LOCUS       NZ_CP083165            10657 bp    DNA     circular CON 11-APR-2024
DEFINITION  Bacillus thuringiensis strain ABTS-1857 plasmid pCH_181-i, complete
            sequence.
ACCESSION   NZ_CP083165
VERSION     NZ_CP083165.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214332
            Assembly: GCF_020809245.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x, 40x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809245.1-RS_2024_04_10
            Annotation Date                   :: 04/10/2024 23:39:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,957
            CDSs (total)                      :: 6,806
            Genes (coding)                    :: 6,416
            CDSs (with protein)               :: 6,416
            Genes (RNA)                       :: 151
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 175 of 390
            Pseudo Genes (incomplete)         :: 226 of 390
            Pseudo Genes (internal stop)      :: 99 of 390
            Pseudo Genes (multiple problems)  :: 87 of 390
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083165.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="ABTS-1857"
                     /isolation_source="Biopesticide XenTari"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_181-i"
                     /collection_date="2017"
     gene            join(10579..10657,1..185)
                     /locus_tag="K8Z20_RS34515"
                     /old_locus_tag="K8Z20_34120"
     CDS             join(10579..10657,1..185)
                     /locus_tag="K8Z20_RS34515"
                     /old_locus_tag="K8Z20_34120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874968.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPSELIKIGISIDDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            complement(1045..1212)
                     /locus_tag="K8Z20_RS34520"
                     /old_locus_tag="K8Z20_34125"
     CDS             complement(1045..1212)
                     /locus_tag="K8Z20_RS34520"
                     /old_locus_tag="K8Z20_34125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011279307.1"
                     /translation="MYKKGDIVKHTCWECYNEEMKIYRISKSKVKDGEDVYYSYKCWVK
                     CEKCGAVDDV"
                     /name="hypothetical protein"
     gene            complement(1215..2258)
                     /locus_tag="K8Z20_RS34525"
                     /old_locus_tag="K8Z20_34130"
     CDS             complement(1215..2258)
                     /locus_tag="K8Z20_RS34525"
                     /old_locus_tag="K8Z20_34130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162903.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_021727501.1"
                     /translation="MTKKNQDEITGKLQPKKKQNIRIYEFIEKKMSESGRELFKSCSTF
                     NEFVATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADMVKGEIDRFNKAFKKLFDRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVDKSYFKKKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQDVFDVFYLALKGRQLIVYAG
                     LLKDYAKKYENGELDKYKSIDKNEYYYRLIAMWNKDLMKFEQEYQELTESEKQEYNGHL
                     IDEMEVE"
                     /name="protein rep"
