LOCUS       NZ_CP083507            20432 bp    DNA     linear   CON 08-JAN-2024
DEFINITION  Escherichia coli strain elppa5 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP083507
VERSION     NZ_CP083507.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21169128
            Assembly: GCF_020827635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20432)
  AUTHORS   Huang,Y.T.
  TITLE     Genome sequencing and analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20432)
  AUTHORS   Huang,Y.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) Department of Computer Science and
            Information Engineering, National Chung Cheng University, No.168,
            Sec. 1, University Rd., Min-Hsiung Town, Chiayi City 621, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 59x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:44:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,279
            CDSs (total)                      :: 5,157
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 79 of 219
            Pseudo Genes (incomplete)         :: 152 of 219
            Pseudo Genes (internal stop)      :: 42 of 219
            Pseudo Genes (multiple problems)  :: 50 of 219
            Pseudo Genes (short protein)      :: 4 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083507.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="elppa5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Taiwan"
                     /lat_lon="24 N 120 E"
                     /collection_date="2019-11"
                     /collected_by="National Chung Cheng University"
     gene            complement(275..400)
                     /locus_tag="K9U08_RS25740"
                     /old_locus_tag="K9U08_25750"
     CDS             complement(275..400)
                     /locus_tag="K9U08_RS25740"
                     /old_locus_tag="K9U08_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="WP_001372321.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
                     /name="type I toxin-antitoxin system Hok family toxin"
     gene            complement(<342..491)
                     /gene="mok"
                     /locus_tag="K9U08_RS25745"
                     /old_locus_tag="K9U08_25755"
                     /pseudo=""
     CDS             complement(<342..491)
                     /gene="mok"
                     /locus_tag="K9U08_RS25745"
                     /old_locus_tag="K9U08_25755"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
                     /name="mok"
     gene            513..701
                     /locus_tag="K9U08_RS25750"
                     /old_locus_tag="K9U08_25760"
     CDS             513..701
                     /locus_tag="K9U08_RS25750"
                     /old_locus_tag="K9U08_25760"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001299721.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTTL
                     TFPENNPLIQTVHGKSV"
                     /name="hypothetical protein"
     gene            complement(670..1432)
                     /locus_tag="K9U08_RS25755"
                     /old_locus_tag="K9U08_25765"
                     /pseudo=""
     CDS             complement(670..1432)
                     /locus_tag="K9U08_RS25755"
                     /old_locus_tag="K9U08_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /name="plasmid SOS inhibition protein A"
     gene            complement(1429..1863)
                     /gene="psiB"
                     /locus_tag="K9U08_RS25760"
                     /old_locus_tag="K9U08_25770"
     CDS             complement(1429..1863)
                     /gene="psiB"
                     /locus_tag="K9U08_RS25760"
                     /old_locus_tag="K9U08_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="WP_000845901.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPAN
                     WMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVVQV
                     QRRFAPEAVSHSLALAASLDAQGYSVNDIIHILMAEGGQA"
                     /name="psiB"
     gene            complement(1918..3876)
                     /locus_tag="K9U08_RS25765"
                     /old_locus_tag="K9U08_25775"
     CDS             complement(1918..3876)
                     /locus_tag="K9U08_RS25765"
                     /old_locus_tag="K9U08_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="WP_001145469.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETALSALLAQTEEVSVPLDSLIK
                     SPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLAER
                     DIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAHIGDLL
                     GYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQSGWG
                     GKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRVALDAA
                     LLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEDERLNEL
                     MTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEIRAGSGVVVSWRYGNVCVQRGVQ
                     LRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPDKSLALLAW
                     TLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMALMAEKSRLAALLPEGWS
                     RDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAIGFHMRDWWQP
                     TKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKDNRWVPGWMCAP
