LOCUS       NZ_CP083166             9463 bp    DNA     circular CON 11-APR-2024
DEFINITION  Bacillus thuringiensis strain ABTS-1857 plasmid pCH_181-j, complete
            sequence.
ACCESSION   NZ_CP083166
VERSION     NZ_CP083166.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214332
            Assembly: GCF_020809245.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x, 40x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809245.1-RS_2024_04_10
            Annotation Date                   :: 04/10/2024 23:39:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,957
            CDSs (total)                      :: 6,806
            Genes (coding)                    :: 6,416
            CDSs (with protein)               :: 6,416
            Genes (RNA)                       :: 151
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 175 of 390
            Pseudo Genes (incomplete)         :: 226 of 390
            Pseudo Genes (internal stop)      :: 99 of 390
            Pseudo Genes (multiple problems)  :: 87 of 390
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083166.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="ABTS-1857"
                     /isolation_source="Biopesticide XenTari"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_181-j"
                     /collection_date="2017"
     gene            22..552
                     /locus_tag="K8Z20_RS34570"
                     /old_locus_tag="K8Z20_34175"
     CDS             22..552
                     /locus_tag="K8Z20_RS34570"
                     /old_locus_tag="K8Z20_34175"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="WP_021729417.1"
                     /translation="MDYYIYAFSLIVTKGLLKTANEEELAGILAHELGHLKYGDSKRSV
                     MAHTLNTAGTVATWILVAMATILSLLSNRVPIVAVLAALAAVVLKAMQVVLHRVADLSF
                     RVVGRKEEFRADAYAVELGMGAGLLSFLEKIEGTEGSPKGIWARLEATHPPVAFRIEQI
                     EISLEEQQKETIL"
                     /name="M48 family metalloprotease"
     gene            complement(2558..3286)
                     /locus_tag="K8Z20_RS34575"
                     /old_locus_tag="K8Z20_34180"
     CDS             complement(2558..3286)
                     /locus_tag="K8Z20_RS34575"
                     /old_locus_tag="K8Z20_34180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021728966.1"
                     /translation="MKIKQISAVLSGALLIGSLSPSISQAATQKETPVKTEQVQEKQNN
                     QNNNFIPIQTNEQFNQTLNTTKTFTPYVKVNAEGLLYINATADEIGITNEEYQNFLNGL
                     NLENEYIKKGYFTLEKNEQGNPGKLSTTQALNSYVNNDLSQFKELQSSSAGFYGWGFGL
                     KLSNSETQSLAEAMAWGSAAIWVAAELAAAGYVTIPASVPLGLIAAIVGLGAATLAVVN
                     KGKGVTVGITWTGHPHIHRN"
                     /name="hypothetical protein"
     gene            complement(3493..3633)
                     /locus_tag="K8Z20_RS34580"
                     /old_locus_tag="K8Z20_34185"
     CDS             complement(3493..3633)
                     /locus_tag="K8Z20_RS34580"
                     /old_locus_tag="K8Z20_34185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023901786.1"
                     /translation="MKKTTFYLLGIITAFTLTLGNTNTSKETQISAEKNIIQYSHGNTG
                     G"
