LOCUS       NZ_CP083506              739 bp    DNA     linear   CON 08-JAN-2024
DEFINITION  Escherichia coli strain elppa5 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP083506
VERSION     NZ_CP083506.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21169128
            Assembly: GCF_020827635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Huang,Y.T.
  TITLE     Genome sequencing and analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 739)
  AUTHORS   Huang,Y.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) Department of Computer Science and
            Information Engineering, National Chung Cheng University, No.168,
            Sec. 1, University Rd., Min-Hsiung Town, Chiayi City 621, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 59x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:44:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,279
            CDSs (total)                      :: 5,157
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 79 of 219
            Pseudo Genes (incomplete)         :: 152 of 219
            Pseudo Genes (internal stop)      :: 42 of 219
            Pseudo Genes (multiple problems)  :: 50 of 219
            Pseudo Genes (short protein)      :: 4 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083506.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="elppa5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="Taiwan"
                     /lat_lon="24 N 120 E"
                     /collection_date="2019-11"
                     /collected_by="National Chung Cheng University"
     gene            <1..196
                     /locus_tag="K9U08_RS25730"
                     /old_locus_tag="K9U08_25740"
     CDS             <1..196
                     /locus_tag="K9U08_RS25730"
                     /old_locus_tag="K9U08_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_228588617.1"
                     /translation="SIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAID
                     AELERLRHAVLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            186..>739
                     /locus_tag="K9U08_RS25735"
                     /old_locus_tag="K9U08_25745"
     CDS             186..>739
                     /locus_tag="K9U08_RS25735"
                     /old_locus_tag="K9U08_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_159183593.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQRDGAIVLQGKPEEVRELID
                     ASPYAKKYTSGVLSFAEQDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLE
                     LNFLVPNTELLTGRRLQPYYDRADRPRIDAWQTVVNGRLGLHDPNAPENRRALVTPSSL
                     PKTKQEAAEAITRGLLALASSG"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          187..596
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="81.752"
                     /coverage="83.0"
                     /evalue="9.86e-74"
ORIGIN
        1 gtccatcgat ccggttttgc tgcgccagct tgccgggatg gggaacaacc tcaaccagat
       61 cgcccggaaa attaacggcg gccagtggtc aggcgctgac cgggttcagg tggtggccgc
      121 gctgatggcc attgatgccg aactcgagcg gctgcggcat gccgtgctgg aaaagggggc
      181 agacgatgat cgttaagttt catccgcgag ggcgaggcgg cggtgccggg ccggtggatt
      241 atctgctggg gaaagaccgc cagcgcgacg gggccatcgt cctgcaggga aagccggagg
      301 aggtccggga gcttatcgac gcctcgccct acgccaaaaa gtacacctcc ggcgtcctgt
      361 cttttgctga acaggattta ccgcccggcc agcgtgaaaa gctgatggcg agcttcgagc
      421 gggttctgat gcccggactc gataaagacc agtacagcgt gctgtgggtt gaacaccggg
      481 acaaggggcg gctggaactg aattttctgg taccgaacac ggaactgctg accggcaggc
      541 gtctccagcc gtactacgac cgggcagacc gtccgcgcat cgatgcgtgg cagaccgtgg
      601 tgaacgggcg actggggctg cacgacccga acgcaccgga gaaccggcgc gcgctggtga
      661 cgccatcttc gttacccaaa acgaagcagg aagccgcaga ggctattacg cggggcttac
      721 tggccctcgc ctcgtcagg
//
