LOCUS       NZ_CP083163            14553 bp    DNA     circular CON 11-APR-2024
DEFINITION  Bacillus thuringiensis strain ABTS-1857 plasmid pCH_181-g, complete
            sequence.
ACCESSION   NZ_CP083163
VERSION     NZ_CP083163.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214332
            Assembly: GCF_020809245.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 14553)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14553)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x, 40x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809245.1-RS_2024_04_10
            Annotation Date                   :: 04/10/2024 23:39:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,957
            CDSs (total)                      :: 6,806
            Genes (coding)                    :: 6,416
            CDSs (with protein)               :: 6,416
            Genes (RNA)                       :: 151
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 175 of 390
            Pseudo Genes (incomplete)         :: 226 of 390
            Pseudo Genes (internal stop)      :: 99 of 390
            Pseudo Genes (multiple problems)  :: 87 of 390
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083163.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14553
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="ABTS-1857"
                     /isolation_source="Biopesticide XenTari"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_181-g"
                     /collection_date="2017"
     gene            311..724
                     /locus_tag="K8Z20_RS34370"
                     /old_locus_tag="K8Z20_33975"
     CDS             311..724
                     /locus_tag="K8Z20_RS34370"
                     /old_locus_tag="K8Z20_33975"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021729666.1"
                     /translation="MKKILFFILTIVMISTFSLPNKYVQAAEISKQPSLDTYLTPEIEK
                     EINNNMQPATKENPISSYTASNGLKVVDTVKTEPAFSDSFSSTSYVTHSKTFYASGLSI
                     ATYKGKWLCTFWRDGSVTIKNWDYFLVGIKGYV"
                     /name="hypothetical protein"
     gene            1302..2156
                     /gene="tnpI"
                     /locus_tag="K8Z20_RS34375"
                     /old_locus_tag="K8Z20_33980"
     CDS             1302..2156
                     /gene="tnpI"
                     /locus_tag="K8Z20_RS34375"
                     /old_locus_tag="K8Z20_33980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnP I resolvase"
                     /protein_id="WP_000382147.1"
                     /translation="MDVAKQFSSYLKQENKTENTVQGYTSGIRQYIKWFEGSYDRKLTK
                     LYRQNILEYISYLKNVKMLNAKSINHKISSLAKFNEFLIQKGSQQDQVILKTDMIKVQT
                     VYASPTQIVELDVKKFLQSVLEDNNKRNYAIATLLAYTGVRISEALSIKMNDFNLQTGE
                     CIIRSGKGGKQRIVLLNSKVLSAIKDYLIDRKTYSTAHESPYLFISKKREKLDRTVVNR
                     IFKSYSNVITPHQLRHFFCTNAIEKGFSIHEVANQAGHSNIHTTLLYTNPNQLQLKNKM
                     ELL"
                     /name="tnpI"
     gene            2175..5138
                     /locus_tag="K8Z20_RS34380"
                     /old_locus_tag="K8Z20_33985"
     CDS             2175..5138
                     /locus_tag="K8Z20_RS34380"
                     /old_locus_tag="K8Z20_33985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538378.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_000538375.1"
                     /translation="MGVKQLLSEAQRNELMDLSRLTEWDLVTFHTFSKHDLHLILKHRR
                     GYNRLGFALQLALIRYPGWSLTEYKDIPQYVVAYVASQLQIPPEEFLVYAKRGNTLWEH
                     LGEIRTEYGYQNFSSEYKETLLQFLVQQAMDNNNTLYLIEITISTLRKMKVILPAMYVI
                     EDIVWEAKQQADQKVYSILHDGLVQEQKDQLDALLLPTINGKSPLAWLKDVPAQPSPES