                     /name="hypothetical protein"
     gene            complement(3630..4724)
                     /locus_tag="K8Z20_RS34585"
                     /old_locus_tag="K8Z20_34190"
     CDS             complement(3630..4724)
                     /locus_tag="K8Z20_RS34585"
                     /old_locus_tag="K8Z20_34190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap family tetratricopeptide repeat protein"
                     /protein_id="WP_050062578.1"
                     /translation="MDVQTKNNEQITNMLNDWYIEIRARHLGNAHKLRLEIDKKIHNIE
                     EDQNLLLYYSLLDFRHQYLMDHLSIGKNSFDKIESFNTPTDNLLSYYYFFFKAIHATSI
                     GNYNLARKYYDKAEIKLKEIPDQLEHAEFYYKLSTFSCHNQQYVPAIKQASKAKEIFSK
                     YKGYELNIGYCNNLSGLSCTHLQEYELAEEYFISAMDIFQKEKEEQAILYVRHNLGFMY
                     AKQNLSELAIRYLSEVIQKSPNNYRAILIEACEHSKLNNKNEASELFEQGLQISTELEN
                     EEYQHHFKILKAINEEIPGENLEKLVIAGNKYFEQENIYEYVHEYNEKLALKFYREDNH
                     EKASKYFYLSSKANQKKQDKEALK"
                     /name="Rap family tetratricopeptide repeat protein"
     gene            5179..5835
                     /locus_tag="K8Z20_RS34590"
                     /old_locus_tag="K8Z20_34195"
     CDS             5179..5835
                     /locus_tag="K8Z20_RS34590"
                     /old_locus_tag="K8Z20_34195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021729703.1"
                     /translation="MKKIVDFSEVEKNARKRDLEIERKKHENHYTKEQEEGLGQALETL
                     QNLTGAEYYVGRKRDPFAQVEFSQIIQANVRYLIKIGYLTNAERLLLLDLSCYVEVGTH
                     VLVEKDVDSIDRDVINNASVTYLAEETYRTRHGLSKLMKNLKDKGILACAESGFSDESG
                     RVCTKRTWLVNPNIICASRKTSIDRVTKHIFKGILKNFTLDSDSKKIHNLPVYFF"
                     /name="hypothetical protein"
     gene            6861..7499
                     /locus_tag="K8Z20_RS34595"
                     /old_locus_tag="K8Z20_34200"
     CDS             6861..7499
                     /locus_tag="K8Z20_RS34595"
                     /old_locus_tag="K8Z20_34200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001996445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021729419.1"
                     /translation="MAITKRDIEIVEWVNLHGFVLAEQVQEFFGLGKTVVYRRLKEMVE
                     QGVLKRERILYDYGNVYWVTKDGVELCESEMAAGKKPSVNNFVHDKKLVDLSVKLLKKY
                     EGSSWITARQIKSRLVRKASEKDKFKALAMRVADGILIVNGKKYAVELELSLKNRTRVK
                     QIIGDYAERIAKGQYVSVIYVVEKESIAKVLREEMNQTVVSDRFQIMTF"
                     /name="hypothetical protein"
     gene            7562..9454
                     /locus_tag="K8Z20_RS34600"
                     /old_locus_tag="K8Z20_34205"
     CDS             7562..9454
                     /locus_tag="K8Z20_RS34600"
                     /old_locus_tag="K8Z20_34205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="WP_021729418.1"
                     /translation="MATNQYEENRNSLEWIRFAAVICFTSGYLIAIIFKKIGELAQVGK
                     LEKRIVFWIAFGFSILWVLNDFQTLLASSVVSFVISFVENYFQVLPMELKHFVFAIFPE
                     HNTLLAFLVNGVKTAGLVIAIENVFKWATRVDESTVQNSPSLEREKQEKSLANKVIVNL
                     KEKQQEKSYQKEMDRGEIPKGVRLGRTMTGGQEIILTPDVLKKHIGLVGTTGSGKTITI
                     KTFLEYAILKKQAGIFIDAKGDMKLAEEIRKFAEKHKRPFYHFNTDGVGMHYNPLAVGT