                     HPQTDATERTDNLADAA"
                     /name="ParB/RepB/Spo0J family partition protein"
     gene            complement(3935..4168)
                     /locus_tag="K9U08_RS25770"
                     /old_locus_tag="K9U08_25780"
     CDS             complement(3935..4168)
                     /locus_tag="K9U08_RS25770"
                     /old_locus_tag="K9U08_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="WP_000006004.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDQGTHFRLVV
                     RQDGTLIWRSWNFEDCAGYWMNRYIRDFGILK"
                     /name="DUF905 domain-containing protein"
     gene            4533..5354
                     /locus_tag="K9U08_RS25775"
                     /old_locus_tag="K9U08_25785"
     CDS             4533..5354
                     /locus_tag="K9U08_RS25775"
                     /old_locus_tag="K9U08_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection system antitoxin AbiGi family
                     protein"
                     /protein_id="WP_001272932.1"
                     /translation="MSAIENQETVIQAVNSLHPTTLFHFTKNEEAFYSILTELYFKPFL
                     ARERIVGVKGRRNFAVPMVSFCDIKLSQIKDHSGKYGEFGFGLTKEWAEKNDLHPVLYM
                     NQGSELFSKYNKRIRKLKDALIPLWSKRHILDNKERKYFESLKEEYADLYNLLRYMKNY
                     KGKLERTNEKTIENYIYADEKEWRYVPHPFVGDLWPSLNLERVVEPNQKAVLSKKFSEF
                     GIGFSFDDIKYILIPDDSHVSNLINCLMSIRNYDPYIISKVLTMDKVKQDF"
                     /name="abortive infection system antitoxin AbiGi family
                     protein"
     gene            complement(5893..10656)
                     /gene="avs4"
                     /locus_tag="K9U08_RS25780"
                     /old_locus_tag="K9U08_25790"
     CDS             complement(5893..10656)
                     /gene="avs4"
                     /locus_tag="K9U08_RS25780"
                     /old_locus_tag="K9U08_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AVAST type 4 anti-phage nuclease Avs4"
                     /protein_id="WP_000240574.1"
                     /translation="MVKPNWDNFKAKFSENPQGNFEWFCYLLFCQEFKMPAGIFRYKNQ
                     SGIETNPITKDNEIIGWQSKFYDTKLSDNKADLIEMIEKSKKAYPGLSKIIFYTNQEWG
                     QGRKSHEPEGDKNADNYLETVGNSNDPKIKIEVDQKAYESGIEIVWRVASFFESPFVIV
                     ENEKIAKHFFSLNESIFDLLEEKRKHTENVLYEIQTNIEFKDRSIEIDRRHCIELLHEN
                     LVQKKIVIVSGEGGVGKTAVIKKIYEAEKQYTPFYVFKASEFKKDSINELFGAHGLDDF
                     SNAHQDELRKVIVVDSAEKLLELTNIDPFKEFLTVLIKDKWQVVFTTRNNYLADLNYAF
                     IDIYNITPENLVIKNLERGELIELSDNNGFSLPQDVRLLELIKNPFYLSEYLRFYTGES
                     IDYVSFKEKLWNKIIVKNKPSREQCFLATAFQRASEGQFFVSPACDTGILDELVKDGIV
                     GYEAAGYFITHDIYEEWALEKKISVDYIRKANNNEFFEKIGESLPVRRSFRNWISERLL
                     LDDQSIKPFIAEIVCGEGISNFWKDELWVAVLLSDNSSIFFNYFKRYLLSSDQNLLKRL
                     TFLLRLACKDVDYDLLKQLGVSNSDLLSIKYVLTKPKGTGWQSVIQFIYENLDEIGIRN
                     INFILPVIQEWNQRNKVGETTRLSSLIALKYYQWTVDEDVYLSGRDNEKNILHTILHGA
                     AMIKPEMEEVLVKVLKNRWNEHGTPYFDLMTLILTDLDSYPVWASLPEYVLQLADLFWY
                     RPLKETGERYHSMDIEDEFGLFRSHHDYYPESPYQTPIYWLLQSQFKKTIDFILDFTNK
                     TTICFAHSHFAKNEIEEVDVFIEEGKFIKQYICNRLWCSYRGTQVSTYLLSSIHMALEK
                     FFLENFKNADSKVLESWLLFLLRNTKSASISAVVTSIVLAFPEKTFNVAKVLFQTKDFF
                     RFDMNRMVLDRTHKSSLISLRDGFGGTDYRNSLHEEDRIKACDDVHRNTYLENLALHYQ
                     IFRSENVTEKDVIERQQVLWGIFDKYYNQLPDEAQETEADKTWRLCLARMDRRKMKITT
                     KEKDEGIEISFNPEIDPKLKQYSEEAIKKNSEHMKYVTLKLWASYKREKDERYKNYGMY
                     EDNPQIALQETKEIIKKLNEEGGEDFRLLNGNIPADVCSVLLLDYFNQLNNEEREYCKD
                     IVLAYSQLPLKEGYNYQVQDGTTSAISALPVIYHNYPMERETIKTILLLTLFNDHSIGM
                     AGGRYSVFPSMVIHKLWLDYFDDMQSLLFGFLILKPKYVILSRKIIHESYRQVDYDIKK
                     ININKVFLNNYKHCISNVIDNKISIDDLGSIDIVDLHILNTAFQLIPVDTVNIEHKQLV
                     SLIVKRFSTSLLSSVREDRVDYALRQSFLERFAYFTLHAPVSDIPDYIKPFLDGFNGSE
                     PISELFKKFILVEDRLNTYAKFWKVWDLFFDKVVTLCKDGDRYWYVDKIIKSYLFAESP
                     WKENSNGWHTFKDSNSQFFCDVSRTMGHCPSTLYSLAKSLNNIASCYLNQGITWLSEML
                     SVNKKLWEKKLENDTVYFLECLVRRYINTERERIRRTKQLKEEVLVILDFLVEKGSVVG
                     YMSRENIL"
                     /name="avs4"
     gene            complement(10732..11151)
                     /locus_tag="K9U08_RS25785"
                     /old_locus_tag="K9U08_25795"
     CDS             complement(10732..11151)
                     /locus_tag="K9U08_RS25785"
                     /old_locus_tag="K9U08_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001062122.1"
                     /translation="MAKHLDNRDINAIVNLIRGWKEPKLSWSAICEAAEPLVGKLPSRQ
                     SLNAHEAIVTAYQTKKEALKGRGPKNPRPASLNIAAARIVNLESEIEELKEENRRYKQQ
                     FVIWQYNAYKYGMTEHQLNAQLTKIDRERSDGERR"
                     /name="hypothetical protein"
     gene            complement(11144..>12046)
                     /locus_tag="K9U08_RS25790"
                     /old_locus_tag="K9U08_25800"
                     /pseudo=""
     CDS             complement(11144..>12046)
                     /locus_tag="K9U08_RS25790"
                     /old_locus_tag="K9U08_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697682.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /name="integrase"