                     FLKVIDRLQFVQKIGLTIDTTKINTNRLRQLARLGSKYEPYAFRRFNEVKRYSMLVSFL
                     LEITQDLIDYAIEIHDRLMMNLQTKGKKEQDEIQQANGKKLNEKILQFITVCGTLIEAK
                     ETGKDAFAALDEVMSWNEMVESVEEAKQLSRPLNYDYLDLLNTRYSYVRRYAPTLLRSL
                     HFRATKSGEPVLQALDTIHELNETGKRKVPHGAPLHFVSNRWQKHVYDDDGNINRHYYE
                     LAALTELRNHIRSGDIFVSGSRHHKAFDDYLIPYDEWNEVSNIPNGLTAPLKAEDYITD
                     RINRLNEHLEWLSKNSEKLEGVDISQGKLHVERLDRGTPEEAKAFSKLLHSMLPRIKLT
                     DLLIEVASWTGFHDQFIHASTNQSPDQEEQNIVLATLMAMGTNIGLTKMAEATPGISYR
                     QMANASQWRMYDDAMVRAQSILVNFQKEQKLSSYWGDGTTSSSDGMRLSIAVRSLHADS
                     NPHYGTGKGGTIYRFVSDQLSAYHVKVITTNARDALHVLDGLLHHETDLKIEEHYTDTA
                     GYTDQVFALTHLLGFRFAPRIRDLADTKLFSIPGGEEYENVQALLKGKINVKLIKENYE
                     DIRRLAYSVQTGKVSSALIMGKLGSYARQNKLATALGEMGRIEKTLFTLDYISNKAVRR
                     RVQKGLNKGEAINALARIIFFGQRGEFRERALQDQLQRASALNIIINAISVWNTVYMEK
                     AVEELKARGEFREDLMPYAWPLGWEHINFLGEYKFEGLHDTGQMNLRPLRIKEPFYS"
                     /name="Tn3 family transposase"
     gene            5295..6731
                     /locus_tag="K8Z20_RS34385"
                     /old_locus_tag="K8Z20_33990"
     CDS             5295..6731
                     /locus_tag="K8Z20_RS34385"
                     /old_locus_tag="K8Z20_33990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558040.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS231C family transposase"
                     /protein_id="WP_001053969.1"
                     /translation="MNLSIQDELQLFSEELYRHLTPSLLEELAKELGFVKRKRKFSGNE
                     LATICIWVSQRTASDSLVRLCSQLHAATGTLMSPEGLNKRFDKKAVEFLKYIFSALWKS
                     KLCKTSAISSAALTYFQRIRILDATIFQVPKHLAHVYPGSGGCAQTAGIKIQLEYDLHS
                     GQFLNFQVGPGKNNDKTFGTDCLDTLRPGDLCIRDLGYFSLEDLDQMDQRGVCYISRLK
                     LNHTVYTKNPFPEYFRNGTIKKQSQYIQVDLENIMHTLKPGQTYEIKESYIGKNQRLFT
                     RVIIYRLTEEQILERRKKQSYTESKKGITFSEKSKRLTGINIYVTNTPWEVVPMEQIHD
                     FYSLRWQIEIIFKTWKSLFQIHHWQTIKQERLECHVYGKLIAIFICSSTMFKMRQLLLQ
                     KHKRELSEYKAIGMIQDHLSLLYQAIQRNTRIITKVLIRLFTLLKKNGRKSHRYEKKTI
                     FDIMGVVYEYNGLRKQKKAA"
                     /name="IS4-like element IS231C family transposase"
     gene            complement(7901..9238)
                     /gene="mobV"
                     /locus_tag="K8Z20_RS34390"
                     /old_locus_tag="K8Z20_33995"
     CDS             complement(7901..9238)
                     /gene="mobV"
                     /locus_tag="K8Z20_RS34390"
                     /old_locus_tag="K8Z20_33995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031674.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_001031676.1"
                     /translation="MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNY
                     DLLNQQKIRYESTIKQEISERVKRKPRANSVVLSEFVVTASPDYMHSLSLEEQKRYFES
                     SLDFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPITEDNRLSAKDMFTRKELISLQQDF
                     PLEMREKGFDVDRGEGSEKKHLSPQAFKEKQDLEVEVEQLSNVKTHLKTKVVETHNQLQ
                     QTTNYIEKQNETLQKIQQQFLSLDKKIKEKKQEFETFRNQIPDKPVSMSYLREETKTEV
                     TTKLFGKPEITEKKTGNIVVTREQWRDMTEKVNAAVIVKKDYERLQKTDLVKENQSLRE
                     DNKYLEETIEGNNLALKHSYKQNRELEEVNKELHTEIGTLKAHIRDLQMNIKVLYQQTK
                     KVFKEQFKAFRGLIKNELDMKGVDNQFEREHTREIRSRQKGYDMER"
                     /name="mobV"
     gene            complement(9840..10190)
                     /locus_tag="K8Z20_RS34395"
                     /old_locus_tag="K8Z20_34000"
