LOCUS       NZ_CP083164            12727 bp    DNA     circular CON 11-APR-2024
DEFINITION  Bacillus thuringiensis strain ABTS-1857 plasmid pCH_181-h, complete
            sequence.
ACCESSION   NZ_CP083164
VERSION     NZ_CP083164.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214332
            Assembly: GCF_020809245.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 12727)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12727)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x, 40x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809245.1-RS_2024_04_10
            Annotation Date                   :: 04/10/2024 23:39:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,957
            CDSs (total)                      :: 6,806
            Genes (coding)                    :: 6,416
            CDSs (with protein)               :: 6,416
            Genes (RNA)                       :: 151
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 175 of 390
            Pseudo Genes (incomplete)         :: 226 of 390
            Pseudo Genes (internal stop)      :: 99 of 390
            Pseudo Genes (multiple problems)  :: 87 of 390
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083164.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12727
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="ABTS-1857"
                     /isolation_source="Biopesticide XenTari"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_181-h"
                     /collection_date="2017"
     gene            join(12343..12727,1..41)
                     /locus_tag="K8Z20_RS34420"
                     /old_locus_tag="K8Z20_34025"
     CDS             join(12343..12727,1..41)
                     /locus_tag="K8Z20_RS34420"
                     /old_locus_tag="K8Z20_34025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015484803.1"
                     /translation="MMEKFVKFLKGGFEAVIKSLKTFIDFLATPFQYLLDFLKGIFYFI
                     EKLFQVVVAIIKLFVALFQFLGAVIAGFFKTLFLWVQPNFDGGNVSFPSASMKGFKTAT
                     DILLPTGLLTVVPMVVTCFIWYLFVKKLMDLFRGGHH"
                     /name="hypothetical protein"
     gene            41..301
                     /locus_tag="K8Z20_RS34425"
                     /old_locus_tag="K8Z20_34030"
     CDS             41..301
                     /locus_tag="K8Z20_RS34425"
                     /old_locus_tag="K8Z20_34030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000992627.1"
                     /translation="MNALIDTIFNPVLGWLAQMAKYLNDLSVPASRPLRISDYLGVFAY
                     LYGWTTVITTLFALVFVYFVCYIIMTYSSLFIRFKDTVKWW"
                     /name="hypothetical protein"
     gene            608..970
                     /locus_tag="K8Z20_RS34430"
                     /old_locus_tag="K8Z20_34035"
     CDS             608..970
                     /locus_tag="K8Z20_RS34430"
                     /old_locus_tag="K8Z20_34035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000880239.1"
                     /translation="MLAKTKRKVQPQFEGHRALIIKEEGTCTVEQVISFDSEILETSTA
                     MLPRDNELRLLDDNHGYVYHIYNLEKPARIEAENIKRLRRSSALKGIFEFETGKKQFDL
                     IGFMPWVIIVLLAIFK"
                     /name="hypothetical protein"
     gene            984..1391
                     /locus_tag="K8Z20_RS34435"
                     /old_locus_tag="K8Z20_34040"
     CDS             984..1391
                     /locus_tag="K8Z20_RS34435"
                     /old_locus_tag="K8Z20_34040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015484804.1"
                     /translation="MSEVTLEKTVVEEVLPDVQDIMKEEILEEDTSSESIETVQLFQYM
                     KENSVPLNASQMKGILLLNENGCKDIADYVISVKKQSLPLAFYEKVLKILTMFDRIKGN
                     AKLSHLLKANANPSLSLSANSAKVVKGNELA"
                     /name="hypothetical protein"
