LOCUS       NZ_CP083615             4783 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533 plasmid
            unnamed2, complete sequence.
ACCESSION   NZ_CP083615
VERSION     NZ_CP083615.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21402700
            Assembly: GCF_020097195.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. oharae
  ORGANISM  Enterobacter hormaechei subsp. oharae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Hazen,T., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Hazen,T., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.8, SPAdes v. 3.14.1
            Genome Coverage                   :: 1403.75x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020097195.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 04:32:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,452
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 23 of 62
            Pseudo Genes (incomplete)         :: 36 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            Pseudo Genes (short protein)      :: 1 of 62
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083615.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterobacter hormaechei subsp. oharae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1533"
                     /sub_species="oharae"
                     /culture_collection="DSM:16687"
                     /type_material="type strain of Enterobacter hormaechei
                     subsp. oharae"
                     /db_xref="taxon:301102"
                     /plasmid="unnamed2"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            complement(join(4534..4783,1..107))
                     /locus_tag="LA353_RS22855"
                     /old_locus_tag="LA353_22850"
     CDS             complement(join(4534..4783,1..107))
                     /locus_tag="LA353_RS22855"
                     /old_locus_tag="LA353_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(310..627)
                     /locus_tag="LA353_RS22860"
                     /old_locus_tag="LA353_22855"
     CDS             complement(310..627)
                     /locus_tag="LA353_RS22860"
                     /old_locus_tag="LA353_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            814..1056
                     /locus_tag="LA353_RS22865"
                     /old_locus_tag="LA353_22860"
     CDS             814..1056
                     /locus_tag="LA353_RS22865"
                     /old_locus_tag="LA353_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(1727..1942)
                     /locus_tag="LA353_RS22870"
                     /old_locus_tag="LA353_22865"
     CDS             complement(1727..1942)
                     /locus_tag="LA353_RS22870"
                     /old_locus_tag="LA353_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1949..2440)
                     /locus_tag="LA353_RS22875"
                     /old_locus_tag="LA353_22870"
     CDS             complement(1949..2440)
                     /locus_tag="LA353_RS22875"
                     /old_locus_tag="LA353_22870"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2475..3179)
                     /locus_tag="LA353_RS22915"
                     /old_locus_tag="LA353_22875"
                     /pseudo=""
     CDS             complement(<2475..3179)
                     /locus_tag="LA353_RS22915"
                     /old_locus_tag="LA353_22875"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3118..3441)