     gene            complement(3028..3600)
                     /locus_tag="K8Z20_RS34530"
                     /old_locus_tag="K8Z20_34135"
     CDS             complement(3028..3600)
                     /locus_tag="K8Z20_RS34530"
                     /old_locus_tag="K8Z20_34135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006097509.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_011279306.1"
                     /translation="MSKKEHLIDVQPIRSKEQLQDMKWALKRHCTDRDYILFVIGINTG
                     LRVSDLLNLETSMILELKKKRRKELKIREGKTKKERMVNITSIFEEVYLYAAGLESKWL
                     FPSRKGDRPISKIQVYRQLQKAGDFAGVESIGTHTMRKTFGYWFYKQTKDVAMLQEILN
                     HSAPKITLKYIGINKEEKDNVLDTFCL"
                     /name="tyrosine-type recombinase/integrase"
     gene            4188..4472
                     /locus_tag="K8Z20_RS34535"
                     /old_locus_tag="K8Z20_34140"
     CDS             4188..4472
                     /locus_tag="K8Z20_RS34535"
                     /old_locus_tag="K8Z20_34140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126403.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_021727500.1"
                     /translation="MTANTRHMETQKIERKVTKIGNSLGLTLPQEFYTTLNIKQGDVVS
                     LEINEENEQIVIKKSKKISMPEGLNPKFLESLNRTINKFDGAFKDLVNR"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            4510..4911
                     /locus_tag="K8Z20_RS34540"
                     /old_locus_tag="K8Z20_34145"
     CDS             4510..4911
                     /locus_tag="K8Z20_RS34540"
                     /old_locus_tag="K8Z20_34145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158318.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WP_021727499.1"
                     /translation="MTIYITETQAIFINETLIDMYSPNEQRGVKDTGLLQSAIYRPQQT
                     VAQEDAYPTIFHKATALFESLAKNHAFYNANKRTALACLEMFLLYNEYELKISEQEASD
                     FTVNVVEQRLSFDEILNVIKENSSKLPSN"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(4992..6266)
                     /gene="ltrA"
                     /locus_tag="K8Z20_RS34545"
                     /old_locus_tag="K8Z20_34150"
     CDS             complement(4992..6266)
                     /gene="ltrA"
                     /locus_tag="K8Z20_RS34545"
                     /old_locus_tag="K8Z20_34150"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421374.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_021727498.1"
                     /translation="MELLEAILSNKNMNEAYLRVYKNKGVSGVDGVTVEELKQYLKEHK
                     DELRQRIRTRKYQPQAALRVEIPKENGKMRKLGIPTVVDRVVQQAIHQILSPIFEKQFS
                     EFSYGFRPKRSCEMAIVKSLEFLNNGYDWIVDIDLERFFDTVHHDKLMRIISNSIHDGD
                     VISLIRKYLVSGVMVNGKYEETPVGTPQGGNLSPLLSNIMLNELDKELEGRGLQFVRYA
                     DDALIFVRSEKAAGRVMKSIVRFIEKKLGLIVNTEKSKISRPKDLKFLGFGYYYTLKDK
                     RYQTKPHPISVQKFQRKLHQLTKRSWSVPLDYRILKLKQVIFGWVNYFRSTNMKGVLER
                     VDQKLRARIRVIIWKQWKVPKKQIKSLVQLGIPEEEAKGLTYCRKGFRYIGLSKVVQRA
                     ISNQRLKKRGFPSSLERYLKVHTVI"
                     /name="ltrA"
     gene            6864..7448
                     /locus_tag="K8Z20_RS34550"
