LOCUS       NZ_CP083773             4176 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain KSB1_9E isolate KSB1_9E plasmid
            pKSB1_9E_5, complete sequence.
ACCESSION   NZ_CP083773
VERSION     NZ_CP083773.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357330
            Assembly: GCF_020149895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Hawkey,J., Cottingham,H., Tokolyi,A., Wick,R., Judd,L.M.,
            Cerdeira,L., de Oliveira Garcia,D., Wyres,K.L. and Holt,K.E.
  TITLE     Linear plasmids in Klebsiella and other Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2021) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 135x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 15:43:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,490
            CDSs (total)                      :: 5,365
            Genes (coding)                    :: 5,219
            CDSs (with protein)               :: 5,219
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 55 of 146
            Pseudo Genes (incomplete)         :: 86 of 146
            Pseudo Genes (internal stop)      :: 27 of 146
            Pseudo Genes (multiple problems)  :: 19 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083773.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_9E"
                     /isolate="KSB1_9E"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKSB1_9E_5"
                     /geo_loc_name="Australia"
                     /collection_date="2013-09"
     gene            complement(join(3577..4176,1..825))
                     /locus_tag="LB480_RS27455"
                     /old_locus_tag="LB480_27455"
     CDS             complement(join(3577..4176,1..825))
                     /locus_tag="LB480_RS27455"
                     /old_locus_tag="LB480_27455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_224518906.1"
                     /translation="MIHKHIGGKKNQRSVKNSLDYLLRTNKPEEQKFVEVLSQTNRSDI
                     ENFNEYIKEKNFKNPFITGVLSFEEKDIDPELKTKIMNDFEDMMFSGIDPENRPPVIWI
                     QHKDKNRLELNYTTFNSLMNKKHFQCYYHLSDKKLFNSFCEKINYENNISSVLDVQEHK
                     ARNTLVNTLNNKIPEEKKVVVEKLQEEILSKIITEEINNREELISFIQSKNIIINRVRE
                     NAISIKFDKADKPISLKGDIYEQNRDYKTYRTESTIDHRADQQYVENQLRHHRENFDTE
                     LKKRHSRNRTRFKPTQSKDQKNVEFRIKKANDSKNVRSEISDNNNVPSRNEPDIDFFHN
                     NKTMEDVSNEQNFTNDKTKPREEEQAKFDLSREIEAVNTQQQFIRSRVEEAGRNQQQFR
                     KSLDRTKERSGVCRRSIYSIISNIRRYVRTLSIHTRQNERRQKIEHRQKIFLEKLKKQQ
                     TQNNQRSVVRSRFKPH"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(815..1165)
                     /gene="mobC"
                     /locus_tag="LB480_RS27460"
                     /old_locus_tag="LB480_27460"
     CDS             complement(815..1165)
                     /gene="mobC"
                     /locus_tag="LB480_RS27460"
                     /old_locus_tag="LB480_27460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_224518907.1"
                     /translation="MPDYKERYQRKNFISLCLSDEELSEVENIADRLNMKRAAAAREIL
                     VTNSKRLKSQIKKNDDAEILFLYSKISNNINQIAKKMNTNLDRFLSGNGEEFSLLIEEI
                     FEDLERLKNNDT"
                     /name="mobC"
     gene            complement(1165..1470)
                     /locus_tag="LB480_RS27465"
                     /old_locus_tag="LB480_27465"