     gene            1388..1990
                     /locus_tag="K8Z20_RS34440"
                     /old_locus_tag="K8Z20_34045"
     CDS             1388..1990
                     /locus_tag="K8Z20_RS34440"
                     /old_locus_tag="K8Z20_34045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015484805.1"
                     /translation="MNVMVFEGPLGSGKTLGMTLFAHHFKQKSNCVLYSNYGVVGSKPF
                     TEIEHFKNIAQEKSTILNLDEAHIDLDARSFSSNSVKFFSQVSYYLRKLRCTLFIASPS
                     FDDLDSRIRGITNVLVKVSSDKKYFYYTMYDIQSKRYLKRMRISKKKAFVVGSKIYDTS
                     AMVSPVKVPEKRQDFMEFLEALKTTAEEYGRQYKHSA"
                     /name="hypothetical protein"
     gene            2061..2252
                     /locus_tag="K8Z20_RS34445"
                     /old_locus_tag="K8Z20_34050"
     CDS             2061..2252
                     /locus_tag="K8Z20_RS34445"
                     /old_locus_tag="K8Z20_34050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015484796.1"
                     /translation="MGILKKKRFREEVKRINKAHEEMKRFLDVLMDRYGLDEEEINNCE
                     VIKHHFDNLDLMFSQMAK"
                     /name="hypothetical protein"
     gene            2884..3090
                     /locus_tag="K8Z20_RS34450"
                     /old_locus_tag="K8Z20_34055"
     CDS             2884..3090
                     /locus_tag="K8Z20_RS34450"
                     /old_locus_tag="K8Z20_34055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015484797.1"
                     /translation="MDLPICNEKEVTVLSKTLKYLRSDQSNQVKVHKEYLEDRGWIFDD
                     LSYIDSHIYVVYKKEVVKRNKAF"
                     /name="hypothetical protein"
     gene            3102..3521
                     /locus_tag="K8Z20_RS34455"
                     /old_locus_tag="K8Z20_34060"
     CDS             3102..3521
                     /locus_tag="K8Z20_RS34455"
                     /old_locus_tag="K8Z20_34060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015484798.1"
                     /translation="MFKKFYREYIHVLFNIVAMFFYFSWCLVGTEFNLLTLIAIGIAIF
                     IFVKYVSMDRKFVNENIDKLKDKKMIFRKYAISGENIGYAVFTNFLLFLLYLGISVYFK
                     DLSVISLLFAFILNVSLLMLTLRVMSKFSLKNSKV"
                     /name="hypothetical protein"
     gene            3533..3868
                     /locus_tag="K8Z20_RS34460"
                     /old_locus_tag="K8Z20_34065"
     CDS             3533..3868
                     /locus_tag="K8Z20_RS34460"
                     /old_locus_tag="K8Z20_34065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015484799.1"
                     /translation="MFARYRSQDDEFMSQLQFLALGAIMTFLIKFGCISPEVNMLTLFV
                     LFVILIGIYYLFVLLLTKYFYRFLSKQHDKLHAYLCNKYGEKIANRVGLSFLIILIVGM
                     LIVCLLI"
                     /name="hypothetical protein"
     gene            4189..4404
                     /locus_tag="K8Z20_RS34465"
                     /old_locus_tag="K8Z20_34070"
     CDS             4189..4404
                     /locus_tag="K8Z20_RS34465"
                     /old_locus_tag="K8Z20_34070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001169628.1"
                     /translation="MQKISEERKHRSFRFTDSELSLIDELVDIENDRRMTIAREMNLKY
                     KALNRTSFILYMAEERRRKYEEQGEI"
                     /name="hypothetical protein"
     gene            4575..4853
                     /locus_tag="K8Z20_RS34470"
                     /old_locus_tag="K8Z20_34075"
     CDS             4575..4853
                     /locus_tag="K8Z20_RS34470"
                     /old_locus_tag="K8Z20_34075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021729343.1"
                     /translation="MAYSYCVDCESVYSCEDEKCCCGSKKRSVDWEEFAFIMGYPLYPV