                     PTELTDKILTLSDWSEEHYKLSSQEFLQYLFRVFEARNVKATLTSVSQYCDADALIGLI
                     TSQSEIEVHEEAPKKVVVSDLSDTDSLVLFDDEKIDVSKSDPSESIFKRDEVFNLIPKI
                     KRIDKKAIEGLASRVSILAEGDFRHLFQEEVGNTIALHQVLEERGMVIFTLDSLRYPEG
                     ARQLGKMIVNDLKTNVSSHMKHREGEHVTVAIDEFNVLVTQQVIDVINKSRGAGFEALL
                     AFQSLADIETVSEHLRKQIMQNCNTLIVQAQNDPSDAEELAKAIGTADSLDFTYQVDMA
                     QGVTGLGSMRNVKNFVFHPDEIKALQTGEALVKIKQGDKLVRKKIKVRMVKWED"
                     /name="type IV secretion system DNA-binding
                     domain-containing protein"
     Typing          5180..5835
                     /name="rep_cluster_1767"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.043"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7563..9427
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="96.141"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatgttaaca gcttttgtga attggattat tatatatatg cttttagcct tattgtgaca
       61 aagggattat taaaaacagc aaatgaggaa gagcttgcag ggattcttgc tcatgaactt
      121 ggtcatttga aatatgggga tagtaagcgt tctgtcatgg cgcatacgtt gaatacagcg
      181 ggtacagtag ctacatggat attagtggct atggcaacga ttttatctct tctctcgaat
      241 cgtgttccga ttgtggctgt attagcggcg cttgcagcgg ttgtattaaa agccatgcaa
      301 gtcgttttac atcgagttgc tgatttatcg tttcgagttg ttggaagaaa agaagaattt
      361 cgagcggacg cgtatgcagt tgaattagga atgggagcag gtttgttgtc ctttttagaa
      421 aaaatagaag gaactgaagg ttctccaaaa gggatctggg cgcgtttaga agcgactcat
      481 ccacctgtag catttcgaat tgaacagata gagatttcgt tagaagaaca acagaaagaa
      541 acgatattat aaaggaattt atgatataat aaatattcat cactaactaa ctttaagggc
      601 ttcatccttt taaaaatctc tgtatatgtg tgcatataca gagatttttt gcgtttatgt
      661 acttgtaagt atggtaagat tgataagact acatttgaaa atgtgggttc aagttgctgt
      721 ttgactgaat tgcaccaaaa aaccaattcc attacacggg aattggtttt ttgggtgaaa
      781 tttagtatat ttattgctag cctaaaggag attcgtttct ttctaattta gtataactta
      841 aatagaaaga aaattcaaaa ataagagaat gaatatttac atattttcaa attaaacaac
      901 aaaaatgtaa ggatatgggt tataatgagt taagaaacat tctcctgttc ctatgtttct
      961 attgattgtt atttttatct gtttttggtg caaagtctgt agatagattt tgcacccccc
     1021 ttcatttttt atatttatat acggatatta agattttgtg tataatgatt atgtctgtag
     1081 tttcttttaa ttcgtttata taaagggcaa ttcctgtttt tttaaggaat tgcccttttg
     1141 tgtgcctttt ataaatcaat acagtatatg tgacaataga tgagaaggtt cttattatat
     1201 ggaaatatac aagttaatac aaaaagaaat agcatgtttt cttttttaaa aaacaaagat
     1261 tcttttatat tttgtttatt ttcaatgaga catactcaaa ttttataata agagcatatt
     1321 tttataaagt atcttcttct tttgaacaga atagaagaat gcaagagatg actttttgct
     1381 ggatataagt gatacagata gtttgtatat ttgtatcact tatccattta aaaataaaag
     1441 aataaattaa tacataattt tgaattaata caaaaaaatg taaaaacaca gatggtaggt
     1501 agtaaaatcc ttttctatgt ttctgttgat aaaattccac ttttagaatc attagacaaa
     1561 agtgttaata tataggttat aataaagagg taataagttc agttttctga gtttaatatg
     1621 tgtatttaaa tgagcgatcg gttttgtttg ttttgtagga aaatgagaag tctttttaaa
     1681 aagacttctc atttttatgg atggaaggag tgatatatat tataaactat tattcttatc
     1741 atcaaatgtt tttaattttt atgacggtta tagctatatt tttatatatt ctatatgtga