     CDS             complement(9840..10190)
                     /locus_tag="K8Z20_RS34395"
                     /old_locus_tag="K8Z20_34000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012094734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="WP_021729366.1"
                     /translation="MLIMMTIFISIVLVVSFVLFNRVHAKKSIYNYIARQGIQESQLKY
                     IDFRKDFKIGGYWLAVYVEGENPDIYYEYSYQDKKVNFQAYFNSEKAIKKKMWGGSGLT
                     ETEMKKLKYPPL"
                     /name="DUF3139 domain-containing protein"
     gene            complement(10228..11040)
                     /locus_tag="K8Z20_RS34400"
                     /old_locus_tag="K8Z20_34005"
     CDS             complement(10228..11040)
                     /locus_tag="K8Z20_RS34400"
                     /old_locus_tag="K8Z20_34005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000734782.1"
                     /translation="MKKLLISFIAILFFICGFNLKAFAAEEVIDYQSLYNQAIQEGVLD
                     QNSVSYNEWLKQNKEEFMPIYQDGLKQGVFLEPLSYNEWLKLNNYGQAPMGDTEFFDDV
                     TPKGSWGGFTLKAGDIFITNATSSAGIVGHAAIANGDNYILHMPGAGQGNQQLSTSNWM
                     QKYTASGKWIKVYRLKDQTLARDVARYADRNFYSTTGSATKNVYLDYGIDTHLYQKNPT
                     YCSKLVFQAFYFGSGSRNVMQAVSGIVTPYGLIDTFTSAYRPSLVKTY"
                     /name="hypothetical protein"
     gene            complement(11617..12228)
                     /locus_tag="K8Z20_RS34405"
                     /old_locus_tag="K8Z20_34010"
     CDS             complement(11617..12228)
                     /locus_tag="K8Z20_RS34405"
                     /old_locus_tag="K8Z20_34010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000185570.1"
                     /translation="MTNTIDFKQVEKNARIRDFENEKEKFKQDHNGINQEEINQAMQVL
                     SKATGGKEVFIGTKRSPQSRVKFAQFIQDNIDQLEEINYLDNKEIVVIWKISRKIGFLS
                     NCIVDDIHKKNPVPLTQTEIALLLKRTKNNINPIIKSLVDKGILAVSEIGIAGNNVRAN
                     AYFMNPNIIFAGNKDDVNPTLKAMFHRVPKELKNLPVKLF"
                     /name="hypothetical protein"
     gene            12689..13780
                     /locus_tag="K8Z20_RS34410"
                     /old_locus_tag="K8Z20_34015"
     CDS             12689..13780
                     /locus_tag="K8Z20_RS34410"
                     /old_locus_tag="K8Z20_34015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RapH N-terminal domain-containing protein"
                     /protein_id="WP_001102654.1"
                     /translation="MNVQLENEQITKLLNDWYQSMLKQQLVKAKQLKEYIDSEINNVKE
                     NQNLLLYYSLLDFRYKALTDWISINENSFDEMDNFTTPADDFLAYYYHFFKAFHSTLTS
                     NYTEASEHYEKAKELLIHIPDPIEHAEFNYRMGYFYYQIYKQVIALDYIKLAKEEFSKH
                     EGYEINIALCDNFIGLCCIDLKHFELAEESFNKALDTFQKNNHKNSMLMVRHNLAWLYS
                     KQNLSKLAIRHVSEVTKNNPNHFKAFFVEAFEYYKINDHNYAEQLIEKGLKLSNDFNNQ
                     EFQHRFKILKALNNKVPILTLETSISEGITYFKQEKLWECVKEYADILALRFYEENNHN
                     KASQYFYMSNTAQKNELEKGALK"
                     /name="RapH N-terminal domain-containing protein"
     gene            13780..13938
                     /locus_tag="K8Z20_RS34415"
                     /old_locus_tag="K8Z20_34020"
     CDS             13780..13938
                     /locus_tag="K8Z20_RS34415"
                     /old_locus_tag="K8Z20_34020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000758643.1"
                     /translation="MKKVYLTILGISLISILSLGLNDSFKNQAVNIGDGGGAPARPDYF
                     LQTHSNR"
                     /name="hypothetical protein"
     Typing          complement(7905..9238)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.101"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaacgtatt ttaagaacta taacatatat accaattgca attaaagtac accaaatcaa
