LOCUS       NZ_CP083730             2473 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Staphylococcus aureus strain MR254 plasmid pMR254-2, complete
            sequence.
ACCESSION   NZ_CP083730
VERSION     NZ_CP083730.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21341543
            Assembly: GCF_020150915.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Wang,X., Yu,F. and Zhou,Y.
  TITLE     MRSA harboring novel SCCmec structure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Wang,X., Yu,F. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) Department of Clinical Laboratory Medicine,
            Shanghai Pulmonary Hospital, Yangpu District, Shanghai 200082, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-SEP-2021
            Assembly Method                   :: Flye v. V2.5; CANU v. V1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 01:43:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,888
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,734
            CDSs (with protein)               :: 2,734
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 38 of 72
            Pseudo Genes (incomplete)         :: 41 of 72
            Pseudo Genes (internal stop)      :: 13 of 72
            Pseudo Genes (multiple problems)  :: 18 of 72
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083730.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="MR254"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pMR254-2"
                     /geo_loc_name="China"
                     /collection_date="2021-08-01"
     gene            join(2316..2473,1..577)
                     /gene="erm(C)"
                     /locus_tag="K9B11_RS14405"
                     /old_locus_tag="K9B11_14405"
     CDS             join(2316..2473,1..577)
                     /gene="erm(C)"
                     /locus_tag="K9B11_RS14405"
                     /old_locus_tag="K9B11_14405"
                     /EC_number="2.1.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /protein_id="WP_001003263.1"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT
                     LELVQRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNIPY
                     NISTDIIRKIVFDSIADEIYLIVEYGFAKRLLNTKRSLALFLMAEVDISILSMVPREYF
                     HPKPKVNSSLIRLNRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID
                     DLNNISFEQFLSLFNSYKLFNK"
                     /name="erm(C)"
     gene            1025..1501
                     /locus_tag="K9B11_RS14410"
                     /old_locus_tag="K9B11_14410"
     CDS             1025..1501
                     /locus_tag="K9B11_RS14410"
                     /old_locus_tag="K9B11_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000664727.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            2196..2255
                     /gene="ermCL"
                     /locus_tag="K9B11_RS14415"
                     /old_locus_tag="K9B11_14415"
     CDS             2196..2255
                     /gene="ermCL"
                     /locus_tag="K9B11_RS14415"
                     /old_locus_tag="K9B11_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WP_002497446.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     ARG             2317..2471
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="100.0"
                     /coverage="21.31"
     ARG             3..577
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.48"
                     /coverage="78.28"
     Typing          1026..1511
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.387"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttcgtaact gccattgaaa tagaccataa attatgcaaa actacagaaa ataaacttgt
       61 tgatcacgat aatttccaag ttttaaacaa ggatatattg cagtttaaat ttcctaaaaa
      121 ccaatcctat aaaatatttg gtaatatacc ttataacata agtacggata taatacgcaa
      181 aattgttttt gatagtatag ctgatgagat ttatttaatc gtggaatacg ggtttgctaa
      241 aagattatta aatacaaaac gctcattggc attattttta atggcagaag ttgatatttc
      301 tatattaagt atggttccaa gagaatattt tcatcctaaa cctaaagtga atagctcact
      361 tatcagatta aatagaaaaa aatcaagaat atcacacaaa gataaacaga agtataatta
      421 tttcgttatg aaatgggtta acaaagaata caagaaaata tttacaaaaa atcaatttaa
      481 caattcctta aaacatgcag gaattgacga tttaaacaat attagctttg aacaattctt
      541 atctcttttc aatagctata aattatttaa taagtaagtt aagggatgca gtttatgcat
      601 cccttaactt gtttttcgtg tacctatttt ttgtgaatcg attatgtctt ttgcgcattc
      661 acttcttttc tatataaata tgagcgaagc gaataagcgt cggaaaagca gcaaaaagtt
      721 tcctttttgc tgttggagca tgggggttca gggggtgcag tatctgacgt caatgccgag
      781 cgaaagcgag ccgaagggta gcatttacgt tagataaccc cctgatatgc tccgacgctt
      841 tatatagaaa agaagattca actaggtaaa atcttaatat aggttgagat gataaggttt
      901 ataaggaatt tgtttgttct aatttttcac tcattttgtt ctaatttctt ttaacaaatg
      961 ttcttttttt tttagaacag ttatgatata gttagaatag tttaaaataa ggagtgagaa
     1021 aaagatgaaa gaaagatatg gaacagtcta taaaggctct cagagactca tagacgaaga
     1081 aagtggagaa gtcatagagg tagacaagtt ataccgtaaa caaacgtctg gtaacttcgt
     1141 aaaggcatat atagtgcaat taataagtat gttagatatg attggcggaa aaaaacttaa
     1201 aatcgttaac tatatcctag ataatgtcca cttaagtaac aatacaatga tagctacaac
     1261 aagagaaata gcaaaagcta caggaacaag tctacaaaca gtaataacaa cacttaaaat
     1321 cttagaagaa ggaaatatta taaaaagaaa aactggagta ttaatgttaa accctgaact
     1381 actaatgaga ggcgacgacc aaaaacaaaa atacctctta ctcgaatttg ggaactttga
     1441 gcaagaggca aatgaaaaac aagaaaatgc attatctgat tattattctt tcaaggacta
     1501 gtataacata aaatcgtcta caaatagaca aaaaacctgc acgcttaatg tagatcaaaa
     1561 gcttaacgca aatgaaatag attgacctcc caataacacc acgtagttat tgggaggtca
     1621 atctatgaaa tgcgattaag ctttttctaa ttcacataag cgtgcaggtt taaagtacat
     1681 aaaaaatata atgaaaaaaa gcatcattat actaacgtta taccaacatt atactctcat
     1741 tatactaatt gcttattcca atttcctatt ggttggaacc aacaggcgtt agtgtgttgt
     1801 tgagttggta ctttcatggg attaatccca tgaaaccccc aaccaactcg ccaaagcttt
     1861 ggctaacaca cacgccattc caaccaatag ttttctcggc ataaagccat gctctgacgc
     1921 ttaaatgcac taatgcctta aaaaaacatt aaagtctaac acactagact tatttacttc
     1981 gtaattaagt cgttaaaccg tgtgctctac gaccaaaagt ataaaacctt taagaacttt
     2041 cttttttctt gtaaaaaaag aaactagata aatctctcat atcttttatt caataatcgc
     2101 atcagattgc agtataaatt taacgatcac tcatcatgtt catatttatc agagctcgtg
     2161 ctataattat actaatttta taaggaggaa aaaatatggg catttttagt atttttgtaa
     2221 tcagcacagt tcattatcaa ccaaacaaaa aataagtggt tataatgaat cgttaataag
     2281 caaaattcat tataaccaaa ttaaagaggg ttataatgaa cgagaaaaat ataaaacaca
     2341 gtcaaaactt tattacttca aaacataata tagataaaat aatgacaaat ataagattaa
     2401 atgaacatga taatatcttt gaaatcggct caggaaaagg gcattttacc cttgaattag
     2461 tacagaggtg taa
//