                     /old_locus_tag="K8Z20_34155"
     CDS             6864..7448
                     /locus_tag="K8Z20_RS34550"
                     /old_locus_tag="K8Z20_34155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021727497.1"
                     /translation="MKNRYEHLKNGYTAIFFKDSNNKETAFFIDTDCFEIANSHDFYWE
                     NYLVNSQGKRMRYNIIGRKKGESSKTQLSRLLLECNTQKEACYNITGNPLDLRKCNLLK
                     TNMGKVKTKEFQEEMNKLIKTVPPLPQIQLKNINEKEKDVAISPKNDNIRLLTDEQNNT
                     YILLENGIITAEIEKKYAKFLVKQLSHSITT"
                     /name="hypothetical protein"
     gene            complement(7801..8370)
                     /locus_tag="K8Z20_RS34555"
                     /old_locus_tag="K8Z20_34160"
     CDS             complement(7801..8370)
                     /locus_tag="K8Z20_RS34555"
                     /old_locus_tag="K8Z20_34160"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="WP_021727495.1"
                     /translation="MKVFEVLESLKKGEKLKEIANVLGINNSTLQRKLKALGYAFDSST
                     KEWFYNDKDNPEPLEENIMDMVKRNSKSGVNDLPKIIQGIKSETKGELGVKHGNTVKVS
                     EVLTSEDIADLHEMLLFWRTDRERTKKHKTLHEEIKTLRRDDRTRKTVILSKEVAKRLD
                     KLSERERFDKSDLLELALEHFLSEYE"
                     /name="ribbon-helix-helix domain-containing protein"
     gene            complement(9692..9919)
                     /locus_tag="K8Z20_RS34560"
                     /old_locus_tag="K8Z20_34165"
     CDS             complement(9692..9919)
                     /locus_tag="K8Z20_RS34560"
                     /old_locus_tag="K8Z20_34165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869766.1"
                     /translation="MKVVNNFRVIMAVKNIDNVAELVRMTGVSRNSINKLWRNDNVSSL
                     RIDTLLPICEKLNVKLSDLIEYIPDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            10278..10565
                     /locus_tag="K8Z20_RS34565"
                     /old_locus_tag="K8Z20_34170"
     CDS             10278..10565
                     /locus_tag="K8Z20_RS34565"
                     /old_locus_tag="K8Z20_34170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000332102.1"
                     /translation="MAYVISKKVKGNIYFYVAQYVGTQPYYSKQYKYKCIYAIGNQKIA
                     LERIAMWLLDNNRIPKELLEIGVSINDVKYWYEKVEKTLQNYSLTNHKNT"
                     /name="hypothetical protein"
     Typing          complement(1216..2376)
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.525"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cctgcaaaca aagtgaacgt atttattcga ttggaaacga aagaatcaca ttagaaaggc
       61 tcactttatg gattttagat aacagtttca ttccaagcga attaataaag attggaatat
      121 caattgacga tatagaaaac tggagagaaa aagtagaaaa taccataaaa agatactctc
      181 tataactaaa aacaagaaaa aaagactgat atttttgtca gtcttttttc tttaactatt
      241 tatatttttt catcacaaaa gggttaggtg ttttaataat atatttatat atttaataaa
      301 agttatgcgt gactttttga ttttttcatt tgcgaaccaa agcaaacaga ctttaagagt
      361 aaaaaggatt aaaaatatac aggaatcacg aaataaaaaa ttcacggaaa taacaaattc
      421 aaatataatc caaacatctt tttttcatca cacgatctat aataactcat ttaaaacggt
      481 gcaacttttt aaagatacaa agtatctata gattgaatat gcgagcaaag ttttttcgtt