                     KKKYGVFHLDLSKRLDADALCDLKADNPELFYYEFKGWKERELKKRN"
                     /name="hypothetical protein"
     gene            4916..6115
                     /locus_tag="K8Z20_RS34475"
                     /old_locus_tag="K8Z20_34080"
     CDS             4916..6115
                     /locus_tag="K8Z20_RS34475"
                     /old_locus_tag="K8Z20_34080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071621.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_228231856.1"
                     /translation="MRNEEPPTGNTGVPGPFDVYTIPSLDWVQVTFKSVQNSQKNARKI
                     ISKISSFFGVDEFLFEHFDEGLHGYKKSYKFLGQNTFQLLFDAPSSMGIHLILTGSMLK
                     MLRSDYGKGDIQLLKFLSSISKEFHFSRVDVAKDDTSGSVSIKKIARYIKDGNLTTRFR
                     GGHQIKKFKLVGVDDEEDKLQYVPDGETWYLGSRSGTQFRFYDKKAQMNADDLFHWTRC
                     ELQLVDDAATNFVKNVINLDKHNFEKFCYSVFLTYVDFKKTTGATRMKRRDTAKFWADF
                     LDSTVEKVKLGSRKKKKSLDDINPEYLWQKLLDGDADFYYESMHQKEKDEFDKWFFEQI
                     SSVLYLRALKNEEGIEEFYKKLIHWGETRVDQKKVEAVGVQLLMSERLKRIKEKESPID
                     "
                     /name="replication initiation factor domain-containing
                     protein"
     gene            6806..7846
                     /locus_tag="K8Z20_RS34480"
                     /old_locus_tag="K8Z20_34085"
     CDS             6806..7846
                     /locus_tag="K8Z20_RS34480"
                     /old_locus_tag="K8Z20_34085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085408.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_042991732.1"
                     /translation="MAKKNQDEITGKLQPKKKQNIKIYEFIERKMSESGRELFKSCSTF
                     NEFVATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADMVKSEIDRFNKAFNKLFKRRNIQRSIKGYIRKLEMTYDKERFITKGMYKD
                     RKAYYDKRGLKVDDHNPNYDTYNPHFHVLLCVDKNYFKRKELYIKQEEWLEMWREVTDM
                     PEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQDVFDVFYLALKGRQLIVYGGLL
                     KDYAKKYEDGELDKYKSKDKNEYYYRLIAIWNKDLMKFEQEYQELTESEKQEYNGHLID
                     EVDVEA"
                     /name="protein rep"
     gene            7996..8151
                     /locus_tag="K8Z20_RS34485"
                     /old_locus_tag="K8Z20_34090"
     CDS             7996..8151
                     /locus_tag="K8Z20_RS34485"
                     /old_locus_tag="K8Z20_34090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021729289.1"
                     /translation="MGRKRIDPDEKKTRLNVAIKKKYVDLVNQEENTSQFVEDAIVKYL
                     KENKKI"
                     /name="hypothetical protein"
     gene            8295..8558
                     /locus_tag="K8Z20_RS34490"
                     /old_locus_tag="K8Z20_34095"
     CDS             8295..8558
                     /locus_tag="K8Z20_RS34490"
                     /old_locus_tag="K8Z20_34095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021729290.1"
                     /translation="MPQAIIEGQYLSSSIKKSTFNGVEKSFVQLDVYQPESTDSEKTVV
                     VKCDDLELINHFKDTKMGMPIKAKVSINAYQNKAYFKLVNLA"
                     /name="hypothetical protein"
     gene            9195..9347
                     /locus_tag="K8Z20_RS34495"
                     /old_locus_tag="K8Z20_34100"
     CDS             9195..9347
                     /locus_tag="K8Z20_RS34495"
                     /old_locus_tag="K8Z20_34100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001113828.1"
                     /translation="MPEFGFRPSMSQLFGNFHMNWSTMMPLLVILFGILFGFFVAHLVK
                     DKFDD"
                     /name="hypothetical protein"
     gene            9368..9526
                     /locus_tag="K8Z20_RS34500"
                     /old_locus_tag="K8Z20_34105"
     CDS             9368..9526
                     /locus_tag="K8Z20_RS34500"
