LOCUS       NZ_CP083771             6352 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain KSB1_9E isolate KSB1_9E plasmid
            pKSB1_9E_3, complete sequence.
ACCESSION   NZ_CP083771
VERSION     NZ_CP083771.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357330
            Assembly: GCF_020149895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Hawkey,J., Cottingham,H., Tokolyi,A., Wick,R., Judd,L.M.,
            Cerdeira,L., de Oliveira Garcia,D., Wyres,K.L. and Holt,K.E.
  TITLE     Linear plasmids in Klebsiella and other Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2021) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 135x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 15:43:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,490
            CDSs (total)                      :: 5,365
            Genes (coding)                    :: 5,219
            CDSs (with protein)               :: 5,219
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 55 of 146
            Pseudo Genes (incomplete)         :: 86 of 146
            Pseudo Genes (internal stop)      :: 27 of 146
            Pseudo Genes (multiple problems)  :: 19 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083771.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_9E"
                     /isolate="KSB1_9E"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKSB1_9E_3"
                     /geo_loc_name="Australia"
                     /collection_date="2013-09"
     gene            complement(837..1070)
                     /locus_tag="LB480_RS27380"
                     /old_locus_tag="LB480_27380"
     CDS             complement(837..1070)
                     /locus_tag="LB480_RS27380"
                     /old_locus_tag="LB480_27380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_040088065.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1077..1607)
                     /locus_tag="LB480_RS27385"
                     /old_locus_tag="LB480_27385"
     CDS             complement(1077..1607)
                     /locus_tag="LB480_RS27385"
                     /old_locus_tag="LB480_27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_040088066.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTAALKELLERYQKTTVSTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1645..2076)
                     /locus_tag="LB480_RS27535"
                     /pseudo=""
     CDS             complement(<1645..2076)
                     /locus_tag="LB480_RS27535"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2285..2608)
                     /locus_tag="LB480_RS27395"
                     /old_locus_tag="LB480_27395"
     CDS             complement(2285..2608)
                     /locus_tag="LB480_RS27395"
                     /old_locus_tag="LB480_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_059287943.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLAEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELCSLRQVVREQGAR
                     NDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2673..2864
                     /locus_tag="LB480_RS27400"
                     /old_locus_tag="LB480_27400"
     CDS             2673..2864
                     /locus_tag="LB480_RS27400"
                     /old_locus_tag="LB480_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3868..4536)
                     /locus_tag="LB480_RS27405"
                     /old_locus_tag="LB480_27405"
     CDS             complement(3868..4536)
                     /locus_tag="LB480_RS27405"
                     /old_locus_tag="LB480_27405"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein phosphatase"
                     /protein_id="WP_223195384.1"
                     /translation="MGYIQMIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCS
                     EWNMKILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYN
                     KSGVLLTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFD
                     LNDENKEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS
                     "
                     /name="PP2C family serine/threonine-protein phosphatase"
     gene            complement(4518..5384)
                     /locus_tag="LB480_RS27410"
                     /old_locus_tag="LB480_27410"
     CDS             complement(4518..5384)
                     /locus_tag="LB480_RS27410"