     1801 acagaccgac ttcaaaaaat aaatggaatc ttttgatcgt agtgttttta ttttttttta
     1861 ttaattattt atagtttttt tgtagtaaag ctaaagtaaa cttttaaata ttagtttact
     1921 ttcaaagttt tgattttata ggctcgtaag aggtttttag aaaaatagat atttccgtaa
     1981 ggaaagtata cattcagaac acgagaatga aaggatttcc acgcccgacc tttgccccct
     2041 agtcttcgca gtacggagtg gcgcactggc ataggaaaca caggttggag gctcgatgta
     2101 aagggcatga agcgtagcgg aatgggcata gccgaaccct ttatatggag accgcctgtg
     2161 ttagcctatt cgggcaagcc agtccggttt gcgaagtggg ggtaaagaga aggagggcat
     2221 aaagtggata aaaaacaaaa aatctaagct tgtaagctta gattttttgt tttttatgta
     2281 gcatttaata ataagaaaag ccatgtaatg aaaaatgcta caactaaggg aattattttt
     2341 aaaaagatta ataggttttt atttaagtag gtgtattttt ttttgttgaa aatatattct
     2401 aggctaactg ttatacaaat aaggatcagt ataattaaaa tagatttctt gaacagtaaa
     2461 ttagatacca aattatataa actaagacct aagagtacga ttatagatgc aaataatcga
     2521 atgttttttt tcataaatcg ttctcctttt tcatttatta gttacggtgt atgtgtggat
     2581 gtcctgtcca tgtaattcca actgtgacgc cttttccttt gttaacaaca gctaatgtag
     2641 cagctccaag tccaactata gctgcaatta atcccaatgg tactgaagct ggtattgtta
     2701 catatccagc tgctgctaat tcggctgcaa cccaaattgc tgcactaccc catgccattg
     2761 cttcagctag tgattgagtt tcagaattac ttagttttaa tccgaatccc catccataaa
     2821 aaccagctga agaagattgt aattctttga attgacttag gtcattatta acatatgaat
     2881 ttagtgcttg tgttgtggaa agttttccag ggtttccttg ttcatttttt tctagagtga
     2941 aatatccttt tttaatgtat tcattttcta agtttaatcc atttaagaag ttttgatatt
     3001 cctcatttgt tataccaatt tcatctgctg ttgcattaat atataaaagt ccttctgcat
     3061 ttacttttac atatggagta aatgtttttg ttgtgttaag tgtttgatta aattgttcgt
     3121 ttgtttgaat gggaataaag ttattgtttt gattgttttg tttttcttgt acttgttctg
     3181 tttttactgg tgtttctttt tgtgttgcgg cttgcgaaat gctaggtgat aaactaccga
     3241 ttaacaaagc tccacttaaa actgctgaaa tttgttttat tttcattata aaaccacctt
     3301 ttttgtaatt tggaatatat tagattccta ttacatttta tttcatattt ataaataatg
     3361 taatattatg ttaaaaattg taatattatg cttatttatc aaaaccttga aacatgtgat
     3421 ttttttataa aaaaaacctt ctgaagtaga aggctttttt gtttgtatgc ttaaagtttt
     3481 aaaatcaatt agttaaccac cagtgtttcc atgtgaatat tgtattatgt ttttttcagc
     3541 ggatatttga gtttcttttg atgtatttgt gtttcctaag gttaaggtga aagcagttat
     3601 gatccctagt aagtaaaaag ttgttttttt cattttagtg cctccttatc ttgttttttt
     3661 tggttagctt ttgaacttag gtaaaagtat ttgcttgctt tttcatgatt atcttcacga
     3721 taaaatttta atgctaattt ttcattatat tcgtgaacat actcatatat attttcttgt
     3781 tcgaaatatt tgttacctgc tattactagt ttttctaaat tttctccagg gatttcttca
     3841 tttattgctt ttaaaatttt gaagtgatgt tgatattctt cattttcaag ttctgtacta
     3901 atttgtaggc cttgttcaaa tagttctgat gcttcgttct tattatttag ttttgaatgt
     3961 tcacatgctt caattaagat tgctctgtag ttatttggtg atttttgaat aacttcggat
     4021 aaataacgaa ttgctaattc tgataaattt tgttttgcat acataaatcc taggttatga
     4081 cgaacatata aaatagcttg ttcttctttt tctttttgga atatatccat tgctgatata
     4141 aagtattctt cagctaattc gtattcttgt agatgtgtgc atgataatcc agaaaggtta
     4201 ttacaataac ctatgtttaa ttcatatcct ttgtatttag aaaagatttc ttttgcttta
     4261 gatgcttgtt taatggctgg aacatattgt tgattatggc aactgaatgt cgaaagttta
     4321 taataaaatt cagcatgttc taattggtcc ggtatttctt ttaattttat ttcagctttg