                     /old_locus_tag="K8Z20_34105"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425805.1"
                     /translation="MEMPKISLTYNLADVANGLTNWLGAYWPIIAFSVAIPLTFLVAFN
                     TKDLFTR"
                     /name="hypothetical protein"
     gene            9633..11015
                     /locus_tag="K8Z20_RS34505"
                     /old_locus_tag="K8Z20_34110"
     CDS             9633..11015
                     /locus_tag="K8Z20_RS34505"
                     /old_locus_tag="K8Z20_34110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_161513874.1"
                     /translation="MKKAAIIFLCTFCIIFRTVPVFADTPSFQMVGSKGVSIENKGLVG
                     GYYSLSLKISDAKDVVTFQLSELMLVNDCSFNFGLVKWTRPSQEKNEFDIKVKENGTYA
                     FYVFVNGKVKGYVRFKIKGFPKNAEGAGVTQYWGQGAKYYELPEEHVNPEDFRDKDGEN
                     NGICVNEKISDDKPGGSGDIEEPKEDNKPPVEGGGGSDKAIKEALDKITSAVNEISKNT
                     GETAKNTGEIKKNTDEIAKNTKEIADAVKDIRDNLKIPDDMDLSIKPVLPEDIKPDKPK
                     QPDKPFEDKQEHFKEGEQEKPPDKLPVAPEPAECWGDVCKDKDNQKEEDMKKDKDMDKE
                     EPMKPDKPMEKEDMKPDKPMTPDKPMEKEDMKPDKPMTPDKPMEKEDMKPDKPMTPDKP
                     MEKEDMKPDKPMTPDKPMDREGEMKKDNDMKQDKPMTPWEEMKPSDEGSELCWHVVNGK
                     CG"
                     /name="hypothetical protein"
     gene            10985..12181
                     /locus_tag="K8Z20_RS34510"
                     /old_locus_tag="K8Z20_34115"
     CDS             10985..12181
                     /locus_tag="K8Z20_RS34510"
                     /old_locus_tag="K8Z20_34115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090439.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="WP_155278152.1"
                     /translation="MACSKWKMRIKKFLLVVPLLLGILFIGDTSAFAEVSRASLGNDLL
                     KGVSGSMCGSTVTKITDGNPSTGETLFKTCREIRFKLPKVSNVSGFLYIKTNKWTSDKL
                     AIDFKKVDGQVKTITYNQASVNSNNYIQVFVKDVSEVVLRNFDDTGAIEIMSFELYEGN
                     GDEFFYEDIKELQPTITYDSVTFSYKNPKIDFGYLRIYRDGKLIVDNFKLEQFKDKELS
                     PNTEYKYKFVAVSSDGIESKGIEKVIKTDEKPNLAKPIKPSNPTITPKDGSLIVNLTNY
                     DAGVKVKGYHIVVDGKQVNDSLVTARSYAITGLKNGQSYQVQIKAVSAWNIESDLSNSV
                     PGIPQVQVIPDIAFNFGLSDLMESIKNWFGGIWPIVAFSIAIPLTFIVAFNTKKLFLR"
                     /name="fibronectin type III domain-containing protein"
     Typing          5091..6102
                     /name="rep_cluster_485"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.09"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          6672..7828
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.917"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4881..6112
                     /name="MOBT"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.241"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaaaaagct catggatcta ttcagaggtg gtcatcatta atgaatgcgt taattgatac
       61 aatatttaat cctgttcttg gttggcttgc gcaaatggca aagtatttaa atgatttaag
      121 cgtaccagca agtcgtcctt tgcgaattag tgattacctt ggagtgtttg cttatctata
      181 cggttggaca accgttataa cgacgttatt tgctttagtt ttcgtatatt ttgtttgcta
      241 tatcatcatg acttacagta gtttatttat tcgatttaaa gatacggtta aatggtggtg
      301 acgatattgg atattatagg acaagctatt ttattaatat tttttacgac aattgtttct
      361 tggatggtaa tagatacatt gaaattcttt agaggtatag aagaatgatt tatttgatat
      421 tgggagtcgt tagttgttta ttaattttag tttgtgatgc aacttcgaaa aaggagagtt
      481 aaaaatgcag gcttttaatg caatgtttga gttatcaagt gcgatttgtt tatctgtatt
      541 tctatttata gtgttacctt gtatgtggtt aaaaagaaaa tggaattaaa aagagagagt
      601 gattgaaatg ttagctaaaa cgaaacgaaa agtacagcca caatttgaag gacatagagc
      661 gttaattatt aaggaagagg gtacatgcac tgtagaacag gttatatcgt ttgattcgga
      721 gattttagaa acgagtacag ccatgttacc acgtgataat gaattgagat tacttgatga
