LOCUS       NZ_CP083261             2465 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Staphylococcus aureus strain HL25274 plasmid pHL25274_2, complete
            sequence.
ACCESSION   NZ_CP083261
VERSION     NZ_CP083261.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20667807
            Assembly: GCF_020034535.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Kim,J.-M., Chung,S.H. and Kim,J.-S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2021) Laboratory Medicine, Hallym university
            college of medicine, 150 Seongan-ro, Gangdong-gu, Seoul 05355,
            Republic of Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 08-JUL-2021
            Assembly Method                   :: SPAdes v. 3.14.0
            Assembly Name                     :: HL25274
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 333.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:26:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,890
            CDSs (total)                      :: 2,807
            Genes (coding)                    :: 2,717
            CDSs (with protein)               :: 2,717
            Genes (RNA)                       :: 83
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 45 of 90
            Pseudo Genes (incomplete)         :: 37 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083261.1.
            Bacteria and source DNA available from Department of clinical
            laboratory, Kangdong Sacred Hospital.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="HL25274"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pHL25274_2"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.53639137 N 127.13541226 E"
                     /collection_date="2020"
                     /collected_by="Kangdong Sacred Hospital"
     gene            1..735
                     /gene="erm(C)"
                     /locus_tag="K3A98_RS14420"
                     /old_locus_tag="K3A98_002863"
     ARG             2..732
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1183..1659
                     /locus_tag="K3A98_RS14425"
                     /old_locus_tag="K3A98_002864"
     CDS             1183..1659
                     /locus_tag="K3A98_RS14425"
                     /old_locus_tag="K3A98_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000664727.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            2354..2413
                     /gene="ermCL"
                     /locus_tag="K3A98_RS14430"
                     /old_locus_tag="K3A98_002865"
     CDS             2354..2413
                     /gene="ermCL"
                     /locus_tag="K3A98_RS14430"
                     /old_locus_tag="K3A98_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WP_002497446.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     ARG             2..735
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          1184..1669
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaacgaga aaaatataaa acacagtcaa aactttatta cttcaaaaca taatatagat
       61 aaaataatga caaatataag attaaatgaa catgataata tctttgaaat cggctcagga
      121 aaagggcatt ttacccttga attagtacag aggtgtaatt tcgtaactgc cattgaaata
      181 gaccataaat tatgcaaaac tacagaaaat aaacttgttg atcacgataa tttccaagtt
      241 ttaaacaagg atatattgca gtttaaattt cctaaaaacc aatcctataa aatatttggt
      301 aatatacctt ataacataag tacggatata atacgcaaaa ttgtttttga tagtatagct
      361 gatgagattt atttaatcgt ggaatacggg tttgctaaaa gattattaaa tacaaaacgc
      421 tcattggcat tatttttaat ggcagaagtt gatatttcta tattaagtat ggttccaaga
      481 gaatattttc atcctaaacc taaagtgaat agctcactta tcagattaaa tagaaaaaaa
      541 tcaagaatat cacacaaaga taaacagaag tataattatt tcgttatgaa atgggttaac
      601 aaagaataca agaaaatatt tacaaaaaat caatttaaca attccttaaa acatgcagga
      661 attgacgatt taaacaatat tagctttgaa caattcttat ctcttttcaa tagctataaa
      721 ttatttaata agtaagttaa gggatgcata aactgcatcc cttaacttgt ttttcgtgta
      781 cctatttttt gtgaatcgat tatgtctttt gcgcattcac ttcttttcta tataaatatg
      841 agcgaagcga ataagcgtcg gaaaagcagc aaaaagtttc ctttttgctg ttggagcatg
      901 ggggttcagg gggtgcagta tctgacgtca atgccgagcg aaagcgagcc gaagggtagc
      961 atttacgtta gataaccccc tgatatgctc cgacgcttta tatagaaaag aagattcaac
     1021 taggtaaaat cttaatatag gttgagatga taaggtttat aaggaatttg tttgttctaa
     1081 tttttcactc attttgttct aatttctttt aacaaatgtt cttttttttt tagaacagtt
     1141 atgatatagt tagaatagtt taaaataagg agtgagaaaa agatgaaaga aagatatgga
     1201 acagtctata aaggctctca gaggctcata gacgaagaaa gtggagaagt catagaggta
     1261 gacaagttat accgtaaaca aacgtctggt aacttcgtaa aggcatatat agtgcaatta
     1321 ataagtatgt tagatatgat tggcggaaaa aaacttaaaa tcgttaacta tatcctagat
     1381 aatgtccact taagtaacaa tacaatgata gctacaacaa gagaaatagc aaaagctaca
     1441 ggaacaagtc tacaaacagt aataacaaca cttaaaatct tagaagaagg aaatattata
     1501 aaaagaaaaa ctggagtatt aatgttaaac cctgaactac taatgagagg cgacgaccaa
     1561 aaacaaaaat acctcttact cgaatttggg aactttgagc aagaggcaaa tgaaaaacaa
     1621 gaaaatgcat tatctgatta ttattctttc aaggactagt ataacataaa atcgtctaca
     1681 aatagacaaa aaacctgcac gcttaatgta gatcaaaagc ttaacgcaaa tgaaatagat
     1741 tgacctccca ataacaccac gtagttattg ggaggtcaat ctatgaaatg cgattaagct
     1801 ttttctaatt cacataagcg tgcaggttta aagtacataa aaaatataat gaaaaaaagc
     1861 atcattatac taacgttata ccaacattat actctcatta tactaattgc ttattccaat
     1921 ttcctattgg ttggaaccaa caggcgttag tgtgttgttg agttggtact ttcatgggat
     1981 taatcccatg aaacccccaa ccaactcgcc aaagctttgg ctaacacaca cgccattcca
     2041 accaatagtt ttctcggcat aaagccatgc tctgacgctt aaatgcacta atgccttaaa
     2101 aaaacattaa agtctaacac actagactta tttacttcgt aattaagtcg ttaaaccgtg
     2161 tgctctacga ccaaaagtat aaaaccttta agaactttct tttttcttgt aaaaaaagaa
     2221 actagataaa tctctcatat cttttattca ataatcgcat cagattgcag tataaattta
     2281 acgatcactc atcatgttca tatttatcag agctcgtgct ataattatac taattttata
     2341 aggaggaaaa aatatgggca tttttagtat ttttgtaatc agcacagttc attatcaacc
     2401 aaacaaaaaa taagtggtta taatgaatcg ttaataagca aaattcatta taaaagaggg
     2461 ttata
//