     gene            12101..13314
                     /locus_tag="K9U08_RS25795"
                     /old_locus_tag="K9U08_25805"
     CDS             join(12101..12392,12392..13314)
                     /locus_tag="K9U08_RS25795"
                     /old_locus_tag="K9U08_25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_164523141.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQGEYDSQWAAICSIAPKIGCTPE
                     TLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFGEGGVRP
                     PLEKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEI
                     QRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAV
                     AAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSSMETTF
                     VLDALEQALWARQPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAES
                     INGLYKAEVIHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGN
                     DDLAA"
                     /name="IS3 family transposase"
     misc_feature    12382..12498
                     /locus_tag="K9U08_RS25795"
                     /old_locus_tag="K9U08_25805"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            <13358..14445
                     /locus_tag="K9U08_RS25800"
                     /old_locus_tag="K9U08_25810"
                     /pseudo=""
     CDS             <13358..14445
                     /locus_tag="K9U08_RS25800"
                     /old_locus_tag="K9U08_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     misc_feature    13513..13629
                     /locus_tag="K9U08_RS25800"
                     /old_locus_tag="K9U08_25810"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo=""
                     /db_xref="RFAM:RF01497"
     gene            complement(<14488..>15576)
                     /locus_tag="K9U08_RS25805"
                     /old_locus_tag="K9U08_25815"
                     /pseudo=""
     CDS             complement(<14488..>15576)
                     /locus_tag="K9U08_RS25805"
                     /old_locus_tag="K9U08_25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697682.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /name="integrase"
     gene            complement(15616..16778)
                     /locus_tag="K9U08_RS25810"
                     /old_locus_tag="K9U08_25820"
     CDS             complement(join(15616..16538,16538..16778))
                     /locus_tag="K9U08_RS25810"
                     /old_locus_tag="K9U08_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611812.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_257695600.1"
                     /translation="MVLESQSEYDSQWATICSIAPKIGCTPETLRVWVRQHERDTGGGD
                     GGLTTAERQRLKELERENRELRRSNDILRQASAYFGEGGVRPPLEKVMPLLDKLRKLYG
                     VGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEIQRVYDENHKVYGVRKVW
                     RQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQ
                     LWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGT
                     VHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKN
                     RAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
                     /name="IS3 family transposase"
     misc_feature    complement(16432..16548)
                     /locus_tag="K9U08_RS25810"
                     /old_locus_tag="K9U08_25820"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(16952..18490)
                     /locus_tag="K9U08_RS25815"
                     /old_locus_tag="K9U08_25825"
     CDS             complement(16952..18490)
                     /locus_tag="K9U08_RS25815"
                     /old_locus_tag="K9U08_25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_013362830.1"
                     /translation="MTMNNLLLGLVPERIRARANGYSFLLYDTSWTLDRNITINLADIH
                     HYLSDGTLDGCLRTLAFYASNRSSSHTKNILERFQHMLRETDAREITDTVLINYRSMLS
                     PATEWYLATIRGFLRSWHELGYPGISKEVNRLLDGWTLKGNRKGDAVKRMDPQEGPLTD
                     NELTAFNEGAVRAYEKSLITIAELAIALGTSHTGRRPIQISLLRVVDVLCGKNGKGEPF
                     YVLNVPRAKQRSSSFRINFKPFAISLELWAILSAQARNAIAQVENRLGFELQEDDRQQI
                     PLFPDLDVLDAVQSPYEFCQLHATDKLHIPAAEITDTLQYIVEKSDIRSERTGELLHIN
                     ARRFRYTTGTRAAREGFGELVIAELLDHADTQNAGVYIKNIPEHVKKLDEAVGFQLAPY
                     AQAFVGALVDSERDAHRGNDPTSRIRTEMGQGVGTCGEHGFCGANVPIPCYTCMHFQPW
                     LDGPHEDVYQGLLNERERVKEITGDIQIAAVLDRSIIALADVIMRCAKRREELS"
                     /name="site-specific integrase"
     gene            complement(18477..19724)
                     /locus_tag="K9U08_RS25820"
                     /old_locus_tag="K9U08_25830"
     CDS             complement(18477..19724)
                     /locus_tag="K9U08_RS25820"
                     /old_locus_tag="K9U08_25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414651.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_000414651.1"
                     /translation="MEKNYRVTTFVMDSGERYCMVVDRASGLPMYYPTLFLTTQIRNRE
                     DAFSTMLAAANNLVMLLRFVESRDIDLEQRFLTKDFFKPHELDDLRDFAQCKQRKGPLT
                     VSSTSWLADAMTDIVDNGTQHSRLTTFANYLHWYAMHILKTTELEVVEKINAMAAQIKT
                     RRPSKKRRNHDLQDRSLSDVQLDALFEVIQPRSELNPFSIDVQRRNRLMILLLFYLGIR
                     GGELLNIRIQDIDFSANRIRIVRRADEQADSRTNEPNAKTRERLLPLAESLAQELHSYI