      781 taatcacggt tatgtgtatc acatttataa tttagaaaaa ccggcaagaa ttgaagcgga
      841 aaatattaaa agattacgac gatctagtgc gttaaaaggg atttttgaat ttgaaacagg
      901 aaaaaaacaa tttgatttaa ttggttttat gccatgggta attattgttt tactagctat
      961 ttttaaataa tggggtgaac tgaatgagtg aagtgacttt agaaaaaaca gttgtagaag
     1021 aagtgttacc agatgttcaa gacataatga aagaagaaat tttagaagaa gatacttcaa
     1081 gtgaaagtat tgaaacggtt caattatttc aatacatgaa ggaaaattca gtgcctttga
     1141 atgcatctca gatgaaaggt attttattgc ttaatgagaa tggatgtaaa gatatagctg
     1201 attatgttat ttctgtaaaa aaacagagtt taccacttgc cttctatgaa aaggttctaa
     1261 aaatattaac tatgtttgac agaattaaag gtaatgcgaa attaagtcat ttactgaaag
     1321 cgaatgcgaa tccatcatta tcactttcag ctaatagtgc aaaagtagtg aaaggaaatg
     1381 aacttgcatg aacgtaatgg tctttgaagg tcctcttgga agcggtaaga cattaggaat
     1441 gacattgttt gcacatcact ttaaacagaa aagtaattgc gtattgtata gcaattatgg
     1501 cgtagttggt tcaaagccat ttactgaaat agaacatttt aaaaatattg ctcaagaaaa
     1561 atctacaatt ttaaatttag atgaagctca tattgatttg gatgctcgaa gtttttcgag
     1621 taactcggtt aaattctttt ctcaagtatc gtattatctt cgaaaattac gttgtacgtt
     1681 gtttattgct tcgcctagct ttgatgattt agattcacgt ataagaggta ttacgaacgt
     1741 tcttgttaag gtttcaagtg ataaaaaata cttttactat acgatgtatg acattcaatc
     1801 taaacgttat ttaaagagaa tgcgtataag taaaaagaaa gcttttgttg ttggttcaaa
     1861 aatttatgat acaagtgcaa tggtttcgcc agtaaaagtt ccagaaaaac gccaagactt
     1921 tatggaattt ttagaagcgt tgaagacgac cgccgaagag tacggacgcc agtacaagca
     1981 ttcagcgtaa gctgtatgcg cggtacaggc gggaggactt cataagatta taaaaattat
     2041 atcaaggaag gaacgttgtt atggggattt taaagaagaa aagatttcgt gaagaagtga
     2101 aaagaataaa taaagcacat gaagagatga aaaggtttct agatgtactc atggatcgct
     2161 atggattaga tgaagaagaa ataaataatt gtgaagtaat taaacatcat tttgacaatt
     2221 tagatttgat gtttagtcaa atggctaagt agatgattga atatattgta ggtgctagtg
     2281 tgctgtggtt aggttcgata gtgattgctt atttattagt tagtgatatg gatatttatt
     2341 agagaggata aatagttgaa atgatacatg tggattggtt gaaaagtatg gcgagagatg
     2401 gactgtttta tggttcattc attgctgtaa ttgttttctt gttaaattgg tttacgggtt
     2461 aggttttaaa ggtattcctt cgcttttgag gttggagatt ggctggggtg tggggctagc
     2521 cccgcaaagc gtaatgggta aatagaataa aagaaaatat aaaggtcggt agtacaagcg
     2581 tttaggctcg tataatggat aggcaagttg atagttaaat atattcactt ttgagtttat
     2641 cgacttggcg atgaagaaaa acgagcctaa aaaagttatg caagaatgta gcgcagcgaa
     2701 aaaggtggag attttatgaa gcagagcgta agaaaatact acctgtttct tgtttaacct
     2761 tttagcaaat acagatagag ggttgttttg atggaaaatg ttattgttac gttgtttggt
     2821 actctaggga tttgtttctt gtttaaaggc atgttagaca cgttaaaaga agggtgatat
     2881 gtaatggatt tgcctatttg taatgaaaaa gaggtaactg ttttatctaa aacgctgaaa
     2941 tatttgagga gtgatcaatc taaccaagtt aaggtacata aggagtactt agaagacaga
     3001 ggttggatat ttgatgattt aagttatata gatagtcata tctatgtagt ttataaaaaa
     3061 gaggttgtga agcggaataa ggcgttttaa gggggatata tatgtttaaa aagttttatc
     3121 gtgaatatat tcatgtattg tttaatattg ttgcgatgtt tttttatttt agttggtgtt
     3181 tagttggtac tgaatttaat ttattaactc taatagcaat tggaattgct atttttatct
     3241 ttgttaagta tgtatctatg gataggaaat ttgttaatga aaatatagat aaattaaaag
     3301 ataaaaagat gatttttagg aagtatgcta taagtggtga aaatattgga tatgcagttt
     3361 ttacaaattt tttattgttt cttttgtatt tggggatatc ggtatatttt aaggatttaa
     3421 gtgtaatatc gcttttgttt gcttttattc ttaatgttag tttgcttatg ttgactctta
     3481 gagtaatgag taaatttagt ttaaagaata gtaaagttta agaggtgtat atatgtttgc
     3541 tagatatcgt agtcaagatg atgagtttat gtcacaactt caatttttgg ctttaggtgc
     3601 tattatgaca tttctaatta aatttggttg tatttcacct gaagttaata tgttaacgtt
     3661 atttgttctt tttgttattt taattggaat ctattatctt tttgtacttt tattaacaaa
     3721 gtatttttat agatttcttt ctaaacaaca tgataagttg catgcttatt tatgtaataa