       61 tgaaatcatg ttaaaaaaca acatcaatat tacatttgtc aatattacag ataaaattcc
      121 aaaaacattt ttattactag aaattaaatt ataaacaact aaaccaatta tcgttgaaat
      181 aatcgtacaa aaaaggatca atataaaata catacaaata aacctccttt ttacatttct
      241 gtctttattt tctaaatatt ttgtattatc ataataaatg taccaaataa taatatcaag
      301 gaggacaatt atgaaaaaaa ttttattttt catcttaaca attgtaatga ttagtacatt
      361 ttcattacca aacaaatatg tgcaagctgc cgaaatttcc aaacaaccta gcttagatac
      421 atatttaact cctgaaattg aaaaagaaat taacaataac atgcaacctg caacaaaaga
      481 aaatcctatt tcatcttata cagcttctaa cggcctaaaa gttgtagata ctgtgaaaac
      541 agaacctgct ttctctgatt ctttttcttc aacatcatat gttactcatt caaaaacttt
      601 ctatgctagt ggactttcca ttgctactta taaaggaaaa tggttatgta ctttctggcg
      661 agatggtagc gtaacaatta aaaactggga ttatttctta gtaggaataa aggggtatgt
      721 gtagcaatgg aacaggataa cgcaacaagc attagcggac atcatatggt atagcgactc
      781 cattaagtta actgagttaa cctgagttat aataagggtt tttgtgtgca tttaggttaa
      841 cttaaaaagt cgatgtcatt tgagcacaac tagataaatt tgatacttaa tgtcctataa
      901 gtatcattag cggacattaa ctcagtaaaa atagttattt tatgtccgct aatacttatt
      961 gcgtgatttg gttccattgc tacacaaaga ccatacttat tgcgtgattt ggttccattg
     1021 ctacacaaag accataaacg atggttccta tagtaggggt accgccagca tttcggaaaa
     1081 aaaccacgct aagaaaatca gagttaaaaa atcagaaaat atatcattat tccaagacac
     1141 atacagtgtc tttttttata caaaaaataa tacaacacaa tattaattgt gttgtattag
     1201 gtgttataat aaatataaat ctaggggttt aacgcaacac aatttatcga taaataaata
     1261 cttttagacg caacacaatt tatagacgcg gaggaaatca catggatgtt gcaaaacagt
     1321 tttcttctta tcttaaacaa gagaataaaa ctgagaacac tgttcaggga tacacatcag
     1381 gtattagaca gtacataaaa tggtttgaag gttcctatga cagaaaatta acaaaattgt
     1441 accgacaaaa tatcttagag tacattagtt atttaaagaa tgtcaaaatg ttgaacgcca
     1501 agtccattaa ccacaagatt agtagccttg ctaaatttaa tgaatttcta atacagaaag
     1561 gaagtcaaca agatcaagta attttaaaaa cagacatgat aaaggttcaa actgtctatg
     1621 cttctccaac ccaaattgtt gaattagatg taaaaaagtt tttacaaagt gtgttagagg
     1681 ataataacaa acgtaattat gcaattgcca ctctcctagc atatacagga gtacgtattt
     1741 cagaggcatt atctatcaaa atgaatgact tcaatttaca gactggggaa tgtattattc
     1801 gaagtggaaa aggaggtaaa caacgaattg tattactaaa tagtaaggta cttagtgcta
     1861 tcaaagatta tctcatcgat cgaaaaacat acagtacagc acatgaatct ccgtatcttt
     1921 ttattagtaa aaagcgagaa aagctcgacc gtacggtcgt caatcgtatc tttaaatcat
     1981 acagcaatgt tattactcca caccaattac gacacttctt ctgtacgaat gcaattgaaa
     2041 aaggatttag cattcatgaa gttgcaaatc aagctgggca ctctaacatc catacgacac
     2101 tactttacac aaatccaaac caactgcagc taaaaaataa aatggagctc ttataaagga
     2161 gtaaagggga aacaatggga gttaaacaat tactgtcaga ggcacaacga aatgagttaa
     2221 tggatttatc acgtttaaca gaatgggatt tggtaacatt tcatactttt tctaaacatg
     2281 acttacattt aatattaaaa caccgtagag gatacaatcg tttagggttc gctcttcagc
     2341 ttgcattaat tcgttatcct ggctggtcct taacagaata taaagacatt ccacaatatg
     2401 tagtggctta cgttgcgagt cagttacaga ttccacctga agaattttta gtatatgcca
     2461 aacgtgggaa tactttatgg gagcatcttg gggaaattcg aacagaatat ggatatcaga
     2521 atttttcttc tgaatataaa gaaactttat tacaattttt agtgcaacaa gcaatggata
     2581 acaataatac cctctattta attgaaataa caatatctac attgcgaaaa atgaaagtca
     2641 ttcttccagc tatgtatgtg attgaagata tcgtctggga agcaaagcaa caagcagatc
     2701 agaaagtgta tagtatacta catgacggtt tggtccaaga acaaaaagat caattagatg
     2761 cgttactttt accaactata aatggtaaat ctccattagc atggctaaaa gatgtaccgg
     2821 cccagccttc accagaatca tttttaaaag taatagaccg attacaattt gttcaaaaaa
     2881 ttggtcttac tattgatacc acaaaaatta atacaaatcg tcttcggcag ctcgctagat