                     LQDRRKVRNAKKNDYLFVTYKSGPTLGNPISKGGYHKIFSVVRSISPQLYAATGHSLRH
                     TWNRKFSERMDAMNEQVSEERQEQLRSYLMGWRDGSGTAATYNKRFIRQKGFEAALALQ
                     AGNGTSLPEDFKDDHE"
                     /name="site-specific integrase"
     gene            complement(19884..20411)
                     /locus_tag="K9U08_RS25825"
                     /old_locus_tag="K9U08_25835"
     CDS             complement(19884..20411)
                     /locus_tag="K9U08_RS25825"
                     /old_locus_tag="K9U08_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="WP_000290793.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDKQ
                     TGEIREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEILVK
                     TTGTMQMLGRAAGTQTQPEEAQQFSGQPQPESQPEPKKGGAKTKGRGRKAAQPEPQQPS
                     EPAYDFDDDIPF"
                     /name="single-stranded DNA-binding protein"
ORIGIN
        1 caggaagaaa aagagaataa tcacagggaa ggtgatgtga tttctgacag gccataacca
       61 gagaaggcgg gggaaaagaa cgggcgccgc aggcgcgcgt tcatccccgt tcctgaaggg
      121 aaacgacctt cctgaagtca ggttgttcat gcccgtacaa catcagcaag gagaaagggg
      181 ctaccggcga accagcagcc cctttataaa ggcgcttcag tagtcagacc agcatcagtc
      241 ctgaaaaggc gggcctgcgc ccgcctccag gttgctactt accggattcg taagccatga
      301 aagccgccac ctccctgtgt ccgtctctgt aacgaatctc gcacagcgat tttcgtgtca
      361 gataagtgaa tatcaacagt gtgagacaca cgatcaacac acaccagaca agggaacttc
      421 gtggtagttt catggccttc ttctccttgc gcaaagcgcg gtaagaggct atcctgatgt
      481 ggactagaca tagggatgcc tcgtggtggt taatgaaaat taacttacta cggggctatc
      541 ttctttctgc cacacaacac ggcaacaaac caccttcacg tcatgaggca gaaagcctca
      601 agcgccgggc acatcatagc ccatatacct gcacgctgac cacactcact ttccctgaaa
      661 ataatccgct cattcagacc gttcacggga aatccgtgtg attgttgccg catcacgctg
      721 cctcccggag tttgttcggc accatccacc gctccatctc acgaacagca tgaccgacca
      781 cccttgccgc cataccagcc tctgcgatga cctgccagaa tggcatatcc tgccactgcc
      841 agcgttccat tccggccagt gacggaaaac gctcgcccca ctgccagaac agggtttcca
      901 ggtcatgctc tgccacccgg tcactccagc gtgcatgcca gcttccgact gtttccggcg
      961 aatggaatgc cagttcgatt tcagcctgag cgaccagcgc ctggtacgca cgggctttct
     1021 gctctgcctc cctcgcctgc tggcggctgt acttctgcat gaccagcgcg gatttatggc
     1081 gactgcgttc cctgacacga aaccgcaccg acgggaacaa gcgctctgct gcatcaccgg
     1141 gaaggggaag gaggcagacc tcgccctgtg tttcaatcag cacatcgatg gcatacagcc
     1201 actgaagacg gttgccgtgc agttcttctg cggtcaggga cggcatgaaa aatcgcaggt
     1261 cagacagcga tatcctgcgg cttccgttca gacagcggaa aaacgcacac gcatacggat
     1321 attcagccag cgtgttaccc tgatggcggc gtttttcggt ctccgccacc gcccgccatg
     1381 cggcctgttg ctctgtgctg agaggaacca gtgcctgtga acgtgcgctc atgcctgacc
     1441 tccttccgcc atcaggatat gaatgatgtc gttaacgctg tacccctgcg catccagtga
     1501 cgccgccagt gccagtgaat ggctgacagc ttccggtgca aaccgccgct gtacctgaac
     1561 caccgcaaac ggctcaccac cggcattcac cagaaccagc acccagacct gagagacgtc
     1621 tcccggcgaa cataatgcca ggtggaaacg ttcgtgggct ggcgtgaaac gaacctggac
     1681 ggggaaaaag cccccgaact cactgccata ctcgccattc atcatccagt tagccggtgc
     1741 gtccacttca cgcatcacct cgtgagtgag attacggcgc atatcgcttc ccgcagcacg
     1801 gatatcttca tattcctgtg cgttcatgga ctgcagggca ttcagggtca gttcagtttt
     1861 catcacggta tctcccttgc tgccgccccg tctccggcgg ggcggtgtgg ttgttcatca
     1921 ggcggcatca gccaggttat cggtgcgttc agtggcatct gtctgtggat gtggtgcaca
     1981 catccagcct ggaacccagc ggttgtcttt catatggtgc tctgcatgtt cagccgcatc
     2041 acctttcttc atcttctccg cgtcccgtgc ggcaccggac agtccggcct cattcagggc
     2101 tgcgataatc tgcggctttt tcaggtgtcc gaagaagttt gcttttgtcg gctgccacca
     2161 gtcgcgcatg tggaatccga tagcgctttc cagcgagtca agcggactgc gtgacgtgtg
     2221 accacactca cgggtctgga caccgttaag gctgcatgcg gtgcagaaac tgagcaggga
     2281 taacagcacc tcctgactga gggacaggaa tgtcgtcatg tcccgtgacc atccctccgg
     2341 cagcagggct gcaagacggg atttttctgc catcagcgcc atgaatgcgg caccttcctt
     2401 ccccgatggc gcatcgctgg tcagcgaata atgttcacat tccaggctga tttgtgctgg
     2461 tttactgtac gccccgctgc caaacacatt caggcagagc gtccatgcca gcagtgccag
     2521 agatttgtcc ggctgctgca tgagtgccgc ctggactgcc agcgtgcgct ctgaggacat
     2581 tttcgtcagc aacggcaggc tgatttcctc cactgacgct ttctcctgca cctgttccgt
     2641 gcggtcagcg tcgtcagtcg cgtcatcttc actgcgcaac tgcacaccac gctggacaca
     2701 tacgttgcca taacgccagg acaccaccac accgcttccg gcacgtatct ccggcgtcca
     2761 cgctctgacc ttcgccatgc agcgcatcag cttcatttct gcttccagca ggtcggattc
     2821 ctcacactgg ttttccagcg cgtcgtaacg cgtcatcagt tcgttcaggc gttcgtcttc
     2881 cgcctccgtc agcaccgctt ccggctccgg cagacagcgg tatgttccgg catcctcacg
     2941 gcactcaccg acaggctcca tgcgcccggc gcaccattcc cagccttcgg cttcccgaag
     3001 gtgttcagcg acagcctgga gtttttccag cagggcggca tcgagcgcca cgcggtcgac
     3061 ataaccgtcc ccgtcatcgc tgaacaaatc ggtgcgcagt tcgtctggcg agaaggcatc
     3121 agcccccacg aagcggaatt tactgttccc agccaccgcc acttcacttt cggtcaccag
     3181 acgacgaatg gtctgtactt ccggtttacc gccccatccc gactgacagg cggcttcaaa
     3241 cacctgcacc tgacgcgcgg tgtcgttctc cagcgccagc gcctgacagt gttcggtggt
     3301 gatgcggtct tctgccagcg catcgaggat gacaggcgca aggtcagcca gtttcagcat
     3361 tcgctgaacg tggcggggtg aatagcccag caaatcaccg atgtgtgcag gtgttttgcc
     3421 ttcctgcgcc attgcgcgga atccggcaat ctgttcggca gggtgcatat cccgacgatg