     3781 gtatggtgaa aaaatagcaa atagagttgg tttgtcattt cttataattt taattgtggg
     3841 gatgttgatt gtatgtttgc tgatttaaag ttgaatttta aattttggtt aattgtagtt
     3901 tttgatatat gtatgcttgc aatgatctgt gttgttacaa caaagtatgt atctgatata
     3961 tatagtgatt tgtttatttt tacgacagga tttttgatat tgttaatttg gtattttgtt
     4021 agagtttatt ttaaaaatat tgttttgaaa tgtattaaaa aagtttaaaa tatgatttaa
     4081 ccatatacct ttgtgtacac ttaaaaatat ttcgtgtata caaaggttgt ttttgtaatt
     4141 taaatgtgtt atattgtgta tacgatagtt ttgtaggagg ttttagttat gcaaaaaata
     4201 agcgaagaaa gaaaacatag gtcatttcgt tttactgata gtgagttgtc attaattgat
     4261 gaattagttg atattgaaaa tgatagacga atgacaattg caagagaaat gaatttaaaa
     4321 tataaagcct taaatcgtac aagttttatt ttatatatgg ctgaagaaag aagacgtaaa
     4381 tatgaagaac aaggggagat ttaaatagtt ctttacttaa cttgttaata ttaatagggg
     4441 ataaacgctc actaagttat atcatttggt tgaggaaaaa gatgttgaga ttgatgtatt
     4501 gaataaacgt ctatttaaga acgaggcgac ggttgaacgt attaagaggg aaaagcatta
     4561 ggaggaaatt gttgatggca tatagttatt gtgtggattg tgagagtgtg tatagctgtg
     4621 aagatgaaaa gtgttgttgt ggaagtaaaa aacgttctgt tgattgggaa gaatttgctt
     4681 ttattatggg ttatccgtta tatcctgtta agaaaaaata tggtgttttt catttagatt
     4741 tatctaaacg tttagatgct gatgctttat gtgatcttaa agcagataat ccagaacttt
     4801 tttattatga gtttaaaggt tggaaagaaa gagaattgaa aaagagaaat taataataaa
     4861 tatattttgg agttagtggg tgttttgttt gtctgattcg aaggaaaatt tccagttgag
     4921 aaatgaggaa cccccgaccg gtaacacggg ggttcccggg ccatttgatg tttacacaat
     4981 cccttcccta gactgggttc aggtcacttt taaatctgta cagaattcac aaaaaaatgc
     5041 acgaaaaatt attagtaaaa tttccagttt tttcggtgtt gatgagttct tgtttgaaca
     5101 ttttgatgaa ggtctacatg ggtataaaaa atcgtataaa tttttaggtc aaaatacatt
     5161 tcagttgttg tttgatgcac cttctagtat ggggattcat ttaattctta ctggatctat
     5221 gttgaaaatg cttcgttctg attatgggaa aggtgatatt caattattaa aatttttgtc
     5281 ttctatttct aaagaatttc atttttctcg tgttgatgtg gctaaagatg atacgagtgg
     5341 tagtgtatca attaaaaaga ttgctagata tattaaagat ggtaatttaa ctactaggtt
     5401 tagaggtggt catcaaatta agaagtttaa gttggttggt gtagatgatg aagaagataa
     5461 attacaatac gttcctgatg gtgaaacgtg gtatttaggt tctcgttcag gtactcagtt
     5521 tcgattttat gataaaaaag ctcaaatgaa tgctgatgat ttatttcatt ggactcgttg
     5581 tgagttacaa cttgttgatg atgcagcgac taattttgta aagaatgtta ttaatttgga
     5641 caagcataat tttgagaagt tttgttattc ggtttttctt acatatgttg attttaaaaa
     5701 aactactggt gctactcgta tgaagagacg tgatacagca aagttttggg ctgatttttt
     5761 agatagtaca gtagaaaaag ttaaattagg ttctcgtaaa aagaaaaagt ctttagatga
     5821 tattaatcct gagtatttat ggcaaaaatt attggatggt gatgctgatt tttattatga
     5881 atcgatgcat caaaaggaaa aagatgagtt tgataagtgg ttttttgaac aaatttcatc
     5941 agttttgtat ttaagagcgt taaaaaatga agaaggtatt gaagagtttt ataaaaaatt
     6001 gattcattgg ggtgaaactc gagtggatca gaagaaagtc gaagcggttg gcgttcagtt
     6061 gcttatgtca gaaagattaa agaggataaa agaaaaagaa tctcctattg actagataga
     6121 agattctccg atctctttag taatagcgtt actaaaggga tcctacagac aaaaaaatga
     6181 ttattaacga atgtacttgt attatacatt taaagaatat tttcgtctat tttaatagtt
     6241 ggtttcatgt ttctactggg ggctagtaac accccagtag aaaccccatt caaaaccggt
     6301 ctggtccctt gtggctctag ggttcgtccc tctttgtttt ttttacggtt cttttaaaaa
     6361 aacaggcaaa aaatttgtgg tttttatttt tttatattgt attcattgga gtggtggatg
     6421 aaaaaagtgg agaattataa aagaagaaaa cctcactgtt gaaagtattg aggaaaatag
     6481 tattttagca aatatttatt tgtaatcctt ttgaatgttt ttatcattta gaaggttttt
     6541 ttatagtttt tcggttacga aaaatcgggt ttcttctttc tttaatacat aggtaactat
     6601 ttccgcacgg aaattgatgc ctatgtatgg gtttagccct tgtgtatcaa gggtttgcaa
     6661 aaaaaaagac ttgcttttct aggcggatag atttaatgtt ttaagtgact agctaaaaac