     2941 taggatcaaa atatgagcca tatgctttta gacgattcaa tgaagtaaaa agatatagta
     3001 tgttagtctc atttttattg gagattacac aagaccttat agattacgca attgaaattc
     3061 atgatcgtct tatgatgaat cttcaaacaa aagggaaaaa agaacaagac gaaatacagc
     3121 aagcaaatgg gaaaaaatta aacgagaaga ttttacaatt tattactgta tgcgggacac
     3181 taattgaggc aaaagaaacg ggtaaagatg cttttgcagc cctagatgaa gtaatgtcat
     3241 ggaatgagat ggtcgaatct gttgaagaag caaaacaact atcacggcct ttaaattatg
     3301 actatttgga tttgttaaat actcgttatt cctatgttag acgatatgca cctactttat
     3361 tacgcagtct tcattttaga gcgacgaaat ctggagaacc cgttttacaa gcacttgata
     3421 cgattcatga attgaatgaa acaggtaaac gaaaagtgcc tcatggcgct cccttacact
     3481 ttgtatctaa ccgttggcaa aaacatgtat atgatgatga cggaaacatt aatcggcact
     3541 attatgaatt ggcagcttta accgagttaa gaaatcatat tcgttcagga gatatttttg
     3601 tatcaggcag tagacatcat aaggcatttg atgattatct tataccgtat gatgaatgga
     3661 acgaagtttc aaacattcca aatggcctta cagccccttt aaaagctgaa gattatataa
     3721 ctgaccgaat aaaccgatta aatgaacatt tagaatggtt atcaaaaaat agcgagaaat
     3781 tagaaggagt agatattagt caaggaaaac tacatgttga gcgattggat agaggaactc
     3841 cagaagaagc aaaagcattt agtaaacttc ttcatagtat gttacccaga ataaaactga
     3901 cagatttact aatagaagtg gccagttgga ctggatttca tgatcaattt atccacgcct
     3961 ctaccaatca atctcctgat caagaggaac agaatattgt attagctact ttgatggcaa
     4021 tggggacaaa tataggtctt acaaaaatgg cggaagctac ccctggaatc tcctatagac
     4081 aaatggcaaa tgcttcacaa tggagaatgt atgatgatgc aatggttcgc gctcaatcta
     4141 ttctcgtgaa tttccaaaag gaacaaaagt tatcatccta ttggggagat gggacaactt
     4201 cttcttctga tggtatgcgt ttatccattg ctgtacgttc tctacatgca gattccaatc
     4261 cacactatgg aacgggaaaa ggtggaacca tttatcgatt tgtaagtgat cagctatctg
     4321 cctatcatgt gaaagtcatt actaccaatg cacgagatgc tcttcatgta ttagatgggc
     4381 ttcttcatca tgaaacagat ttaaaaattg aagaacatta cacagataca gctggatata
     4441 cagatcaagt tttcgcttta acacacttgt tgggatttcg atttgctcct cgtattcgtg
     4501 atttggcaga tactaagctt ttttctatac ctggtggaga agaatatgaa aatgttcaag
     4561 cacttttaaa aggaaaaatt aatgtaaagt taataaaaga aaactatgaa gatattagaa
     4621 ggttggcata ctcggttcaa acaggaaaag tatctagcgc tcttatcatg gggaagctcg
     4681 gatcatatgc aagacaaaat aaactcgcaa cagcacttgg agaaatggga cgaatcgaga
     4741 aaaccctctt tacgctggat tacatatcta ataaagccgt aaggagacgg gttcaaaaag
     4801 gtttaaataa aggagaagca atcaatgcat tagctagaat tatatttttt ggacaacgtg
     4861 gagaatttag agaacgtgct ctccaagacc agttacaaag agctagtgca ctaaacataa
     4921 ttattaacgc tataagtgtg tggaacactg tatatatgga aaaagccgta gaagaattaa
     4981 aagcaagagg agaatttaga gaagatttaa tgccatatgc gtggccgtta ggatgggaac
     5041 atatcaattt tcttggagaa tacaaatttg aaggattaca tgacactggg caaatgaatt
     5101 tacgtccttt acgtataaaa gagccgtttt attcttaata taacggctct ttttatagaa
     5161 aaaatcctta gcgtggtttt tttccgaaat gctggcggta ccccatgccc atcaacttaa
     5221 gaatttgcac tacccccaag tgtcaaaaaa cgttattctt tctaaaaagc tagctagaaa
     5281 ggatgacatt ttttatgaat ctttcaattc aagatgaatt acaactattt tctgaagagc
     5341 tgtatcgtca tttaacccct tctcttttgg aagaactcgc taaagaatta ggttttgtaa
     5401 aaagaaaacg aaagttttca ggaaatgaat tagctaccat atgtatctgg gtcagtcaac
     5461 gtacagcgag tgattctctc gttcgactat gcagtcaatt acacgccgcc acaggcactc
     5521 ttatgagtcc agaaggactc aataaacgct ttgataaaaa agcggttgaa tttttgaaat
     5581 atattttttc tgcattatgg aaaagtaaac tttgtaaaac atcagccatt tcaagtgcag
     5641 cactcacgta ttttcaacga atccgtattt tagatgcgac gattttccaa gtaccgaaac
     5701 atttagcaca tgtatatcct gggtcaggtg gttgtgcaca aactgcaggt atcaagattc
     5761 aattagaata tgacttacac agcggacagt ttttaaactt ccaggtaggt cctggaaaaa
