LOCUS       NZ_CP083713             4461 bp    DNA     circular CON 09-AUG-2023
DEFINITION  Enterobacter cloacae complex sp. ECL352 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP083713
VERSION     NZ_CP083713.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21399027
            Assembly: GCF_020149545.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae complex sp. ECL352
  ORGANISM  Enterobacter cloacae complex sp. ECL352
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Zhang,X.
  TITLE     Molecular characterisation of NDM-1-Producing Carbapenem-Resistant
            E. cloacae complex From a Three Tertiary Hospitals in
            Chongqing,China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2021) Department of Microbiology, Yongchuan
            Hospital of Chongqing Medical University, Xuanhua Road, Yongchuan
            District, Chongqing 402106, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 550.0x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/07/2023 02:05:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,906
            CDSs (total)                      :: 4,788
            Genes (coding)                    :: 4,679
            CDSs (with protein)               :: 4,679
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 28 of 109
            Pseudo Genes (incomplete)         :: 80 of 109
            Pseudo Genes (internal stop)      :: 21 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            Pseudo Genes (short protein)      :: 1 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083713.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterobacter cloacae complex sp. ECL352"
                     /mol_type="genomic DNA"
                     /strain="ECL352"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:2876028"
                     /plasmid="unnamed4"
                     /geo_loc_name="China:Yongchuan District, Chongqing"
                     /collection_date="2020-09-24"
     gene            1..486
                     /locus_tag="LBF07_RS24555"
                     /old_locus_tag="LBF07_24555"
     CDS             1..486
                     /locus_tag="LBF07_RS24555"
                     /old_locus_tag="LBF07_24555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023139312.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLTASGASILWWQGQQIL
                     DNYTTIREQKRTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            490..705
                     /locus_tag="LBF07_RS24560"
                     /old_locus_tag="LBF07_24560"
     CDS             490..705
                     /locus_tag="LBF07_RS24560"
                     /old_locus_tag="LBF07_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_058671277.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            876..1352
                     /locus_tag="LBF07_RS24565"
                     /old_locus_tag="LBF07_24565"
     CDS             876..1352
                     /locus_tag="LBF07_RS24565"
                     /old_locus_tag="LBF07_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224435168.1"
                     /translation="MSFTVASAENITYTKPSAGRDLYLTCKGYQVKGASVINVEKPSYL
                     EGTGEQVEFAIISLGDASDGLKVFFDSWGFSSFGLIESHVMHEHWSLVHVGGRVSVKGE
                     KIDYSFVDNQPQEGGSYKPKTGVFLYGCINSDKRPSWVEKIYTRTGVFRGGIED"
                     /name="hypothetical protein"
     gene            complement(1535..1864)
                     /locus_tag="LBF07_RS24570"
                     /old_locus_tag="LBF07_24570"
     CDS             complement(1535..1864)
                     /locus_tag="LBF07_RS24570"
                     /old_locus_tag="LBF07_24570"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052909321.1"
                     /translation="MKILPAEPGLYFFASFESGAHVYKVVAWSENENGGLDPQCVLTAL
                     YDKSSSSQQWELHSTSASAYQHSNDGRPFMYFITYFTSNGGIPELYNAINTLAERWGYP
                     LIDLP"
                     /name="hypothetical protein"
     gene            complement(1948..2379)
