LOCUS       NZ_CP083260             3011 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Staphylococcus aureus strain HL25274 plasmid pHL25274_1, complete
            sequence.
ACCESSION   NZ_CP083260
VERSION     NZ_CP083260.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20667807
            Assembly: GCF_020034535.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Kim,J.-M., Chung,S.H. and Kim,J.-S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2021) Laboratory Medicine, Hallym university
            college of medicine, 150 Seongan-ro, Gangdong-gu, Seoul 05355,
            Republic of Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 08-JUL-2021
            Assembly Method                   :: SPAdes v. 3.14.0
            Assembly Name                     :: HL25274
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 333.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:26:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,890
            CDSs (total)                      :: 2,807
            Genes (coding)                    :: 2,717
            CDSs (with protein)               :: 2,717
            Genes (RNA)                       :: 83
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 45 of 90
            Pseudo Genes (incomplete)         :: 37 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083260.1.
            Bacteria and source DNA available from Department of clinical
            laboratory, Kangdong Sacred Hospital.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="HL25274"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pHL25274_1"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.53639137 N 127.13541226 E"
                     /collection_date="2020"
                     /collected_by="Kangdong Sacred Hospital"
     gene            1..582
                     /locus_tag="K3A98_RS14410"
                     /old_locus_tag="K3A98_002861"
     CDS             1..582
                     /locus_tag="K3A98_RS14410"
                     /old_locus_tag="K3A98_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991701.1"
                     /translation="MNAIQKSVSLRKVLVISLSFLTILSMVLNFNFKEAQAQNKNNIAD
                     KNIETLNEKEIEKELDYIYGKIIVLDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKNSSISPRAVGGNYKNSNDCFYSEVLNSYGELLTGNIIAAVFDDVKAKNIKSAAKKL
                     ARIGIKGNLAGIAATMVSKDVQCTLKYGWF"
                     /name="hypothetical protein"
     gene            complement(1565..2560)
                     /locus_tag="K3A98_RS14415"
                     /old_locus_tag="K3A98_002862"
     CDS             complement(1565..2560)
                     /locus_tag="K3A98_RS14415"
                     /old_locus_tag="K3A98_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199895.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAYNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EEVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQKALQVDYRPVANIKAI
                     KPNQKGDKDIESAIKETSKYSVKSSDFLTDNDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKITDEEAKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     Typing          complement(1331..1495)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.398"
                     /coverage="100.0"
                     /evalue="1.13e-79"
     Typing          complement(1566..2560)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.687"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaatgcaa ttcaaaaaag tgtatcacta agaaaagttt tagtaatatc tttaagtttt
       61 ttaactatac tttcaatggt cttgaatttt aattttaaag aagctcaagc ccaaaacaaa
