LOCUS       NZ_CP083708             2634 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Escherichia coli strain SUISSEKPC3NDM5 plasmid p1606g, complete
            sequence.
ACCESSION   NZ_CP083708
VERSION     NZ_CP083708.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21369264
            Assembly: GCF_020149465.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Sadek,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2021) Microbiology, University of Fribourg,
            Faculty of Scienec and Medicine, Boulevard de Perolles 64, Fribourg,
            ? 1700, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-MAR-2021
            Assembly Method :: CLC Genomic Workbench v. version 20.0.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiniSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 02:32:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,532
            Genes (coding)                    :: 5,075
            CDSs (with protein)               :: 5,075
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 457
            CDSs (without protein)            :: 457
            Pseudo Genes (ambiguous residues) :: 0 of 457
            Pseudo Genes (frameshifted)       :: 228 of 457
            Pseudo Genes (incomplete)         :: 222 of 457
            Pseudo Genes (internal stop)      :: 74 of 457
            Pseudo Genes (multiple problems)  :: 57 of 457
            Pseudo Genes (short protein)      :: 4 of 457
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083708.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SUISSEKPC3NDM5"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p1606g"
                     /geo_loc_name="Switzerland: Basel"
                     /lat_lon="47.55839 N 7.57327 E"
                     /collection_date="2020-11-06"
     gene            <110..343
                     /locus_tag="K9O95_RS28060"
                     /old_locus_tag="K9O95_28080"
                     /pseudo=""
     CDS             <110..343
                     /locus_tag="K9O95_RS28060"
                     /old_locus_tag="K9O95_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            506..658
                     /locus_tag="K9O95_RS28065"
                     /old_locus_tag="K9O95_28085"
     CDS             506..658
                     /locus_tag="K9O95_RS28065"
                     /old_locus_tag="K9O95_28085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224451535.1"
                     /translation="MLTLCEWYIFPDYRPEPAAGSLSTASLCSLLNASQTRIKIRIRSP
                     FFKVR"
                     /name="hypothetical protein"
     gene            1212..2178
                     /locus_tag="K9O95_RS28070"
                     /old_locus_tag="K9O95_28090"
                     /pseudo=""
     CDS             1212..2178
                     /locus_tag="K9O95_RS28070"
                     /old_locus_tag="K9O95_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     Typing          1607..1838
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gaatatcgat attcgggcag taaagcgaac aggtatgagg ttgtgactta ttgttgctga
       61 cgcgcaacag ttgctgtaaa gctttaatac ttcgttaaac cggtatttat tggcaaacga
      121 tcctgtgtgg tttcttgagc tgacgcggca gcttcacaag cgccgtttta tctcgaccgg
      181 tggggcgcta aaaaacgtcc tccagttgga tcgagaaacc aatgagatct tgtcattgcc
      241 gacgatgtag gggatggcac tgatgacggg aagcggacgg cgtttgtctg ggattcaggt
      301 aaacggcgtt acaaacgcgc ccctgaagga taaatcggat taacgtatga atattaatat
      361 tgaatacctg aatggaaata agactattgg tttatttttt ttaagaagtg aagcggtgat
      421 tctgacaggt ttaaaaacct tattttgctt attgatggat taagttttgg cacatttggt
      481 ttcatccgca cgaaggtttt gaggaatgtt aacactctgc gagtggtaca ttttcccgga
      541 ttatcgtcct gagcctgccg ctggctctct ttctaccgcc tcgctttgct cgttgctcaa
      601 cgcctcacag acacggatta aaatccgcat ccgttcaccg ttttttaaag tccgttaaaa
      661 gcatgatgcc atctccgaga gttaatctcg tcaaatgcta aatcgtgggg gtcccctttg
      721 gggttccgat ttagtgattg acgacaccac cgattaaaaa acttatgcgg ggtggatggt
      781 ttcacgaagt gaggccatcc acctgtaaga cagggttttg tttttattcc ctgttttggt
      841 gatcgggtgt gtggaaaagg ttggggtaag ccgttcgggg gtgcttgttt tgggggttaa
      901 aattgtggtt attttttgcg caattctcgc gcgtgatcct tgtatttata cttaagggat
      961 aaatggcgga tatgaaatag tggtttagcc cagtaatgac gaggcctttg agtgggtttt
     1021 gacaggtcaa agaaaatgga gcagaattga ggcgttttta atcggcgttg gggagtgcgt
     1081 caacactccc caacatttcg aatgtgtcac ctcagcggca aactctggtg acatgtactg
     1141 gctcgcaatg cacaggtacg tgatgaatat accacatcaa atcacagcct gcccagatcg
     1201 gagcaggctt aatgtcagaa gataaattcc tttcggacta cagcccccgt gatgcagttt
     1261 gggataccca gcgcacgctt accgattctg tcgggggtat ctaccagact gctgctgaat
     1321 tcgagcgcta tgcactccgt atggcctcct gtagcggttt gttacgtttt ggttggtcta
     1381 ccatcatgga aaccggagaa acgcgcctac ggcttcgtag tgcgcaattt tgccgtgtcc
     1441 gtcattgccc tgtctgccag tggagaagaa ccctcatgtg gcaagcccgt ttttatcagg
     1501 ctctaaccga aaatcgttgt ggattacccg tcttcccgat ggttgtttct gacgttaact
     1561 gtcaggaact gcgagatagg tgaacttgga acagtcctta cagcaatgaa tgcggcgttt
     1621 aagcgaatgg aaaagcgaaa ggagctatca cctgttcagg ggtggatcag ggctacggag
     1681 gtgacgcgag gtaaggatgg cagcgcacat ccgcattttc actgtctgct gatggtgcaa
     1741 ccttcttggt ttaaagggaa gaactacgtt aagcacgaac gttgggtaga actctggcgc
     1801 gattgcttgc gggtgaacta tgagccgaat atcgatattc gggcagtaaa aactaagaca
     1861 ggtgaggttg tggccaacgt tgccgagcaa ctgcaaagcg cggttgctga aacgctgaaa
     1921 tactccgtta aaccggaaga tatggcaaac gatcctgagt ggtttcttga gctgacgcgg
     1981 cagcttcaca agcgccgttt tatctcgacc ggtggggcgc taaaaaacgt cctccagttg
     2041 gatcgagaaa ccaatgagga tcttgtcatt gccgacgatg taggggatgg cactgatgac
     2101 gggaagcgga cggcgtttgt ctgggattca ggtaaacggc gttacaaacg cgcccctgag
     2161 aaggataaat cggattaacg tatgaatatt aatattgaat acctgaatgg aaataagact
     2221 attggtttat ttttttaaga agtgaagaat ctctagtgct ctgtctatcc tgcattatcc
     2281 tcagcattat cctcagcctt gccaactcga caccaatgca ggatagacaa tccgatgtca
     2341 aatgttaaca ctctgcgagt ggtacatttt cccggattat cgtcctgagc ctgccgctgg
     2401 ctctctttct accgcctcgc tttgctcgtt gctcaacgcc tcacagacac ggattaaaat
     2461 ccgcatccgt tcaccgtttt ttaaagtccg ttaaaagcat gatgccatct ccgagagtta
     2521 atctcgtcaa atgctaaatc gtgggggtcc cctttggggt tccgatttag tgattgacga
     2581 caccaccgat taaaaaactt atgcggggtg gatggtttca cgaagtgagg ccat
//
