LOCUS       NZ_CP083500            10760 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Escherichia coli strain elppa6 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP083500
VERSION     NZ_CP083500.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21169129
            Assembly: GCF_020827615.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Huang,Y.T.
  TITLE     Genome sequencing and analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10760)
  AUTHORS   Huang,Y.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) Department of Computer Science and
            Information Engineering, National Chung Cheng University, No.168,
            Sec. 1, University Rd., Min-Hsiung Town, Chiayi City 621, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 108x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:13:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,016
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,682
            CDSs (with protein)               :: 4,682
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 214
            CDSs (without protein)            :: 214
            Pseudo Genes (ambiguous residues) :: 0 of 214
            Pseudo Genes (frameshifted)       :: 91 of 214
            Pseudo Genes (incomplete)         :: 129 of 214
            Pseudo Genes (internal stop)      :: 45 of 214
            Pseudo Genes (multiple problems)  :: 49 of 214
            Pseudo Genes (short protein)      :: 2 of 214
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083500.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="elppa6"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="Taiwan"
                     /lat_lon="24 N 120 E"
                     /collection_date="2019-01"
                     /collected_by="National Chung Cheng University"
     gene            join(<10743..10760,1..1224)
                     /locus_tag="K9U09_RS24165"
                     /old_locus_tag="K9U09_24185"
                     /pseudo=""
     CDS             join(<10743..10760,1..1224)
                     /locus_tag="K9U09_RS24165"
                     /old_locus_tag="K9U09_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1227..1487
                     /locus_tag="K9U09_RS24170"
                     /old_locus_tag="K9U09_24190"
     CDS             1227..1487
                     /locus_tag="K9U09_RS24170"
                     /old_locus_tag="K9U09_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000420692.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            1549..1692
                     /locus_tag="K9U09_RS24175"
                     /old_locus_tag="K9U09_24195"
     CDS             1549..1692
                     /locus_tag="K9U09_RS24175"
                     /old_locus_tag="K9U09_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            2187..3122
                     /locus_tag="K9U09_RS24180"
                     /old_locus_tag="K9U09_24200"
     CDS             2187..3122
                     /locus_tag="K9U09_RS24180"
                     /old_locus_tag="K9U09_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271369.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSTSA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(3316..3714)
                     /locus_tag="K9U09_RS24185"
                     /old_locus_tag="K9U09_24205"
     CDS             complement(3316..3714)
                     /locus_tag="K9U09_RS24185"