      541 ttcagttcgt catggaggta ctatataaac tccgtaattt tggagtacag aattacagaa
      601 aaaacggaaa acaaaaattt cgaaaatcga aaaatcgaga tttcaaattt aataaatttc
      661 aacatcaaat ctcatatcaa aagtccaaaa tcaacctcaa aaaaaattta gaatttccca
      721 aaacttattt tcaaaaaaat attaaaaaac gtgcggtatt ttatcgatat aaatatatct
      781 aaaaaaattc atgcgcacac cttaggtttt cacctcgtac agtagtaaaa cgaaatcatc
      841 agaacaaaat atatctcgaa ataaacttta aaaataaact cacaaattca cgaaatttaa
      901 atttaagaaa aaatctttct tgttagttct cagttagatc ataaaaaaac ttttgaatgc
      961 tacttgctac actaagaagg ttttctttca atacaacaaa gagagaacca aacttaatat
     1021 taagcctggt tctctctttg tcattcaaac atcatcaacc gcaccacatt tttcacattt
     1081 aacccaacat ttatatgagt aatatacatc ttcaccatct ttgaccttac tttttgaaat
     1141 ccgataaatt ttcatttctt cgttataaca ttcccaacag gtatgtttaa caatatcacc
     1201 ttttttatac aaaactattc aacctccatt tcatcaatca aatgaccgtt atattcttgt
     1261 ttctcacttt ccgttaactc ttgatattct tgttcaaact tcatcaaatc cttattccac
     1321 attgcaatta atctataata atactcattt ttatctatac tcttatactt gtccaattcg
     1381 ccattttcgt attttttcgc ataatctttt aacaatccag cgtacacaat taattgtcga
     1441 ccttttaaag ctaaataaaa cacgtcaaac acatcttgac tcactgacat ttcataatct
     1501 tttgctgaat acttcgcaac ctccgcaact gcatttcctt ctctgattaa ctctactttt
     1561 tgaatatgta cttgcgtaat ctctggcata tctgttactt ctcgccacat ttctaaccat
     1621 tcttcctgtt taatataaag ttcttttttc ttaaaatagc ttttatcaac acaaagtaat
     1681 acgtgaaaat gaggattata tgtatcataa ttcgggttat gatcatctac tttcaagcca
     1741 cgagaatcat aatatgcttt tctcttttta ttgttataca tttcttcagt gataaatcgt
     1801 tctttatcat acgtcatttc tagctttcta atataaccct taatagagcg ttgaatatta
     1861 cgtctatcaa ataacttttt aaaagcttta ttaaatctat caatctcgcc ttttaccata
     1921 tcagctttaa tatttggggt tgttaatgtt aggaaaagaa actctttttt ttcttcaatc
     1981 ttgattgctt ccatcatcgt tgcgattttc actgcgtcct ttcctgcttt tctccaagcg
     2041 caaattggac aaaaacgatt tttgcaatca ttccccttta ctcgtttctt tttcttttta
     2101 tctttcgtag ccacaaactc attaaaagta ctacatgatt taaacaattc tctcccgctc
     2161 tcactcattt tcttctctat aaattcataa attcttatat tctgtttctt tttaggttgt
     2221 aatttaccag tgatttcatc ttgatttttc tttgtcatgt ttgatatatt agtcctatct
     2281 gaattgctta aaaaagggta tacgaatccc tttttcggac ttgctccttt cttggtagtt
     2341 attgttttta attcgacact aaaaatgata aatctatcca cctaggaatg caagtctttt
     2401 tttttagaaa tcccttgtgt atcaagggat aaaaccatac ataggcatca atttcgttgc
     2461 cgaaatagtt acctatgtat taaagaaaga agaaaaccgt atttttcgtg tccgaaaaat
     2521 cataaaaaaa tgcccctcaa tccacttcgt ggacaagggc atttttttct ttcaaacaca
     2581 aaaattcttc tatctcccaa acgcttcatt ttcacttcta aacgttttta agagatttat
     2641 aatattttcc ccttaaggaa tacatgatga tagcgtaaca aagaaataaa ctcgtcaatt
     2701 agtatccttt ctttcatggt tttctccata aattttaaag tgtatttttt tctcattttt
     2761 ttaaaacact ttttttacac aaataaaaga cctgaaaacc cttgatgttc ctgtattttc
     2821 gatagattta acccctttat ttttggggtc acacaacccg tattacatag gggggttgtg
     2881 acgtcatcat tcatagcaaa ttcttaaccc caaaacagat atttttgtgt gcttgtccga
     2941 gatgcttttt aaagaagtgt gagaaattct caacctttac ctgcttatta gagtaacagg
     3001 taactttctc tgcacctcta ctctttttta caaacaaaac gtatcaagca cattgtcttt
     3061 ttcctctttg ttgataccaa tgtatttgag cgttatttta ggtgcgctat gattcaaaat
