LOCUS       NZ_CP083774             2833 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain KSB1_9E isolate KSB1_9E plasmid
            pKSB1_9E_6, complete sequence.
ACCESSION   NZ_CP083774
VERSION     NZ_CP083774.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357330
            Assembly: GCF_020149895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Hawkey,J., Cottingham,H., Tokolyi,A., Wick,R., Judd,L.M.,
            Cerdeira,L., de Oliveira Garcia,D., Wyres,K.L. and Holt,K.E.
  TITLE     Linear plasmids in Klebsiella and other Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2021) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 135x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 15:43:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,490
            CDSs (total)                      :: 5,365
            Genes (coding)                    :: 5,219
            CDSs (with protein)               :: 5,219
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 55 of 146
            Pseudo Genes (incomplete)         :: 86 of 146
            Pseudo Genes (internal stop)      :: 27 of 146
            Pseudo Genes (multiple problems)  :: 19 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083774.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_9E"
                     /isolate="KSB1_9E"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKSB1_9E_6"
                     /geo_loc_name="Australia"
                     /collection_date="2013-09"
     gene            join(2351..2833,1..795)
                     /locus_tag="LB480_RS27475"
                     /old_locus_tag="LB480_27475"
     CDS             join(2351..2833,1..795)
                     /locus_tag="LB480_RS27475"
                     /old_locus_tag="LB480_27475"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_224518909.1"
                     /translation="MKSQFMHVELYSREEPAKKTINKSKNINHKSDVRTTNVDGVLSEM
                     KRDVGFTSHLEAIAAPEVLYGSINALERSIERYEAEYKTTDKNGKEKKLRKDACILLAG
                     VVSLDRADEEIWEEYKTKSIEYLKNKYGENLKCVVEHTDETNPHFHFYVVGNQQQDLNL
                     LHDGKLAVSKLAKEDKKKLHQTAYTEAMIKFQDDFYTKVSNKFGLSRTGEEPRERYKSR
                     PEYLRFKKRQETALNILSDIEEFEADIKEKARKTGYSKGAKQFQNKSWVIKLGEGIKLL
                     GKEHKAELDQKEKELEKIKKEKIEIETELNLKKEELSNAYIQRNEASRKNSVYIDKANA
                     LKKENKLLLPYKDQFDLKLQQEKEPLQAEVNNLTSENNNLKNENSELKKENIGFKEFID
                     GVKNHYGSKFEEWCKETFRTKTKHKY"
                     /name="plasmid recombination protein"
     gene            1482..2081
                     /locus_tag="LB480_RS27480"
                     /old_locus_tag="LB480_27480"
     CDS             1482..2081
                     /locus_tag="LB480_RS27480"
                     /old_locus_tag="LB480_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_063842281.1"
                     /translation="MAKAKVLIDDTEEAIIKNALDVDDMNEVNINVTRKKKTGFLGTYS
                     IVFHESVKTLSKMNLKPNAYKIVMYLFGIIEMGNILVNFSQKKIADDLGLQPSNVSRAF
                     NELFKKGVLIKDSEDGHVYLNSNLCTMGVPKNFDQKRMKSLQKSNIETEDFKNQINLFT
                     PTNTTKIKTNDKKVIKKKEKENVLEFPMQNDWVDIE"
                     /name="Crp/Fnr family transcriptional regulator"
ORIGIN
        1 aatttgctgc atgatggaaa gctagcagta tctaaattag caaaagaaga taagaaaaaa
       61 ttacatcaaa cagcttacac agaagctatg attaagtttc aagatgattt ttataccaaa
