LOCUS       NZ_CP083265             4543 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Escherichia coli strain JNU plasmid p2, complete sequence.
ACCESSION   NZ_CP083265
VERSION     NZ_CP083265.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20959237
            Assembly: GCF_020034635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Han,H., Li,Z., Cui,W. and Zhou,Z.
  TITLE     Whole genome sequence of an Escherichia coli strain isolated from
            environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Han,H., Li,Z., Cui,W. and Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2021) School of Biotechnology, Jiangnan
            University, No. 1800, Lihu Avenue, Wuxi, Jiangsu 214122, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 12-AUG-2021
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 203.51x
            Sequencing Technology :: Illumina Novaseq6000; Oxford Nanopore
            PromethION 48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:50:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,843
            CDSs (total)                      :: 4,725
            Genes (coding)                    :: 4,577
            CDSs (with protein)               :: 4,577
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 64 of 148
            Pseudo Genes (incomplete)         :: 79 of 148
            Pseudo Genes (internal stop)      :: 47 of 148
            Pseudo Genes (multiple problems)  :: 38 of 148
            Pseudo Genes (short protein)      :: 2 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083265.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JNU"
                     /isolation_source="Environmental waters"
                     /db_xref="taxon:562"
                     /plasmid="p2"
                     /geo_loc_name="China:Jiangsu"
                     /collection_date="2006-09-10"
                     /collected_by="Zhou Li"
     gene            complement(598..789)
                     /locus_tag="K6T58_RS24095"
                     /old_locus_tag="K6T58_24015"
     CDS             complement(598..789)
                     /locus_tag="K6T58_RS24095"
                     /old_locus_tag="K6T58_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165987.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1620..2048)
                     /locus_tag="K6T58_RS24100"
                     /old_locus_tag="K6T58_24020"
     CDS             complement(1620..2048)
                     /locus_tag="K6T58_RS24100"
                     /old_locus_tag="K6T58_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_000830439.1"
                     /translation="MKRSGGTRYMYLSALITVSAALTACTPKTSLEQHTRHYVYASDDG
                     FDPNFYTKKADTTRMMIPFFQQFREMGVKDKAAGVSAETAQQRIKEFHSEEFLQSLRTT
                     TTFAGRKYTNSDMPSPEKMKLLADTISAIYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2129..2548)
                     /locus_tag="K6T58_RS24105"
                     /old_locus_tag="K6T58_24025"
     CDS             complement(2129..2548)
                     /locus_tag="K6T58_RS24105"
                     /old_locus_tag="K6T58_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312322.1"
                     /translation="MAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTSQDGRREFETSE
                     LIRAYGELKRNETPERHTAGQAEKPHDQQTERILRELNELKQCLTLMLEDKQAQDTERR
                     RQEAEREQLQNEIAQLRQALEQERKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            2820..3578
                     /locus_tag="K6T58_RS24110"
                     /old_locus_tag="K6T58_24030"
     CDS             2820..3578
                     /locus_tag="K6T58_RS24110"