     3121 ttcttgcaac atagcaacat cttttgtttg tttatagaac caatagccga aagtctttct
     3181 catggtatga gtaccgatag attccactcc tgcgaaatct cctgcctttt gcaactgtcg
     3241 gtacacttgg attttactta taggcctgtc tcctttgcga gaaggaaaaa gccatttact
     3301 ttccaatcct gctgcataca aataaacctc ttcaaaaata gatgtaatgt tcaccattcg
     3361 ttcctttttc gttttgcctt ccctaatttt caattctttt ctacgtttct tcttcaattc
     3421 caaaatcata gatgtttcta aattcaataa gtcactcaca cgaagacccg tattgattcc
     3481 aatcacgaat aaaatataat cacgatcagt gcaatgacgt ttgagcgccc atttcatatc
     3541 ttgcaactgc tccttgcttc gaatcggttg tacatcaatc aaatgctctt ttttactcat
     3601 cagcccatac cctctttatt tacagtgttt tgaatgtata ctttttgttg tatatgttga
     3661 gagtatcagc aattttgatg taaagtcaat gatttcgtat ttttgaatgt aaactaatgt
     3721 gtaaaagttt acattcaaca tttaacgcaa aagagacatg tacaaataca actaacgtcc
     3781 actagcgagg gcatagcgta tcgctatgcc cttccaaacg aattcacctt gtcttccccc
     3841 tcttttgtca gcgacaagta gcgaggacgt tatacaaggc tctacaatct tccctatctt
     3901 ttgcaccgaa atataaattt gtttacatat ttctaaaaat gagtataatt gtattaacgg
     3961 ttaatacgtt ctaactgtta taactttcta actaaacgtc ataataatac agagagatta
     4021 aacacaacaa acaaaagaga aaactaaaaa gaccgaaaaa acccaaagca aacaaacaaa
     4081 acaaacataa aatcacacta tataattcga aaaactcaat acaaacacaa agtggcaatt
     4141 agactacgcc aaaaaatagt gtggcaaatt ccaaggagga agtccaaatg acagcaaaca
     4201 ctcgccacat ggaaacacaa aaaatcgaac gtaaagttac taaaatcggc aattctttag
     4261 gtttaacatt accgcaagaa ttttacacta ctctaaatat caaacaaggt gacgttgtaa
     4321 gtttagaaat taacgaagaa aacgaacaaa tcgttataaa aaaatccaaa aaaatttcta
     4381 tgccagaggg tctcaatcct aagttcttgg aatccttaaa cagaaccatc aataaatttg
     4441 acggtgcatt taaagatttg gtaaacagat aattatcctt caggcgtgga ggaaagtagg
     4501 aaaaccgaaa tgacaattta tataacagaa actcaagcta tttttatcaa tgaaacactt
     4561 atcgatatgt attcacctaa cgaacaaaga ggtgttaaag atacggggtt attacaatca
     4621 gcaatatacc gtccacaaca aacagtagca caagaggatg catatccgac tatcttccac
     4681 aaagccactg cattatttga atcactggct aaaaaccatg cattctataa tgcaaacaaa
     4741 agaacggcat tggcttgcct agaaatgttt ctgttataca atgagtacga attaaaaata
     4801 tctgaacaag aagcttcaga cttcactgtt aacgtggtag aacagcgatt atcttttgac
     4861 gaaattttaa atgtcataaa agaaaactca tcaaaattac cttcaaatta atcgagtaga
     4921 gggaaaataa agtaattatt ctcgcccctc tcacaccacc gtacatacgg ttccgtatac
     4981 ggcggttcag tttatattac agtgtgaact tttagatagc gttctaaaga agaagggaat
     5041 cccctcttct ttagtctttg atttgagatg gctctttgaa caactttcga taaccctata
     5101 tatcggaagc cctttcgaca gtaggttaat cccttcgctt cttcctccgg aatccctagt
     5161 tgaacaagcg atttgatttg tttcttcggt actttccatt gcttccagat aatcactcta
     5221 attctggcgc ggagcttctg gtctactcgc tccagaacac ctttcatatt tgtactcctg
     5281 aagtaattga cccatccaaa tatcacttgt ttaagtttca atattcgata gtctaacggt
     5341 acactccagc ttcgttttgt cagttggtgc agttttcttt gaaatttttg taccgagatt
     5401 ggatgtggtt tcgtttgata tctcttatcc ttgagggtgt aataatatcc aaaccctaaa
     5461 aattttaaat cttttggccg agagatttta ctcttttctg tattgactat caagcctagt
     5521 ttcttttcta tgaatctcac gattgatttc atcactctgc ccgctgcctt ctcactcctc
     5581 acaaagataa gagcgtcatc agcgtatctc acaaattgaa gtcctctacc ctcgagttcc
