LOCUS       NZ_CP083712             4938 bp    DNA     circular CON 09-AUG-2023
DEFINITION  Enterobacter cloacae complex sp. ECL352 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP083712
VERSION     NZ_CP083712.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21399027
            Assembly: GCF_020149545.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae complex sp. ECL352
  ORGANISM  Enterobacter cloacae complex sp. ECL352
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Zhang,X.
  TITLE     Molecular characterisation of NDM-1-Producing Carbapenem-Resistant
            E. cloacae complex From a Three Tertiary Hospitals in
            Chongqing,China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2021) Department of Microbiology, Yongchuan
            Hospital of Chongqing Medical University, Xuanhua Road, Yongchuan
            District, Chongqing 402106, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 550.0x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/07/2023 02:05:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,906
            CDSs (total)                      :: 4,788
            Genes (coding)                    :: 4,679
            CDSs (with protein)               :: 4,679
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 28 of 109
            Pseudo Genes (incomplete)         :: 80 of 109
            Pseudo Genes (internal stop)      :: 21 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            Pseudo Genes (short protein)      :: 1 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083712.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Enterobacter cloacae complex sp. ECL352"
                     /mol_type="genomic DNA"
                     /strain="ECL352"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:2876028"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:Yongchuan District, Chongqing"
                     /collection_date="2020-09-24"
     gene            complement(798..2102)
                     /locus_tag="LBF07_RS24530"
                     /old_locus_tag="LBF07_24530"
     CDS             complement(798..2102)
                     /locus_tag="LBF07_RS24530"
                     /old_locus_tag="LBF07_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_007899670.1"
                     /translation="MFSLKNMFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFL
                     STMGYRADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLE
                     ASYIPVSDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEAN
                     ENFVNATGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDP
                     LWFRATYNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGA
                     NVIENSILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEA
                     ARAGASGRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREG
                     VTLMREAGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            complement(3013..3228)
                     /locus_tag="LBF07_RS24535"
                     /old_locus_tag="LBF07_24535"
     CDS             complement(3013..3228)
                     /locus_tag="LBF07_RS24535"
                     /old_locus_tag="LBF07_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3235..3726)
                     /locus_tag="LBF07_RS24540"
                     /old_locus_tag="LBF07_24540"
     CDS             complement(3235..3726)
                     /locus_tag="LBF07_RS24540"
                     /old_locus_tag="LBF07_24540"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_016355729.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESNDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<3761..4195)