                     /locus_tag="LA353_RS22885"
                     /old_locus_tag="LA353_22880"
     CDS             complement(3118..3441)
                     /locus_tag="LA353_RS22885"
                     /old_locus_tag="LA353_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4092..>4214
                     /locus_tag="LA353_RS22920"
                     /pseudo=""
     CDS             4092..>4214
                     /locus_tag="LA353_RS22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(3557..3730)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          3654..4201
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.559"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(1636..3128)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3125..3441)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.45e-59"
ORIGIN
        1 acattcaaca tttcagccaa gtcattgatt tttaaaccac ttggttcgag atattcataa
       61 agaataatat cccctacagt tgatggtttt ctagttgctt gggacatgct ttactcctaa
      121 attttttact tctctctgtt agatgaggaa gataagtatt tcgtctatta ccagctgtag
      181 cgatgagtaa ttatcttaaa tatagtcacg tcattttttt tattaatcat tatttgtaat
      241 gacattttaa tgtaggtgag ggaacgcctc acctacttgt tttataagat aacagccacg
      301 acctgttatc tatcggtaat catgtggatc gagatacaaa tcctcgactc cgttttcacc
      361 ccacttaaat atcaagcggt acttctcatt cacccgtatg gatgaatacc ctgtcaatgg
      421 agggttgaga ttctcaaatc ggtttcccgg gggagaccgc aaatctccaa ctttatcagc
      481 agcattcagc atatccagct tgcgacctaa tgaagcacta atcgcagttg gaatctttcg
      541 attggcttta ccatatttgt aaaactggta tagccaatcg tctcgaaact gaatgcggca
      601 atcatcatgt tcatgatctt gctgcatttt tcttttacct caatttttgc cgcaacttgg
      661 caattttttc gatcaccttc ttacctccga tatagtacag caaacatatc aaatgcacaa
      721 cagcacagtg cacttgtgca atcaatggct atgctgtaga atgcactcat gcacagtgcg
      781 ggtgtgcaaa ataaatgata atttggaggt tgcatggctc ttgttcgctc aggaaacaaa
      841 agcaagattt ttgaagcaga tacgaagttt aactcggttt ttgacactga atataagccg
      901 ggagaaacat gtgaatatga agggatttat aaatgtaccg gttgcggtaa agaagtaact
      961 atcgcaggcg ggaaaacaat cccaccacaa aaccaccacc aacatacccc cgcgcaaggg
     1021 aacattcgtt ggcaattgtt tgtttatgct cagtgaggta tagaaaatgg cagatcgtcg
     1081 accactcaac ccacacgttg tcgttcctaa accaggcggc ggaactcaac ctcgtgaccg
     1141 taacaatgac ggaagggtca gaggaaaacg ctcagatgca ggaaagccac gaaaataaca
     1201 gcaagccgcc agcctggcgg cttttttata tctgcagctg atactgattt accataacat
     1261 ccgttgggcg caccgctgaa acggggttgg ctgcgtttcc ggtgccagac gcgagaaagt
     1321 ggcgacagac ataacgaaaa aacaccagaa acgcagccgc tgaatattac actggtgcat
     1381 agtcatgcag ccagatgcat ggatgttaaa gtcacactaa aatccgcgcc aatgccttaa
     1441 aaaccgacaa atgcttctcc agccagtccc acagctccgc cgccagatac aggcagttaa
     1501 gcaatgattc aatgagattt aacagcttca tttcccgctc ctgaataagt cgtgaccttt
     1561 ttgatcacga acctatttag cgggagaaat aagagcccct cttcgtgcgg gttgtcagct
     1621 gcctggatgg tttaaagtgt cggcccgcgg taggttttct gcggctggcg ctccagctcc
     1681 agtgttttct gatactcatt ctccctctgc cggagctcga tggcattcag ttcccggaca
     1741 agcggcgcag ctgctcgctt aaactcagca cctgcgtgct caagtccgac acgcgctcgt
     1801 tcagcaccgc gttctcccgt tgcataagcc cacacatgct ccgccattcc gcgaaggcgc
     1861 tctcccattc gtccagcctt tgcgagtagt cctgctgtag ctgctctaat gcgctcagca
     1921 actgcttttc cagctctgtc attttccgct actcctgttt caggcggatc catgtcgtcc
     1981 cgtcgtaggg gatggcttcg gtttgcatcc cgggcggcag gataagaaac ctccggccgt