                     /old_locus_tag="K9U09_24205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222916.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            3891..4817
                     /locus_tag="K9U09_RS24190"
                     /old_locus_tag="K9U09_24210"
     CDS             3891..4817
                     /locus_tag="K9U09_RS24190"
                     /old_locus_tag="K9U09_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001314449.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            4820..5353
                     /locus_tag="K9U09_RS24195"
                     /old_locus_tag="K9U09_24215"
     CDS             4820..5353
                     /locus_tag="K9U09_RS24195"
                     /old_locus_tag="K9U09_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094316166.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVAREILRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            5575..7304
                     /locus_tag="K9U09_RS24200"
                     /old_locus_tag="K9U09_24220"
                     /pseudo=""
     CDS             5575..7304
                     /locus_tag="K9U09_RS24200"
                     /old_locus_tag="K9U09_24220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7307..7567
                     /locus_tag="K9U09_RS24205"
                     /old_locus_tag="K9U09_24225"
     CDS             7307..7567
                     /locus_tag="K9U09_RS24205"
                     /old_locus_tag="K9U09_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000420692.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            7629..7772
                     /locus_tag="K9U09_RS24210"
                     /old_locus_tag="K9U09_24230"
     CDS             7629..7772
                     /locus_tag="K9U09_RS24210"
                     /old_locus_tag="K9U09_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            8267..9202
                     /locus_tag="K9U09_RS24215"
                     /old_locus_tag="K9U09_24235"
     CDS             8267..9202
                     /locus_tag="K9U09_RS24215"
                     /old_locus_tag="K9U09_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271369.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSTSA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(9396..9794)
                     /locus_tag="K9U09_RS24220"
                     /old_locus_tag="K9U09_24240"
     CDS             complement(9396..9794)
                     /locus_tag="K9U09_RS24220"
                     /old_locus_tag="K9U09_24240"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222916.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            9971..>10759
                     /locus_tag="K9U09_RS24225"
                     /old_locus_tag="K9U09_24245"
                     /pseudo=""
     CDS             9971..>10759
                     /locus_tag="K9U09_RS24225"
                     /old_locus_tag="K9U09_24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     BGC             1..10760
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2188..3122
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.551"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8268..9202
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.551"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3892..4814
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9972..10759
