LOCUS       NZ_CP083505             1301 bp    DNA     linear   CON 08-JAN-2024
DEFINITION  Escherichia coli strain elppa5 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP083505
VERSION     NZ_CP083505.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21169128
            Assembly: GCF_020827635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Huang,Y.T.
  TITLE     Genome sequencing and analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Huang,Y.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) Department of Computer Science and
            Information Engineering, National Chung Cheng University, No.168,
            Sec. 1, University Rd., Min-Hsiung Town, Chiayi City 621, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 59x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:44:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,279
            CDSs (total)                      :: 5,157
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 79 of 219
            Pseudo Genes (incomplete)         :: 152 of 219
            Pseudo Genes (internal stop)      :: 42 of 219
            Pseudo Genes (multiple problems)  :: 50 of 219
            Pseudo Genes (short protein)      :: 4 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083505.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="elppa5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="Taiwan"
                     /lat_lon="24 N 120 E"
                     /collection_date="2019-11"
                     /collected_by="National Chung Cheng University"
     gene            complement(190..348)
                     /locus_tag="K9U08_RS26465"
     CDS             complement(190..348)
                     /locus_tag="K9U08_RS26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     Typing          823..902
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(240..637)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
ORIGIN
        1 aactgacgat catttaaagg atcttcttga gatccttttt ttctgcgcgt aatctcttgc
       61 actgtaaacg aaaaaaccgc ctggggaggc ggtttgatcg aaggttaagt cagttgggga
      121 actgcttaac cgggtaactg ggtatacgag accgcagata ccaaaactgt tctttcagtg
      181 tagccgcagt taggcaccca cttcaagacc tctcaatatc tctctcgtat atatccagtt
      241 accaatggct gctgccagtg gcgtttttgc gtgtccgtcc gggttggact caagatgata
      301 gttaccggaa agggcgcagc agtcgggctg aacggggggt tcgtgcatac agcccagctt
      361 ggagcgaacg acctaaaccg agccgagata ccagcagcgt gagctatgag aaagcgccac
      421 gcttcccgaa agggaaaaag gcggaacagg tatccggtaa acggcagggt cggaacagga
      481 gagcgcacga gggagccgcc ggagagaaat caccggcatc tttaagtcct gtcgggtttc
      541 gtcctcctct gacttgagcg tccatttctg tgatgctcgt caggggaggc ggagcctatg
      601 gaaaaacggc aacgccgcat ccttttctcc ggccctttct gatttgagcg cctttgcttt
      661 atcccgttat ccgtgagtcg gtcacgcccg ccgcagccga acggcaggag cgcagctacc
      721 gagtgagcga ggaggcgtca tttcatctgt ccgtgcatgg tattgcggac gctcctgagc
      781 cacatatcac acctgccaca tgaggcactt tcatgaatcg tgctctcatg ccaacatagt
      841 aagccagtat acacagcgta aacgctgtga ctggttcagg gctgcgcccc gaaacccgct
      901 aacaccactg acgcgctggc gcttgtccaa cgccggagcg acccggaaaa cttttccgtg
      961 ccgtccggcg tttgcgaagg agggataatg cagaagccga agaagaagcc gctcgtcgta
     1021 aaaaaagatc ctggcatccg gcagtgatgg caaatctgat gcgtcaggca caggaggaag
     1081 attaccgccg ggagcagatg gcagagcttg agcgttcagc ccggtcacat tttgcggata
     1141 tcggaggaga ggatggcggt taagcgtacc cgttttttag gtatccgggt gaccgatggg
     1201 gagtaccagc agctgctgga gcgctgcaat ggaaggcaac tggcggtgtg gatgcgggaa
     1261 acctgtctcg atacgcgccc ggcgcggtcg ttgcggctgc c
//