     2041 cgctgctctc ctgatagcgc actccccacg tccgggcagt cattttcgcc agggtatctt
     2101 cctgctgacg aagatgcgtg taattgtcgg ttatctgctg cccctgccac cacagtatgc
     2161 tcccgctcgt gccgatcagc agcgctgaca ccatcaggat ggtgaaccat gtccgcccgg
     2221 cagtccgcag tacgctccgc cggtttttct ccatcgcctc gctcatactc tgctcgtgtg
     2281 cgaggatggc gtcgctgatt ctcctcgcgc ttgcgctcag ttccgctctg acagactgct
     2341 cgtgctcgct gaatgcggct ttcagcatct cgccggtact ctgctgctgc gctttcgatt
     2401 gctgctctaa gtccttcgcc agcgttaaaa gactgctcat agatggctcc cttcagtcgg
     2461 atgtttcgcc ccccgtccgg gtcggcaatg ctgatactgc ttttcgtggt gcgcaccacc
     2521 tcaaaacctg accgttccag cgcctcagtg acgtcctgac ggtttttcag ctccccggac
     2581 gaagcaaggg tcagcagtcc gcgcgtgatg gcttcagtgg cctcctgctt cgttttcggc
     2641 agtccggacg gcgtgaccag cgcccggcgg ttttccggcg cgttcgggtc atgcagcccc
     2701 agtctgccgt tcacgatggt ctgccaggca tcgatgcgcg gccggtcggc gcggtcgtag
     2761 tacggctgga gccgcctgcc ggtcagcagt tcggtattgg ggatcagaaa gttcagctcc
     2821 agacgcccct tgtccgcgtg ctccacccac agaatgctgt actgatcttt atcgagtccg
     2881 ggcatcagaa cccgctcgaa gctggccatc agtttttccc gctggccggg aggcagatcg
     2941 gcctccgcaa acgacagtac gccggaggtg tatttcttca cgtaaggcga cgcgtcaatc
     3001 agctcccgga cttcttccgg cttcccctgc aggacgctgg cgccttcacg ctgccggtct
     3061 ttacccagca gatagtccac cggcccggcg ccaccgccgc gaccgcgtgg atgaaattta
     3121 acgatcatcg tccgttcctt tctcaagaac ggcgtgccgc agccgctcaa gcccggcatc
     3181 gatggccatc agcgccgcca caatctgtac cctgtcatgg ccggtgccgc cgccggcgtt
     3241 aacacggcgg gctatctggt tcaggttatt gcccataccg gcaagctggc gcagcagcgc
     3301 cggcgagagt gaggggagct ttccggtgcg cgcaggcttt tcgtccaggc aggtctgccg
     3361 catccacgcc gccagcggcc tgccgtcgca gcgctcaagc agccgccggt gctcgccctc
     3421 cgtcacccac atggtgagca tcttgttccg tttctctgac aaaccggcct cctgataacg
     3481 ccgggacggg cgggaaagcc tttcccggtc ggcgcggggt tggacaagcc gcaggcgcgt
     3541 cagtggcttt tggcgggttt cggggcgcag ccctgaacca gtcacgtagc gctagcggag
     3601 tgtatactgg cttaatatgt tggcacggat acggaaatca gtgaagtgct tcatgtggca
     3661 ggagaaaaaa ggcggcaacg gcgcgtcagc agaatatgcg atacaggata tattccgctt
     3721 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggtg cgggcttacg
     3781 aacggggcgg aaatttcctg gaagatgcca ggaagaaact taacagggaa gcgataaggc
     3841 cacggcagag ccggttttcc ataggctccg cccccctgac gagcatcaca gaatctgacg
     3901 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctga
     3961 tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     4021 atggccgcgt ttgtctcatt ccacgcctga cactcggttc cgggtaggca gttcgctcca
     4081 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctgt tccggtaact
     4141 atcgacttga gtccaacccg gaaagacacg acaaaacgcc actggcagca gccactggta
     4201 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4261 actacggcta cactggaagg acagtatttg gtatctgcgc tctgctgaag ccagttacca
     4321 ggttaagcag ttccccaact gacttaacct tcgaaaaacc gcctccccag gcggtttttt
     4381 cgtttacaga gcaagagatt acgcgtacag agcacggatc tcaagaagat cgtaagaaga
     4441 aagttaacat gagcgttttt atccctcaaa aagaaggctt ctagttatag aagccttctt
     4501 tttgagggat aacttatatt tttttaaaaa aaatcatgca atagcatgtt tcttctcaga
     4561 actatcaaca atgtgtttcc tttctatgat ttcatgagcg ggaatcactc tgtctatttc
     4621 ttcttgcaat cgagggctac tagtaacctc ccataaatca acagactgct gtatatttag
     4681 ccagaacttt gcagtggtat cgaaggcttt agccaaccta aaagccatat cggtagacag
     4741 ctttctatta ttgttaacta aagcactggc agagtttcta tgc
//