     4381 tcatagtatt ttcttgctaa gttgtagttt ccgatgctag ttgcgtgtat ggctttaaaa
     4441 aagaaataat agtatgagag aagattatct gtaggtgtgt taaaagattc aattttgtcg
     4501 aagctatttt taccaatact taagtgatcc attagatatt gatggcgaaa atctaatagt
     4561 gaatagtata aaagtagatt ttggtcttct tctatgttgt ggattttttt atcaatttct
     4621 aaccttaatt tatgtgcatt acctaaatgt ctagcacgaa tttcgatata ccagtcattt
     4681 aacatgtttg tgatttgttc attatttttt gtttgaacgt ccacaaagta gccccctttt
     4741 tacaaatgta tattatttcc actcatttta tcatgtctcg acatatattt actttattaa
     4801 atttgatata tattattttt aatattcagt aaattacggt tgaaaattat acccattaac
     4861 aaagaaacct taagtattaa ggtttctttg ttaatgggta taaataaggt atttgtctta
     4921 ttgacatttt taggatatca aaaatgtatg aatttgtata tatttctttt tgtgaatata
     4981 aaaagtcaga caagatacaa caaaaaaagc cattcatgct ataattgagt tgcttagaca
     5041 acaactatag tgaaggggct ttttttgtat cttaaaagat tattagagta tcagactcaa
     5101 agaaaataat tctaattaca taataacaaa ataaaagtct ctttgctagt tgtaaaaact
     5161 agaagtggag agttatttat gaaaaaaata gtggattttt cagaagttga gaaaaatgcg
     5221 agaaaacgtg atttagagat tgaacggaag aaacatgaga atcattatac gaaagagcaa
     5281 gaagaaggat taggacaagc tcttgaaaca ttacaaaatc ttacgggtgc tgagtattat
     5341 gtaggacgta aacgagatcc atttgctcag gtggaatttt cccaaatcat tcaagctaat
     5401 gtacgatatt taattaagat tggatattta actaatgctg aacgtttgtt acttttagat
     5461 ttgagttgtt atgtagaggt tggaacgcat gttttagttg aaaaagatgt tgatagtatt
     5521 gatagagatg taattaataa tgcttcggtt acttatcttg ctgaggagac ttatagaact
     5581 aggcatggct taagtaagtt gatgaagaat ttaaaagata aaggaatatt agcttgtgcc
     5641 gaaagtggct tttctgatga atcaggaaga gtttgtacaa aacgaacttg gttagtaaat
     5701 cctaatatta tttgtgcttc aagaaaaaca tcaattgata gagttacgaa acatattttt
     5761 aaaggtattt tgaagaattt cacattagat tcagattcta aaaaaataca taatttaccg
     5821 gtttattttt tttgagagat actctagtat ttctcttttt tttatgttag aaccgaacaa
     5881 actttcgtac taaaatttgt acactagtgt gcaaaaattt gtacactccc cccctcatga
     5941 acccttgata acactgactt tttttttttt ccttcttact cttatatata tatgctcttt
     6001 cgttccatat ttttgttgtt ttttgaggtt aagatacgtt atatttccac gctatattta
     6061 gataaaaatc cgaaggattg cccctttttg tagtttccgt gcttcgctat cgtactggct
     6121 agccaaaata ggctatcatg gggattctca ttgtaaaggg tttggctacg cccgttccgc
     6181 tatgctacac gccctttaca atgagcctcc accccatgac ttatttgcca gtgcgccagc
     6241 tctggccgct tagcatggga aactacaagg ggcgttcctg attaaataat agatttcaaa
     6301 acgataaaaa taaaaataac aaaacctttt gaaaatcaca aaatataaag attgaaaaat
     6361 tataaaagaa acaaacattt gatttcatca aacgtttgtt taaaaatagc cgcgctatgg
     6421 cgcggctatt tgcaaagacc tatgaaaaaa catatagaaa aaattatgat atatagttcc
     6481 atacttattt tccatagttt gcatgtagac tgaagttgca tttagacgct gtatatagcc
     6541 gattccccgc ccctctcctt cttttcttcg ctattgctac gaacctcctg accactcaca
     6601 tactacgtct acatgtgagt ggtcaggagg tcagaaggag aggggcgggg aatatgagaa
     6661 ttgagtaaga gaaatagagt agaagaataa gagagaaaga gagaagaata agtgtaaaat
     6721 agaaagaagg gatagacgaa tagtaagtat ggaaatagat atggaagacg atggtaagaa
     6781 tatggtaaaa taataggtgg taaagataag agattggaag agtagatgta tagaaggata
     6841 gaagagtagg agagggaaat atggcaatta cgaagagaga tatagagata gtagagtggg