     CDS             complement(1165..1470)
                     /locus_tag="LB480_RS27465"
                     /old_locus_tag="LB480_27465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224518908.1"
                     /translation="MSHEFLIQRNDNSNEQGYFQGYAEGVFYSAFNCEKFNNGVSGSNE
                     QETFTTAETKSALEKVLNSSEIANYPDPDRISDIKDFYFNIVLNAKNKDIFTVRFF"
                     /name="hypothetical protein"
     gene            complement(1745..3148)
                     /locus_tag="LB480_RS27470"
                     /old_locus_tag="LB480_27470"
     CDS             complement(1745..3148)
                     /locus_tag="LB480_RS27470"
                     /old_locus_tag="LB480_27470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023280855.1"
                     /translation="MNEKSIDEILAERQLKEFNESKFGQFFNNQKLKEQELTYLNNKAL
                     DIVNEEDLFEIMSLIPQPFITEENKNVDVLSLIYNNFLENTEIPLTLPIFSFLSFLSAF
                     CVKNEITYTIPLSDEKSPLDTWITILAPSGSAKTFSNSQIKKVIPVGIDGKKVIESNFV
                     KPNGPAKFIEELSNLPETKNGENQYGFWIEDEVSQMFKQIEQIGSPLSEIKEYLLKSYD
                     HEKLTRKSKKETIETKNIVLTLLFINTFESYANNISNESMTDGLMRRFNLIYTEKDSER
                     DFTDYSLYNLKKIRDETLQDKISELFNSIKPDQNFTFSDNCFKLYQKYFKIFWHRKYKT
                     ILPEKENFYRTYMMQSWKFAVFYHLILKREGTEIQGDCLVFGMKISLLFANSMKKFFDF
                     KLNKKLTPKKKEKIKVDFTKYIEYLNENTDITLRTFCRKFNLKKADSISILKSLECNNY
                     QHKLIEELE"
                     /name="hypothetical protein"
ORIGIN
        1 tctatggtgt ctgagttggt tttcgacata ctgctgatct gctctgtgat cgattgttga
       61 ttctgttcta taagttttgt aatctctgtt ctgttcatat atatctcctt ttaagctgat
      121 aggcttatca gccttatcaa attttattga tatagcattt tctctaaccc tgtttattat
      181 tatgttttta gactgaatga atgatattaa ttcttctctg ttgtttattt cttcggtgat
      241 tattttcgat aaaatctctt cttgtaattt ttcaacaact accttttttt cttctggtat
      301 tttattattt aaagtattaa caagggtatt tcttgcttta tgttcctgaa catctaaaac
      361 agaactgata ttattttcat agtttatttt ttcacaaaat gagttaaata attttttatc
      421 ggataaatga taataacatt gaaaatgttt tttattcatt agggaattga aagttgtata
      481 gtttaattca agtctgtttt tatctttgtg ttgtatccaa atgaccggag gtctattttc
      541 tggatctatt cctgaaaaca tcatatcttc aaaatcattc attatttttg tttttagttc
      601 tggatcgatg tctttttctt caaaagataa aacaccagtt atgaaagggt ttttgaagtt
      661 tttttctttt atgtattcgt tgaaattttc aatatcagat ctgtttgttt gagataaaac
      721 ctcaacaaat ttttgttctt ctggtttgtt ggttcttaat aaataatcaa gtgagttttt
      781 aacacttcgc tgatttttct ttcctccgat atgtttatgt atcattattt tttaacctct
      841 ctaaatcctc gaatatttct tcaatgagta atgaaaattc ttctccgttc cctgataaaa
      901 acctatccaa attagtattc attttttttg ctatctggtt tatgttgttt gatattttgg
      961 aatataaaaa caatatttca gcatcatcat ttttcttaat ttgtgatttt agtctctttg
     1021 agtttgtgac taatatttca cgagctgctg cggctcgttt catatttaac ctatcagcga
     1081 tattttcaac ttctgataat tcttcatcac tcaaacaaag agaaataaaa ttctttctct
     1141 gatatctctc tttgtaatct ggcattagaa aaatctcact gtaaaaatat ctttattttt
     1201 ggcatttaat acaatgttaa aataaaaatc tttaatatcg cttatacgat caggatctgg
     1261 ataatttgct atctcgctag aatttaagac tttttccagt gctgattttg tctctgctgt
     1321 ggtgaaagtt tcctgttcgt tagaaccaga aacaccatta ttaaattttt cacaattaaa
     1381 ggctgagtag aatacgcctt cggcatatcc ttggaaatag ccttgttcat tgctgttgtc
     1441 atttctctgg attaaaaact cgtgactcat attttgctcc ttggtttagg ttttgatttt