      121 aacaacatcg cagataaaaa cattgaaact ttaaacgaaa aagaaatcga aaaagaacta
      181 gactacatat atggcaaaat tattgtttta gataaagacg gaaccgctaa agatgttaat
      241 ctagaaaatg ccaaatctag atatggatat gttcctgaag gtttccaaaa actaaagaac
      301 gacatagaga ataaaaagaa aaatagctca atatcaccta gagcagttgg tggaaactac
      361 aaaaatagta atgactgttt ctattctgaa gttttaaact catatggaga acttttaacc
      421 ggtaatataa tcgcagcagt ttttgatgat gtaaaagcca aaaacataaa gagtgcagca
      481 aaaaaattag ccagaattgg cataaaagga aatttagctg gaattgcagc tactatggtt
      541 tcaaaggatg tacaatgtac tctgaaatac ggatggttct aaatatataa tttattccaa
      601 ttgctttatt gacgttgagc ctcggaaccc ttaacaatcc ctaaacttgt cgaatcgtcg
      661 gcttaatagc tcacgctatg ccgacattcg tcttcaagtt tagttaaggg ttcttctcaa
      721 catcaataaa ttttctcggc ataaatgcat gtttgattat gcgtgtaact atcccttaaa
      781 taaaacatta attgtgcact gatttactaa ttattatccc ttgctttcat tcacactata
      841 atcaacacct taattctaaa catctgaagt aatcataatt tttaaaccta ataaaattcg
      901 tgactatctt agttaaactc tgtttaatta agatttttta tttttcgtta tccaagaggc
      961 gcatttacat acttaaaaat gcgtcgagct aaaaccaaaa tttacgtgta ataatcgaat
     1021 atacttattt tgcattttaa gggggtattt agaacgaact ttaaaaatat atagttttat
     1081 acctgtatta tcttatttgt ttcttaaaac gcaaatatga gccaaataaa tatattctga
     1141 ttttataatc aaaaattttt tataatcaaa aattcgatca aacccagtgt cgatttttcg
     1201 gacactgccc atttacatgc aaattaaaaa ttagcataaa atcctttaaa acttccccca
     1261 tgtttttcta aattcctgca gggcgctttt tatcaaaaat cgagaatttt taatttttct
     1321 ctaagaactc cgtaagagca taaggagaat ttgctatcat tcaaaaatga taggtctggc
     1381 aaagccagac attccaaggg ttttaagtaa ttttaaagtg atatcataaa aagctgtagt
     1441 gtcacacttt tgtgacacaa tatattgcgt ttgtcacagt tttgtgacac tacatacagc
     1501 atttgtcaca aaaataagac agtacttttt tacaaaaaat caataaaaaa gacatcagtc
     1561 atcactaatg tcttaaataa taattttgtt tttcaaaatt ccaaattgcc gtaattgaat
     1621 gtgcttttgc ttcttcatct gttattttat cttcatcact tgtattaatt aaattaccat
     1681 cttcagcatc gtctaagttt aaaattttat gcttttgttt aagcaatcca ccataactta
     1741 acatacgttt tcgatataaa cctttttcta aatcacttac aatttcttga tttttttcgt
     1801 cattgtcagt taaaaaatca gatgacttaa ccgaatattt tgatgtctct ttgattgccg
     1861 attcaatatc tttatcgcct ttttgattag gtttaattgc tttaatattt gcaacaggtc
     1921 gataatcaac ttgtaatgct ttttgccata aattaaccca ttcttcttga gttatataat
     1981 tctctttttt tctaaaatat gcattttcaa cacataacaa aacgtgcata tgctgattat
     2041 aactaccatc atttttatta acggtaactt ctgttgaacg cataaatcca acaagatttt
     2101 gtttaacctt tttatatcta ctcaacctat caaatgcttt agttagatgc ttcaaacttt
     2161 gttctaaagt atctccatct atcgcatttt tggttgaaag tgttaaaaac aaccaacgtg
     2221 cttttggctt ttccttaatt acttcttcaa tcactttttg agcttgataa ctatttttca
     2281 tagcacgcct ccaattacaa actggacata gctttgattt acaaaaccac gtcttatgta
     2341 atttcaaata accttcatcg gtcggcttga attctaagac gttaccacat tgttttacgt
     2401 tataagcctt ttttattttt aaaatttcaa gtatatctgc atagcttaca ttatctattt
     2461 tcttttctct ccatgggcgt tgtttcccac ttttcgtcat atcttttaac gttttattat
     2521 cttgattttc ggttatactt ctagtagtat tatattgcat ataaaaagac ccccagtatt
     2581 atttaagatt cgacacctta ataatataac tggggttttt tattgtcaaa ttttcctttc
     2641 acgcacaaaa acacgtttaa acgctgtaat atcaacagtt acagctatat atataaaatc
     2701 gagttatttc tatatagtat caagacaaga agaaactcgt tttcaactcg tttcaaaaac
     2761 aatctatttc tttttctctt tatagccctc taaacctaaa taaatcaatg tgaaaaccaa
     2821 cgaataaata aagctttcta aaaagtcaaa aggattatta aataaaatcg aattaacaaa
     2881 attaaatatt aaaaatacgg acaataaaac aattaaaaaa taaataaact tattcatatt
     2941 aaactccttt gtttttatat gttaatatta tattggattc atttaaataa caaaacaagg
     3001 gggcatttat a
//