     6721 aaaatcatcc aagaaaggag caagtccgaa aaaaggggtt cgtgtaccct tttttatgaa
     6781 attcagatag gactaatata tcaatatggc aaagaaaaat caagatgaaa tcactggtaa
     6841 actacaacct aaaaagaaac agaatataaa aatatacgaa tttatagaga gaaaaatgag
     6901 cgagagtggt agagaattgt ttaaatcatg tagcactttt aatgagtttg tggctacaaa
     6961 ggataaaaag aaaaagaaac gagtaaaggg gaatgattgt aaaaatcgtt tttgtccgat
     7021 ttgtgcttgg aggaaagcag gaaaggacgc agttaaaata gcaacgatga tggaagcgat
     7081 taagattgag gaaaagaaag agtttctttt cctcacattg acgacgccga atattaaagc
     7141 tgatatggta aaaagtgaga ttgatagatt taataaggct tttaataaat tatttaagag
     7201 acgtaatatc caacgttcta ttaaaggtta tattagaaag ttagaaatga cgtatgataa
     7261 agaacgattt attacaaagg gaatgtataa agatagaaaa gcatattatg ataaacgtgg
     7321 attgaaagtg gacgatcata atccgaatta tgatacatat aatcctcatt ttcatgtgtt
     7381 actttgtgtt gataaaaatt atttcaaacg taaagaactt tatattaagc aggaagaatg
     7441 gttggaaatg tggcgagaag taacagatat gccagagatt acgcaagtac atattcaaaa
     7501 agtagagtta ataagagaag gaaatgcggt tgcggaagtt gcgaagtatt cggcaaaaga
     7561 ttatgaaatg tcagtgagtc aagatgtatt tgatgtgttt tatttagctt taaaaggtcg
     7621 gcaattaatt gtgtatggtg gattgttaaa agattatgcg aaaaaatatg aagatggcga
     7681 attggacaag tataagagta aagataagaa tgagtattat tatagattaa tagcaatatg
     7741 gaataaggat ttaatgaaat ttgaacaaga gtatcaagag ttaacagaga gtgagaagca
     7801 agaatataac ggtcatttga ttgatgaagt agatgtggag gcgtaatata aaaaaagaaa
     7861 aaagagtatt agaaaaaaag taagggagct ggctgttgta ttaagtcagt ttcctttttt
     7921 tatttacagt tagtgttaaa attttaatgt ataaaatgta ggatgtatag gtaaaatttt
     7981 gtagaggtgt aataaatggg tagaaagcgt attgatccag atgaaaaaaa gactagattg
     8041 aatgttgcta ttaagaaaaa atatgttgat ttagttaatc aagaagaaaa tacatcccaa
     8101 tttgttgagg atgcaatagt gaagtatttg aaagaaaata aaaagattta attttttttt
     8161 ttggaaaata cttgttttct gttccctttt tttgctataa tcagaacgta aaatcttgct
     8221 acgaataaat gttcgttcag gatatttgat tttatattat attttttcat aaaatttaaa
     8281 aggagtgtaa ttttatgcca caagcaatta ttgaaggaca atacttaagt tcaagtatta
     8341 aaaaatcaac ttttaatggt gttgaaaaat catttgtaca attggatgtt tatcagcctg
     8401 aaagtactga tagcgaaaaa acagttgttg ttaagtgtga tgatttagag cttattaatc
     8461 attttaagga tacgaaaatg gggatgccta ttaaagcaaa ggtttctatt aatgcttatc
     8521 aaaataaagc atattttaaa ttggttaatt tagcgtaaaa tctttatatt aagacaaaaa
     8581 aatgaatgtg aatgaatgtg attttatgtt ggataaaatc gatttaaacg aattgtacaa
     8641 aaccatttcg gaaattatgc ctaactactt agtacagact aagaacggta gttttgcagt
     8701 tttccagaca cttacctatg gtgatatgtt aattagtttc ttgttattgc taatcttgtt
     8761 ggtgttgaca ttcaactgga tatggagtgc tttacgatga tcgagtttaa tttagaaaac
     8821 ttgttgaatc aatattcctt gtttggatta ccatttttat taattatcgt gttcacattg
     8881 ctgaaagatt ggcggtttga tagataatga atgatttagc gtttctagat atgttgtatt
     8941 ggttcttcat gaataattca gttgtattat attcgttagg ttttataacc tttcttgtgt
     9001 tcgtaataat gatgttcacc accgtcaaag cttggggtga tggctattag ttttagcatg
     9061 gacgtgggaa gaatcttgca gtatgccttc aacatgttta gttcagtgtt accagtgatt
     9121 tatttattca ttggtggggc gtttgctatc tactgtatcg gtaaagtgta caacaccttt
     9181 aggagttgat ttcaatgcct gaatttggat tcagaccatc gatgtctcaa ttattcggta
     9241 actttcatat gaattggtca acaatgatgc cattgttagt cattttgttt gggatactgt
     9301 tcggattttt cgtagcgcat ttagtaaaag acaaatttga tgattgatta actaggagga
     9361 atttattttg gaaatgccaa aaatttcatt aacttataat cttgcagatg ttgcaaatgg
     9421 tttaactaac tggttaggcg cttactggcc tattatcgca ttctctgttg ctattccgtt
     9481 aacgttctta gtagcgttta acactaaaga tttatttact cgttaatcat caaaaatcac
     9541 tccttaaata gcaagaagtt aactataaat ttaataaaac aatcaaatag ctgggtggat
     9601 cgcctagcta ttttttgtta ggaaggattg atatgaaaaa agcagccata atttttttat
     9661 gtacattttg tattattttt cgtacagttc ctgtctttgc tgatacgcca tcatttcaaa