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.099"
                     /coverage="100.0"
                     /evalue="5.5300000000000005e-173"
ORIGIN
        1 gaccaggcaa ctgttagcgt gacaaaacgt gtgaccgatg ttgtgaagga cacacgacag
       61 catattgccg tagttgcagg tgtacctatg agtgtgcctg ttgtgaatgc taaaccaaca
      121 cgtaccccag gtgtatttca cgcatcattt cctggtgtcc cttctctgac agtcagcacg
      181 gtcaaaggtc ttcctgtgtc aacaaacctt ccccgtggta ttacggagga taagggccgg
      241 actgccgttc ctgcaggatt tacctttggt ggtggttcac atgaagcggt gatccgtttc
      301 ccgaaagaaa gtgggcagaa gccgatttat gtatcagtga cagatgttct tacccctgca
      361 caggtaaaac aacgtcagga tgaggaaaat cgcctccagc aggaatggaa tgacacacat
      421 cctgtagaag tggctgaacg caattatgaa caggcccgcg cagaactgga tcaggctaat
      481 aaagatgttg ccagaaacca ggaacgacag gctaaagctg ttcaggttta taattctcgt
      541 aaaagtgaac ttgatgcagc gaataaaact cttgctgatg caaaggctga aataaaacaa
      601 ttcgagcgat ttgcccgaga accaatggct gctggtcacc gaatgtggca aatggcaggg
      661 cttaaggccc agcgggcaca gacggatgta aataataaga aggctgcatt tgatgctgct
      721 gcaaaagata agtctgatgc tgatgctgcg ttaggtgctg cgctggaacg ccgtaagcag
      781 aaggaaaata aagaaaagga ctctaaggat aaattagata aggagagtaa acggaataag
      841 ccagggaagg cgacgggtaa aggaaaacca gttggtgata aatggcttga tgatgcgggt
      901 aaagattcag gagctccaat tcctgatcgc attgctgata agttgcgtga taaagaattt
      961 aaaagttttg acgatttccg gaagaaattc tgggaagaag tgtcaaaaga tcccgagctt
     1021 agtaagcaat ttaaagacag taataagacg aacattcaaa agggaaaagc accttttgca
     1081 aggaagaaag accaagtagg tggtagggaa cgctttgaat tacatcatga taaaccaatc
     1141 agtcaggatg gtgatgtcta tgatatggat aatatcagag tgaccacacc taagcgacat
     1201 attgatattc atagaggtaa gtaaaaatgg aactgaaaca tagtattagt gattataccg
     1261 aggctgaatt tctggagttt gtaaaaaaaa tatgtagagc tgaaggtgct actgaagagg
     1321 atgacaataa attagtgaga gagtttgagc gattaactga gcacccagat ggttcagatc
     1381 tgatttatta tcctcgcgat gacagggaag atagtcctga agggattgtt aaagaaatta
     1441 aagaatggcg agctgctaac ggtaagccag gatttaaaca gggctaaaat atgaatgccg
     1501 gttgtttatg gatgaatggc tggcattctt tcacaacaag gagtcgttat gaaaaaaata
     1561 acagggatta ttttattgct tcttgcagtc attattctgg ctgcatgtca ggcaaactat
     1621 atccgggatg ttcagggcgg gactgtatcc ccgtcatcaa cagctgaagt gaccggatta
     1681 gcaacgcagt aacccgaaat cctctttgac aaaaacaaag cgtgtcaggc tgattctgat
     1741 gcgccttttt ttgaaatgtc acaaaaattc catgcgggag atgagagcta aaatccccgt
     1801 gcagaacttt ccacccaggg ggagaaaact tgtcattttg acctgttcgc ccttcggaac
     1861 agtcgaaatc gatcgcgcat cgctttcgtg catagttatg cagccctcta aaaacgattc
     1921 tggcgcgttt ttctggttta gcctggtgtt tccttgtctt tttgcgtttt ttgcgccaga
     1981 aagcgattga gggcgtttta cagggtacgt acaacgggag ttatggtaaa tggatcgggt
     2041 tttcgggaag gatcgacagg atttgccgtt gggtgtagtg taagcgactg aaaaacaaac
     2101 gcccctgaaa ttcgtgtctc tccgccaaga ttgtcacgag aatacagggg cgaggtcggt
     2161 cgataaccta gaaggaatcg taacacatga gcgccgcgct tcaatacttc gaagaaaatt
     2221 tacccctccg cccctatcac acggatgatc tcgctttcgg ccttcgtatc tccggtaaag
     2281 ggcgcgcgct tcttgcacgg tatatccagc agaaccaacc tcatgcgcag ttctggctgg
     2341 tttttgacgt tgaccgcgag ggggctgcga ttgactggag cgacctgaac gcccccgcac
     2401 cgaacatcac cataaaaaat cccgtgaacg gtcacgccca cctgctctac gcactcaata
     2461 tcgccgtgag aaccgcgcct gatgcttcgg ttaaggcgct gaaatacgcc gccgcgattg
     2521 aacgtgcgtt gtgtgaaaaa ctgggcgcag atgtaaacta cagcggactg atttgcaaaa