     3481 accgttctcg gtcattgatg cggcagtcgc cagctcctgc ggaataattt ttacgcggac
     3541 aggccagtca gccggaatga tgtcacgctc tgccagcatg ttgagtgctg ccagtcggcg
     3601 accacctgcg gcgacaccgt aacggtcacc tggcagggca tgaacaacca gattctgcag
     3661 caggccgact cccttaatgg aatcagccag ttcactgacg gactccgcag aatacggcac
     3721 cgtgcgcaca ttcagcggtg atttaatcag tgaatccagc ggcacgctca cttcctccgt
     3781 ctgcgccagc agggcagaca gtgcagtttc ctgcgttttt acagatttac ggcttgcttt
     3841 gcgggtggtt tctggttcac acttcgttac aggcataatc atccccttat aattgcttat
     3901 gtcctgccgg acgttcgcgc gtccggcacc tctcttactt aagaatcccg aaatccctga
     3961 tgtaccggtt catccagtac ccggcacagt cctcaaaatt ccaggagcgc cagatcaacg
     4021 tgccatcctg acggacaacc aggcgaaaat gtgttccctg atcatcctcg ataaagacat
     4081 tccggtactg tgcggcaacg gcttcggcct gtttgcgggt aaaaggaccg tccggcagtc
     4141 cctgaagtat tctgaaatat tcgctcatcc tcatctctcc ggtgatgccc cgcactggac
     4201 ggggcggttg tcactgtctt tcgttagaac ggttcacata acctgatact tataaatcgt
     4261 gaaaaaaaac cttttttatc agaaactaag gcataagaaa tgatttaact gtaaagaatt
     4321 tagttaaaat ataatctatc aaagtgcata attttacttt ttttggtgac ccagctcgga
     4381 cctttgattc aatctggcat agattttggt aagagatcaa aaaaccggaa atctcaaaaa
     4441 gtgcgtggaa ttaaaccaag tgtgcttaca gtacaaagac tagtatgata acctttgcta
     4501 agtcaggttt tacactatgg aacagttaat atatgagtgc tatagaaaac caagaaacag
     4561 ttatacaagc agttaatagt ttacatccaa caacgttgtt tcactttact aaaaatgaag
     4621 aggcattcta tagcatttta acagaactct attttaaacc atttcttgcc cgtgaacgca
     4681 tagtcggagt aaaaggaaga agaaatttcg cagtaccaat ggtttcattc tgtgacatca
     4741 agctttctca gattaaagat catagtggaa aatatggtga atttggattt gggttaacaa
     4801 aagaatgggc tgagaaaaat gatctccatc ccgttctata catgaatcaa ggcagtgaat
     4861 tattttcaaa gtataataaa agaattagaa aactaaagga tgcgttaatt ccgctatggt
     4921 cgaaaaggca tatccttgat aataaagagc gtaagtattt tgaaagcctt aaagaagagt
     4981 acgctgacct ttataattta ttacgctata tgaaaaatta taaagggaaa ttagaaagga
     5041 cgaacgaaaa aaccattgag aattatatct acgctgacga aaaagaatgg agatatgtac
     5101 cgcatccgtt tgttggcgat ttatggccta gcctaaattt agagcgagtg gtggaaccta
     5161 accagaaggc cgtcttaagt aaaaaattct ctgaatttgg cattggtttt tctttcgatg
     5221 atattaaata tatacttatt cctgatgata gccacgtttc caatttgata aattgcctta
     5281 tgagtataag aaattatgat ccttatataa taagtaaagt tttaacgatg gataaagtaa
     5341 aacaggattt ctaacacttc cacatagaaa ttccattaat aaacaaaaaa ttagctttat
     5401 tgaggacagc tttacctaaa gctgaaaagt gctaaatatc aaactttcat cccataagct
     5461 atgatatagc actcacttta ttgataaatt ccgaggtatt catgaaaggt cagatcgata
     5521 tcgaactcca aagattgata cgggcgcggt ataacaccgc ggtagaaaat tggagtgaat
     5581 ggaaatggtt agaaatcggg catggcgtcg tcatcatata tctcggttaa aatctaagag
     5641 aaaaacatat cgaattgcgt tggctaaaac tgaaaaagtt gtagggaaaa tttactgcac
     5701 cccttgtgct tgttcttgtt atttatgtgg taatcaaaga atttatcacg gagtgaacat
     5761 tcaagagcaa cgagctttgc tgcgttttac tgattgactt ataagatatt aatcttttcg
     5821 ataataaaaa ggctccaatc cctggagcct aaattggaaa agctctcatt aaaattatta
     5881 ttttcaacta catcacagaa tattttcccg tgacatataa ccaacaacag atcctttctc
     5941 taccaaaaaa tccaatatta ctaagacctc ttctttcaac tgtttggttc gtctaattcg
     6001 ctcacgctca gtgttaatat accgcctaac caaacattct aaaaaataaa cagtatcatt
     6061 ttccaatttc ttttcccata gctttttatt aaccgacaac atttctgaaa gccaggttat
     6121 accttgatta agatagcaac tggcaatgtt attcaaagat ttggcaagag aatataaagt
     6181 tgaagggcaa tggcccatag tcctagatac atcgcaaaag aattgactat tgctatcttt
     6241 aaatgtgtgc caaccattag agttttcttt ccatggagat tcagcaaaaa ggtaactttt
     6301 tataatttta tctacatacc aatacctatc tccatccttg cacaaagtaa ccactttatc
     6361 aaaaaacaaa tcccaaacct tccaaaattt ggcgtaagta tttaatctat cttcgacgag
     6421 aataaatttt ttaaataact ctgaaatagg ctctgaaccg ttgaaaccat caagaaaagg
     6481 ttttatataa tcgggaatat cgctcacggg cgcatgaagc gtaaagtagg caaatctttc
     6541 caagaaagac tgccgaagag cgtaatcaac tctatcttct cgaacacttg acaataggct
     6601 tgtagaaaat cttttaacaa ttaaggaaac caattgctta tgttcaatat taacagtatc
     6661 aactggaatt aattggaaag ctgtgttcaa aatatgtaga tcaactatat ctatacttcc
     6721 caaatcatct atagatattt tattatcgat gacatttgat atgcaatgct tatagttatt
     6781 taaaaacacc ttattaatat ttattttttt aatgtcatag tctacttgac gataactttc
     6841 atgaatgatt tttcttgaaa gaattacata ctttggcttt aaaatcaaaa aaccaaacaa
     6901 tagagactgc atatcatcaa aatagtctag ccataattta tgaatcacca tactaggaaa
     6961 tactgagtag cgcccacctg ccattccaat agagtggtca ttaaacagtg tcaaaagtaa
     7021 tattgttttt atagtctccc tttccattgg ataattatga taaatcacgg gtaaggctga
     7081 aattgccgag gttgtcccat cttgtacctg ataattatag ccttccttca acggaagttg
     7141 agaatatgct agaacaatat ctttacaata ttctctctct tcattattca actgattaaa
     7201 gtaatctaac agtaatacag aacaaacgtc tgctggtata ttaccattta atagtctgaa
     7261 atcttcaccc ccttcctcat taagcttttt tattatttct ttggtctctt gtaaagcaat
     7321 ttgcggattg tcctcataca ttccataatt cttataacgt tcatcctttt ctcttttata
     7381 gcttgcccat agtttcagcg ttacatactt catatgctcg gagtttttct ttattgcttc
     7441 ctcactatat tgctttagtt tagggtcaat ctcaggattg aatgatatct caatcccttc
     7501 atctttctcc ttggtagtta ttttcatctt tcgccgatcc attcttgcca agcagagcct
     7561 ccacgtctta tcggcttcag tttcttgagc ttcatctgga agctgattat agtatttgtc
     7621 gaaaataccc cagagcactt gttgcctttc aatgacatct ttctccgtta cattttcact
     7681 cctgaaaatt tgataatgca aggcaagatt ttcaagataa gtatttctat gcacatcatc