     5821 ataatgataa aacatttgga acagattgct tagatacctt acggccgggt gatctgtgta
     5881 ttcgtgattt aggatatttt tcattggaag acttagatca aatggatcaa cgtggcgtgt
     5941 gctatatatc aagacttaag ttaaaccata ccgtctatac gaaaaatcca tttccagaat
     6001 actttcgaaa tgggacaatc aaaaaacagt cacagtatat ccaagttgat ttagagaaca
     6061 ttatgcacac gttaaaacca ggacaaacgt atgaaataaa agagtcgtat attggcaaga
     6121 atcaaagatt atttacaaga gtaattatct accgattaac agaggaacaa atactggaac
     6181 gtagaaaaaa acaaagctat accgaaagta aaaaggggat tacattttca gaaaagagta
     6241 aacgattaac gggtatcaac atatatgtta cgaatacgcc ttgggaagtg gttccgatgg
     6301 aacaaatcca tgatttttac tccctccgct ggcagatcga aatcatattt aaaacgtgga
     6361 aatctctatt tcaaattcat cattggcaaa ctatcaaaca agagcgatta gaatgccatg
     6421 tgtatggaaa actcattgcc atttttatat gttcttccac gatgtttaag atgcgccaac
     6481 ttctgttgca aaagcacaaa agagaactaa gcgaatataa agcaattggg atgattcaag
     6541 atcatctatc cctgttatat caagcgatac agagaaacac ccgtataata acaaaggttt
     6601 taatccgcct gtttacccta ctaaagaaaa atggccggaa atcccatcga tatgagaaga
     6661 aaactatctt tgatattatg ggtgttgtct atgagtataa tggattgaga aaacaaaaga
     6721 aagctgcata agaaaaaaat gaaacccgtt agggtttatt tcgtatgctc atttttaaga
     6781 ggattatact gtttaaaagt ttgttcattt caattaatgg aaaaccgtag agtttctatc
     6841 cttaagttga tgggcatgtg gcggtacccc tagtaataaa tctactagta tactatctag
     6901 tggtaaaccg gcaaaagctc gtggatatgt aacacttcaa agtggattct tcacaggtga
     6961 tacaatctgg aatataagta tttctaatgg ttcttctgta tctagttcaa ttacaaaaac
     7021 aagataacaa aagccacatt aatgtggctt tttatttttc aataaataat gttttttacc
     7081 atagtataaa atcccgttta atgccttcct tctgtttgcc cccacttcgc aaacagactg
     7141 gctagtccga ataggctaac acaggcgcct ttccttactg cgaagactaa ggggcaaagg
     7201 tctggtgtgg aaatcctttt attcttgtgt tctgaatgta tactttcttt atggaaatgt
     7261 ctatttttct aaaaatctct taaagttcta taaaatcaat aatttgaaag taaactaata
     7321 ttcaaaagtt tactttggaa cttacaattg aaattcaaca tacaatgcta tgagcattta
     7381 caacatagtc ttggtcgaca ataaaaaaag tacgaagtct tttaaaagac ttcgtactaa
     7441 caacagacta aagtacagtt catcggaaac tgaaagggta tttcatctat ttattataaa
     7501 tcattaattc ctatttccgg gaattaatta gaaaatatgc aaaatttcat gttttataat
     7561 tccttataaa caacaaacct agtgaatatt tttgaatttt ctttttatta atgttagact
     7621 aaattatata agatacaact ccttaaacgc ctagatatat ctctattcgt tatacgcaaa
     7681 aaaaccaatt ctcgtgtaat agaattggtt tttttgtgca attcagtcaa acagcaactt
     7741 gaacccacat tttcaaatgt agtcttatca atcttaacat aattttaatt acataaacgc
     7801 aaaaaatctc tttatatgca cacatataaa gagattttta aatggatgaa acccataaat
     7861 tagttagtga tgaataatta tagtatatca caaaacgttt ctaacgttcc atgtcatatc
     7921 ctttctgtct acttcttatt tctcttgtat gttcacgttc aaattgatta tctacaccct
     7981 tcatatccag ttcgttttta ataagtcctc taaatgcctt aaattgctct ttaaagactt
     8041 ttttcgtctg ttgatataaa acctttatat tcatctgtaa atctcttata tgagccttta
     8101 aagtgcctat ttcagtatgc aattctttat ttacttcttc taattcccga ttttgtttat
     8161 aagaatgttt taaagcaaga ttattcccct ctattgtttc ttctaaatat ttattatctt
     8221 ctcgtaaact ttgattctct tttacaagat ccgtcttctg taaacgttca taatcttttt
     8281 taacaataac ggctgcattc actttttctg tcatatctct ccattgctca cgtgtaacca
     8341 caatgtttcc tgttttcttc tcagtaattt ctggctttcc aaacagtttc gttgttactt
     8401 ctgtttttgt ttcttctcgc aaataagaca ttgacactgg cttatccgga atttgatttc
     8461 gaaacgtttc aaattcctgt ttcttttctt ttattttttt atctaaactc aaaaattgtt
     8521 gctgaatttt ttgtaacgtt tcattctgtt tttcgatgta gtttgtggtt tgttgtaatt
     8581 gattatgcgt ttccactact tttgtcttta aatgcgtttt tacattggat aactgttcca
     8641 cttctacttc taaatcttgt ttttccttaa aggcttgagg cgataaatgt tttttctcag
     8701 aaccttctcc ccgatctaca tcaaaacctt tttcccgcat ttccaacggg aagtcctgtt