     2581 atccgttcca tctggaatgg caggtgatgg agtggcgtga ggaagcctat accctcgatg
     2641 aactggctga ttatctcgat ttgagcactt cagcgcgccg tagcatcgat aaacattacg
     2701 ggatggggcg aaactgccat ctgttcgaaa tgacgcgcaa atgggcttac agggcgattc
     2761 gtcagggctg gccagcattc tcacagtggc ttgatgccgt gattcagcgt gtcgaaatgt
     2821 acaacgcatc gcttcccgtt ccgctttcac ctcctgaatg tcgggctatt ggcaagagta
     2881 ttgcgaaata cacgcacagg aacttcacgg cggaaacttt cgcacagtat gtggctgata
     2941 cgcacacgcc agaaatacag gccaagagag gcaggaaagg tggcatcgct aaaggcgaag
     3001 cctacgatga caagcgtttc atggcgctat gtatgctgga gaatggatat tctcagaaag
     3061 ctattgcggc gatgttggag gtttctactc gaaccattcg aaactggaaa agcggaaaat
     3121 agcctatatc agataacagc gcctttctgg cgtttttttg agcagtaggt cttttgccgt
     3181 ttttccccgt tcagccctgc aatggtgctg ttttccgcat gattgtgctg gtagtgctcg
     3241 ccgcagtctc atgaccgagc gtagcgagcg aatgagcgag gaagcaccag ggaaccggaa
     3301 cacggatgga ggcacttacg cgtctgggtt tggggatgtc tccagatcgt acttaatttc
     3361 cttaagtgcc tgtaatatcc tgtctttttt atagcatagc tcgcctgttt ttgtgtctac
     3421 cagtcctgct tcgatcagta gcccagccgc gagaaagagt cggtgattgc gttctcgtcg
     3481 ttgttttttt ccctcgattc gtttttggac cgcctgtttt cgttcgctct cctgtgcctg
     3541 taagtcgttt ttttccgctg ttgccagtac gtggaggagt ttgcgttcct gttctgtgag
     3601 tcctgcttcg ttgtcgagca ggtcaataat ctgtctctgt cgtgcggtct gttctccgag
     3661 cttcctgatg aatccgatcc gtttcttcag ccattcgatg ttgctgatgc gcatgttcgc
     3721 ggtgctccct ggtgtttttt tgcggtgtgt tgtggtcgtg cagtatatcg cttgcggtcg
     3781 gtgggtgcag ggggtatctt ccgtgctaat tcgtaaaatt agaaggaagc gcacttatat
     3841 gtttctctcc ttcgtcgaga aacagtgcgc tcctgcggag ggctgaaacc gtggcaacct
     3901 atcacctcag cgttaaattt ggtggcaagg ggcaggctgc aaaccacgcc gattacatcg
     3961 agcgcaaaga gaaataccgc gaccgtcagg atttggaata ttccgcacac ggaaacatgc
     4021 cggaatgggc gcgagataat ccctcacatt tctggcaggc ggctgaccag tttgaacgtg
     4081 cgaacggttc gacctatcgg gaacttgaaa ttgcattacc ccgtgaactg acagcggaac
     4141 agcgccttga actggtgcag gattttgtac gtcaggaagc tggcgaacgg cacgcctgga
     4201 gttttgccat tcacaatccg aaagcgagca ttgacggcgg cgaacagcct catgcgcata
     4261 tcatgatgag ccagcgtgtg aatgatggca ttgaacgtac accggaacag tatttccggc
     4321 gctataacgc caggtatccc gagcggggag gtgcgaaaaa ggattccgga agcctgactc
     4381 tcactcagca aaaagagcag ttgcgggaat tgcgaaaacg ctgggaggtg aagcacaacg
     4441 aacatatgag aaagcatggc tttgaaaggg gcttcattga ctgccggacg ctgaaagaac
     4501 agggcattga acgacgtccc gaagtgcatc tgggttttga agctgccgga cggcttgatg
     4561 atgcccagcg tcatgacatc atgcaaaaac ggtctgaacc ggactattcc cgccatcttg
     4621 aacaggcgcg aacggtcggg catgacatca gggaatggct ggacactccg gttaagcaac
     4681 atgaactggt cagaaggctg caacatgatg ttgcgcggga gcatggcttt gacatgagag
     4741 acagagtgag cagacagaac cgaactgaac ctgaacgggt taaatcaccg tcacgtggat
     4801 tttctatggg attctgaaca tggccagcac agaagaaaaa ttactcacgc tcctgttatc
     4861 ggtggagcat ctgcaacata ccgcaatgga gcaacagaaa gctgttgctg attctctggc
     4921 tgtggcaagg gaaatacttc gcggatatcg ggatatttct gacggtgtgg caaaacaggt
     4981 cagggatgaa gtcagaaacg aggtgcagaa gctggatgtt gcagggctga taggtgagcg
     5041 catcagccag gattttcagc ggctggaaga gtcagtcgca aacatgaagg caaacgcgga
     5101 tgccctgaac agggatattg cgacaattca cagcaatatc gtgcaggaat acaacaccct
     5161 taaagggtat agctggaaat ggctgggtgg ggtgtcagtg gcttttctgg ttatgttctg
     5221 tgtgcttgga tggggaatga aaacaatgct cgacaggaac tacaacgcca cagcggcggt
     5281 gtatcagaaa gttgtgacac tggaagataa actggcggag aaacctgtca cgggcaagag