                     /old_locus_tag="LB480_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="WP_000409995.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            complement(5368..6324)
                     /locus_tag="LB480_RS27415"
                     /old_locus_tag="LB480_27415"
     CDS             complement(5368..6324)
                     /locus_tag="LB480_RS27415"
                     /old_locus_tag="LB480_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000280763.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     Typing          complement(2950..3032)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="3.99e-36"
     Typing          3048..3605
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.831"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(734..2295)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.585"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2292..2641)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.744"
                     /coverage="99.0"
                     /evalue="6.74e-62"
ORIGIN
        1 agaatatacg tcctaaggac gtatattcta ttagagattg attgtcaaga aaaaaataat
       61 aacaaacaag catttgccat ttttatattt ttgatatcct ataaaataaa tcctaaggat
      121 ttattttata ggataataag taggaagtca agaaaaagtt tacttatatc accgttttca
      181 taacacctca tgcgatcaat tcagcggcgg ttatagttca aatccatgat taggacatct
      241 gttgctcata aacgatgagc gtaaaagatc atttttcagc gtcatttacc ataacgtcgc
      301 attgtacgca ccgccaaaac gcgttcggtg cgatcacggc agcagacagg taaaaatggc
      361 aacaaaccac ccgaaaaact gccgcgatcg cacccgataa attttaacca catgcatagc
      421 tatgcagcca tgtgaatcac gcaggaattg ccagccagag actgctgaaa cgtatttttt
      481 tcatactttc ggaaaggcag aagacggaac cggagcggaa aaacagaaat tcaggggaat
      541 gagcgcgatc tggcaataac ggcaaaacac agcaacaaag acacaccaga atcgcgcccg
      601 tatgcgtttt aacgcgttcc tgtgttttca gcaagctctg actgaagcgc gttcccggtg
      661 cccccgaacc aaccattatc ccaaaaaatg caccggaaac gcgctgccaa cattttagcc
      721 tgtctctggt taccatccca gcgaagggcc atccagtgtc cgttcctgaa tttccggctg
      781 gcgctcccgt tccagagata actcatgttc gcgttcctgc atcagccgtt catcccttat
      841 ctcctgttct cgctgtataa ctggctcaag cgttctgtcc gctcgctcaa gtgctgcacc
      901 tgctgactca actgcatgac ccgcccgttc agcatcgcgt tgtcccgttg cgtaagcgaa
      961 aacattttct gcaattccac gaaggcgctc tcccattcgt tcagccgctg catatagtcc
     1021 tgttgcagtt gctctaatgc gttcagcaaa tgtctttcca gctccgtcat gctctgtcac
     1081 tcctgcacca gtctcactgc gttcagtttg ccgttgtcct tcgtccagtt cgtttcggct
     1141 ttcatcccct tcggcaacac cagaaaacgt ccgttgctgt cctcgtgata acgcacgccc
     1201 catgtcttcg cattcagctt ctccagactc tctttctgct cgcggagaat attcatattc
     1261 gcgtttatct tcatgccctg gtaccacagc agactcccgg tcgtggcaaa catcagtact
     1321 gagacgataa tcagccacag ccagcgtttc acgctcattt tcagcacggt ttgccagtgt
     1381 gtgtccatcg tgctgacggt cgttttctga tacctctcca gaagttcttt cagggctgct
     1441 gtatggtcgt tgatagcgtt cctgattaaa gattcgtttg aggtcagttc cctgtttaca
     1501 gattcttcga gcttcctgaa ctcgtcgttc agcattttct ctgtagattc ggctctctct
     1561 ttcagctttt tctcgaactc cgcgcccgtc tgcaaaagat tgctcataaa atgctccttt
     1621 cagcctgata ttccttccgc cgttcgggtc tgcaatgctg atactgcttc gcgtcaccct
     1681 gacaacctca actcccacat ctgtgagtgc ctgaatcaca tcctgacggt cttttatctc
     1741 tccggcacgg taaagcacat caagcccccg tgtaacgctc tcagcaatcg cctgtttcgt
     1801 ttccggcagg ttatcaggga gtgtcagcgt cctgcggttc tccggggcat tcgggtcatg
     1861 cagcccgtaa tggtgattca tcagcgtctg ccaggcatca attctcggcc tgtctgcgcg
     1921 gtcatagtac ggctggaggc gttttccgct ctgtagctcc atgttcggaa tgacaaaatt
     1981 cagctcaagc cgtcccttgt cctggtgctc cacccacagg atgctgtact gatttttttc
     2041 gagaccgggc atcagtacac gctcaaagct cgccatcact ttttcacgcc ctcccggcgg
     2101 cagctccttc tccgcgaacg acagaacacc tgacgtgtat ttcttcgcaa atagcgtggc
     2161 atcgatgagt tcccggactt cttccggatt accctgaagc accgttgccc cttcccggtt
     2221 acgctccctt cccagcaggt aatcaaccgg accactgcca ccacctttcc ccctggcatg
     2281 aaatttaaca atcattccgc gctccctgtt ccctgacgac ctgccgtaag ctgcacaact
     2341 ctctctcgat ggccatcagt gcggccacca cctgaacccg gtcaccggaa gaccactgcc
     2401 cgctgttcac cttccttgct gtctgattca ggttattccc gatggcagcc agctgacgca
     2461 gtaacggcgg cgccagtgtc ggcagcttcc cggaacgggc aaccggctca gccaggcaga
     2521 cccgccgcat ccataccgcc agttgtttac cctcacagcg ttccagtaac cgggcatgtt
     2581 catcatcagt aacccgtatc gtgagcatcc tctcgcgttt catcggtatc attatcccca
     2641 tgaacagtaa tcccccttac acggaggcat cagtgactaa acaggaaaaa accgccctta
     2701 acatggcccg ctttatcaga agccagacat taacgctgct ggagaagctc aacgaactgg