                     /locus_tag="LBF07_RS24575"
                     /old_locus_tag="LBF07_24575"
     CDS             complement(1948..2379)
                     /locus_tag="LBF07_RS24575"
                     /old_locus_tag="LBF07_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_053274236.1"
                     /translation="MNIPTHLKHKPIIAVENYDRIDGPYADDTDAMGLSVGIAQWNTPG
                     YTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICIAMDYSKHGRLSSSDDFPVSRSG
                     GNPELSRHIDLMKNELKENHEYLDESLKRLAAELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(2709..2822)
                     /locus_tag="LBF07_RS24580"
                     /old_locus_tag="LBF07_24580"
     CDS             complement(2709..2822)
                     /locus_tag="LBF07_RS24580"
                     /old_locus_tag="LBF07_24580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            3461..3784
                     /locus_tag="LBF07_RS24585"
                     /old_locus_tag="LBF07_24585"
     CDS             3461..3784
                     /locus_tag="LBF07_RS24585"
                     /old_locus_tag="LBF07_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001347450.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3966..4211)
                     /locus_tag="LBF07_RS24590"
                     /old_locus_tag="LBF07_24590"
     CDS             complement(3966..4211)
                     /locus_tag="LBF07_RS24590"
                     /old_locus_tag="LBF07_24590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224435167.1"
                     /translation="MQPQPAVHDGLPGINARTVGAVVIRLETLAGQQLRVRDQEVQLQP
                     PLILVLNPQHAVLVFIESGHQNTFKARHQLFALPGR"
                     /name="hypothetical protein"
     Typing          3175..3357
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.737"
                     /coverage="86.0"
                     /evalue="2.11e-43"
     Typing          complement(2710..3211)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.179"
                     /coverage="96.0"
                     /evalue="1.29e-158"
     Typing          4..797
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.547"
                     /coverage="99.0"
                     /evalue="8.9e-144"
     Typing          3775..4460
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.817"
                     /coverage="98.0"
                     /evalue="4.71e-147"
ORIGIN
        1 atgaacagtc ttttaacgct ggcaaaggac ttagagcaga aatcgaaagc gcagcagcag
       61 agtaccggcg agatgctgaa agccgcattc agcgagcacg agaagtctgt cagagcggaa
      121 ctgagcgaaa gcgagaagag aatcagcgcc gccatcctcg accacgaccg gaagctgtcc
      181 tcagccatga gccagcgcac gaaaggcatg gtgcgtatgg tcagccagac gtggctgacc
      241 atcgtcctgg tgtctgcact gctgacagca tcgggcgcaa gcattctgtg gtggcagggg
      301 cagcagatac tcgacaatta cacgaccatc cgggagcaga agcgcacgca ggccatgctg
      361 tccgagagga acagcggcgt gcagctctcg acctgcggag agcagagacg ccgctgcgtg
      421 agggtgaacc cggaagcggg acggttcgga gaggattcga gctggatgat actggcgggg
      481 aaatagcaca tgacggagct ggaaaaacag ttgctgagcg cattagagca gctacagcag
      541 gactactcga aaaggctgga cgagtgggag aacgccttcg cggaatggcg gacgatgtct
      601 ggtcttatgc aacgggagaa cgcggcgctg agcgagcgcg tcacgggctt gagcaggcag
      661 gtgcagagtt tgagcgatca gctgcgccgg ttgtcgaaag gctgaactcg attgaagctc
      721 acaggcagca ggagcgggcg gcgcagcatc agaaaacgct ggagctggaa cgatcgcagc
      781 ggcagaaagg ttttgagctt ggaatgtagt taatcaatga aaagggatgc gttatgaagt
      841 gtttctgggc ttgctcagtc tcagtagttt tggttatgtc atttacagtc gcttctgcgg
      901 agaacattac atacacaaaa ccgtcagccg ggagggactt atatcttacc tgtaaaggtt
      961 atcaggtgaa gggggcgtct gtaattaacg tcgagaaacc ttcttacctt gaaggtacag
     1021 gtgagcaggt tgagtttgca atcatttctc tcggtgatgc atctgatgga ctcaaggttt
     1081 tttttgattc atggggtttt tcttcttttg gattgattga aagtcatgtg atgcatgagc
     1141 actggtcttt agttcatgtt ggggggcgtg tttcagtcaa gggtgaaaag attgattatt
     1201 catttgtaga taaccagcca caggagggtg ggagctataa acccaaaaca ggtgtgttcc