     8761 gcaaagatat caattctttt ctcgtaaaca tatcttttgc ggacaaacga ttgtcttctg
     8821 taattggcat cacaccaata tgcatatgtg gtgtcgcttc atccatatga accatcgcat
     8881 acaacgtatt ttgtttgcca taacgctttt gaataaaatc tagtgaactc tcaaaataac
     8941 gtttctgttc ttctaaactt aatgaatgca tgtaatcagg cgaagctgtt actacaaatt
     9001 ctgaaagcac aacagaattt gcccgtggct ttcgttttac tcgctcactg atctcttgtt
     9061 taattgtgct ttcatatcta attttttgtt gattcaacaa atcataattc tgttcacttt
     9121 tcgaataatc gatgtctaaa tttgatttac tttttccttg tctttgatta tgtttttgaa
     9181 tcccttgcac atcagaaatt tgaaatttct gcatgtgaat gattgcgaat ttgttcatgt
     9241 ttttccctca caattgtctt ccgtaagagg actactttta aggttatcat aacatatcga
     9301 gagagcgaat ccaaagtttt ggtatagccc actataccaa attacatttg gtgtgggctc
     9361 tgcgagacct cgttggcttc gccaaatcac gccacagcgt gataaagacc aggggcgttg
     9421 ccccacaccc cacaagggga actcttcccc ttgaccccat caatcgaact ccccaacccc
     9481 tcaaatcctt gaggggcttc ctcgccggtt ggcgtcccca aaacggttcg ttttgaggaa
     9541 ctgccaagag gatggaaaat cggctgcacg tgtccattcc ttaatgctcc tttcatagat
     9601 cagcacaaac cgctgatcta ttccagtccg cttaccattg acacttaccg cttatttttc
     9661 ttctcccaat agtcactttg caagttaccc ccatcgctca ttaaatcctt tatcaaactc
     9721 ttaattctaa ttaatactaa attaaaaaaa aataaaaaga ctaattagta cttatctatt
     9781 taatactaat tagtctttat tttatccttt atttaagttc ttttttatac tgtttattat
     9841 tacaaaggtg gatactttaa ttttttcatc tctgtttcag taagtccact tccaccccac
     9901 atttttttct ttatagcttt ttcagaatta aagtaagctt gaaaattaac ttttttatct
     9961 tgataagaat attcataata aatatcagga ttttctcctt caacataaac agcaagccaa
    10021 tatccaccta ttttaaagtc tttacgaaaa tcaatatatt tcaactgact ttcttgtatt
    10081 ccttgtctag caatgtaatt atatatagat tttttcgcat gtactctatt aaataaaaca
    10141 aaactaacta ctaaaactat tgatataaag atagtcatca ttattaacat cttttttttt
    10201 tcaaatttaa tcctcctaaa ttatttatta atatgttttt actaatgacg gtctatatgc
    10261 tgaagtaaaa gtatcaatta aaccataagg agttacgatt ccactaaccg cttgcataac
    10321 atttcttgat ccagaaccaa aataaaatgc ttgaaatact agcttagaac aataagttgg
    10381 atttttttga tacagatgtg tatcgatacc ataatctaaa taaacatttt tagttgcact
    10441 tccagtagtg gaataaaaat ttctatcagc atatctagct acatccctag caagtgtttg
    10501 atcttttaat cgataaacct taatccattt tcctgacgca gtatattttt gcatccaatt
    10561 agatgtagat aattgttgat ttccttggcc agcaccaggc atatgtaaaa tataattatc
    10621 accattagca atagctgcat gtcctactat acctgctgaa cttgttgcat ttgttataaa
    10681 tatatctcca gcttttaaag taaatccacc ccaacttcct tttggagtca catcatcaaa
    10741 aaattcagta tctcccattg gtgcttgtcc ataattattt aattttagcc attcattata
    10801 agataacggt tctagaaaaa caccttgttt taagccatct tgatatatag gcataaattc
    10861 ttctttattt tgttttaacc attcattata agatacacta ttttgatcta aaacaccttc
    10921 ttgaattgct tgattataaa gagattgata atcaataact tcctcagcag caaacgcttt
    10981 caagttaaag ccacaaataa aaaataaaat agcaataaaa ctaatcaata attttttcat
    11041 aaattgtctc cctgaataat tattatattt cggtaacctt ttataaaggt aacacgattt
    11101 tcaaattcaa aatgtaacta cttgtgacaa aaaacataca aatatttact aactaaataa
    11161 tgtaattccc ccttgttttt gggtgtgagt gctggctagt cgtgtggggg ctaaacccca
    11221 catagcgttg atccatatgg aaaaagaatc atgctttgaa cggaagaatt ggcggttcgg
    11281 ctggtacaat tgctagtcca agtcgagcaa cttaaccccg tttttggtct gttcggcttg
    11341 gcgaaagcta aaaaccagcc gaaaaaagtt tggcaaggat gaaacgtagt gaaaaaggtg
    11401 gagatttttg agtgcttttt actcaaaaat acgacctgtt ccttgcggaa cttttagccc
    11461 ttatatagag agtgagagta aacatccctt gtttgaggat aaaaacataa ttatatcaag
    11521 ggtttaagaa ggtttttgaa gttcttaaaa tcaagaacta aagttcttga ttttaagaac
    11581 ttcaaaaata acaaaaaaaa gctgaaaatt tcagctttaa aataatttta caggtaagtt