     1501 tggtttttag gggattgaag ggggaacccc tcacaagtaa aggaatacga agtattactc
     1561 taacttgcta tctattacat agataacaaa ttaaaacctt aagtcaaggg cgaaactgaa
     1621 tataatttaa ccttttataa aaccgtgaga aaataaaaaa gcccgtcagg gctttattat
     1681 ttttgagggg ttgaacaagc gaaaatatat ttgaatatgg cacaatttaa aattcgcttg
     1741 ttaattactc cagttcttca ataagtttgt gttgataatt attacattca agacttttca
     1801 aaattgatat tgaatcggct tttttcaggt tgaattttct gcaaaatgtt ctcaatgtaa
     1861 tatctgtatt ttcatttaaa tattcaatgt attttgtgaa atctactttt attttctctt
     1921 ttttctttgg tgttagtttt ttatttagtt tgaaatcaaa aaactttttc atactattgg
     1981 caaatagtag agatattttc ataccaaaca ctaaacaatc accctgaatt tctgttcctt
     2041 ctctttttaa aataaggtga taaaacactg cgaatttcca actttgcatc atgtaagttc
     2101 tataaaagtt ttctttctct ggtagaatgg ttttatattt tctatgccaa aaaatcttga
     2161 aatatttttg atataattta aaacaattgt ctgaaaatgt gaaattttga tctggtttta
     2221 ttgagttgaa taattcagat attttgtcct gtaatgtctc atctcttatt tttttcaaat
     2281 tatataatga gtaatctgtg aaatctcttt cactgtcttt ttctgtataa atgagattaa
     2341 atcttctcat taaaccatca gtcattgatt catttgaaat attattcgca taactttcaa
     2401 atgtatttat aaataacagg gttaaaacta tatttttagt ttctattgtt tcttttttgc
     2461 ttttccttgt taatttttca tggtcatatg actttaacaa atattcttta atttcagata
     2521 atggagatcc gatttgttct atctgtttaa acatttgtga aacttcatct tcaatccaaa
     2581 aaccatattg attttcgccg tttttggttt cgggaagatt tgaaagttct tcgataaatt
     2641 tcgcagggcc attaggtttt acaaaatttg actcaataac tttttttccg tcaatgccta
     2701 ctggtataac cttttttatt tgtgaatttg aaaaagtttt cgcacttcca gaaggtgcca
     2761 aaattgtgat ccaagtatca agcggcgatt tttcgtcgct taatggaatt gtatatgtga
     2821 tttcattttt aacacaaaaa gcacttaaaa aacttaaaaa agaaaaaata ggaagtgtta
     2881 atggtatttc tgtattttct aaaaagttat tatatataag gcttaaaaca tcaacatttt
     2941 tattttcttc tgtaataaat ggttgaggta ttaatgacat tatttcgaat aaatcttctt
     3001 cattcactat gtctaatgct ttattgttaa ggtatgttag ttcttgctct tttagttttt
     3061 gattattgaa aaactgacca aatttcgatt cgttaaattc ttttagttgt ctttcagcta
     3121 atatttcatc aattgatttt tcattcataa ttaatttctc ctattttggt tatattacat
     3181 atataacata tgaaaaaaca ttgtcaacgg tttttgcgaa aaacctatga cacgttttaa
     3241 accttgattt atgacactat gacactatga cactacggac aaagccttga tacataagga
     3301 tttaaccact ttttagggtt atgacaaaac ctatgacaaa agttatgaca cttgtcttaa
     3361 aatcacaatt gagaaaaaaa gctgtttttt atgtcataag aaaaggaaaa atattcagac
     3421 aggttcttgt caaaaactcc gacaaagtta tgacagaaaa accgccctga ccgggcaggc
     3481 caacccctca caagcaaaaa ccagcctctg gctggttctc acttgttcac ggttaaggtt
     3541 ttatcgtagt gggttagaag agtgttcaga acggttctaa tgcggtttaa atcgacttct
     3601 gacgacactt ctttgattgt tttgtgtttg ctgttttttg agcttttcaa ggaagatttt
     3661 ttgtctgtgt tcgatttttt gtcttctctc gttctgtctt gtatggattg aaagcgttct
     3721 gacatatctt ctaatattag aaataattga gtatatagat cttctacata ctccagatcg
     3781 ttctttagtt ctatcaagcg actttctgaa ttgctgttga tttcttccag cttcttcaac
     3841 tcttgaacga ataaattgtt gttgtgtatt aactgcttca atttctcttg atagatcaaa
     3901 ttttgcttgt tcttcttctc ttggttttgt tttatcattt gtgaaatttt gctcattgct
     3961 tacatcctcc atagtcttat tattatgaaa aaaatcaata tcaggttcat tcctagaagg
     4021 aacattatta ttgtctgata tttcagatct aacattttta ctgtcatttg cttttttaat
     4081 tctgaactca acatttttct ggtctttgct ctgtgttggc ttaaaacgag ttctatttct
     4141 gctgtgtctt tttttaagct ctgtgtcaaa attttc
//