     7741 gcaagcttta attctatctt cttcgtgcaa agagtttctg taatctgtac cgccaaagcc
     7801 atccctgagg gagattaatg aacttttatg tgttctgtct agaaccattc gattcatatc
     7861 aaaacggaag aaatcctttg tttggaatag tactttagct acattgaatg tcttctcagg
     7921 gaatgcaagt acaatactcg taactactgc agaaatagaa gctgacttgg tatttcttaa
     7981 caagaaaaga agccaacttt ccaacacttt cgagtctgca tttttaaaat tctcaagaaa
     8041 aaatttttcc aatgccatat gaattgatga aagtaagtag gtagagacct gtgttcctcg
     8101 atatgagcac cacagacgat tgcatatata ttgctttata aactttcctt cttcaataaa
     8161 gacatcgact tcttcaattt cgtttttagc aaaatgggag tgggcaaaac atatcgttgt
     8221 cttgttcgta aaatcaagaa taaagtctat tgtttttttg aactgtgatt gtagtaacca
     8281 atatatagga gtctgatatg gactttctgg ataatagtcg tgatgagacc taaatagacc
     8341 gaactcatct tcaatatcca tactgtgata acgttcgcct gtttctttaa gtggccgata
     8401 ccagaacaga tctgccaatt gtagaacata ttccgggaga gatgcccaaa ccggatatga
     8461 atctaagtca gtaaggatta aggtcataag gtcgaaatat ggggtaccgt gctcgttcca
     8521 cctattttta agaaccttaa ctaaaacctc ttccatttca ggtttaatca tggccgcccc
     8581 atgaagaatc gtatgcagaa tatttttctc attatccctt ccggataaat agacatcctc
     8641 atctacagtc cattgataat attttagggc tatcaaacta gataatcgag tcgtttcacc
     8701 cactttgttt ctttgattcc actcctgaat cacaggaagt ataaaattaa tatttctgat
     8761 ccctatttca tctaaatttt catagataaa ttggatcaca ctctgccaac cagttccctt
     8821 aggcttagta agaacatatt taatggaaag cagatctgaa ttacttacac ctaactgttt
     8881 aagcagatcg taatcaacgt ccttgcaagc aagcctcaat aagaaagtaa gtctttttaa
     8941 tagattctgg tcactactaa gtaaatatct tttaaagtaa ttaaaaaata tgcttgaatt
     9001 atcggaaaga aggacagcta cccataactc gtctttccaa aaatttgata ttccttctcc
     9061 acagactatt tctgcgataa aaggctttat ggactggtca tctaaaagca atcgttcaga
     9121 tatccaattc cgaaaactac ggcgaacagg aagtgattct cctatttttt cgaagaactc
     9181 gttattgttc gctttacgga tataatcgac agaaattttc ttttctaatg cccattcctc
     9241 gtatatatca tgtgtaatga agtaaccagc agcttcatag ccgacaattc cgtctttaac
     9301 taactcatct aaaattccag tatcacatgc cggggagaca aaaaattggc cctcactagc
     9361 ccgctgaaaa gcagtcgcta agaaacactg ctcccgagaa ggtttatttt tgacaataat
     9421 cttattccaa agtttttctt tgaagctcac ataatcgatg ctttcaccgg tatagaacct
     9481 caaatattca cttagataaa atggattttt gattagttct aataatcgaa catcttgagg
     9541 aaggctaaat ccattgttat cagataactc tattagctcg ccgcgttcaa ggttctttat
     9601 tactaagttt tcaggagtta tattataaat atctatgaaa gcatagttca gatctgccaa
     9661 gtagttgtta cgggttgtga aaacaacctg ccatttatcc tttattaaaa cagtcaggaa
     9721 ttctttaaaa ggatcgatat tggtcagttc taaaagcttt tcagcagaat ctacgactat
     9781 gaccttacgt aattcgtctt gatgagcatt agagaaatcg tctaagccat gcgcaccgaa
     9841 tagctcatta atactgtcct ttttaaactc gctagcctta aagacataga aaggagtgta
     9901 ttgtttttct gcttcataaa tttttttgat aactgctgtt tttccaaccc caccttctcc
     9961 gctgacgatg acaatttttt tctgaactag attctcatgt agaagttcta tacaatgtcg
    10021 tctgtcaatt tcaatacttc tgtctttgaa ctctatattg gtttgaattt catataaaac
    10081 attttctgtg tgcttgcgct tttcttctaa taaatcaaag atgctttcat taagggagaa
    10141 gaaatgttta gcaatctttt cattctcaac tattacaaac ggtgattcaa aaaaactagc
    10201 aactctccat actatttcga tacccgactc atatgctttc tgatcaactt caatttttat
    10261 tttgggatcg ttactatttc cgacagtttc caaataatta tcagcgttct tatcgccttc
    10321 aggttcatgg gactttctcc cctgccccca ctcttgatta gtatagaaaa tgattttact
    10381 taatcctgga taagcctttt tgcttttctc aatcatttct ataagatcag ctttgttatc
    10441 cgacaatttt gtgtcataga atttagattg ccaaccgata atttcattat ctttggttat
    10501 tggattagtt tcgataccag attgattctt atatctaaat atacctgcgg gcattttgaa
    10561 ttcttgacag aacaacaagt agcaaaacca ctcaaaatta ccttgaggat tctcactaaa
    10621 tttagcttta aagttatccc agtttggttt gaccatttaa ttattctcct atttttttgt
    10681 aaataactac ccctacacag agaaatgtac cctcgatatc gtactcagca actatcttct
    10741 ttctccatca gagcgctcac ggtcaatctt tgtcagctgc gcattaagtt gatgttctgt
    10801 cattccatat ttgtatgcat tgtattgcca gatcacaaac tgttgtttgt acctccggtt
    10861 ctcttcctta agctcttcga tttcgctttc aagattcaca atcctcgcgg ctgcaatgtt
    10921 caaactagct ggacgcggat ttttggggcc acggccttta agcgcctctt tctttgtttg
    10981 ataggcagtc actattgcct catgtgcatt aagggactgt cttgacggca actttccgac
    11041 caatggttct gccgcctcac aaattgctga ccaggacagt tttggttcct tccatcctcg
    11101 aattagattt acgatggcat taatgtctct attgtccaga tgcttagcca aggccacctc
    11161 ctaacaaagt tgtcaaatca tctaacatca cagcttcttc actttctttg cctttttgct
    11221 cgatggccac aatagacgtc tttgcgacaa cacggcgaag ctggctgaaa tcattgcccc
    11281 ggagcttaat ctggacacca ttttcaatgt ctggattctc taaaatagcc actagttcac
    11341 gaagccgggt aacagttttc ttgtggtact gataccagcg atcagttcct atctcaccac
    11401 tttccactgc tgcgtcagca agaaaaaaca gtcgctctaa tttctccaga cgtatcttaa
    11461 tgcgatccaa cttttcagca tcatcgcctt tgatgcaaac ctgttcggta caattgacgc
    11521 aatctcggaa tttctcacaa ggactcatgg tgtaatcatg cacacagtag ccatactccg
    11581 tcacatggac tgcggcatgc tctaaggtat tgaactcctg catagtcacg ggcagatgct
    11641 ttgccacctc gccagcaggc ccaaatagcg ctgttgtggg atcaaggcgt tcagcaatgc
    11701 caaccagttc gtattcagtc atgtggttgt aagttcgatt ctgtttcaca tctgcccgcc
    11761 ccgaccactt ggcaatttcc aagtttgaca ggccaccgcg ctggataatg gtgttgagca
    11821 ggtgtcgagc ctgatgtgag gttaatttca ccggctctcc gtttacagca tggtaaccat
    11881 ggcgatcaaa gatactggca tgttttcctt tcaaggacag acgaggcgtc agatcactgt