    11641 ttttaattct tttggaaccc tatgaaacat cgctttcaat gttggattaa catcatcttt
    11701 attacctgca aaaataatat ttggattcat aaaatacgca ttggctctaa cattattacc
    11761 tgcaattcca atctctgaaa cagctaaaat ccctttatct actaatgatt taataatagg
    11821 attaatgtta tttttagttc tttttaacaa taaggcaatc tctgtttgag ttaatggaac
    11881 aggatttttt ttatgtatat catctacaat acaatttgat agaaaaccta tttttctact
    11941 tattttccaa ataaccacaa tttctttgtt atcaagataa tttatttctt ctaattgatc
    12001 tatattatct tgaatgaatt gagcgaattt cactcttgat tgtggagaac gcttagtacc
    12061 tataaaaact tcttttccac cagtagcttt acttaatact tgcattgctt gatttatttc
    12121 ttcttgatta attccgttat gatcttgttt aaacttttct ttttcattct caaaatcacg
    12181 tattcttgca tttttctcaa cttgtttaaa atcaatggta ttggtcatgc ttttctcctc
    12241 gttcctagta tttacaacta gcgaagagac ttttattttg ttattatgta gttagataaa
    12301 ttaattcgag tctgaacact ctaattaatt gtataacagt aagaaaagcc cttctcctat
    12361 agttgtagct tagacaactc aattatagca tgaatggctt tttttgttat atcttgtctg
    12421 actttttata ttccgaaaaa caaatatata cagttttaac taaatttgat atcctaaaaa
    12481 tgtcaataag actaatatct tatttacgcc cattagcaaa aagcccttga tataaaaggg
    12541 ctttttgtca tccatataaa aacaaagata aaataaacag aatattaaaa ataatatata
    12601 tcaacttatt taaaagttaa tctaatgtac taccatgata acatggtaaa aacaagatat
    12661 agataatttt ggaaaagggg aagattctat gaatgttcag ttagaaaatg aacaaataac
    12721 aaaattatta aatgactggt atcaatccat gctaaaacaa caattagtta aagcaaaaca
    12781 attaaaagaa tatatagaca gtgaaattaa caatgtcaaa gaaaatcaaa acttactctt
    12841 atactattct ttattggatt ttagatacaa agcattaacg gattggataa gtattaacga
    12901 aaacagtttt gatgaaatgg ataatttcac aactccagca gatgacttcc tagcttatta
    12961 ttatcacttc tttaaagcat tccattctac tttaacatcc aattacactg aagcaagtga
    13021 acactatgaa aaagcaaaag aactattaat acacattcct gatccaatag aacatgctga
    13081 atttaattat cgaatgggat atttttatta tcaaatctat aaacaagtaa tagctcttga
    13141 ttatataaaa ttagctaaag aagaattttc aaaacatgaa ggttatgaaa taaatatagc
    13201 actatgtgac aactttatag gcctatgttg tattgactta aaacatttcg aactagctga
    13261 ggaatcattt aataaagccc tagacacatt ccaaaaaaat aaccataaaa actctatgct
    13321 tatggtaaga cataatttag cctggttata ttcaaaacaa aatttatcta aactggcaat
    13381 tcgtcatgta tccgaagtaa caaaaaacaa tccaaatcat ttcaaagcat ttttcgtaga
    13441 agcatttgaa tactataaaa taaatgacca taattacgct gaacaactaa ttgaaaaagg
    13501 cttaaaactt agtaacgact ttaacaatca agaattccaa catcgtttta aaatcttaaa
    13561 agcactcaat aataaagttc ctatattaac tttagaaaca tcaatttcag aaggtattac
    13621 ttactttaaa caagaaaaac tatgggaatg tgtaaaagaa tatgctgata tcttagctct
    13681 aagattttat gaagaaaaca accataacaa agcaagtcaa tatttttata tgagcaatac
    13741 agctcaaaaa aacgaactag aaaaaggagc gttaaaataa tgaaaaaagt ttatctaact
    13801 atacttggaa tatctttaat tagtatccta tcgttaggat taaacgactc ttttaaaaat
    13861 caagctgtaa atataggaga cggtggtggt gcacccgcac gacctgatta ttttttacaa
    13921 acacattcta accgataaaa ataaagtata agaaaacacc ttcaaaaaat gaaggtgttt
    13981 tcttatactt tattttttat aaatgaaaaa ctgaatatac caaagttcac taaaccacca
    14041 ttatattaaa attaataaca aaatatgggg tctgtgtagt aatggaacaa gaacggcaat
    14101 aagacttaat catgttatca aaaatcaggg tttttgataa tgataaaaac tgtgttaaat
    14161 taggctttga atcgttaaga ctacgttatc aaaactatta tcataatcaa attaacaata
    14221 acaaccgttt ttgataaatt taacaaatat tgagttttta atcagtaaga tatttatttt
    14281 tttgataaaa aacactctat tattagctat ggtgaagcga aaatataggt tattgccgtt
    14341 tctgttctgt tactacacaa accccaatat taaataaatt caatttcata tttataatta
    14401 tattaattcc acaaaatatc caaacaacaa aaaacagaat tagttaaaaa aactctataa
    14461 atagttccct ttccttttta caaattacaa aaaaaggcca attaaggccc tttaaataat
    14521 cttttaaatc tattaattag ttatatttct ttt
//