     5341 ccggaaaaac tgacgcgctt cgcttgtcca acgcctcacg acccgcgaaa ggatttcgcg
     5401 ttctgttcac cgttttcatg agtgattcct ccgaactgcc aaaaaatatc ctccccctga
     5461 cttgacagaa aaaataatgg cgagtacttt ttgatctgta cataaaacca gtggttttat
     5521 gtacagtatt aactatgtaa ttaattgttt taacgcttaa aagagggaat ttttatgagc
     5581 ggtggagatg gacgcgacca caacagtggc gcacataaca caggtggtaa cattaatggc
     5641 ggccctacgg ggcttggtgg aaatggtggg gcttctgacg gctccggatg gagttcggaa
     5701 aataacccat ggggtggcgg ttctggtagt ggtgttcact ggggaggtgg ctccggccat
     5761 ggcaatggcg gggggaatgg taattccggt ggtggcagca attcatccgt agcagcaccg
     5821 tggcatttgg ttttcctgct ttggcagctc ctggtgccgg aacactgggt atcgccgtct
     5881 ctggtgaggc tttatctgcg gcaatagcgg atatctttgc agccctgaaa ggtccgttta
     5941 aattcagtgc atggggtatt gcgctttacg gcattctgcc atctgaaata gcaaaagatg
     6001 acccgaaaat gatgtcaaag attgtgacgt cattaccggc agaaacagta acaaatgttc
     6061 aggtcagtac acttccgctg gaccaggcaa ctgttagcgt gacaaaacgt gtgaccgatg
     6121 ttgtgaagga cacacgacag catattgccg tagttgcagg tgtacctatg agtgtgcctg
     6181 ttgtgaatgc taaaccaaca cgtaccccag gtgtatttca cgcatcattt cctggtgtcc
     6241 cttctctgac agtcagcacg gtcaaaggtc ttcctgtgtc aacaaacctt ccccgtggta
     6301 ttacggagga taagggccgg actgccgttc ctgcaggatt tacctttggt ggtggttcac
     6361 atgaagcggt gatccgtttc ccgaaagaaa gtgggcagaa gccgatttat gtatcagtga
     6421 cagatgttct tacccctgca caggtaaaac aacgtcagga tgaggaaaat cgcctccagc
     6481 aggaatggaa tgacacacat cctgtagaag tggctgaacg caattatgaa caggcccgcg
     6541 cagaactgga tcaggctaat aaagatgttg ccagaaacca ggaacgacag gctaaagctg
     6601 ttcaggttta taattctcgt aaaagtgaac ttgatgcagc gaataaaact cttgctgatg
     6661 caaaggctga aataaaacaa ttcgagcgat ttgcccgaga accaatggct gctggtcacc
     6721 gaatgtggca aatggcaggg cttaaggccc agcgggcaca gacggatgta aataataaga
     6781 aggctgcatt tgatgctgct gcaaaagata agtctgatgc tgatgctgcg ttaggtgctg
     6841 cgctggaacg ccgtaagcag aaggaaaata aagaaaagga ctctaaggat aaattagata
     6901 aggagagtaa acggaataag ccagggaagg cgacgggtaa aggaaaacca gttggtgata
     6961 aatggcttga tgatgcgggt aaagattcag gagctccaat tcctgatcgc attgctgata
     7021 agttgcgtga taaagaattt aaaagttttg acgatttccg gaagaaattc tgggaagaag
     7081 tgtcaaaaga tcccgagctt agtaagcaat ttaaagacag taataagacg aacattcaaa
     7141 agggaaaagc accttttgca aggaagaaag accaagtagg tggtagggaa cgctttgaat
     7201 tacatcatga taaaccaatc agtcaggatg gtgatgtcta tgatatggat aatatcagag
     7261 tgaccacacc taagcgacat attgatattc atagaggtaa gtaaaaatgg aactgaaaca
     7321 tagtattagt gattataccg aggctgaatt tctggagttt gtaaaaaaaa tatgtagagc
     7381 tgaaggtgct actgaagagg atgacaataa attagtgaga gagtttgagc gattaactga
     7441 gcacccagat ggttcagatc tgatttatta tcctcgcgat gacagggaag atagtcctga
     7501 agggattgtt aaagaaatta aagaatggcg agctgctaac ggtaagccag gatttaaaca
     7561 gggctaaaat atgaatgccg gttgtttatg gatgaatggc tggcattctt tcacaacaag
     7621 gagtcgttat gaaaaaaata acagggatta ttttattgct tcttgcagtc attattctgg
     7681 ctgcatgtca ggcaaactat atccgggatg ttcagggcgg gactgtatcc ccgtcatcaa
     7741 cagctgaagt gaccggatta gcaacgcagt aacccgaaat cctctttgac aaaaacaaag
     7801 cgtgtcaggc tgattctgat gcgctttttt ttgaaatgtc acaaaaattc catgcgggag
     7861 atgagagcta aaatccccgt gcagaacttt ccacccaggg ggagaaaact tgtcattttg
     7921 acctgttcgc ccttcggaac agtcgaaatc gatcgcgcat cgctttcgtg catagttatg
     7981 cagccctcta aaaacgattc tggcgcgttt ttctggttta gcctggtgtt tccttgtctt