                     /locus_tag="LBF07_RS24650"
                     /pseudo=""
     CDS             complement(<3761..4195)
                     /locus_tag="LBF07_RS24650"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4404..4727)
                     /locus_tag="LBF07_RS24550"
                     /old_locus_tag="LBF07_24550"
     CDS             complement(4404..4727)
                     /locus_tag="LBF07_RS24550"
                     /old_locus_tag="LBF07_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007899679.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTAKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGVGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4843..4938)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.820000000000001e-43"
     Typing          2..548
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.545"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(2913..4414)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.692"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4411..4727)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="3.03e-59"
ORIGIN
        1 atgtggcagg agaaaaatgc agcagcggag cgtcagcaga atatgcgata caggatatat
       61 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtaccg
      121 gcttacaggc ggggcggaaa tttcctggga gatgccagaa agaagcttaa cagggaagcg
      181 ataaggccgc ggcgaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
      241 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      301 cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      361 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg gaaggcagtt
      421 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc
      481 ggtaactatc aacttgagtc caatccggaa aaacacgaca aagcgccact ggcagcagcc
      541 actggtaaca ggtttagagg aggagagtct tgaagtttcg ggtgcgataa ggctacactg
      601 aaaggacagt tttggtgact gtgctcctcc aagccagtta ccgcggttca aaagagcgtc
      661 aactcttgcg aaccttcgaa aaagccacct tgccgggtgg ttttttcgtt ttcagagcaa
      721 gagattacga cgcaacggaa aggatctcaa gaagatcatc tgtgatgttc gacagatatg
      781 gggatatgca ccatggccta tccggcttta actgaaatgg cgacatcctt aaccgcagtc
      841 tcaacccctg cagcattcct ctgaatgctg gtcataactt cacccgcttc gcgcatgagt
      901 gtaacccctt cccgagtgtt catcaggctg ttttcaatgc cacagagaac atccttggcc
      961 agttgactat tgctggccac gacttgctca atttcttcag tcgcgctact gacgctcgcc
     1021 gcaagattac gcacttctgc ggcaacaacc gcaaaacttc ttccggatgc gccagccctt
     1081 gctgcttcaa tcgcggcatt cagtgcaata agtcttgtct gcatggcaaa cttccggatg
     1141 gtttccacca tatcgtcaat actttcagac tgattgttga gccgtgagat atcggtggag
     1201 acagccccca tcccctgcgc aattctgtca atcataagga tgctattttc aataacattg
     1261 gcaccgttct gcgcactttc tcgggtctgc acggccatat cccatgcatg cacagcagcc
     1321 tcttgctctc tctggtttcg caggacatca gccgtaacat ctgtagcgaa tttcacaatt
     1381 ttataaagct gcccatcact gttaaagaca gggttataag ttgcacggaa ccacagaggg
     1441 tctccctgac ggttaaggcg tggaaacaga ccagaaaaaa actcaccttg attcagactc
     1501 tcccagaaat gtcggtattc atcacttttg taaagcgtct cggcacagaa cagacgatga
     1561 tgcttaccta tgatttcatc ccgcttatag ccagtggcat tgacgaaatt ttcatttgct
     1621 tcaagcacga tcccttcagg attgaaggtg atgatggcca tagaacggtt tatggcattt
     1681 acgactgctt cctgctcaag agcagaatgc acgcgctcag aaatatcggc agcaatttta
     1741 atgactctga taacacggcc gcgcctgtca gaaacgggga tataactggc ttcaagccat
     1801 acggaacgat ttccctttgc cagccgcagg tacttgccac taaagctttc tcccctggca
     1861 aggcgctgcc agaactggct gtattccggt gaacttacca gcgagggcgg gcagaatata
     1921 cgatggtggt gaccgattat ttcatcagcc ctgtacccca tagtggagag aaaaagcggg
     1981 ctggctttat ttatcacccc ctccggactg aactcaatga caggcttatt ctgacaaatt