      121 gtatcaaata aatttggatt gagcagaacg ggcgaggagc cacgagaacg ctataaatcc
      181 cgccctgaat atttgaggtt taaaaaaaga caagaaacag cattaaatat attatctgat
      241 atagaagaat ttgaagctga tattaaagaa aaagcaagga aaaccggata tagcaaaggt
      301 gcaaagcaat ttcaaaataa aagctgggta attaagttag gtgaaggaat taaattactt
      361 ggaaaagagc ataaagcaga attagaccaa aaagaaaaag agttagaaaa aataaaaaaa
      421 gaaaaaatag aaattgaaac tgagcttaat ttgaaaaaag aagaattatc caatgcatat
      481 atccaaagga atgaagcgtc gaggaaaaat agtgtttata ttgacaaagc aaacgctcta
      541 aaaaaagaaa ataaattatt attaccatat aaagatcaat ttgatttgaa attacagcaa
      601 gaaaaggagc cattacaagc agaagtcaac aatttgactt ctgaaaataa caatttgaaa
      661 aatgagaatt cagaactaaa aaaagaaaat ataggtttca aagaatttat agatggcgtg
      721 aaaaatcatt atggatctaa atttgaagaa tggtgtaaag aaacattcag aacaaagaca
      781 aaacacaaat actaatctga aaatattttc atatttgccg cctatgtggt gtatgagaca
      841 ccactaaaag cggcctgaca gcgtttagct gtcatagctg ggatttatcc caaaaaaatg
      901 gttttgtacg cagttattaa ccttttaata tcagttactt aaatacataa aatgcaggtt
      961 gggaaaatgt ggtcagggag ctgaccgcat ggggtggggg gaccctgaac aagagtcact
     1021 tgttcaggtg gggggaacgg ggcggggttc cccctttggt atgtaactac atataaaaca
     1081 aaagaaatac tgttaaactt tgaaccgtga gaactgcaga acctctggtt ctgtggtttg
     1141 agcggttgtc caacgacccg gaggggaggc ggtaaggcag ttaaagttac ggtttcttct
     1201 ttcttatttt cttacttctt aagcaccctt taaaccctta tatatcaaca ctttaccaaa
     1261 aaaaaaatta tgaaaatcat aattttcaat tatgaaaatc ataatgcaaa attatgaaaa
     1321 tcataatcgg acttatgaaa atcataattt tgcatacagg ttcattgata tgatttgatg
     1381 tagaaacgat aactttctta tcgttttgat attgacattg cattcagatc ggttataaat
     1441 gcaacatagg ttattatttt tactacaaaa aggaatataa aatggcaaaa gcaaaagttt
     1501 taatagacga tacagaagaa gcaataataa aaaatgcatt agatgttgat gatatgaatg
     1561 aagtaaacat taacgtcacg agaaaaaaga aaaccggttt tctaggaact tattctatag
     1621 tttttcatga gtcagtaaaa acactgtcta aaatgaactt aaaacccaat gcttataaga
     1681 ttgtaatgta tctatttggc ataatagaaa tgggtaatat tttagttaac ttctcacaga
     1741 aaaagatagc tgatgattta ggcttgcagc cgtctaacgt ctcacgagca ttcaatgagc
     1801 tgtttaaaaa aggagtttta ataaaagata gcgaggacgg acacgtctac ttaaattcaa
     1861 atctatgcac gatgggtgtt ccgaaaaact ttgatcaaaa gagaatgaaa agtttacaaa
     1921 aatcaaatat tgaaactgaa gattttaaaa atcagatcaa tttattcact cctacaaata
     1981 caacaaaaat aaaaaccaat gataagaaag ttatcaagaa aaaagaaaaa gaaaacgttt
     2041 tagagtttcc tatgcaaaat gattgggttg atattgaata gtaaaactac cgcgcttcgc
     2101 ttgctgaata ccagaacatc gcaaatataa aatttgcaat gctctggtat tttcttttgg
     2161 ttttgactta aaccccgtag ggccgcatct atgaagccat attcgaagaa tatgatagcg
     2221 taatagatat agtcggttcc tataactatt gacagaaaaa ccaaatatgt tatttaaaat
     2281 ataagcaaaa caaaaaaggc tttcttcctg ttatgaagga aaaatattat atgtgaaaaa
     2341 ggaataaaat atgaaatcac aattcatgca tgtagaactc tacagccgag aagaaccggc
     2401 aaagaagact ataaataaaa gtaaaaatat aaatcataaa tctgatgtcc gtacaactaa
     2461 tgttgatggc gttttatcgg agatgaaacg agatgtaggc tttacatctc atttagaagc
     2521 catagctgca ccggaggtgc tttatgggtc tataaatgcc ttagaacgct ctatagagcg
     2581 ttatgaggcc gaatataaga ctactgataa aaacggcaag gagaagaagc tacgcaaaga
     2641 tgcatgcatc ttgctagctg gtgtagtttc tctagatcga gctgatgaag aaatttggga
     2701 agaatataag acaaaatcaa ttgaatattt gaaaaataaa tatggtgaaa atttaaaatg
     2761 tgttgttgag catacagacg aaacaaaccc acattttcat ttttatgtcg taggaaatca
     2821 gcaacaagat tta
//