    11941 tgaagaaatt attggttggc ttatgtaact caactggtat gcatcctctg ttcccgtgga
    12001 actgatttac gttcagtaca aacaaggcat tgctgtacct aattcctgaa ccgccccggg
    12061 aatcctggag actaaactcc ctgagaaaga ggtaaacagg atgactaaaa atactcgttt
    12121 ttcccccgaa gtccgtcaga gggcgattcg tatggttctg gaaagtcagg gcgaatatga
    12181 ctcacagtgg gcggcaattt gttccattgc cccaaagatt ggctgtacac cggagactct
    12241 gcgtgtctgg gtacgccagc atgagcggga taccggaggc ggtgatggcg ggctcaccac
    12301 cgctgaacgt cagcgtctga aagagctgga acgtgaaaat cgtgaactgc gccgcagtaa
    12361 cgatatcctt cgccaggctt ccgcttattt tgcgaaggcg gagttcgacc gcctctggaa
    12421 aaaatgatgc cactgctgga taagctgcgt gagcagtacg gggtcggacc gctatgcagc
    12481 gaactgcata ttgccccgtc aacgtattac cactgtcagc aacagcgaca tcatccggat
    12541 aaacgcagtg cccgtgcgca gcgcgatgac tggctgaaga aagagataca gcgcgtatac
    12601 gatgaaaatc atcaggtgta cggtgtgcgt aaagtctggc gtcagttgtt acgggaaggt
    12661 atcagagtgg ccagatgcac tgtggcacgt ctcatggcgg ttatgggact tgccggtgtt
    12721 ctccggggta aaaaggtccg tacgaccatc agccggaaag ccgttgccgc aggcgaccgc
    12781 gtaaaccgtc agttcgtggc agaacgacct gaccagctgt gggtggctga ttttacttac
    12841 gtcagcacat ggcggggctt cgtctatgtg gcgttcatca ttgatgtgtt tgccggatac
    12901 atcgtggggt ggcgggtctc atcgtctatg gaaacgacat tcgtgctgga tgctctggag
    12961 caggcgttat gggcccgtca accgtccggc acagtccatc acagtgataa aggttctcag
    13021 tatgtatcgc tggcctacac acagcggctt aaggaagccg gattactggc atcaacagga
    13081 agtactggtg actcgtatga caacgcgatg gcggagagca tcaatggcct ttacaaagcg
    13141 gaggtaatac accgtaagag ctggaaaaac cggacagaag tggagctggc cacactcacg
    13201 tgggtggact ggtataacaa tcgacgattg ctggaaaggc tgggccatat cccaccggca
    13261 gaagcagaaa aagcttatta tgcttccatc ggaaacgatg atctggcagc ctgagttcac
    13321 agataaaaca ctctccagga aacccggggc ggttcatacg ccggagactc tgcgtgtctg
    13381 ggttcgccag catgagcggg ataccggggg cggtgatgga gggctcacca ccgctgaacg
    13441 tcagcgtctg aaagagctgg aacgtgaaaa tcgtgaactg cgccgcagta acgatatcct
    13501 tcgccaggct tccgcttatt ttgcgaaggc ggagttcgac cgcctctgga aaaagtgatg
    13561 ccactgctgg ataagctgcg taagctgtac ggggtcggac cggtatgcag tgaactacat
    13621 attgccccgt caacgtatta ccactgtcag caacagcgac atcatccgga taaacgcagt
    13681 gcccgtgcgc agcgcgatga ctggctgaag aaagagatac agcgcgtata cgatgaaaat
    13741 cacaaggtat acggtgtgcg taaagtctgg cgtcagttgt tacgggaagg tatcagagtg
    13801 gccagatgca ctgtggcacg tctcatggcg gttatgggac ttgccggtgt tctccggggt
    13861 aaaaaggtcc gtacgaccat cagccggaaa gccgttgccg caggcgaccg cgtaaaccgt
    13921 cagttcgtgg cagaacgacc tgaccagctg tgggtggctg attttactta cgtcagcaca
    13981 tggcggggct tcgtctatgt ggcgttcatc attgatgtgt ttgccggata catcgtgggg
    14041 tggcgggtct catcgtccat ggaaacgaca ttcgtgctgg atgcactgga gcaggcgtta
    14101 tgggcccgtc gaccgtccgg cacggtccat cacagtgata aaggttctca gtatgtatcg
    14161 ctggcctaca cacagcggct taaggaagcc ggattactgg catcaacagg aagtacaggc
    14221 gactcgtatg acaacgcgat ggcggagagc atcaatggtc tttacaaagc ggaggtaata
    14281 caccgtaaga gctggaaaaa ccgtgcagaa gtggaactgg ccacactcac gtgggtggac
    14341 tggtataaca atcgacgatt gctggaaagg ctgggccata ctcctccggc agaagcagaa
    14401 aaagcttatt atgcttccat cggaaacgat gatctggcag cctgagttca cagataaaac
    14461 actctccagg aaacccgggg cggttcatcc cttgtcttta tcaaaccacg gaaagtcatc
    14521 cggtaaacgc gccattgtgt gctgccacag gctatttaga gtatgtacac catcctcttt
    14581 agctaaacct cgattaccaa gacacgacct gcacgtcttt ttagagtaat gtgcaaaacc
    14641 gagcgccata caaacctgct tcatcgtcaa tggctcatca tcggctacac tgggacagtt
    14701 agcatggcgg taaaacttat tcgggtgttt ctcgatccac ttggccagtt gtcgtgcatt
    14761 ttcagagagc actctagctc tagctacggc tgtcttagct ataccgacca tcacggtggg
    14821 tatccatttg atatcaccct catagccttt ccccgcaaaa aaacgccaac catatcgttc
    14881 tatcccttca cgatccgttt caaaaacctc gcaatccaca ggcagtgcta acacctcagt
    14941 aatacgtgaa ggtgccgaca tcaacatggc aaaaaccgat gttgtaaata tgtctcgctc
    15001 atgaattggg tcgttggcaa aaatttccgc caacgcatta agagcaatat cactcggaag
    15061 tttatcttcc ctatttttct tcgcctttct gctcgttttg ttcttatcct ctgcacgttt
    15121 aattggattg acccaatttt gtacagcact tgatacaagc tggttttcag ctacaaactt
    15181 agccagccgc tcaatctcac ggccacaatg atatgcggag ccatcggagt agtaatgctt
    15241 tgccaattca gctgcttcat ccagcacaga aatcgacagg ccatcaatat ttgcgtttcc
    15301 atttacttga agcaaagccg cctcaaccgc acggagggct ttactttcat tctttgccct
    15361 agagaggcta tgcccctgct ggtaacggaa ataggctttg gcaaaatcaa tgaaagcagg
    15421 gtcttgcgtc tcttccagta tgggttttct cgtaataatg cccaacttgg caaatacagt
    15481 tatgtttggc caaacaggat cttcccaagt gagatcagag ccgaatacag tcaattgatc
    15541 tcggcactgg gatatgaatg cttcaaggtt ttctgaaccg ccccgggttt cctggagagt
    15601 gttttatctg tgaactcagg ctgccagatc atcgtttccg atggaagcat aataagcttt
    15661 ttctgcttct gccggaggag tatggcccag cctttccagc aatcgtcgat tgttatacca
    15721 gtccacccac gtgagtgtgg ccagttccac ttctgcacgg tttttccagc tcttacggtg
    15781 tattacctcc gctttgtaaa gaccattgat gctctccgcc atcgcgttgt catacgagtc
    15841 gcctgtactt cctgttgatg ccagtaatcc ggcttcctta agccgctgtg tgtaggccag
    15901 cgatacatac tgagaacctt tatcactgtg atggaccgtg ccggacggtc gacgggccca
    15961 taacgcctgc tccagtgcat ccagcacgaa tgtcgtttcc atggacgatg agacccgcca
    16021 ccccacgatg tatccggcaa acacatcaat gatgaacgcc acatagacga agccccgcca
    16081 tgtgctgacg taagtaaaat cagccaccca cagctggtca ggtcgttctg ccacgaactg
    16141 acggtttacg cggtcgcctg cggcaacggc tttccggctg atggtcgtac ggaccttttt
