LOCUS       NZ_CP083614             2495 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533 plasmid
            unnamed1, complete sequence.
ACCESSION   NZ_CP083614
VERSION     NZ_CP083614.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21402700
            Assembly: GCF_020097195.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. oharae
  ORGANISM  Enterobacter hormaechei subsp. oharae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Hazen,T., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Hazen,T., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.8, SPAdes v. 3.14.1
            Genome Coverage                   :: 1403.75x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020097195.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 04:32:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,452
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 23 of 62
            Pseudo Genes (incomplete)         :: 36 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            Pseudo Genes (short protein)      :: 1 of 62
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083614.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei subsp. oharae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1533"
                     /sub_species="oharae"
                     /culture_collection="DSM:16687"
                     /type_material="type strain of Enterobacter hormaechei
                     subsp. oharae"
                     /db_xref="taxon:301102"
                     /plasmid="unnamed1"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            279..464
                     /locus_tag="LA353_RS22840"
                     /old_locus_tag="LA353_22835"
     CDS             279..464
                     /locus_tag="LA353_RS22840"
                     /old_locus_tag="LA353_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(755..973)
                     /locus_tag="LA353_RS22845"
                     /old_locus_tag="LA353_22840"
     CDS             complement(755..973)
                     /locus_tag="LA353_RS22845"
                     /old_locus_tag="LA353_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            2181..2408
                     /locus_tag="LA353_RS22850"
                     /old_locus_tag="LA353_22845"
     CDS             2181..2408
                     /locus_tag="LA353_RS22850"
                     /old_locus_tag="LA353_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          complement(512..599)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          1238..1766
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 ctaaaagctt tcagggttgt ctaaaaagca caagacataa gaggaagtgc ggtattttat
       61 aatcaagccc ccaagaattt accataacat ccgttgcccg caccgcctga gacgcgttca
      121 gcgcattcct gacgaaacca tgacaaaaac cacaacaaac caccccggaa cccgtcagaa
      181 acgcgcctgt taaattttaa cggcatgcat gactatgcac cagaatgacg ccatgctctt
      241 ttcacgcaaa aatcatcacc agacggggaa aatcaccagt gaccagacag gaatccgccg
      301 ccctcaatat ggccaaattt atccgcgcac agacacttct cctccttgag cggctcgagc
      361 agatggatct ggatgaggct gccggctgct gtgagcacct gcacgatcag gccgaagcgc
      421 tttacgccat gctgaacgca cagataggcg aggaaaatgc gtgaaaatcg gtgaacgggt
      481 gcgcaattca gtgcgcggcc gtgaggcgat ggcggggtgt cggggcgcag ccctgaccag
      541 ggtatttgtg atgccggcgc gtgcgcggta ttacaaatgc acatcctgtc ccggaacgga
      601 caccgggaaa cagcaaaaaa aaccgggcgg cacgcccgga actcaatcaa gttagattag
      661 attactctca ctcgtccata acagcatcat ggaacgacga ccaccgtccg tgacggccgc
      721 ctcgtttaag tatggacaga aatacagaaa atgctcagga cgaaatgtaa tgaatgcgaa
      781 cggattcaag aaattcgagc atgacagtcc ttacggccgg ttcggtttca gacaaaatct
      841 gccggtatgc atccagcatc atggctccgg catcccctcc ggcacgccgt agccagaccg
      901 aaacaacgga cacaagcagg tgtcgctcat catcactaag agtcatcagg gctccggaag
      961 aaaaaccaaa catggcttac ctactgttaa cggcaacgca gaagaaaggt ttacagggta
     1021 gcgggcaggg atgaaaaaaa aaaaattgca gtcaggccgg aacggctgct gaaccggtac
     1081 aggcgggttc cgggcgcagc gccggaccag tcacggagca ctagcggagt gtatactggc
     1141 ttaagatgta gcatggcagt aacacatagc attaatgcca cataagacag gcgcagaaag
     1201 tgaaagaaaa cgcgtcagga gaaaatgtga tacagaatat attccgcttc ctcgctcact
     1261 gactcgctcc gctcggtcgt tcggctgcgg cgagcgggac gggcttacgg atggatcaga
     1321 aatttcctgg gggatgccag gaagatactt aacgaagaaa gcataaggcc gcgccaaagc
     1381 cgtttttcca taggctccgc ccccctgaca agcatcacag aatctgacgc tcaaatcagt
     1441 ggtggcgaaa cccgatgcgg cttaaagata ccaggcgttt ccccctggtg gctccctcgt
     1501 gctcccccgc attcctgcct ttcggtttac cggtgtcact ccgctgttat ggccgcggtt
     1561 tctcattcca cgcctgacac tcagttccgg gaaggcagtt cgctccagga tggactgtat
     1621 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc aacttgagtc
     1681 caacccggaa agacacgact aatcgccatt ggcaacggcc attggcaaac aggatgtgcg
     1741 agagatgtct caagagttct tgaagtggcg gccttagtga ggctacactg aaaggacaaa
     1801 tttggtgaca tgtctcgcac aagactgtta ccacggttag gggatctgcc aggattacct
     1861 aaccttcgaa aaaccacctg ccagggtggt tttttcgttt acagggcaag agattacgcg
     1921 cggaaaaaaa ggatctcaag aagatccttt ataaattaga catccaacct cacaaagagg
     1981 aatacttaaa tcatcttggt atatgtatcg gccggattac atctcatata agcctcctcc
     2041 ccgcctgaca ccacactttg accatagcac ctcgcgccta actcgatata cagcccacca
     2101 tcaagcctct tttcacataa aaaatgacct atagttagat ggttagtgac caaaactgat
     2161 atggataaaa ggagctggct ttgaaaaaga tttttgtttc agtgttcgca gcggcagttg
     2221 cgctttcagc cctcaccggc tgcacgcgca caagctatgc catacacacc aatgacggtc
     2281 gcactattgt cagcgacggc aaacctaaag aatcagactc aggcctgctt ggctatacag
     2341 acgcgaatgg tgtcaaacag cagatcaata aaaccgacgt taaagaagtc tccgaaattc
     2401 cgcactgagc atcttctccg aaaaaggccg ctaatgtggc ctttttcttt acctgtggtt
     2461 ctccgccaaa atcccagcaa attgcatcac caaag
//
