LOCUS       NZ_CP083510             2505 bp    DNA     linear   CON 08-JAN-2024
DEFINITION  Escherichia coli strain elppa5 plasmid unnamed7, complete sequence.
ACCESSION   NZ_CP083510
VERSION     NZ_CP083510.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21169128
            Assembly: GCF_020827635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Huang,Y.T.
  TITLE     Genome sequencing and analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Huang,Y.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) Department of Computer Science and
            Information Engineering, National Chung Cheng University, No.168,
            Sec. 1, University Rd., Min-Hsiung Town, Chiayi City 621, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 59x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:44:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,279
            CDSs (total)                      :: 5,157
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 79 of 219
            Pseudo Genes (incomplete)         :: 152 of 219
            Pseudo Genes (internal stop)      :: 42 of 219
            Pseudo Genes (multiple problems)  :: 50 of 219
            Pseudo Genes (short protein)      :: 4 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083510.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="elppa5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
                     /geo_loc_name="Taiwan"
                     /lat_lon="24 N 120 E"
                     /collection_date="2019-11"
                     /collected_by="National Chung Cheng University"
     gene            <1..254
                     /locus_tag="K9U08_RS26255"
                     /old_locus_tag="K9U08_26270"
     CDS             <1..254
                     /locus_tag="K9U08_RS26255"
                     /old_locus_tag="K9U08_26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228588631.1"
                     /translation="MSLSDLINQRLSGSLSVEERIGEKPEWMRNLDPYLANHFSNIELP
                     LPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFYDEIK"
                     /name="hypothetical protein"
     gene            280..825
                     /locus_tag="K9U08_RS26260"
                     /old_locus_tag="K9U08_26275"
     CDS             280..825
                     /locus_tag="K9U08_RS26260"
                     /old_locus_tag="K9U08_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            891..1241
                     /locus_tag="K9U08_RS26265"
                     /old_locus_tag="K9U08_26280"
     CDS             891..1241
                     /locus_tag="K9U08_RS26265"
                     /old_locus_tag="K9U08_26280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1311..1577)
                     /locus_tag="K9U08_RS26270"
                     /old_locus_tag="K9U08_26285"
     CDS             complement(1311..1577)
                     /locus_tag="K9U08_RS26270"
                     /old_locus_tag="K9U08_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1561..1785)
                     /locus_tag="K9U08_RS26275"
                     /old_locus_tag="K9U08_26290"
     CDS             complement(1561..1785)
                     /locus_tag="K9U08_RS26275"
                     /old_locus_tag="K9U08_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
ORIGIN
        1 aaatgagtct aagtgatctt attaaccaga gattatcagg ttcactttca gttgaagaac
       61 gcattggcga aaagcctgaa tggatgagaa accttgatcc ctatctcgcc aatcactttt
      121 caaatattga gttaccgctc ccttcaccat taaaagaata ccaagaaaaa atggatgaga
      181 tagattcaat tattaatgac atgatggttc taaaggaata tttcagagct aagttttacg
      241 acgaaataaa ataggcacgc tatacggagg cggagaatta tgttttttaa acggtattta
      301 acggagaagg aaatatacaa ggcagcactc ccaattgtta ggcttcaagg gagagttaac
      361 acttcattac gttttttatc caaatactca gatgtaaaca aaaatggatt aattgcattg
      421 catcttaatt tattacattt tatctcatac aacatacacg gtgtagagtc tctgaaaatc
      481 actcaagcag gcataaacaa agctgtacaa caaagccctc acgcagatga aataaaaaat
      541 attgcgcagg caataggacg caaaggacct gagtacatcg aaggattaat tagaaaccca
      601 tgggggaaga aaaatgataa tccactaata tttataatga gcattgaaga aagtataaaa
      661 gaaaccctta actcaggtta tatgttatat agagtagctc ccataactga gggtgaagca
      721 gctgctttca tggatgttct taaacaatta ttattatttg aggataattt tcgccttttt
      781 ggtagagact tggtttataa aatagaaaaa ttaaaaatca gataacaaag tatggtgaac
      841 gaaaaatgaa agagaggata gtgaaatgtg taataccaag attctcaact atgtattggt
      901 taattgcgtt cctagcattt atattccccg gtcgatcttt tgctgacggt tacaccgctt
      961 actaccgatg taatggtact caggaagcat ctcttactgt taaagataac aaactaagta
     1021 tggttgtagg tctttctaag tttaataggc aactggatac ttatagattc aaagaaaagg
     1081 gaatcgaagt agtaatgcag agagctgctt ctgatgataa ctctgaggcc ataatgtctt
     1141 ttattatgcc tgacgttaac aatatgacct tattagttaa aaataacggc tacattatcg
     1201 ttaagacaac ctgctggtta actaaaaccg acaaacagta gaacacgtag ttagtcgagt
     1261 atttaccata atacctgttg tcggcatcaa aaaagcccgc atagcgggct tcagtcgtaa
     1321 acttcgcggc gatgtccaat cgtggcggca aggataacaa gctcgtcatc tcggatctca
     1381 cacagaacgc gataatctcc cacccgatag cgccagaact cgcccagctc acctttgagc
     1441 ggcttccctg actggcgagg atcggcagca actgcaatgc gtaaactcat aaaatccaca
     1501 atccgtcgtg cgttctgttt gtccatcttg cgtaacgatt tgagcgcccg atcggaatag
     1561 ttaatcgtcc agaccaagtg acttcctcac gtcttcagac gaatgcactg cttcatcacc
     1621 acggcgaacg cgtgcagcag tttctgctga aagataataa tcctccaggt cttccagatg
     1681 cgccagtatt gcttcccgcg cataaaacgt ctttgttctg ccggtttgct tcgccagcga
     1741 gtccagacgg gactcaatct catcagataa ccggatagca agcatataaa cctcccgaac
     1801 accatttgct atacatgtat atcaacagta ccacaatcaa cctaaaagaa caaaaaataa
     1861 tcttgttagc ccctcttgat aatccgtaac gcggtcaata tatctagctt gagggctttc
     1921 ctcttaacta ttgaaaggat aacgattatg ggtcttaagc cgggtccgaa acgaattgcc
     1981 gattcaacag gggaacctga taaacgccag cgtgacaata aaaaaacgcc gggaaatact
     2041 gacaaattga aacccagcaa atcttcaaaa aaataagtca gtagttaaaa gccctccggg
     2101 gcttttttgt atctgccgtt cacgctgatt aaacataaca cccgttgccc gcaccgcccc
     2161 ggacgggttc gccgcgaacg gggtggcagt caggtaaaac cggcaacaaa ccacccgata
     2221 aaccgccccg ttcgcgacag ataaatttta cctggctgca tagccatgca gccaggtgaa
     2281 tgacatccgg tttcagcgta aatgcccgat gagaaccctg ttttcctgca tttcagccca
     2341 gcgacggacc gtcatagctt cgctgccggg aacgcgcttc cgtcagttcc ttttcgtgct
     2401 gccgttgctc gtgcgcccgt tctgcggcca cggccatctc atgacgctgc actaccggct
     2461 caaacgctgc agttgcccgc tcaagcgttc cacctgctga ctcaa
//