     1261 tgtatggctg cattaactct gataaaaggc catcttgggt cgaaaaaatc tacacaagaa
     1321 caggcgtgtt tcgagggggc atagaagact aaaatactgc gtacagcatg aaaattcatt
     1381 gaacagagtg ttagttcatt ttcgtgcatg gctatgcata cacgcaaaac ttatcagcgc
     1441 gattccgggc ggttttcggt gagaagacgg gctcttttgt ggctcaggac ggaaaaacgc
     1501 cctgtgcgcg tctgagcggt gcgcgcaaga gatgttatgg taaatcaatt aatgggtatc
     1561 cccatcgttc ggctagtgtg tttattgcgt tataaagttc aggtatcccg ccattgctgg
     1621 tgaagtaggt gataaaatac ataaatggtc tgccgtcatt gctgtgctga tatgcagatg
     1681 ctgaagttga gtgaagttcc cattgctggc tggaactgct cttgtcatat aaggccgtta
     1741 acacacactg tggatctaaa ccaccattct cattttctga ccaggccact actttgtaaa
     1801 cgtgagcacc ggactcaaaa gatgcgaaaa aatataatcc cggttcagct ggtaggatct
     1861 tcattagcta gcttcctttt gtttattctg tctgagaggc aataactacc taattccccg
     1921 tggtgggtat aaaaaatcac ggggaggtta ataacctaaa cgttttagtt ctgctgctaa
     1981 acgcttaaga ctctcatcta aatactcgtg attctctttt agctcgtttt tcattaaatc
     2041 aatgtgacga gataactcag gattgcctcc tgaacgactg accggaaagt cgtcgctgga
     2101 agataagcgg ccatgtttac tgtaatccat tgctatgcag ataagcgttg ccaagtcgat
     2161 aacacgatga agaggtaatt cttctgattg ccgtgaccac ttttctccgg tgttgcgcca
     2221 cacttttgct gatagctcgg tatacccggg ggtgttccat tgagcaattc caacggaaag
     2281 ccccatagca tcagtgtcat cagcataagg tccatcaatt cgatcatagt tttctacagc
     2341 gataatcggt ttgtgtttca gatgagtagg gatgttcata tcattctctt ttatgtgaat
     2401 ttaggttgtt aattaataaa ataacaaaac aacttatttt actaattagt aagtgtaaca
     2461 ttttgtgaat tgatggattt gaagttgttg gcgtggatct tcttgagatc atttatggct
     2521 gagcgtaatc tcttgctctg aaaacgaaaa aaccacctgg ggaggtggtt tgatcgaagg
     2581 ttaggtcagt tggggaactg cttaaccagg taactgtctt gtgcgagacg agtcaccaaa
     2641 actgtccttc cagtgtagcc gttctcaggc tcccacttca agaactccag ataaatctct
     2701 cgcacatctc agttaccagt ggctgctgcc agtggcgctt ttgcatgtct ttccgggttg
     2761 gactcaagac gatagttacc ggacaaggcg cagcagtcgg actgaacggg gggttcgtgc
     2821 atacagtcca tcctggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
     2881 caaacgcggc cataacagcg gaatgacacc ggtaaaccga agggcaggaa tgcgggggag
     2941 cacgagggag ccatcagggg gaaacgcctg gtatctctaa gccgcatcgg gtttcgccac
     3001 cactgatttg agcgtctgat tttgtgatgc tcggcagggg ggcggagcct atggaaaaac
     3061 ggcaacgccg catccttttt cagcccttcc tgatttccgc ttagtctctt tatccagcca
     3121 ttcgtgagct ggcaacgccc gccgcagtct cacggcagga gcgcagcgac cgagtgagcg
     3181 aggaggcgtc atttcaccgg gatctgcatt tcgcacttac acaccggtgc tgcatttccc
     3241 ggtaccacat gaagcacttt cgtaaaccat tactgcgttg taacatttta agccagtata
     3301 cactccgcta gcgctacgtg actggttcag ggctgcgccc cgaaacctgc taaaaccact
     3361 gacgcgcctg cggcttgtcc aacaccgcgc cgaccgggaa aagatttccc gcccgtcccg
     3421 gcgttatcag gaggccggat tggcagacaa acgcagcaaa atgctcacga tgtgggtgac
     3481 cgaagatgag caccgccgtc tgctggagcg ctgcgacggt aaacagcttg cagcctggat
     3541 gcgccagacg tgcctggacg agaaacccgc ccgcgccggc aagcttccct cgctctcgcc
     3601 ggcgctgctt cgtcagcttg ccggcatggg gaacaacctc aaccagattg cccggcaggt
     3661 taacgccggt ggtggcagcg ggcacgaccg cgtgcaggtc gtcgccgcgc tgatggccat
     3721 agatgccgga ctcgagcggc tgcggcatgc cgtgctggaa aagggtgcag acgatgatcg
     3781 ttaagtttca cccgcgaggg cgcggcggtg gcgccggtcc ggtggattat ctgctgggga
     3841 aagaccgaca gcgcgacggc gccaccgttc tgcaggggaa gccggaagaa gtcagggagc
     3901 ttatcgacgc ctcgccctac gcaaaaaagt acacatccgg cgtcctgtcc tttgccgaaa
     3961 aggatttacc gcccgggcag cgcgaaaagc tgatggcgag ctttgaacgt gttctgatgc
     4021 ccggactcga taaagaccag tacagcgtgc tgtgggttga gcaccaggat aaggggcggc
     4081 tggagctgaa cttcctgatc ccgaacacgg agctgctgac cggcaagcgt ctccagccgt
     4141 attacgaccg cgccgaccgt ccgcgcattg atgcctggca gaccatcgtg aacggcaggc
     4201 tggggctgca tgacccgaac gcgcctgaaa accggcgggc gctggtcacg ccgtccgcgc
     4261 tgccggaaac gaagcaggaa gccgctcagg caattacacg gggtttactg gccttagcct
     4321 cgtccgggga gctgaaaacc cgccaggacg tcactgaggc gctggaaagc gcaggttttg
     4381 aggtggtacg caccacaaaa agcagcatca gcattgccga cccggacggg gggcgaaaca
     4441 tccgactgaa gggagccatc t
//