                     /old_locus_tag="K6T58_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001555316.1"
                     /translation="MSGIENSNQGPGWHDHNTPYGTIHSYDGDSHSGYGAHSGGNGGNR
                     HEGISDKTHISIPSTRNYNGYYMSMCVNNLKAHEGFKNTMYKDSEGNITVGIGHLLSTP
                     DMAASLPFTRTHTWHGHGDDMEKEVAASKKEITDAFNNFKKDSRQVPMNLHLSNDAVIG
                     LCISDVQTTEKGLRSLYSGYDGFSNSRKTALVDMGFNLGIPKLSRNFPNFNAAVNRGDW
                     ESAARESHRNLHQRGDQRNKDTADQLRDKH"
                     /name="hypothetical protein"
     gene            3661..4140
                     /locus_tag="K6T58_RS24115"
                     /old_locus_tag="K6T58_24035"
     CDS             3661..4140
                     /locus_tag="K6T58_RS24115"
                     /old_locus_tag="K6T58_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000595942.1"
                     /translation="MIKRLLFLYVALLFLSEGAYASVTVFIAPSWERNTDSVNEIYKYI
                     DDINSNNHVIDQAYVEPIRKELGVYRDYIQKKLINFNGKGVCKLSITTGSTGVKDIEPP
                     DVVLLNSLAENSSLDCKKLYREIQSIVDNDPSLLFPEKIKSNGLLSIDMIIVMGR"
                     /name="hypothetical protein"
     gene            4152..4283
                     /locus_tag="K6T58_RS24120"
                     /old_locus_tag="K6T58_24040"
     CDS             4152..4283
                     /locus_tag="K6T58_RS24120"
                     /old_locus_tag="K6T58_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368419.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071525901.1"
                     /translation="MRGLVFCALILILPGCQANYIRDVQGGTVAPSSSSAATGIEIR"
                     /name="colicin release lysis protein"
ORIGIN
        1 aacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
       61 cggtaagtgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      121 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcaa tttttgtgat
      181 gctcgtcagg ggggcggagc ctatggaaaa acgccagcgg cgcggccttt ttccctggtt
      241 ttgccttttt gctcacatgt tctttccggc attatcccct gattctgtgg ataactgtat
      301 tacagctttt gagtgagtag acaccgctcg ccgcagccga acgaccgagc gtagcgagtc
      361 agtgagcgag gaagcggaag agcgcctata ctggcatttt ctccttacgc atctgtgcgg
      421 catttcacac cggacatgcc gcatgattaa gccagtatac actccgctat cgctacgtga
      481 ctgggtcatg gctgcgcccc gacacccgct aaaacccgct gacgcgccct gacgggcttg
      541 tctgctcccg gcattcgctc acagacaagc tgtgaccgtc tccgggagct gcatatgtca
      601 gagggtttca ccgtcatccc cgaaacgtgc gaggcaactg cggtaaagct catcggcgtg
      661 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagttcgt tgagcttctc
      721 cagcagggtt aatgtctggc ttctgatgaa gcgggccatg ttcagggcgg ttttttcctg
      781 tttggtcact ggtctgatgt ctccgtgtaa gggaatttct gttcatggga gcatgataac
      841 tgtgacgcag gcgagagtga taaagctgag tgagcggtta gtcaggggcg caaacattcg
      901 aacgcggcca gtgcgatgca ggatagggaa aacgccgtaa aacgacgtcc ctttcggcaa
      961 tagcaatatc atggcacagc atatcaactg ataaataatc cctgtgcagt acagccgctg
     1021 ttgcataaaa tcgcgctctg cggcgagtgt tttttaatca gatattccgc tgatactccg
     1081 gaaaaacgcg agtctgttct catgtctgaa atgtccacac cccacgcaaa accaactttt
     1141 tgctgatttg ctttttaaat catgttgtta tggttttaaa aatgtatttt cttattcaga
     1201 tttatacaaa agcacgtcgt cgacgaccct atggcgacgc tatgacgacc ctgcttaggg
     1261 tcagtactga ctatctgtag aaaaaacagt taattaatgt aggggcgccc ctaggggcgc
     1321 gtcgcctttt tttacataac acccctgggg gcgctgccag gggtgtctgc ccggatatgt
     1381 agcaatgagc cagggattca cgaactgaaa atgtccgaaa atgtgcctca tgggcaccgt
     1441 atgcgcccaa acagatcgct attcagtttc gtgcatagtc atgcaacgcc ctgaaaacga
     1501 tcctgacgca tttttccggt tttcctgggg gtaaacattt ctttttgctg tgcctgcgtc
     1561 agaatcgcgc tcaacgcgtt ttaatggtgc gtacaattaa gggattatgg taaatccact