     6901 taaatttgca tgggtttgta ttggcagaac aggtacagga gttttttggt ttgggaaaga
     6961 cggttgttta taggagatta aaagagatgg tagaacaggg ggtattgaag agggagagga
     7021 ttttatatga ttatggaaat gtgtattggg taacgaaaga tggtgtggaa ttgtgtgaga
     7081 gtgagatggc agcggggaaa aagccgagtg taaacaattt tgtgcatgat aagaaattag
     7141 tggatttgtc ggtaaagttg ttaaaaaaat atgagggatc aagttggata acagcaagac
     7201 agattaagtc tagattagtt cggaaggcaa gtgaaaaaga taagtttaaa gcgttagcta
     7261 tgagagtagc agatggaata ttgattgtga atggaaagaa gtatgcagtg gagttagagt
     7321 tgtcattgaa gaatcgaaca agagtgaaac agattatagg agattatgca gagagaattg
     7381 caaaagggca gtatgtgagt gtgatttatg ttgtagagaa agaaagcatt gcaaaagtat
     7441 tacgggaaga aatgaatcaa acagttgtat cagatagatt tcaaattatg acattttaat
     7501 ttaggttaac cttagttaac taggttattt aaggttaact tttaaagaga ggaatataaa
     7561 tatggcaact aatcagtatg aagaaaatcg aaattcatta gaatggattc gttttgcagc
     7621 agttatatgt tttacaagtg gttaccttat tgcaattatt tttaaaaaga taggtgaatt
     7681 agcacaagta gggaaattag agaaacgaat tgtattttgg attgcatttg gattttcgat
     7741 tttatgggta ttgaatgatt ttcagacatt gttagcatct agtgttgtgt catttgtgat
     7801 ttcatttgta gaaaattatt ttcaagtatt gccaatggaa ctgaagcatt ttgtatttgc
     7861 tatttttcca gagcataata cgttgttagc ttttttagtg aatggtgtta aaacagcggg
     7921 gttagtaatt gcgattgaaa atgtgtttaa atgggcaact agagttgatg aatcaactgt
     7981 acaaaatagt ccctctttag agcgagagaa acaagaaaag agtttggcca ataaggttat
     8041 tgtgaattta aaagaaaaac aacaagaaaa aagctatcaa aaagaaatgg atcgtgggga
     8101 aattccaaaa ggtgtacgtt taggaagaac tatgactggc ggtcaagaaa tcatactaac
     8161 accagatgtt ttaaagaaac atattggatt agttggaaca actggctcgg gtaaaacgat
     8221 aacgattaaa acatttttgg aatatgcaat tttaaagaag caagcaggga tttttattga
     8281 tgcaaaagga gatatgaaac ttgcggaaga aattcggaag tttgctgaaa agcataaacg
     8341 tcctttttat catttcaata cagatggagt tgggatgcac tataatcctt tggctgtagg
     8401 gactccaaca gaattaacag ataaaatttt aacgttgtct gattggtctg aagagcatta
     8461 taagttatct tctcaggaat ttttacaata tttgttccgt gtttttgaag ctaggaatgt
     8521 aaaagcaact ttaacaagcg tctctcaata ttgtgatgct gatgctttaa ttggattaat
     8581 tacaagccaa tcagaaatag aagtacatga agaagcgcct aaaaaggttg ttgttagcga
     8641 tttaagtgat acagatagtt tagtgttgtt tgatgatgaa aaaattgatg taagcaaatc
     8701 tgatccttct gagagtattt tcaaacgtga tgaagttttc aatttgattc caaaaattaa
     8761 gcggattgat aaaaaggcaa ttgaaggttt ggccagtcgt gtttctattt tggctgaagg
     8821 agattttcga catttgttcc aggaagaagt gggaaataca atcgcattac atcaagtgtt
     8881 agaagaaaga gggatggtta tttttacatt ggatagtttg cgatatccgg aaggcgctcg
     8941 acaacttgga aaaatgattg tgaatgattt aaaaacgaat gtgagtagtc atatgaaaca
     9001 cagagaaggt gaacatgtaa cagttgctat tgatgaattt aatgtacttg ttacgcagca
     9061 agttattgat gtcattaata aatctcgtgg tgcaggattt gaggcattgc ttgcttttca
     9121 atccttagca gatatagaaa cagtgagtga gcatttacga aaacaaatca tgcaaaattg
     9181 taatacttta attgttcaag cacagaatga tccaagtgat gcagaagaat tggcaaaggc
     9241 aattggtaca gctgatagtt tagattttac ttatcaagtg gatatggctc agggcgtaac
     9301 gggattggga agtatgcgaa atgtgaaaaa ttttgtgttc catccagatg aaattaaagc
     9361 attacagact ggggaagctt tggtgaaaat aaagcaaggt gataaactag taaggaaaaa
     9421 aattaaggta agaatggtaa aatgggagga ttaattacgt gtt
//
