatc atggccattt
     4441 tctgcgattt cagcacacag agagtctttt ttaaccgaaa tcaccggcta tgcagtgatg
     4501 tgaataagtg atgcatatgc gtaaaataaa tcaggtgcgt ttctggtggt tttcggtgta
     4561 cttgccacca ccttttgccc gtcagccatc agaaacgccc tgagggcctc cggcgcggtg
     4621 cgggtaaggg atgttatggt aaatgcggca ttttttatta tatggagtgt atttttatga
     4681 gaattaaaaa gcctcacctt cgtccgatag tcataacctt gtccgttggt gggattttgt
//