     1621 tactgtcttc cctcgtagcc atcgagataa atcgcagaaa tcgtgtcagc cagcagcttc
     1681 attttttccg gtgatggcat atcactgttc gtgtattttc tgcccgcaaa ggtggttgtg
     1741 gttctcagtg actgaaggaa ttcttcagag tggaattctt ttatgcgttg ctgtgccgtt
     1801 tctgctgata ctccggctgc tttgtctttc acccccatct ccctgaactg ctgaaagaac
     1861 ggaatcatca tccgtgtggt gtcagctttc ttggtgtaga agttaggatc gaacccatca
     1921 tctgaagcat aaacataatg ccgggtatgt tgttccaggc tggtttttgg ggtacaggct
     1981 gtgagggctg ctgacacagt tatcagggcg ctaagataca tatagcgtgt gccgccagaa
     2041 cgtttcatgc gagtctcctt accttaatgt atttattttc ggtgagttat tatcagaaga
     2101 ctatcttatc atcagtctct gacagcgttc agcgaccaaa caaccgggac cagaatcccc
     2161 gtttcctttc ctgctccagc gcctgcctga gctgggctat ctcattctgt agttgttccc
     2221 gttctgcttc ctggcgccgg cgctccgtat cctgggcttg tttatcctca agcatcagcg
     2281 tcaggcattg tttcagctcg ttcagctccc ggagaatgcg ttctgtctgc tgatcatgag
     2341 gtttttctgc ctgtcccgct gtgtgccttt ctggtgtctc attccgcttt aattcgccgt
     2401 atgcccggat cagttcacta gtttcaaact cccgtcgtcc gtcctggctt gtacggtaac
     2461 tgacaaggcc tgcgcgcata tcacggtaaa actgtgaacg cgatctgccc gtcattttca
     2521 gtgcgtcccg gagtgtgtgc catgccataa agtgacagtg tcccataggt gtctcatctc
     2581 atggtctcag taaaacatac tgaggccgga gaacggtaat gtttgtgctg gtttttgtgg
     2641 catctggcgg ggatggcaca tggtgtgagt ggctgttttt ttgaccgttt tcacaaaaat
     2701 gaaaatccac agggttgaca gggaaaacgc agcggcgtaa ttttaaactg tatataaaac
     2761 cagtggttat atatacagtt gtattgatcg ttgttttaaa tgtcaaagag gaaattaaga
     2821 tgagtggtat tgaaaattct aatcaaggtc cggggtggca tgatcacaat actccctatg
     2881 gaacaattca tagttatgat ggggacagcc atagcggtta tggtgctcat tctggtggta
     2941 atgggggaaa tcgccatgaa ggtatttctg ataagacaca tattagtata ccatctacca
     3001 gaaactataa tggctactat atgagcatgt gtgttaataa tttaaaagcc catgaggggt
     3061 tcaaaaacac catgtataag gatagcgaag gtaatattac tgttggtatt ggtcatttgc
     3121 tatctacgcc agatatggcg gcatctctcc cttttacaag aactcataca tggcatggtc
     3181 atggtgatga tatggaaaaa gaagttgcag catcaaaaaa agaaataacc gatgctttta
     3241 ataactttaa aaaagactct agacaagtac caatgaactt gcatttatca aatgatgctg
     3301 ttattggatt atgtatttcc gatgttcaga ctacagagaa aggcttgcgt tctttatatt
     3361 ctggttatga tggtttttct aactctagaa aaactgcctt agtggatatg ggatttaact
     3421 taggaattcc taagctatcg agaaactttc cgaactttaa tgctgctgtg aaccgtgggg
     3481 attgggagtc tgcagcacgt gaaagccatc gaaatcttca tcaacgaggc gatcagagaa
     3541 acaaggatac agcagatcag ttgcgggata aacattaata gaccataaca tatagatatg
     3601 agatgtattt catcttatat taaagatgaa atacatcaac ctataatggg aatggattat
     3661 atgatcaagc ggttattatt cttatatgtt gctttattgt tcttatcaga aggtgcttat
     3721 gcatctgtaa cagtattcat tgctccaagt tgggaaagga atacagattc agttaatgag
     3781 atatataaat atattgatga tataaatagt aataaccatg tcatcgatca agcatatgta
     3841 gaacctatta ggaaggagtt aggggtatat cgagattata tccagaaaaa actaattaac
     3901 tttaatggga agggagtttg taaattatcg ataacaacag gaagtacagg tgttaaagat
     3961 attgagcctc cagatgttgt tttgttaaac tcattggcgg aaaactcatc attagactgt
     4021 aaaaaactgt atagagaaat acaaagtatt gttgataacg atccgtcact tttatttcct
     4081 gagaaaataa agtcaaatgg tttgttatca attgatatga tcattgttat ggggagataa
     4141 aatacaattc tatgcgtggt ttggtttttt gtgcgctaat tttaatattg cccggctgtc
     4201 aggcaaatta tatcagggat gttcaaggag ggactgttgc gccatcttct agctcagcag
     4261 ctactggaat cgaaattagg taaaaagatt aaaggatctt cttgagatcc tttttttcta
     4321 cgcgtaatct gctgcctgta aacgaaaaaa cccacgttct caggcgtggg ttctttcgaa
     4381 ggttagaagt ttctcaggct ttctaacccg aggtaacagg gtgtacaaga ccgctgccac
     4441 caaatctgtc ctttcagtgt agccgcagtc ggtccttcac ttcaagaact acgtatcagc
     4501 aatttcttgt acatcctcta ccagtggctg ctgccagtgg cgt
//