     2761 acgcggatga acaggctgat atctgtgaat cgcttcacga ccacgccgat gagctttacc
     2821 gcagctgcct cgcacgtttc ggggatgacg gtgaaaacct ctgacacatg cagctcccgg
     2881 agacggtcac agcttgcctg tgagcggatg ccgggagcag acaagcccgt cagggcgcgt
     2941 cagcgggttt tagcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     3001 tgtatactgg cttaactatg cggcatcagt gcgaattgta tgaaaagtgc cccatgccgg
     3061 gtgtgaaatt ccgcacagat gcgtaaggag aaaatgcgcg tccaggcgct tttccgcttc
     3121 ctcgctcact gactcgctac gctcggtcgt tcggctgcgg cgagcggtac tgacttacac
     3181 aaaaacggca gcacggttat ccacagaatc aggggataag gccggaaaga acatgtgagc
     3241 aaaagaccaa gaacaggaag aaggccgcgc cgcaggcgtt tttccatagg ctccgccccc
     3301 ctgacgagca tcacagaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactta
     3361 aagataccag acgtttcccc ctggtggctc cctcgtgcgc tctcctgttc cgaccctgcc
     3421 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3481 acgctgttgg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3541 accccccgtt cagcccgact gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3601 ggtaagacac gccttaacgc cactggcagc agccactggt aactgattta gaggagttag
     3661 tcttgaagtc atgcgccagt taaggctaaa ctgaaagaac aggttttggt gactgcgctc
     3721 ctccaagcca gttaccttgg ttcttcgagc cgcaaactct ttgaacctaa gagaaaaccg
     3781 ccccgcaaag gcggtttttt tcttttacag aaaagagatt atctatatga cacgaagatc
     3841 tcaatagctt cttcaaaaaa cacgaaatca agaagggaat ctaacgtagc aaaccgtatg
     3901 attctctgcg ttacccggga tatttttagt aaatttagcc aagttagatt ctgggttatc
     3961 tttcatgagg tcaataacag taccatcatt aaaatgctcc caaaacccat cagtacataa
     4021 aataacctct ttgttttcat catttaaatc aaaaactgaa tgattgaaaa ttatctcatc
     4081 agagcagaaa tcaacaaatg atgttagata atttctgtct gggcaataac aaacgaggtg
     4141 aggtattttt ttattatcga ttccattttc ctttaatctt ttccaggcaa ccgagttatc
     4201 ctctgttaga aggactccac ttttgttata tacacggcaa tctcctgcgt tatatatatg
     4261 gaggtgcgta ttatttttaa aaaggaaaca tactgcagcc ttaacaggaa ctgacttgca
     4321 tggttcaata atatctttat tcagatcacc agaaaacacg cacttatcta acctaccaga
     4381 gagaattttc atattccact cagagcagga atacccatcc ataataacca ttccaaacac
     4441 atcattttga caatttatcg tagataataa gtctaagttt tcacatctgt taccaatatc
     4501 actaaaactg aacgctatca tttgaatata tcccacggaa gactttcata gaaacaggca
     4561 actttttcat aagcatcttc aaaaagaggt aatgatttaa cggtggatga taaatcctga
     4621 tatcgctgac tggaacgctc gataatttct acatccctta tacctttaac gtttaagaga
     4681 ttatccaatt gtttaccttc actctttttt aaaagagact ccagtatttt atacttatct
     4741 tgccgagaaa actcatattt acctaaaaga taattgatat catatatatc ttgctctcga
     4801 tttctctctc taactttttg ttgcaaaacg gatcgatatt tttcagccat caaatcagtt
     4861 aagctgtagg tctttacgct atcattgtca tcatcaccat ctaatgataa aaagtcaaaa
     4921 tcatacgttt tttcgttaaa gctaaaatcg atatttatca ctgaaggtga ttgattagat
     4981 tcaagccttt tcattgcaga cgcattagaa cgatcgcaaa aggctatatt tatctgtaca
     5041 gttggaaatg ttccttgtgc attaggttga attttatgag attgaacttt acatttaata
     5101 ccatatccca attcagcctc agcaacatcc attgagtcat taaacctatt gaaaaaatcc
     5161 cccaagtcaa tttcagaaag ttttttttcg gtagaaaaat caatatcctt tgtgtatcta
     5221 tcactaccat gcttaacacc aagaagagtc cctcccttca taaccatcat ttcattcaaa
     5281 ctctcctcta tagaaataga tttaagtatt atatgtacag cctggcgcat caacctgtac
     5341 ttcccaccag actcttcgat ccaattatta atttcttttt ccataagact taaccatatc
     5401 aacattaata gaaataaacc attcttcatc aaagtaaggt gagaatggac tctttggaga
     5461 tagcactttc gaactacctc tgtatttacc cgcctcttgt ttccattcgc ttattttttt
     5521 attattaacc ccaataattc tatccaaaat gaatccaacc cttaccttgt caatatgacg
     5581 gccaagctta tcgacttcct ttataatcag aggtgcatat tttttaccgt gctcaataaa
     5641 tgattccaat acatgctcct caccaccaca gtaatcaggc cgcctggtca tatccataaa
     5701 ggtcttaccg atagatgata ccctgacagg ggaattacga gccttcattg gctcaacata
     5761 atttgtttct tgtgtaacga taatttttat tccattgacc tcagaatttc ttggaaatct
     5821 aggcatgaat tgtttggctt cgtctatatc aggattatcc ttttgaaaat catgatagaa
     5881 cttattcttc cactgttcgg aacgacatgt cgtaaagtgg attatcttgg gtattttatc
     5941 tgtgatgtta tgccaatcca tagcggataa ataggataag tatccatatg gatacgtaga
     6001 gcacactata tcattttccg gagaaccatc gttaccttta acaaaaaaga cctcatagaa
     6061 accgccaatt aaatgggggt aagcatgatg cttatcgtta ctttcaactc ttgataaaac
     6121 ccctgacttt aataatttta atatattttt ttcaaattca ttaggtcctg ctttgttctt
     6181 tctaatttga tatatcctct caccattata ttcttttttt aaataaaaaa ggtaaatctg
     6241 caaagagatt tcttctttgg ttatgactgg ttttttatat tcattcaaca accaagacgt
     6301 tagcgcttct tcgataagat acatattcaa ccttttcggt ttagtctcta at
//