    16201 accccggaga acaccggcaa gtcccataac cgccatgaga cgtgccacag tgcatctggc
    16261 cactctgata ccttcccgta acaactgacg ccagacttta cgcacaccgt ataccttgtg
    16321 attttcatcg tatacgcgct gtatctcttt cttcagccag tcatcgcgct gcgcacgggc
    16381 actgcgttta tccggatgat gtcgctgttg ctgacagtgg taatacgttg acggggcaat
    16441 atgtagttca ctgcataccg gtccgacccc gtacagctta cgcagcttat ccagcagtgg
    16501 catcactttt tccagaggcg gtcgaactcc gccttcgcaa aataagcgga agcctggcga
    16561 aggatatcgt tactgcggcg cagttcacga ttttcacgtt ccagctcttt cagacgctga
    16621 cgttcagcgg tggtgagccc tccatcaccg cccccggtat cccgctcatg ctggcgaacc
    16681 cagacacgca gagtctccgg cgtacagcca atctttggag caatggaaca aattgtcgcc
    16741 cattgtgagt catattcgct ctgactttcc agaaccatac ggactgcccg ttgacggact
    16801 tcggggggaa aaacgagtat ttttagtcat cctgtttacc tctttctcag gaagtttagt
    16861 ctccaggatt cccggggcgg ttcattctgc tgctgtcagc gtagcctttg gcttaaacag
    16921 gaaaatctca gccattggtt attgctccct gttaactaag ctcttcacga cgttttgcac
    16981 aacgcatgat cacatctgcc agcgcgataa tactgcgatc aagtacggca gcaatctgga
    17041 tgtcgccagt tatctctttc acccgttcac gttcgttgag cagtccttga tagacatctt
    17101 catgtgggcc gtccagccaa ggctggaaat gcatgcaggt gtagcaagga ataggaacat
    17161 tggcaccaca aaagccatgt tctccacaag taccaacacc ctgacccatt tcggtccgaa
    17221 tacgactggt agggtcgtta cctcgatgcg cgtctctctc tgaatcgaca agtgccccga
    17281 caaaggcctg agcgtatggt gctaattgga agccaaccgc ctcatcaagc tttttcacat
    17341 gctcggggat attcttgatg taaacgcctg cgttttgggt gtccgcgtgg tcaagcagtt
    17401 ccgcaatcac cagctcgcca aagccttccc gggcagctcg tgtgcctgtg gtataacgaa
    17461 aacgacgagc attgatatgt aatagctcac cggtacgttc cgaccggatg tcggactttt
    17521 ctacgatgta ttggagggtg tcggttatct ctgcggcggg aatgtggagt ttgtcggtag
    17581 catgcagttg gcagaattca taaggagatt ggacggcatc taacacatca agatcaggaa
    17641 aaagagggat ttgctgccgg tcatcttcct gcaattcaaa ccccaaacga ttttcaacct
    17701 gcgcaatcgc atttcttgcc tgagcactca ggattgccca tagttccagt gatattgcaa
    17761 atggtttgaa gtttattcgg aaagagctgc tgcgctgctt ggctcgcggc acattcaaca
    17821 catagaaggg ctctcccttt ccatttttac cacaaaggac atcgaccaca cgaagcagcg
    17881 agatctgaat gggacgacgt cctgtatgac tggtgccaag tgctatagcc aactcagcta
    17941 tagtaattaa gctcttttca taagcccgaa cagccccctc gttaaacgct gtcagctcat
    18001 tatccgtcag tggcccctct tgaggatcca tgcgcttaac cgcatccccc ttacgattac
    18061 cctttagcgt ccagccatct agcaagcggt tcacctcctt actgatgccg ggatagccca
    18121 gttcgtgcca ggatcgaaga aagccacgga tggttgctag ataccattct gttgcaggcg
    18181 acagcatcga ccgataatta atcaataccg tatcagtaat ttctctcgcg tcagtctcac
    18241 gcagcatgtg ctgaaaccgt tcaagaatat tttttgtatg agaagaagaa cgattggatg
    18301 cataaaacgc cagtgtacgc aggcagccat ctagcgtccc atctgacaag taatggtgaa
    18361 tatcagcaag attaatagtg atatttctat ccaatgtcca cgatgtgtca tacaacagaa
    18421 aagagtagcc attggccctg gcacggattc gttctggtac taacccaagt aagaggttat
    18481 tcatggtcat ccttgaagtc ctctggcaag ctagtgccgt tacctgcttg taatgccaag
    18541 gcggcctcaa aacctttttg ccgaatgaaa cgcttgttgt aagtcgctgc ggttcccgag
    18601 ccatctctcc agcccattaa gtacgaacga agttgctctt ggcgttcctc gctgacttgc
    18661 tcattcatgg catccattct ttctgaaaac ttgcgattcc aagtgtggcg gagcgaatgt
    18721 cctgtcgctg catacaattg cggtgatata gacctgacta cggaaaaaat tttatgatat
    18781 ccccctttgg agataggatt tcccagagtt ggtccactct tgtaagttac aaacagatag
    18841 tcatttttct tcgcatttct gactttccgg cgatcttgaa ggatataact atgcagttct
    18901 tgagctaagg attcagccaa cggtaagagc cgttctctag ttttagcatt aggctcattg
    18961 gttctagagt cagcttgttc atctgctctc ctaacgatac ggatcctgtt agcgctaaaa
    19021 tctatgtcct gtattcttat atttaataat tcaccacctc ggatgccaag ataaaacaga
    19081 agtaagatca tcagccgatt gcggcgctgc acatctattg agaaagggtt taactctgat
    19141 ctaggctgga tgacttcaaa tagcgcgtcc aattgaacat cactcaatga cctgtcttgt
    19201 aagtcgtggt ttctgcgctt tttagatggt cggcgcgtct ttatctgcgc cgccatagca
    19261 ttgatctttt cgacaacttc cagttctgtc gtctttaaga tatgcatcgc ataccagtgt
    19321 aaatagttgg caaacgttgt tagccgggaa tgctgtgtcc cgttatcaac gatatcggtc
    19381 atcgcatcgg ccaaccaaga ggtagaggac acagttaatg gcccctttct ttgtttgcac
    19441 tgagcaaaat ctcgcagatc atccagctca tgtggcttga agaagtcttt ggtcaaaaac
    19501 cgctgctcaa ggtcaatgtc gcgactctct acaaagcgta acaacataac caggttgtta
    19561 gccgccgcca acatcgttga aaaagcatcc tctctattgc ggatttgagt tgttagaaac
    19621 agagtggggt aatacatggg aagcccagaa gcacgatcca ctaccatgca atagcgctcg
    19681 ccactatcca tgacaaatgt tgtaactctg tagttctttt ccatgcactc acaccccata
    19741 aacattacac aattacaagg tacactttat gtgaaaaatc accaatttat acaagacact
    19801 tttttccgac gatagaaaca aaaaacccct gaattaacag gggcctgcgt atggattctt
    19861 tacaaataac tcacaagtta actctagaac ggaatgtcgt catcgaagtc atacgcgggt
    19921 tcagacggct gctgaggctc cggctgcgcg gccttacgtc cacggccttt cgttttcgcg
    19981 cctccttttt tcggttccgg ctgtgattcc ggctgaggct gaccgctgaa ctgttgcgcc
    20041 tcttccggct gagtctgagt acctgcggca cgtcccagca tctgcatggt gcccgtggtc
    20101 ttaacaagaa tttcggtgac gtaacgggtg ataccgttat cctcccagct acgggtgcga
    20161 agctgacctt cgatgtagac ctgcgcgccc ttgcgcagat actcaccagc cacttccgcg
    20221 agcttgccga acagcaccac gcgatgccat tctgtctgct cccgtatctc ccccgtctgt
    20281 ttgtcgcgcc agctttctga cgtggccacc tgcaggtttg ccactgcgcc cccgtttggg
    20341 atgtaacgga cttccggatc ttttcccaga cgaccgacaa gaatgacctt gttaatgcca
    20401 cgaactgcca tgatgtgtct ccttctgttg at
//