     5641 ttatccagtt cgttcaacat aatgttactc agtaaagggc tgagattgcc tccttgcgga
     5701 gttccgactg gtgtttcttc atatttccca ttcaccataa ctccactgac tagatacttt
     5761 cttatgagag aaatgacgtc tccgtcatgt attgagttag atataattcg catcaattta
     5821 tcgtgatgaa ctgtgtcaaa gaatctctcc aaatcaatat ctactatcca atcatatccg
     5881 ttgtttagaa attccaagct tttaacaatt gccatctcac aacttctttt tggtctaaaa
     5941 ccataactga attcactgaa ctgcttctca aatatcgggc tgagtatttg atgaatggct
     6001 tgttgaacga ctctatccac tactgttggt attcccagct tgcgcatctt gccattctct
     6061 tttgggattt ccactcttaa ggcagcttgt ggttggtatt tccttgttct aatgcgctga
     6121 cgtagctcgt ccttgtgctc tttcagatat tgctttagtt cttcgactgt tacaccatca
     6181 actccactaa cacctttgtt tttataaacg cgtaagtagg cttcattcat atttttgttg
     6241 cttaaaattg cttctaagag ttccacactc gtttctcctt tcccttcacg tagtaagtga
     6301 caactccatt ttgattatcc tttgagatac gctcatactt tcatcattaa catattcgga
     6361 gtacgtcact tacgcattca tggcgttgaa acactataaa ctgttcggcc cttcatgaat
     6421 ttgttccact actatggctt cggctgactt cttgcggtta accattttcg actgtactgt
     6481 tagtacatcc gcaagatctc ccagggtaag gcagctatct ttcctctttt actcgcctga
     6541 tttactctac aaagttacgc acatcttttg gactttaact tgttatggag cctcatccct
     6601 ttataaagcc ttcatatcag gtttctgttc gtcgagccaa gattttattc cacgcttcct
     6661 ccagccctta cctcacggta agtaccttgc gcttccttag tggttggtcg atgtgtaccc
     6721 ccacagtgga cttccaccac ctagatagtt gccatgcctg gcacacataa aaaaagacgt
     6781 ggtcaaatac cacgtctttt tttattacaa ccttttattg tagttctgaa tattctataa
     6841 tctttaacga gaggaggaaa ttcatgaaaa atagatatga acacttaaaa aatggctata
     6901 cggcaatttt tttcaaagac tccaataata aagaaactgc tttctttatt gatacagatt
     6961 gcttcgaaat agctaattct cacgattttt actgggaaaa ctacttagtt aatagccaag
     7021 gaaaacgaat gcggtataat atcatcggaa gaaaaaaagg tgaaagttca aaaacacaac
     7081 tatctcgatt actcctagaa tgcaacacac aaaaagaagc atgttacaac atcacaggaa
     7141 accctctaga tctacgaaaa tgtaatttac ttaaaacaaa catgggtaaa gttaaaacaa
     7201 aagaatttca agaagaaatg aacaaactta tcaaaactgt acccccatta ccgcaaatac
     7261 aattaaaaaa tatcaatgaa aaagaaaaag atgttgccat ttccccaaaa aatgataaca
     7321 tcagattact aactgacgaa caaaacaaca cttacatact tttagaaaac ggtataataa
     7381 cagccgaaat cgaaaaaaag tatgcaaaat ttctcgtcaa acaacttagt cattctatta
     7441 cgacttaaaa atctataagt aactttgaat acattctaaa agacacaatt tatatgtcaa
     7501 taaccacaaa aaaagaatcc tatttacagg gttctttttt tattactttc tatccttctg
     7561 ctaataatcc attcaattct tctgtggcat ccttaggatg ccaatagcca ttctccaaaa
     7621 gttttagata atatccccat atactcacat caacaatttg cgaggacatt atgcaaggct
     7681 ctacaaacct cgtaagatca ataacaataa tttgcgagga cgttatacaa agctctacaa
     7741 tcttccctaa aaaaaataaa aaggtccata atggacctaa taacaaacaa caatctattt
     7801 tcattcatat tcactcaaaa aatgctccaa tgccaattct aataggtcgg acttgtcaaa
     7861 acgctctctt tctgacagtt tatcgagacg tttcgcaact tctttgctca aaataaccgt
     7921 ttttcgcgtc ctatcgtccc ttctaagagt ttttatttct tcatgaagag ttttgtgctt
     7981 tttcgttctt tctctatctg tacgccaaaa taagagcatt tcatgaaggt ctgctatatc
     8041 ttcacttgtc aaaacttcag atactttcac tgtattacca tgtttcactc ctaattcacc
     8101 tttagtctcg cttttaatac cttgtattat cttaggtaaa tcattaacac cacttttact
