LOCUS       NZ_CP083266             1865 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Escherichia coli strain JNU plasmid p3, complete sequence.
ACCESSION   NZ_CP083266
VERSION     NZ_CP083266.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20959237
            Assembly: GCF_020034635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Han,H., Li,Z., Cui,W. and Zhou,Z.
  TITLE     Whole genome sequence of an Escherichia coli strain isolated from
            environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Han,H., Li,Z., Cui,W. and Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2021) School of Biotechnology, Jiangnan
            University, No. 1800, Lihu Avenue, Wuxi, Jiangsu 214122, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 12-AUG-2021
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 203.51x
            Sequencing Technology :: Illumina Novaseq6000; Oxford Nanopore
            PromethION 48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:50:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,843
            CDSs (total)                      :: 4,725
            Genes (coding)                    :: 4,577
            CDSs (with protein)               :: 4,577
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 64 of 148
            Pseudo Genes (incomplete)         :: 79 of 148
            Pseudo Genes (internal stop)      :: 47 of 148
            Pseudo Genes (multiple problems)  :: 38 of 148
            Pseudo Genes (short protein)      :: 2 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083266.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JNU"
                     /isolation_source="Environmental waters"
                     /db_xref="taxon:562"
                     /plasmid="p3"
                     /geo_loc_name="China:Jiangsu"
                     /collection_date="2006-09-10"
                     /collected_by="Zhou Li"
     gene            583..1443
                     /locus_tag="K6T58_RS24130"
                     /old_locus_tag="K6T58_24050"
     CDS             583..1443
                     /locus_tag="K6T58_RS24130"
                     /old_locus_tag="K6T58_24050"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-116"
                     /protein_id="WP_000027050.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-116"
     ARG             584..1440
                     /name="blaTEM-116"
                     /gene="blaTEM-116"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-116"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
       61 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      121 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
      181 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
      241 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
      301 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
      361 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
      421 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      481 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      541 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      601 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      661 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      721 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
      781 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
      841 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      901 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      961 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1021 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     1081 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     1141 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     1201 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     1261 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     1321 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     1381 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     1441 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     1501 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     1561 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1621 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1681 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1741 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1801 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1861 ggcga
//