     8041 tttgcgtttt ttgcgccaga aagcgattga gggcgtttta cagggtacgt acaacgggag
     8101 ttatggtaaa tggatcgggt tttcgggaag gatcgacagg atttgccgtt gggtgtagtg
     8161 taagcgactg aaaaacaaac gcccctgaaa ttcgtgtctc tccgccaaga ttgtcacgag
     8221 aatacagggg cgaggtcggt cgataaccta gaaggaatcg taacacatga gcgccgcgct
     8281 tcaatacttc gaagaaaatt tacccctccg cccctatcac acggatgatc tcgctttcgg
     8341 ccttcgtatc tccggtaaag ggcgcgcgct tcttgcacgg tatatccagc agaaccaacc
     8401 tcatgcgcag ttctggctgg tttttgacgt tgaccgcgag ggggctgcga ttgactggag
     8461 cgacctgaac gcccccgcac cgaacatcac cataaaaaat cccgtgaacg gtcacgccca
     8521 cctgctctac gcactcaata tcgccgtgag aaccgcgcct gatgcttcgg ttaaggcgct
     8581 gaaatacgcc gccgcgattg aacgtgcgtt gtgtgaaaaa ctgggcgcag atgtaaacta
     8641 cagcggactg atttgcaaaa atccgttcca tctggaatgg caggtgatgg agtggcgtga
     8701 ggaagcctat accctcgatg aactggctga ttatctcgat ttgagcactt cagcgcgccg
     8761 tagcatcgat aaacattacg ggatggggcg aaactgccat ctgttcgaaa tgacgcgcaa
     8821 atgggcttac agggcgattc gtcagggctg gccagcattc tcacagtggc ttgatgccgt
     8881 gattcagcgt gtcgaaatgt acaacgcatc gcttcccgtt ccgctttcac ctcctgaatg
     8941 tcgggctatt ggcaagagta ttgcgaaata cacgcacagg aacttcacgg cggaaacttt
     9001 cgcacagtat gtggctgata cgcacacgcc agaaatacag gccaagagag gcaggaaagg
     9061 tggcatcgct aaaggcgaag cctacgatga caagcgtttc atggcgctat gtatgctgga
     9121 gaatggatat tctcagaaag ctattgcggc gatgttggag gtttctactc gaaccattcg
     9181 aaactggaaa agcggaaaat agcctatatc agataacagc gcctttctgg cgtttttttg
     9241 agcagtaggt cttttgccgt ttttccccgt tcagccctgc aatggtgctg ttttccgcat
     9301 gattgtgctg gtagtgctcg ccgcagtctc atgaccgagc gtagcgagcg aatgagcgag
     9361 gaagcaccag ggaaccggaa cacggatgga ggcacttacg cgtctgggtt tggggatgtc
     9421 tccagatcgt acttaatttc cttaagtgcc tgtaatatcc tgtctttttt atagcatagc
     9481 tcgcctgttt ttgtgtctac cagtcctgct tcgatcagta gcccagccgc gagaaagagt
     9541 cggtgattgc gttctcgtcg ttgttttttt ccctcgattc gtttttggac cgcctgtttt
     9601 cgttcgctct cctgtgcctg taagtcgttt ttttccgctg ttgccagtac gtggaggagt
     9661 ttgcgttcct gttctgtgag tcctgcttcg ttgtcgagca ggtcaataat ctgtctctgt
     9721 cgtgcggtct gttctccgag cttcctgatg aatccgatcc gtttcttcag ccattcgatg
     9781 ttgctgatgc gcatgttcgc ggtgctccct ggtgtttttt gcggtgtgtt gtggtcgtgc
     9841 agtatatcgc ttgcggtcgg tgggtgcagg ggggtatctt ccgtgctaat tcgtaaaatt
     9901 agaaggaagc gcacttatat gtttctctcc ttcgtcgaga aacagtgcgc tcctgcggag
     9961 ggctgaaacc gtggcaacct atcacctcag cgttaaattt ggtggcaagg ggcaggctgc
    10021 aaaccacgcc gattacatcg agcgcaaaga gaaataccgc gaccgtcagg atttggaata
    10081 ttccgcacac ggaaacatgc cggaatgggc gcgagataat ccctcacatt tctggcaggc
    10141 ggctgaccag tttgaacgtg cgaacggttc gacctatcgg gaacttgaaa ttgcattacc
    10201 ccgtgaactg acagcggaac agcgccttga actggtgcag gattttgtac gtcaggaagc
    10261 tggcgaacgg cacgcctgga gttttgccat tcacaatccg aaagcgagca ttgacggcgg
    10321 cgaacagcct catgcgcata tcatgatgag ccagcgtgtg aatgatggca ttgaacgtac
    10381 accggaacag tatttccggc gctataacgc caggtatccc gagcggggag gtgcgaaaaa
    10441 ggattccgga agcctgactc tcactcagca aaaagagcag ttgcgggaat tgcgaaaacg
    10501 ctgggaggtg aagcacaacg aacatatgag aaagcatggc tttgaaaggg gcttcattga
    10561 ctgccggacg ctgaaagaac agggcattga acgacgtccc gaagtgcatc tgggttttga
    10621 agctgccgga cggcttgatg atgcccagcg tcatgacatc atgcaaaaac ggtctgaacc
    10681 ggactattcc cgccatcttg aacaggcgcg aacggtcggg catgacatca gggaatggct
    10741 ggacactccg gttaagcaac
//