     8161 atttcgcttc accatatcca taatattttc ttctaatggc tctgggttat ctttgtcatt
     8221 gtagaaccat tctttagtgc tactatcaaa agcatacccc aaagccttca gctttctttg
     8281 taacgtgctg ttgttaattc ctaaaacatt agctatctct ttcaatttct cgcctttctt
     8341 gaggctttct aacacttcaa acaccttcat attgctttca tccccttcac atatatccca
     8401 cacaaataaa attcaatgtg taatatgcaa taccttcata caacattaaa gtgaaactaa
     8461 tattactttg tatgactata tgtgaattag agtcacacaa tagcatggag aacaaatatt
     8521 cttaatacaa ttatgttccg ctgcgcttta cgtctaaagg gactataagt gcatccaaac
     8581 aacactcctt aactgaagct ttgcccaacc ccatataaaa atatcaatcc cccaacccct
     8641 tctccaaggg gattccctcg gcggtagcca gcccaaaacg tactgcgttt tgaggactgc
     8701 aagagggtct tggtcaaagt tccctatcaa ctccttaatg ctccatcatt tcgctatcgc
     8761 ttcattccgt ccgcttaccg ttgacaggta ctgtatgtat tcctccaccg ttcaaaagct
     8821 aacatccata tattaaaaag tctcttttta tattcaccat gacaacataa tgtctccgct
     8881 gccttgcaaa atatcctcgc caacactcgc catttttttc acacctccca cgatccaggg
     8941 gaattttttt gtagtggaat gactacttcc tctacaaaca taattatttt tttgttttac
     9001 aggaagagcc tcaactattc tctacttttc aaacatcatc caatctattg atacaatcac
     9061 attctttatg agaatcactg tcggaacagc catagaatag aactacagct atttctatat
     9121 tcattcctag acccattcca ataaaaaaga gcataaggaa atccgatccc acttctagtt
     9181 caaagtggtg aaataggctg tgcctactga atttctaaag ctctctaact gggattcaaa
     9241 aaacaccctt gtaggtcgtt ttgagtcctt ttaacatcta tttgtaggtc tctttgagac
     9301 ctttgtagta cgttttgtgg acgttttaaa agtaattagt aacaaataaa aaaagagccc
     9361 cataaatagg gactcttttt tcaatactca tcaattccac catttcactt tactgcatta
     9421 cgatgattgc acataagatt caacaaaatc acgaagtatc tgcctacctc tcatcttcca
     9481 agatgcgtca acacctagca agcctataat ttcattgacc gtattcatgt gataacaaac
     9541 agaaaaagac agtcataaaa gtttgccgaa atttaaaata cgcatataac cctaccaaaa
     9601 accatatgcg aatataacct gttttccact agcaaatatt aataccgatt tcatcataca
     9661 caagctatct aaagatagaa tagcccctaa attattttgt ctcactatca tctggaatat
     9721 attcaattaa atccgatagt ttaacattta acttttcaca gatcggtaac aatgtatcta
     9781 ttcttaaaga cgacacatta tcattacgcc acaacttatt gatagagtta cgacttaccc
     9841 ctgtcatcct aaccaactct gcaacattat ctatattctt taccgccata atcacacgga
     9901 agttatttac tactttcatt ttttatactc ctttttctca tgaaatatac atactatttt
     9961 tcttataaag gcacttttgt gcaaataata gttgtttttt aaccttttaa acacaacttt
    10021 agcactttat aaaacatttt taaacctgta tggaataaaa aatcacatta tgcattattt
    10081 catacttcat tcaaacgaaa attaatttct atacatataa ggttatttat tgcgttcatt
    10141 taaactaatt taaaacctat ttaaataaaa atgttcaaaa tatgcattat ttcatacttt
    10201 atttgttgaa aaattaacct aataaagtta atattaatca atgtgatata gaattttaat
    10261 aattggaggt cattattttg gcgtatgtaa tatcaaaaaa agtaaaagga aacatttact
    10321 tttatgtagc tcaatacgtt ggaacacaac catattactc aaagcaatac aaatataaat
    10381 gcatttacgc aatcggcaat caaaaaatag ctttagaaag aatcgcaatg tggctattag
    10441 ataacaaccg tataccaaaa gaactactag aaatcggagt atccataaac gatgtgaaat
    10501 attggtatga aaaagttgaa aaaacattac agaactactc tttaactaac cataaaaata
    10561 cctgaaagga gcattaacat ggcatttcta acaaattaca aagcaaacgg aaaaagatac
    10621 ttttatgtag aaaaatatgt tggtaaaaaa ccatata
//
