LOCUS       NZ_CP083511              804 bp    DNA     linear   CON 08-JAN-2024
DEFINITION  Escherichia coli strain elppa5 plasmid unnamed8, complete sequence.
ACCESSION   NZ_CP083511
VERSION     NZ_CP083511.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21169128
            Assembly: GCF_020827635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Huang,Y.T.
  TITLE     Genome sequencing and analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 804)
  AUTHORS   Huang,Y.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) Department of Computer Science and
            Information Engineering, National Chung Cheng University, No.168,
            Sec. 1, University Rd., Min-Hsiung Town, Chiayi City 621, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 59x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:44:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,279
            CDSs (total)                      :: 5,157
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 79 of 219
            Pseudo Genes (incomplete)         :: 152 of 219
            Pseudo Genes (internal stop)      :: 42 of 219
            Pseudo Genes (multiple problems)  :: 50 of 219
            Pseudo Genes (short protein)      :: 4 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083511.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="elppa5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="Taiwan"
                     /lat_lon="24 N 120 E"
                     /collection_date="2019-11"
                     /collected_by="National Chung Cheng University"
     gene            complement(<1..155)
                     /locus_tag="K9U08_RS26280"
                     /old_locus_tag="K9U08_26295"
     CDS             complement(<1..155)
                     /locus_tag="K9U08_RS26280"
                     /old_locus_tag="K9U08_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_159053038.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVT"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(162..671)
                     /locus_tag="K9U08_RS26285"
                     /old_locus_tag="K9U08_26300"
     CDS             complement(162..671)
                     /locus_tag="K9U08_RS26285"
                     /old_locus_tag="K9U08_26300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
ORIGIN
        1 gtcacgcgct cgctcagcgc cgcgttctcc cgttgcataa gccctgacat tttccgccat
       61 tccccgaagg cgctctccca ctcgtccagc cttttcgagt agtcctgctg tagctgctct
      121 aatgcgctca gcaactgttt ttccagctcg gtcatcggca cttactcctt caccagtctc
      181 agcgcgttct gcttcgaccg cccgttcccg accgtccagc tctgcgtcct gtctacggcc
      241 gtccccttcg gcagcaccag aaaacgtccg ttgctgtctt ccaggtacgt cacgccccag
      301 gtcttcgcgt tcagcttcga cagcgctgcg ttctgccggt cgatttcggc cagatttgac
      361 gctatcaagc tcccctgata ccacaacacc ccgctcaggc tggcgaacag cagtcctgac
      421 accatcgtga tggtcagcca tgtccgcccg accatacgca tcacgctcag gcggttcgac
      481 tgcatggccg cgctcatacc ctgctcgtgg gcgcggatgg cggcgctgat tctcttcccg
      541 ctttcgctca gttccgctct gacagacttc tcgtgctcgc tgaatgcggc tttcagcatt
      601 tcgccggtac tctgctgctg cgctttcgat ttctgctcta agtccttcgc cagcgttaaa
      661 agactgttca tagatggctc ccttaagtcg gatgtttcgc cctccgtccg ggtcggcaat
      721 gctgatgctg ctctttgtgg tgcgcacgac ctcaaaacct gcgctttcca gcgcctcagt
      781 gacgtcctga cgcgttttaa gctc
//