     2041 gcgtcatacg aaatggctga tcgctggagg ttcagggaga acatattttt aagtgaaaac
     2101 atatgaatca gattgcccgg ataatgtaag tactgccatg gttatcggct actccttatg
     2161 tagcgttata tattactgcg acagggcggg aaaaagcccg ctcttcagcc atgccggcat
     2221 gatgtccggc tccggatttc acgcgtaaaa aacccgccat ctttagcggg ctgaacaata
     2281 agaaaatgtt catatttacg gcaaatccgc cggctccagc agagaagctg gcgtctatca
     2341 atcttggaag aaccagcgca gctccgcatg cattaacagg catcagaagg caaaaaaatg
     2401 gctcacatcc gggagccgga aaacactcaa ctgcctggat cgcagcacat cctttgtgaa
     2461 ggataattta agtatcggcc ggattgccgc gggcataagc ccggccggca taattttttc
     2521 cgtatccgcc agacctcggc ttttaacata acaccccttg cccgcaccgc agaaacgggg
     2581 ttggctgcgt ttccggtgcc agacgcgaga aagtggcgac agacatgacg aaaaaacacc
     2641 aaaaacgcag ccgctggata ttacactgat gcatagtcat gcagccagat gcatgcagcg
     2701 taaagtcaca ctaaaatcag cgcgaacgcc ttaaaaaccg gcaaatgcct ctccggcaac
     2761 tcacacagct ccatcaccag gtaaaaacag ttcagcaggg cttcaataac gttgagaatt
     2821 ttcatcactt cctccgggat aagtcactac cattccggta gtcagcctat ttagcaggaa
     2881 cgcgatgagc ccttcttcgt gcgggctgtc acctcatctt cggaccatcg aaactcttct
     2941 gcttctgtcg gagcgtcagc tccagctcgt tctgacgctg atactccagt gcagccttct
     3001 cctcgatggc attcagttcc cggacaagcg gcgcagctgc tcgcttaaac tcagcacctg
     3061 cgtgctcaag tccgacacgc gctcgttcag caccgcgttc tcccgttgca taagtccaca
     3121 catgctccgc cattccgcga aggcgctctc ccattcgtcc agcctttgcg agtagtcctg
     3181 ctgtagctgc tctaatgcgc tcagcaactg cttttccagc tctgtcattt tccgctactc
     3241 ctgtttcagg cggatccagg tcgtcccgtc gtaggggatg gcttcggctt gcatccccgg
     3301 cggcaggata aggaacctcc ggccgtcgtt gctctcctga tagcgtactc cccacgtccg
     3361 ggcggtcatt ttcgccaggg tatcttcctg ctgacgaaga tgtgtgtaat tgtcggttat
     3421 ctgccgtccc tgccaccaca gaatgctccc gctcgtggcg atcagcagcg ctgacaccat
     3481 caggatggtg agccatgtcc tcccggccgt cagcaacacg ctccgccggt ttttctccat
     3541 cgcctcgctc atactctgct cgtgggcgct gatggcgtcg ctgattctcc tcgcgcttgc
     3601 gttcagttcc gctctgacag actgctcgtg ctcgctgaat gcggctttca gcatctcgcc
     3661 ggtactcttc tgctgcgctt tcgattgctg ctctaagtcc ttcgccagcg ttaaaagact
     3721 gctcatagat ggctcccttc agtcggatgt ttcgcccccc gtcagggtcg gcaatgctga
     3781 tactgctttt cgttgtacgc accacctcaa aacctgcacc ttcaagagcc tcagtgacgt
     3841 cctgacggct tttcagctcc ccggacgagg ctaagctcag cagtccacgg gtgatggctt
     3901 ctgcggcttc ctgcttcgtt ttcggcagtc cggccggcgt gaccagcgcc cggcggtttt
     3961 ccggcgcgtt cggatcgtgc agccccagtc tgccgttcac gatggtctgc caggcatcga
     4021 tacgcggccg gtcggcgcgg tcgtagtacg gctggagccg cctgccggtc agcagctcgg
     4081 tattggggat cagaaagttc agctccagac gccccttgtc ctcgtgctcc acccacagaa
     4141 tgctgtactg atctttatca agtccgggca tcagaacccg ctcgaagctg gccatcagtt
     4201 tttcccgctg gccgggaggc agatcggcct ccgcaaacga caggacgccg gaggtgtatt
     4261 tcttcacgta aggcgacgcg tcgatcagct cccggacttc ttccggcttc ccctgcagga
     4321 cgctggcgcc ttcacgctgc cggtctttac ccagcagata gtccaccggc ccggcgccac
     4381 cgccgcgacc gcgtggatga aatttaacga tcatcgtccg ttcctttctc aagaacggcg
     4441 tgccgcagcc gctcaagccc ggcatcgatg gccatcagcg ccgccacaat ctgcacccgg
     4501 tcatgaccgg taccgacgcc ggcgttaaca cggcgggcta tctggttcag gttgttgccc
     4561 ataccggcaa gctggcgcag cagcgccggc gagatcgaag gaagttttgc ggtacgagcc
     4621 ggcttttcgt ccaggcaggt ctgccgcatc cacgccgcca gctgcctgcc gtcgcagcgc
     4681 tcaagtagcc gcctgtgctc atcctccgtc acccacatgg tgagcatctt gttccgtttc
     4741 tctgacaaaa cagtctcctg ataaccccgg gacgggcggg aaaacctttc ccggtcggcg
     4801 cggggttgga caagccgcag gcgcgtcagt ggctgttagc gggtttcggg gcgcagccct
     4861 gaaccagtca cgtagcgcta gcggagtgta tactggctta ctatgttggc acggatgcga
     4921 atatcagtga agtgcttc
//