     9721 tggtaggaag taaaggtgtt tccatagaaa ataaagggct tgttggcggt tattatagtc
     9781 tttcgttaaa gattagtgat gctaaagacg tagtgacgtt tcaattgagt gagttgatgc
     9841 tcgttaatga ttgtagtttt aattttggac tagtgaaatg gactaggccg tcacaagaga
     9901 agaatgagtt tgatattaaa gtcaaagaaa atggaacata tgctttttac gtgtttgtga
     9961 atgggaaagt taaaggatat gttcgtttta aaattaaagg atttcctaaa aatgctgaag
    10021 gtgctggagt aactcaatat tgggggcaag gtgcaaagta ttatgaactt cctgaagaac
    10081 atgtaaatcc tgaggatttt cgagacaaag atggagaaaa taatggaatt tgcgtgaatg
    10141 aaaaaatttc tgatgataaa ccaggtggtt caggagatat agaagaacca aaagaggata
    10201 ataaaccgcc agttgaaggc ggtggtggtt ctgataaagc tattaaagaa gctttagata
    10261 aaattacaag tgcagtaaat gaaatttcaa agaatacggg tgaaacggct aaaaatacag
    10321 gtgagattaa aaagaacacc gatgaaatag caaagaacac aaaagaaatc gcggatgctg
    10381 ttaaggatat tagggataat ttgaagattc ctgacgatat ggatttgtcg attaaacctg
    10441 ttcttcctga ggacataaag cctgataaac ctaaacaacc tgataagcct tttgaagaca
    10501 aacaagagca ctttaaagag ggtgaacaag agaaaccacc tgacaaactt cctgttgctc
    10561 ctgagcccgc cgaatgttgg ggggatgttt gtaaagataa agataatcag aaagaagaag
    10621 atatgaagaa agataaagat atggataaag aagaaccaat gaagcctgat aaaccaatgg
    10681 aaaaggaaga catgaaacca gataagccaa tgactcctga taagccaatg gaaaaggaag
    10741 acatgaagcc cgataaacca atgactcctg ataagccaat ggaaaaggaa gacatgaagc
    10801 ctgataaacc aatgactcct gataagccaa tggaaaagga agacatgaag cctgataaac
    10861 caatgactcc tgataaacct atggatcgag agggagaaat gaaaaaagat aatgatatga
    10921 aacaagataa gccaatgact ccttgggaag aaatgaaacc aagtgacgaa gggagtgaac
    10981 tttgttggca tgtagtaaat ggaaaatgcg gataaagaaa tttttattag tagtgccttt
    11041 attacttgga attttattta taggtgatac aagtgcattt gctgaggttt ctcgtgcaag
    11101 tttaggaaat gatttgctga agggtgtatc aggtagtatg tgtggttcta cagtaaccaa
    11161 gataactgat ggtaatcctt caacaggaga aactttgttt aagacttgtc gggaaattcg
    11221 atttaaatta cctaaagtat caaatgtatc aggtttttta tatataaaaa caaataagtg
    11281 gactagtgat aaattggcta ttgattttaa aaaagtagat ggtcaagtta aaactattac
    11341 gtataatcag gcttctgtta attcaaataa ttatattcaa gtatttgtta aggacgtttc
    11401 tgaagtagtg ttgaggaatt ttgatgatac aggtgcaatt gagattatgt catttgaatt
    11461 atatgagggt aacggagatg aattttttta tgaggatata aaagaattac agccgactat
    11521 tacatatgat tctgttactt tttcttataa gaatcccaaa atagattttg gttatttaag
    11581 aatttataga gatggtaagt tgattgttga taattttaaa ttagagcaat ttaaagataa
    11641 ggaattaagt cctaacactg aatacaaata taaatttgtt gctgtatcat ctgatggtat
    11701 tgaatctaag ggcatagaaa aagtaattaa aactgatgaa aagcctaatt tagctaaacc
    11761 aattaaacca tctaatccta cgataacacc taaagatggt tctctaattg ttaatttaac
    11821 gaattatgat gctggtgtga aagtaaaggg ttatcacatt gttgtagatg gaaaacaggt
    11881 aaatgattca ttggttacag cacgatcata tgcgattacc ggtttgaaaa atggacaatc
    11941 atatcaagtt cagattaaag ctgtatcggc ttggaatata gagagtgatt taagtaattc
    12001 tgttccaggt attccacaag tgcaagtaat acctgatatc gcttttaatt ttggtctttc
    12061 tgatttaatg gaatctatta aaaattggtt cggtggaatt tggcctatcg ttgcttttag
    12121 tattgctatt ccattaacgt ttattgttgc gtttaacacg aagaagttat ttttaaggta
    12181 ggtgcttagt ttgatacaac cagttacatc taaggactta atacaatttc tccagtatgc
    12241 atttcatgct ggggaaaacg tattatttct agcgtttgta ttaatgatta cattctctgt
    12301 tgctataagc attagaagga ttatggtggg ttataagcaa tcatgatgga gaagtttgtt
    12361 aagttcttaa aaggtggatt tgaagcggtt ataaagtcgt taaaaacgtt tattgatttt
    12421 ttagctacac catttcaata tctattggat tttttaaaag gaatctttta tttcatagaa
    12481 aagctgtttc aagttgtagt agcaattata aaattattcg ttgccttgtt tcagttttta
    12541 ggtgctgtaa tagcgggatt ctttaaaact ttatttctat gggtacaacc taattttgat
    12601 ggtgggaatg tatcttttcc tagtgcttcg atgaagggat ttaagacagc gacagatata
    12661 ttacttccta ctggtttgtt aacagtagtt ccgatggttg tcacatgttt tatttggtac
    12721 ttgtttg
//
